Immobilization of foreing protein into polyhedra of

Bomyx mori nucleopolyhedrovirus

Xing-wei XIANG, Rui YANG, Lin CHEN, Xiao-long HU, Shao-fang YU, Cui-ping CAO, Xiao-feng WU†‡

David Jiménez MarínSara Guzmán RestrepoUniversidad Pontificia Bolivariana School of Health SciencesMarch / 2012

INTRODUCTION

INTRODUCTION

http://staffnet.kingston.ac.uk/~ku33185/Students.html

http://www.ent.iastate.edu/dept/faculty/bonningb/node/53

INTRODUCTION

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INTRODUCTION

INTRODUCTION

GENERAL OBJECTIVE

GENERAL OBJECTIVE

Check the Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus (BmNPV) starting from the creation of two recombinant baculoviruses.

MATERIALES Y MÉTODOS

MATERIALES

MATERIALES

MATERIALES Y MÉTODOS

MATERIALES Y MÉTODOS

MATERIALES Y MÉTODOS

MATERIALES Y MÉTODOS

MATERIALES Y MÉTODOS

MATERIALES Y MÉTODOS

MATERIALES Y MÉTODOS

http://betbettybea.blogspot.com/2011/11/electroforesis.html

MATERIALES Y MÉTODOS

MATERIALES Y MÉTODOS

MATERIALES Y MÉTODOS

RESULTADOS

Figura 1

Figura 2

Figura 4

Figura 5

DISCUSSION

DISCUSSION

AUTHOR OPINION AGREEMENT DISAGREEMENT

Reid and Flynn

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is stable between pH 5–12, and displays visible fluorescent light at a peak wavelength of 508 nm when excited with ultraviolet light.

X

Ikeda

According to the previous research, EGFP alone cannot resist the stimulation from dehydrated treatment, whereas the dehydrated treatment had little effects on EGFP immobilized into polyhedra.

x

AUTHOR OPINION AGREEMENT DISAGREEMENT

Blissard and Rohrmann

In this study, we confirmed that the foreign protein could be embedded into the polyhedra. However, the process of virion occlusion in the polyhedra is undefined and the genes that regulate the virion occlusion process have not been well investigated

X

Mori

Showed that the BmCPV polyhedrin can be assembled into polyhedra in the nucleus of Sf21 and BmN cells using the baculovirus expression vector.

X

DISCUSSION

CONCLUSIONS

CONCLUSIONS

Of this study we can conclude that recombinant viruses work the same way as the control virus, because there wasn't differences in the number, shape and size of the polyhedra between recombinant viruses and control virus.

This study may serve as a basis for further studies. for example, to study how much of protein can be immobilized in polyhedra or how are released the proteins of the polyhedra for vaccine development?

CONCLUSIONS

The technique of SDS PAGE and western blot analysis is needed to confirm the results of the study because it shows the interaction between the antibody and the protein of interest.

Is important to know these viruses because they can be applied in medicine or agriculture for the creation of different products based on them.

BIBLIOGRAPHY

BIBLIOGRAPHY

• Ikeda, K., Nakazawa, H., Shimo-Oka, A., Ishio, K., Miyata, S., Hosokawa, Y., Matsumura, S., Masuhara, H., Belloncik, S., Alain, R., et al., 2006. Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays. Proteomics, 6(1):54-66.

• http://www.gusanet.es/alternative.html

• Xiang, X., Yang, R., Yu, S., Cao, C., Guo, A., Chen, L., Wu, X., Cui, W., Cenis, J.L., 2010. Construction of a BmNPV polyhedrin-plus Bac-to-Bac baculovirus expression system for application in silkworm, Bombyx mori. Appl. Microbiol. Biotechnol., 87(1):289-295.

• http://www.agrobiologicossafer.com/baculovirus.htm

MAPAS CONCEPTUALES

DAVID JIMENEZ

SARA GUZMAN

THANKS…