Laura Montoya TrejosFelipe Hernández Restrepo

Teacher Lina MartínezMedicine Faculty

III Semester

STAPHYLOCOCCUS

Is resistant to penicillin, and this has become a major challenge for the medical community.

Staphylococcus aureus can kill heart failure due to endocarditis

STAPHYLOCOCCUS AUREUS

MICROBIOTA

It can be defined as microorganisms that are often found in various parts of the body, associated with normal tissues skin and mucosa of the human body in healthy individuals

These help in the digestion of food, produce vitamins and protect against colonization by other microorganisms which may be pathogenic

ELECTROPHORESIS

Is called electrophoresis technique whereby the biomolecules in solution are separated when they are subjected to an electric field.

The separation is given by the electric charge and the molecular weight

PCR

GENERAL OBJECTIVE

The aim of this work was to optimize the study of Staphylococcus species diversity in human breast milk and neonate stool.

MATERIALES Y METODOSCEPAS BACTERIANAS

MATERIALES Y METODOSPACIENTES

1

2

MATERIALES Y METODOSEXTRACCION DEL DNA

FUNDAMENTO DE LA EXTRACCION DEL DNA

EXTRACCION DEL ADN

1. Lisis celular

2. Precipitación de proteínas

3. Precipitación de DNA

4. Almacenamiento

Precipitación del RNA (RNAsa)

MATERIALES Y METODOSPCR

FUNDAMENTO PCR

1. Desnaturalización del DNA para dar hebras sencillas

2. Hibridación especifica de la hebra sencilla con un oligonucleotido

3. Replicación de la hebra sencilla por una DNA polimerasa a partir del olignonucleotido anterior como cebador

Se realizo esto para amplificar el gen tuf, se hizo por medio de 3 pares de primers

MATERIALES Y METODOSELECTROFORESIS

Es una técnica mediante la cual se separan las biomoléculas en disolución cuando se ven sometidas a un campo

eléctrico.

Electroforesis en gel de gradiente de temperatura utiliza ya sea una

temperatura o gradiente químico para desnaturalizar la muestra

cuando se mueve a través de un gel para separar acidos nucleicos

MATERIALES Y METODOSELECTROFORESIS

Los RFLP son marcadores genéticos del ADN y se pueden encontrar en regiones que codifican proteínas o exones, en los intrones o en el ADN que separa un gen de otro.

MATERIALES Y METODOSFRLP

La técnica RFLP se usa como marcador para identificar grupos particulares de personas con riesgo a contraer ciertas

enfermedades genéticas, en ciencia forense, en pruebas de paternidad.

MATERIALES Y METODOSSECUENCIACION

RESULTADOS

Ejemplo

•De las 18 solo se discriminaron 15 bandas.

Se discriminaron con RFLP:

•S. Haemolyticus (182 y 139pb) - S. Scheiferi (no) (Bmpl)

•S. Cohnni (160 y 161pb) – S. Capitis (no) (Folk)

•S. Caprae (140, 114, 53,13pb) – S. Lugdunensis (140 y 180pb) (Acul)

DISCUSSION

AUTHOR WHAT HE SAID YES/NO

Ghebremedhin, B 16S rRNA gene is insufficient to identify Staphylococcus species and provides identification only to the genus level or for a group of species in the genus.

YES

Dahllöf, Drancourt and Raoult

The rpoB gene, proposed for community analyses, has classically been used for the identification of staphylococci because it is more discriminative then the 16S rRNA gene among staphylococci.

NO

1. This research showed that if all the hospitals in the world make this exam to all of the neonates, we could prevent the propagation of the agent.

2. The tuf-PCR TTGE exam is very effective and faster than the culture, which facilitates the attention in the hospitals.

3. However culture its very important because it allowed us to iddentificate other species that the PCR did not iddentificate.

4. The diagnosis is very important because it could save the baby’s life and make him not to suffer the consequenses of having the agent inside of him.

THANK YOU