46
September 2018 SHAPING THE FUTURE OF BIOENERGY AND RENEWABLE CHEMICALS
September 2018
SHAPING THE FUTURE OF
BIOENERGY AND RENEWABLE
CHEMICALS
METGEN AT A GLANCE
• Tailor-made industrial enzymatic solutions
• Energy, renewable chemicals and pulp&paper sectors
• Product performance validated by customers
• Strong IP portfolio
• Lean business model & low break-even point
• Wide collaboration network & preferred enzyme developer in
EU Horizon 2020 projects
METGEN STRATEGIC FOCUSLignocellulosic biomass conversion in multiple segments
PULP AND PAPER
RENEWABLE
CHEMICALS
BIOFUELS & ENERGY
BIOMATERIALS
VOLUME OF
CELLULOSIC
BIOMASS
Biobased
plastics
Energy, Liquid
fuels & Biogas
Lignin-based materials
Nanocellulose
Packaging,
Tissue, Paper
WASTE WATER &
SIDE STREAMS
Clean water, biogas &
Chemical building blocks
and Biorenewable
TARGETED MARKETS
Up to M€ 3 / mill through:
• Energy and cost savings
• Product quality & process improvements
• Sustainability improvements
Making industry sustainable by:
• Lowering technology costs
• Lowering CAPEX
• Enabling new & better products
Biorefineries and
Renewable Chemicals
Pulp, Tissue, and Packaging
NEW BUSINESS DEVELOPMENT
Improving biogas production by:
• Reducing inhibition of
feedstock, waste and
operational cost
• Better utilization of raw material
• Raw material substitution
Enhancing water treatment by:
• Reducing inhibitors, waste, and
operational cost
• Lowering soluble phenols &
hard COD
• Reducing micro pollutants
Waste Water TreatmentBiogas
ENZINE® TECHNOLOGY PLATFORM
• FAST: Industry leading short
development cycle
• FLEXIBLE: Tailored enzyme design
approach to meet challenging
industrial conditions
• ADAPTABLE: Can produce large
amounts of different enzymes, cost-
effectively and supply globally
• PROVEN: Developed several novel enzymes from concept to validation at industrial scale and production
Unique
Combination
Identified
Customer
Challenge
Piloting and
scale-up
Technical
support
Industrial
supply of
enzyme
Designing,
cloning
and
screening
Application
testing
UNIQUE COMBINATION OF CAPABILITIES
Incumbent
companies
Enzyme
ResellersResearch Tollers
Tailored
Solutions
Protein
Engineering
Process
Development
Industrial
Production
METZYME® PRODUCT FAMILIES
FOR PULP AND PAPERA
pp
lic
ati
on
Ben
efi
ts
En
zy
me
Ad
de
d t
o
Pro
du
ct
Fa
mil
ies
LIGNOTM BRILATM POVONTM PLATATM SEKALOTM
LIGNIN
MODIFICATION
RECYCLED
FIBRE
FLUTING
LINERPITCH CONTROL PAPER COATING
WOOD CHIPS
REJECT PULP
KRAFT PULPING
LIGNIN
RCF
DEINKED PULPCHEMICAL PULP PULP STARCH
Yield
improvement,
Efficiency and
Energy Saving
Quality & Process
Improvements
Energy & Raw
Material Savings
Quality & Process
Improvements
Energy & Raw
Material Savings
Break–down of
aggregated
impurities &
reduction of
process upsets
Economic
Starch Conversion
METZYMES® COVER THE FULL
VALUE-CHAIN IN BIOPROCESSING
Unique feedstock and pre-
treatment combinations
• Tailored enzymatic
solutions are a MUST for
processing biomass
Substrate
Utilization of sugars
Further Conversions
Sugar platform
• High Yields
• Affordable sugars
• High dry-solid %
• Lower capex
Fermentation
• Less inhibitors
• High efficiency
• Higher carbon
yield
Chemical building blocks
• Furans and aromatics
• Lignin-based materials
• Platform sugars
• Streamlined processes
Side-streams and environment
Water & biogas
• Less COD & waste
• Efficient processes
• Neutralizing toxins
LIGNO™ SUNO™ PURECO™ FORICO™
FORCI™
Hydrolysis and Fractionation
RECYLED FIBRE (RCF) & DEINKED PULP
STRENGTH IMPROVEMENT
Challenge Unplanned downtime machine costs around 1 M€ / year
Solution MetZyme® BRILA™ improves product properties
Results
Product breakage decrease up to 60% and downtime
reduced by 50%,
Enables substitution of virgin fibre with RCF
Value Proposition Net Savings to Mill 3 M€ / year
Reference Commercial customers
MetZyme® BRILATM
dosed on RCF or
Virgin fibre
Tissue or Board
machine
NEUTRAL SULFITE SEMI CHEMICAL PULP (NSSC)
STRENGTH IMPROVEMENT
ChallengePaper machine runability issues
Unplanned downtime machine costs around 1 M€ / year
Solution MetZyme® POVON™ improves strength properties
ResultsPaper machine web-breaks decreased up to 60%,
increased production rate and energy savings
Value Proposition Net Savings to Mill 1 M€ p.a
Reference Commercial customers
MetZyme®
POVONTM dosed on
NSSC
Fluting paper
machine
PULP PROCESSING - ENERGY SAVING
ChallengeAverage mill spends 20 M€/year on energy, refining is 50%
of the electricity bill
SolutionReduce energy consumption by treating wood chips with
MetZyme® LIGNOTM
Results20% less electricity consumption and final product strength
increased 10-15 %
Value Proposition Net Savings to Mill operator 1.5 M€ / year
Reference Industrial Mill trials
Wood chips
treated with
MetZyme® LIGNOTM
Pulp production in
refiners
METZYME® PRODUCT FAMILIES
FOR BIOREFINERIESA
pp
lic
ati
on
Ben
efi
ts
En
zy
me
Ad
de
d t
oP
rod
uct
SUNOTM PURECOTM
HYDROLYSIS AND SACCHARIFICATIONCONVERSION OF SUGARS TO VALUE-
ADDED CHEMICALS
PRE-TREATED CELLULULOSIC BIOMASS LIGNOCELLULOSIC SUGARS
High sugar yield at higher dry-matter content,
reduced time, lower enzyme cost and more
flexible processing conditions
Enzymes do not have to be expensive!
Novel enzymes enable affordable renewable
chemicals.
New industrial applications are enabled through
tailored enzyme characteristics:
e.g. substrate specificity, pH, temperature,
solvent, and inhibitor tolerance.
BIOREFINERIES - CELLULOSIC SUGARS
Challenge
High enzyme cost
Inhibition at high consistency of biomass
High capital and operating cost
SolutionMetZyme® SUNO™ Tailor-made drop-in solution for
hydrolysis and saccharification
Results
Sugar yield increase or enzyme dosing decrease
Biomass dry-matter consistency increased to >20%
More compact processes and lower CAPEX
Value PropositionEnabling competitive usage of cellulosic biomass against
1st generation sugars
ReferencePilot scale & H2020 Projects BIOrescue and ButaNexT
Industrial demonstration in SWEETWOODS Flagship Project
MetZyme®
SUNOTM added to
pretreated biomass
Conversion to
sugars
METZYME® SUNO™ - STRONG SOLUTION FOR
HYDROLYSIS AND SACCHARIFICATION
EVOLUTION OF ENZYME COST IN
CELLULOSIC SUGAR PROCESSES
€-
€0,05
€0,10
€0,15
€0,20
€0,25
€0,30
€0,35
€0,40
€0,45
€0,50
ModifiedExtrusion /Hardwood
Hydrothermal /Hardwood
Hydrothermal /Straw
Acid StEx /Hardwood
Soda cooking /Hardwood
BALI process /Softwood
Enzyme cost €/ kg of reducing sugar
Non-tailored enz 2018 2020 2022 w/ Integrated Enz manufacturing
BIOREFINERIES – RENEWABLE BUILDING
BLOCKS ENABLING BIOPLASTICS
ChallengeHigh enzyme cost with challenging lignocellulosic biomass
Commercial enzymes are inhibited by biomass
Solution MetZyme® PURECO™ Glucose Isomerase
Results
Isomerization in non-purified hydrolysates
Better stability, yield increase and total productivity increase
Streamlined processes and lower CAPEX & OPEX
Value Proposition Enabling technology for lignocellulosic plastics
Reference
Pilot scale
H2020 projects ReTAPP, BIOFOREVER and
SWEETWOODS
MetZyme®
PURECOTM
glucose isomerase
added to
hydrolysate
Conversion to
fructose
PLATFORM
CHEMICALS
STREAMLINED CHEMO-ENZYMATIC ROUTE
TO PLATFORM CHEMICALS
MetZyme®
PURECO™2nd Gen
GlucoseFructose
Isomerases
MetZyme®
PURECO™
CHEMICAL
CONVERSIONS
BIO-
CONVERSION
FDCA
1st & 2nd
Gen
Glucose
Other
Sugar(s)
Dehydration
Oxidation
Oxidases
Dehydration
Oxidation
etc.
Heterogenous /
Solid catalysts
MetGen’s patent-filed processes enable:• Complete C6 conversion
• Use of 2nd Gen sugars
• Streamlined processes and higher yields
• New routes to platform chemicals
LIGNIN POLYMERIZATION
Challenge
Hemicellulose is often after pre-treatment contaminated with
lingosulfonates or other monomeric lignols rendering sugar
unusable for fermentation
Market
Opportunity
Increases the overall economics of biorefineries, enables
further valorization of C5 sugars to renewable chemicals
Desired EffectFiltration is easily and energy efficient,
C5 sugars are purified and valorized
SolutionMetZyme® LIGNO™ polymerizes the lignin-based monomeric
and colloidal particles
Status and next
stepsPilot with collaboration partner
MetZyme® LIGNOTM
added to C5 sugars
Lignosulfonates
are polymerized
and filtered away
from sugar stream
CLEAN FRACTIONS HAVE PLENTY OF USES
CLEAN LIGNIN:
• Foams: polyurethanes, expanded polystyrene, starch
• Biopolymers: resins, polyols, binders
• Packaging materials: fillers, biodegradable components
• Activated carbon
• Carbonfiber
METZYME® LIGNO™ FOR BIOREFINERIES:
LIGNIN MODIFICATION / VALORIZATION
Challenge
Economics of modern Biorefineries require commercial lignin
utilization (other than burning). Complex molecule needs to be
cleaved to smaller, more specific and reactive fractions.
Market
Opportunity
Economically viable drop-in substitute for oil-based chemicals
in binders, fillers, foams, plasticizers, coatings, etc.
More defined aromatic compounds open up new chemistries.
Desired EffectAffordable lignin fractionation process for smaller molecules in
water-soluble conditions
SolutionNovel and unique MetZyme® LIGNO™ with operational
optimum of pH 10-11 and thermostability up to 80 °C
Status and next
steps
Patents filed, Pilot scale development on-going, first license
sold. Demo scale & application testing next
MetZyme® LIGNOTM
added on soluble lignin
Lignin is “split” to
smaller molecule
size compounds
ACTION OF METZYME® LIGNO™ ON LIGNIN
l. Munk et al., 2015. Biotechnology advances 33;13-24.
ACTIVATION OF LIGNIN STRUCTURE Electron withdrawal from subunit in lignin
Increased reactivity
DepolymerizationConsecutive cleavage
Decrease in average molecular
weight & release of lignin
substructures
Modification
Change in functional group
Acetylation and demethylation
Grafting
Single attachment for
changes in properties
(e.g. Solubility)
PolymerizationContinuing attachment
Increase in average molecular
weight & capture of lignin
substructures
Bond CleavegeElectron withdrawal from
subunit in lignin
Increased reactivity
CouplingAttachment of
phenylpropanoids
and/or
Mediators
MetZyme® PURECO™ IMPACT ON LIGNIN
*Hämäläinen et al. “Enzymatic process to unlock the lignin value” Frontiers in Bioengineering and Biotechnology -Bioenergy and Biofuels, 2018, 10.3389/fbioe.2018.00020
METZYME® PURECO™ IMPACT ON LIGNIN
Stable depolymerisation without
repolymerisation
Operations in aqueous medium enables
membrane operations to collect specific fractions
*Hämäläinen et al. “Enzymatic process to unlock the lignin value” Frontiers in Bioengineering and Biotechnology -Bioenergy and Biofuels, 2018, 10.3389/fbioe.2018.00020
MetZyme® PURECO™ fractionates the bulk of industrial lignin at
fully soluble conditions @ pH 11 – in water without a mediator
MetZyme® PURECO™ IMPACT ON
LIGNIN CHARACTERISTICS
MetZyme® PURECO™ IMPACT ON
LIGNIN CHARACTERISTICS
Commercial
lignin
Biorefinery
Lignin
ENZYMATIC LIGNIN FRACTIONING
Solubilization
Solids
separation
Enzymatic
Fractionation
UF
NF
Enzyme+
Water
NaOH
Mixing
Aeration
Water/NaOH
Recycling
Low molecular
weight lignin
60%
Medium molecular
weight lignin
25%
Unsoluble lignin
15%
85%
Water
NaOH
Mixing
Heating
LIGNIN SAMPLES AT VARIOUS PROCESS STEPSSeparations performed on soluble lignin using 10 kDa and 2 kDa ultrafiltration membranes
Note: molecular weight may be much smaller than the membrane nominal cut-off
METGEN’S PROCESS IMPACT ON LIGNIN
Rapid polymerisation (in minutes)
• Can be controlled with pH
• Will happen in non-controlled process
Controlled depolymerisation in continuous process
• Small mw particles removed from reaction
• Allows for more efficient depolymerization
CONDITIONS IMPACT ON FRACTIONS
Conditions allow for specific fractionation with membranes
This peak
corresponds
to trimers
Dimers &
monomers
ENZYMATIC LIGNIN FRACTIONING
The highly complex structure, unspecified and too high molecular weight have been a major challenge in lignin utilization.
Enzymatic fractionation allows for high yield of specific low molecular weight fractions
LIGNIN MASS DISTRIBUTION BY FRACTION
Molecular
WeigthDissolved Lignin
Enzyme
depolymerized
Lignin
5–100 kDa 7 % 0 %
1–5 kDa 90 % 32 %
0,3–1 kDa 3 % 68 %
LIGNIN DEPOLYMERIZATION ADDS VALUE
Molecular
Weigth
Reactivity &
SolubilityApplication
Bio-
Equivalent
of
Price
Currently
available lignin5–100 kDa Poor Burning
Oil /
Electricity
50 - 100
€/ton
Dissolved
lignin3–50 kDa Medium
Resins &
Adhesives
Form-
aldehyde
400 – 500
€/ton
Enzyme
depolymerized
lignin0,3–2 kDa Good
Foams &
CompositesPVA
1000 – 1500
€/ton
Enzyme
depolymerized
fractions
0,3 kDa
0,5 kDa
0,7 kDa
... 2 kDa
Very GoodNew
materials
Specialty
Chemicals
&
Polymers
> 1500
€/ton
METZYME® PRODUCT FAMILIES
FOR WATER TREATMENT AND BIOGASA
pp
lic
ati
on
Ben
efi
ts
En
zy
me
Ad
de
d t
oP
rod
uct
FORICOTM FORCITM
WASTE WATER / EFFLUENT TREATMENT BIOGAS
EFFLUENT FEEDSTOCK / PROCESS WATER
Removes and neutralizes the negative effect of
residual peroxide, lignin and phenolic
compounds in effluent
Up to 20% improved capacity of microbial waste
water treatment
Addresses lignin and feedstock recalcitrance for
better utilization of nutrients
Up to 90% improvement in methane production
Up to 70% reduction in COD levels
10% less waste
SIDESTREAMS – WATER / EFFLUENT
ChallengeResidual peroxide inhibits water treatment and biogas
processes downstream
SolutionMetZyme® FORCI™ quickly and thoroughly remove the
residual chemicals
ResultsBiogas production increase, preservation of the reactor when
high peroxide load is used
Value PropositionEnables efficient effluent treatment, such as biogas
production
Reference Commercial & Industrial Customer
MetZyme®
FORICOTM added
to effluent
Removal of
residual peroxide
BIOGAS
ChallengeLignin and other phenolic compounds inhibit microbial
process and recalcitrant biomass resists giving up nutrients
SolutionMetZyme® FORCI™ enzyme solutions to address inhibitors
and recalcitrant biomass
ResultsLignin oxidation and soluble lignin polymerization
Methane production increase up 90 %
Value PropositionMoro thorough usage of biomass resulting in increased
methane production, lowered COD and less waste
Reference Pilot scale & 700 ton straw batch reactor
MetZyme®
FORCITM added to
biomass /
process water
Improved process
and methane yield
with less waste
TECHNOLOGY READINESS LEVELS (TRL)
LIGNO™ SUNO™ PURECO™ FORCI™ FORICO™
TRL 8-9
Energy saving in
refining
Improved fibre quality
Better extraction of
xylose
High sugar yield from
lignocellulosic
substrates
Tailored solution for
each unique substrate
Lignin
depolymerization and
activation
Glucose conversion
towards bioplastics
and platform
chemicals in crude
hydrolysate
Addressing lignin and
phenolic compounds
resulting in higher
methane output
Increasing
carbohydrate
availability
Lowering viscosity
Removing residual
peroxide from
industrial effluents
Adressing lignin and
phenolic related
issues in water
streams
TRL 5-7
Delignification in Kraft
processes
Energy saving and
yield improvement in
nanocellulose
processes
Lignin polymerization
for purification of side
streams
Enzyme for affordable
Nanocellulose
High yield hydrolysis
@ >25 % dry-solids
Detoxification of hard
substrates
New solutions
combining enzyme
and pretratment
technologies
Enzymatic HMF
oxidation to FDCA
One-pot enzymatic
xylose conversion to
xylitol with reduced
number of cofactors
and unified process
conditions
Immobilized enzyme
systems
Tailored solutions for
different substrates
and reactors for
increase production,
cleaner gas, less
waste and lower
COD.
Adressing triglyserids
and lipids to help
microbial processes
Combined solution for
addrssing chemicals
and lignin-based
inhibitors for lower
COD, less waste and
high efficiency
TRL < 4
Extractives control < 100$ / ton of
ethanol equivalent
enzyme cost
Direct sugar
conversion to
chemical building
blocks for e.g. HMF,
FDCA
Complete solutions
designed in
collaboration with
equipment
manufacturers
Complete Chemo-
enzymatic solutions in
collaboration with
companies focusing
on water chemistries
ON-GOING H2020
DEVELOPMENT PROJECTS
2nd GEN BIOREFINERY
LIGNIN
STRAW & GRASS
BIOWASTE
MARINE
DIESEL
BUTANOL
SUGAR
PLATFORM
FORESTRY
FEEDSTOCKS
SOFT & HARD WOODRENEWABLE
PLASTIC
CHEMICAL
BUILDING
BLOCKS
GLUCOSE ISOMERASE +
HYDROLYSIS ENZYMES
INHIBITOR RESISTANT
GLUCOSE ISOMERASE
SOLVENT TOLERANT
INDUSTRIAL LACCASE
DETOXIFICATION + LIGNOLYTIC
ENZYMES + HYDROLYSIS
SOLUTION
DETOXIFICATION + LIGNOLYTIC
ENZYMES + HYDROLYSIS
SOLUTION
57 PARTNERS IN 9 EU PROJECTS WITH TOTAL OF 90 M€
INTEGRATED
HARDWOOD
BIOREFINERY
BIOCHEMICALS
BIOMATERIALS
HYDROLYSIS
CONVERSION OF SUGARS
FRACTIONATED LIGNIN
80tons / day
Sustainable hardwood biomass
SWEETWOODS PROJECT
LSCA: 2B
Technology viability: Vertech
Project management: Spinverse
LIGNIN
SUGARS
SWEETWOODS is a 43-million-euro joint Integrated
Biorefinery Flagship Project that was recently granted 21
million euros in funding under the EU’s Horizon 2020
programme’s Bio-Based Industries financial instrument.
END-USE
•Polyurethane foams
•Elastomeric foams
•Biocomposites
•Xylitol
•2G Ethanol
•Biocomposites
•Plywood resin additive
•Bitumen additive
•Renewable Platform
chemicals, including
bioplastics such as
•PEF,
•PBF and
•p-xylene
Also, 100% conversion
of glucose to fructose
C5
LIGNIN
SWEETWOOD PRODUCTS
• Bio-IBN, PLA
Glucosone
Depolymerized
LIGNIN
Fructose
BALTIC FOREST RESIDUE HARDWOOD
RAW MATERIAL
C6
ACKNOWLEDGMENT OF PUBLIC EU H2020 FUNDING
SWEETWOODS (792061), WoodZymes (792070), UNRAVEL (792004), BIOrescue (720708) and BIOFOREVER
(720710) have received funding from the Bio Based Industries Joint Undertaking, Industrial Leadership project
FALCON (720918) under the European Union’s Horizon 2020 research and innovation programme
Gradle-to-Gate LCA on enzymatic solutions
Enzymes by leading commercial brand
MetZyme® LIGNO™
SUMMARY
• Commercial solutions for P&P, renewable chemicals and materials, water
treatment and biogas
• Strategic focus on lignocellulosic biomass
• Unique, tailored enzymatic solutions that address customer needs
• Industry-leading short development cycles
• Solutions are protected by strong IP portfolio
• Lean business model
• Preferred enzyme developer in EU Horizon2020
with 9 projects, 57 partners, and >12 M€ public financing over last 5 years
• Wide collaboration network and access to top technologies
MANAGEMENT TEAM
Alex MichineCEOSerial entrepreneur, Ex-PerkinElmer
Sami-Pekka RantanenSales DirectorEx-Buckman
Matti HeikkiläCTOEx-DuPont
Klara BirikhR&D DirectorEx-Oxford Genetics Laboratory
Board of Directors
Martin Granholm, M.Sc,
D.Sc (hc)
Chairman
Former Deputy CEO of
UPM-Kymmene
35 years in several
positions in the Forest
Industry, member and
chairman in the boards of
various organizations
Positions in the Chamber
of Commerce
organization both locally
and globally.
Joško Bobanovic, PhD,
MBA
Board member
Partner of the Sofinnova
Green Seed Fund
13 years in venture
capital with Sofinnova
and iNovia Capital.
Board member of
portfolio companies
Gina Domanig, B.Sc,
MBA
Board member
Over 25 years of
international business
Managing partner of
Emerald Technology
Ventures. Currently
serves on the boards of
GeoDigital International
Inc, MetGen Oy, Ushr Inc
and TaKaDu Inc.
Steven Govoni, PhD
Board member
Vice President, RD&E for
Ecolab Inc. Responsible
for the global direction of
Nalco since 2008. Lead
of Reuse of Process
Effluents Team within the
Agenda 2020
Technology Alliance.
page 46Confidential
Equity investors
HST Partners Oy
Committed Funding Partners
Government
