C190-E221
Shim-pack Scepter LC ColumnsExcellent stability & performance could be achieved under a wide range of LC conditions
Method Scouting Utilize excellent stability & performance under a wide range of LC conditions
Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications. With different particle sizes (1.9 µm, 3 µm, 5 µm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.
Shim-pack Scepter Chemistries
Shim-pack Scepter
Ligand Type
Particle
Particle Size
Pore Size
End Capping
pH Range
100% aqueous condition
USP Classification
Trifunctional C18 Trifunctional C18
Generic Purpose Type High Density Type
12 nm 8 nm
Proprietary
1 - 12
No
L1
No
L7
Yes
L1
1 - 10
Yes
L11
1 - 8
Yes
L43
2 - 10
N/A
L20
None
12 nm
Trifunctional C8Trifunctional Phenylbutyl
Trifunctional Pentafluorophenylpropyl
Trifunctional�Dihydroxypropyl
Organic Silica Hybrid
1.9 µm, 3 µm, 5 µm
Reveresed Phase
C8C18 HD-C18 Phenyl PFPP
HILIC
Diol-HILIC
pH2.9
pH6.1
pH9.9
C8C18 HD-C18 Phenyl PFPP
With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting combining mobile phase pH and organic modifier.
Analytes
1. Saccharin (pKa = 2.2)
2. Dextromethorphan (pKa = 8.3)
3. Amitriptyline (pKa = 9.4)
4. N-Butyl paraben (pKa = 8.3)
5. Ibupurofen (pKa = 4.4)
Comparison of Chromatograms using Gradient condition with Acetonitrile
Outside of recommended pH
mAU
0
20
min0 2 4 6 8 1210
1
2 3 4 5
mAU
0
20
min0 2 4 6 8 1210
1
2 3 5 4
F140812A
mAU
0
20
min0 2 4 6 8 1210
1
2 3 4
5
mAU
0
20
min0 2 4 6 8 1210
2
3 4
5
1
mAU
0
20
min0 2 4 6 8 1210
4 5
1 ( peak 2, 3 : N.D.)
mAU
0
20
min0 2 4 6 8 1210
1
2 3,
4
5
mAU
0
20
min0 2 4 6 8 1210
1
2
3
5 4
mAU
0
20
min0 2 4 6 8 1210
1
2
3
4 5
mAU
0
20
min0 2 4 6 8 1210
1
2 3
4
5
mAU
0
20
min0 2 4 6 8 1210
1
2 3
4 5
mAU
0
20
min0 2 4 6 8 1210
1
2 3
4
5
mAU
0
20
min0 2 4 6 8 1210
1
2 3
4
5 mAU
0
20
min0 2 4 6 8 1210
1
2
3 4
5
mAU
0
20
min0 2 4 6 8 1210
1
2
3 4
5
Shim-pack Scepter Analytical Columns
Shim-pack Scepter Preparative Columns
Particle Size (µm)Chemistry C18 HD-C18 C8
Ordering Information
* Main P/Ns are described in the list. Please contact your local representative for guard columns and columns in dimensions other than described above.
ID (mm)� 2.1227-31012-03227-31012-04227-31012-05227-31012-06227-31014-03227-31014-04227-31014-05227-31014-06
227-31017-03227-31017-04227-31017-05227-31017-06
3227-31013-01227-31013-02227-31013-03227-31013-04227-31015-01227-31015-02227-31015-03227-31015-04
227-31018-01227-31018-02227-31018-03227-31018-04
4.6
227-31016-02227-31016-03227-31016-04227-31016-05227-31016-06227-31020-02227-31020-03227-31020-04227-31020-05227-31020-06
4.6
227-31030-02227-31030-03227-31030-04227-31030-05227-31030-06227-31024-02227-31024-03227-31024-04227-31024-05227-31024-06
2.1227-31026-03227-31026-04227-31026-05227-31026-06227-31028-03227-31028-04227-31028-05227-31028-06
227-31021-02227-31021-03227-31021-04227-31021-05
3227-31027-01227-31027-02227-31027-03227-31027-04227-31029-01227-31029-02227-31029-03227-31029-04
227-31022-01227-31022-02227-31022-03227-31022-04
4.6
227-31037-02227-31037-03227-31037-04227-31037-05227-31037-06227-31041-02227-31041-03227-31041-04227-31041-05227-31041-06
2.1227-31033-03227-31033-04227-31033-05227-31033-06227-31035-03227-31035-04227-31035-05227-31035-06
227-31038-03227-31038-04227-31038-05227-31038-06
3227-31034-01227-31034-02227-31034-03227-31034-04227-31036-01227-31036-02227-31036-03227-31036-04
227-31039-01227-31039-02227-31039-03227-31039-04
507510015050751001502505075100150250
1.9
3
5
Length (mm)
ID (mm)�Length (mm)Particle Size (µm)Chemistry Phenyl PFPP Diol-HILIC
2.1227-31063-03227-31063-04227-31063-05227-31063-06227-31065-03227-31065-04227-31065-05227-31065-06
227-31068-03227-31068-04227-31068-05227-31068-06
3227-31064-01227-31064-02227-31064-03227-31064-04227-31066-01227-31066-02227-31066-03227-31066-04
227-31069-01227-31069-02227-31069-03227-31069-04
4.6
227-31067-02227-31067-03227-31067-04227-31067-05227-31067-06227-31071-02227-31071-03227-31071-04227-31071-05227-31071-06
4.6
227-31057-02227-31057-03227-31057-04227-31057-05227-31057-06227-31061-02227-31061-03227-31061-04227-31061-05227-31061-06
2.1227-31053-03227-31053-04227-31053-05227-31053-06227-31055-03227-31055-04227-31055-05227-31055-06
227-31058-03227-31058-04227-31058-05227-31058-06
3227-31054-01227-31054-02227-31054-03227-31054-04227-31056-01227-31056-02227-31056-03227-31056-04
227-31059-01227-31059-02227-31059-03227-31059-04
4.6
227-31047-02227-31047-03227-31047-04227-31047-05227-31047-06227-31051-02227-31051-03227-31051-04227-31051-05227-31051-06
2.1227-31043-03227-31043-01227-31043-02
227-31045-03227-31045-04227-31045-05227-31045-06
227-31048-03227-31048-04227-31048-05227-31048-06
3227-31044-03227-31044-01227-31044-02
227-31046-01227-31046-02227-31046-03227-31046-04
227-31049-01227-31049-02227-31049-03227-31049-04
507510015050751001502505075100150250
1.9
3
5
Chemistry 10
227-31101-01227-31101-02
227-31104-01227-31104-02
227-31107-01227-31107-02
20227-31102-01
227-31102-02227-31102-03227-31102-04227-31105-01
227-31105-02227-31105-03227-31105-04227-31108-01
227-31108-02227-31108-03227-31108-04
30227-31103-01227-31103-02227-31103-03227-31103-04227-31103-05227-31106-01227-31106-02227-31106-03227-31106-04227-31106-05227-31109-01227-31109-02227-31109-03227-31109-04227-31109-05
10
227-31113-01227-31113-02
227-31110-01227-31110-02
Chemistry
Phenyl
PFPP
20227-31114-01
227-31114-02227-31114-03227-31114-04227-31111-01
227-31111-02227-31111-03227-31111-04
30227-31115-01227-31115-02227-31115-03227-31115-04227-31115-05227-31112-01227-31112-02227-31112-03227-31112-04227-31112-05
507510015025050751001502505075100150250
C18
HD-C18
C8
50751001502505075100150250
ID (mm)�Length (mm)ID (mm)�Length (mm)
© Shimadzu Corporation, 2018
www.shimadzu.com/an/
For Research Use Only. Not for use in diagnostic procedures. This publication may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.Company names, products/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation, its subsidiaries or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services, whether or not they are used with trademark symbol “TM” or “®”.Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.
First Edition: September 2018, Printed in Japan 3655-09807-20AIK