PPSQ-31A/33A

ShimadzuProtein Sequencer

C297-E055A

Printed in Japan 3295-12801-10ANS

The contents of this brochure are subject to change without notice.

JQA-0376

SHIMADZU CORPORATION. International Marketing Division3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan Phone: 81(3)3219-5641 Fax. 81(3)3219-5710URL http://www.shimadzu.com

Founded in 1875, Shimadzu Corporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. We maintain a global network of sales, service, technical support and applications centers on six continents, and have established long-term relationships with a host of highly trained distributors located in over 100 countries. For information about Shimadzu, and to contact your local office, please visit our Web site at www.shimadzu.com

Achieving Greater Simpl ic i ty and Rel iabi l i ty in the Determinat ion of Amino Acid Sequences

Shimadzu Protein Sequencer

PPSQ-31A/33A

A c h i e v i n g G r e a t e r S i m p l i c i t y a n d R e l i a b i l i t y i n

t h e D e t e r m i n a t i o n o f A m i n o A c i d S e q u e n c e s

P P S Q - 3 1 A / 3 3 A

Analytical Stabil i ty

Features

P 04 - Analytical Stability

P 05 -

P 06 - Contents

Excel lent basel ine stabi l i ty and retent ion t ime reproducibi l i ty

Simple operat ions and easy-to-use data analysis funct ions

Repeated Use of Mobile Phase

Software with Excellent Operabil i ty

Reduced running costs and l iquid waste in the analysis of PTH-amino acids using isocrat ic mode

Software with Excellent Operability

Repeated Use of Mobile Phase

P 07 - Specifications

Achieving Greater Simpl ic i ty and Rel iabi l i ty in the Determinat ion of Amino Acid Sequences

Shimadzu Protein Sequencer

PPSQ-31A/33A

A c h i e v i n g G r e a t e r S i m p l i c i t y a n d R e l i a b i l i t y i n

t h e D e t e r m i n a t i o n o f A m i n o A c i d S e q u e n c e s

P P S Q - 3 1 A / 3 3 A

Analytical Stabil i ty

Features

P 04 - Analytical Stability

P 05 -

P 06 - Contents

Excel lent basel ine stabi l i ty and retent ion t ime reproducibi l i ty

Simple operat ions and easy-to-use data analysis funct ions

Repeated Use of Mobile Phase

Software with Excellent Operabil i ty

Reduced running costs and l iquid waste in the analysis of PTH-amino acids using isocrat ic mode

Software with Excellent Operability

Repeated Use of Mobile Phase

P 07 - Specifications

4

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min-0.25

0.00

0.25

0.50

0.75

1.00

1.25

1.50

1.75

2.00

mV

D

E

TH

N Q S

G AY R

M VP W

F

K

I L

PTH-AA

2D Electrophoresis Image of Proteins Extracted from E. Coli

10pmol

Spot 1

Sequence analysis can be started by simply registering the number of analysis cycles, data file names, and other information, and then clicking the [Start] button.

When a reagent is replaced, the inside of the reagent bottle is purged with nitrogen. The replacement dates and times of the reagents can be ascertained with a glance, and lot control is possible.

5

Stable Analysis, Low Running Cost, Excellent Operabil i ty

Analytical Stabil i ty

Baseline Stabil i ty

Repeated Use of Mobile Phase

PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.

Analysis of PTH-Amino Acids Using Isocratic ModePerforming PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.

Software with Excellent Operabil i ty

Simple Operations

Main Window

Sequence Schedule Window Reagent Control Window

Specialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.

Retention Time Reproducibil i ty

Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.)

Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.

cycle 1 cycle 2

cycle 14 cycle 15

cycle 24 cycle 25

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.0

0.2

0.4

0.6

0.8

1.0 mV

DTT

S

DMPTU DPTU

DPU DTT

T

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.0

0.2

0.4

0.6

0.8

1.0 mV

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

DMPTU

LDPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

Y

DTT DPTU

DMPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

EDTT

DMPTUDPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50mV

K

DTT

DMPTUDPTU

Selection of sequencing schedule

Summary display of currently executed cycle and step and estimated completion time

4

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min-0.25

0.00

0.25

0.50

0.75

1.00

1.25

1.50

1.75

2.00

mV

D

E

TH

N Q S

G AY R

M VP W

F

K

I L

PTH-AA

2D Electrophoresis Image of Proteins Extracted from E. Coli

10pmol

Spot 1

Sequence analysis can be started by simply registering the number of analysis cycles, data file names, and other information, and then clicking the [Start] button.

When a reagent is replaced, the inside of the reagent bottle is purged with nitrogen. The replacement dates and times of the reagents can be ascertained with a glance, and lot control is possible.

5

Stable Analysis, Low Running Cost, Excellent Operabil i ty

Analytical Stabil i ty

Baseline Stabil i ty

Repeated Use of Mobile Phase

PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.

Analysis of PTH-Amino Acids Using Isocratic ModePerforming PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.

Software with Excellent Operabil i ty

Simple Operations

Main Window

Sequence Schedule Window Reagent Control Window

Specialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.

Retention Time Reproducibil i ty

Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.)

Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.

cycle 1 cycle 2

cycle 14 cycle 15

cycle 24 cycle 25

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.0

0.2

0.4

0.6

0.8

1.0 mV

DTT

S

DMPTU DPTU

DPU DTT

T

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.0

0.2

0.4

0.6

0.8

1.0 mV

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

DMPTU

LDPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

Y

DTT DPTU

DMPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

EDTT

DMPTUDPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50mV

K

DTT

DMPTUDPTU

Selection of sequencing schedule

Summary display of currently executed cycle and step and estimated completion time

6 7

Software with Excellent Operabil i ty / Specif ications

Simple, Easy-to-Use Data Analysis FunctionsSpecif icationsSpecialized protein sequencer software makes it simple to perform the reprocessing of chromatograms, the

overlay of multiple chromatograms, and the automatic estimation of amino acid sequences, which are required for sequence analysis.

Reprocessing of chromatograms required

for data analysisOverlay of multiple

chromatograms

Automatic Sequence Estimation

Automatic estimation of amino acid sequences

Chromatogram peak integration, printing, and other processes can be performed on a per-sample basis.

Displays multiple chromatograms. Analysis cycles can be updated simply by clicking a button.Window allows easy identification of sequences.

Calibrates PTH-amino acids.Retention times can be edited simply by selecting a PTH-amino acid and clicking a peak.

Automatically generates a report containing analytical parameters, estimated sequences, yield rates, and other information.

If the appropriate settings are entered before analysis starts, sequences are automatically estimated after each cycle is complete. Up to four candidate amino acids are displayed together with their certainty levels.

Customized Repor ts

Reports containing information such as analytical parameters, estimated sequences, and yield rates are automatically created.

Yield Graph Display

Initial and repeated yield rates are calculated and displayed in graph form. Amino acids used for calculations can be freely selected.

Reaction method

Reaction time

Number of reactors

Sample immobilization method

Reactor temperature control

Converter temperature control

Column temperature control

Number of reagents/solvents

Reagent/solvent delivery method

Dimensions

Weight

Edman degradation

PPSQ-31A: 47 min/cycle

PPSQ-33A: 48.5 min/cycle

PPSQ-31A: 1

PPSQ-33A: 3

Glass fiber disk (8 mm dia.) or PVDF membrane

{Room temperature + 10 °C} to 60 °C

{Room temperature + 10 °C} to 70 °C

{Room temperature + 10 °C} to 60 °C

8 (one is an option)

Nitrogen gas pressure

650 (W) x 520 (H) x 500 (D) mm

PPSQ-31A: 55 kg

Main Unit

Reagents for amino acid sequence analysis (7 types)

Glass fiber disks

Polybrene reagent

Apomyoglobin standard

Mobile phase for PTH-amino acids

Separation column for PTH-amino acids

Mixed standards for PTH-amino acids

1 of each type

50

1

1

1 L

1

2 mL x 4

Part name QuantityPPSQ-31A/33A

Installation space

Power supply

Nitrogen gas

Exhaust equipment

Desktop: 1,730 (W) x 600 (D) x 550 (H) mm min.

(Weight: Approx. 93 kg)

100 VAC, 1,100 VA, 50/60 Hz

Purity: 99.9999% min.

A cylinder pressure regulator and gas tubing

(10 m) are provided as standard accessories.

In order to provide an exhaust for gases

produced by waste liquids, an exhaust tube

must either be connected to exhaust

equipment or led outside. An exhaust tube

(20 m) is provided as a standard accessory.

Flow rate setting range

Dimensions

Weight

0.001 to 10.000 mL/min

260 (W) x 140 (H) x 420 (D) mm

11 kg

Solvent Delivery Module LC-20AT

292-31310-XX

292-05684-92

PPSQ-31A (main unit)

LC-20AT

SPD-20A

PPSQ-30 (control PC set)

Startup kit

Part number Part name

Wavelength setting range

Dimensions

Weight

190 to 700 nm

260 (W) x 140 (H) x 420 (D) mm

13 kg

Detector SPD-20A

Computer

Display

Note: In the interests of product improvement, these specifications may change without notice.Note: Microsoft is a registered trademark of Microsoft Corporation in the U.S. and other countries.Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.

Note: The startup kit is for initial installation. To order additional reagents, contact Wako Pure Chemical Industries, Ltd.

Note: The required installation space and power supply may change according to the PC and display used.

Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.

Offers highly cost-effective performance.

PPSQ-33A Triple Reactor (Supports consecutive analysis of 3 samples.)

IBM PC/AT or compatible

OS: Microsoft® Windows® XP

17-inch LCD

Control PC

Standard Configurations

Star tup Kit

Other I tems Provided by User

PPSQ-31A System

292-31330-XX

292-05684-92

PPSQ-33A (main unit)

LC-20AT

SPD-20A

PPSQ-30 (control PC set)

Startup kit

Part number Part name

PPSQ-33A System

PPSQ-31A Single Reactor

PPSQ-31A/33A

ShimadzuProtein Sequencer

C297-E055A

Printed in Japan 3295-12801-10ANS

The contents of this brochure are subject to change without notice.

JQA-0376

SHIMADZU CORPORATION. International Marketing Division3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan Phone: 81(3)3219-5641 Fax. 81(3)3219-5710URL http://www.shimadzu.com

Founded in 1875, Shimadzu Corporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. We maintain a global network of sales, service, technical support and applications centers on six continents, and have established long-term relationships with a host of highly trained distributors located in over 100 countries. For information about Shimadzu, and to contact your local office, please visit our Web site at www.shimadzu.com