Role of the Cytoplasmic Domain in Anabaena Sensory Rhodopsin

Photocycling: Vectoriality of Schiff Base Deprotonation

Sineshchekov, O. A.; Spudich, E. N.; Trivedi, V. D.; and Spudich, J.L.

Biophysical Journal, 91, 4519-4527, 2006

Introduction

• Rhodopsins• Retinal linked to

opsin in Schiff base linkage to lysine residue

Bacteriorhodopsin• With hv, all-trans to cis- retinal• H+ transfer from Lys216 to Asp85• Glu204 and Glu194 transfer H+ to

EC side• Asp 96 reprotonates Lys216• SRII:

Bacteriorhodopsin

Bacteriorhodopsin