siPOOLTM

Human Kinase LibraryRNAi Screening Results You Can Trust

Problems faced with single siRNA/pools of 3-4 siRNAs

Off-target spiking experiment demon-strates high complexity pools > 15 siRNAs required to dilute off-target effects.

Off-target effects that give rise to variable, mixed phenotypes

For the first �me, medium to high-throughput RNAi screening with siPOOLs is enabled with the siPOOL human kinase library.

Key Benefits

Reliable RNAi screening results

The excep�onal targe�ng specificity and efficient gene silencing by siPOOLs: • reduces false posi�ves and improves detec�on of true hits • eases data analysis • improves consistency between screens

Save �me and resources • Simplify screening with one siPOOL per gene • Obtain results quickly (easily applied across cell lines with results in days) • Avoid inefficient tes�ng of mul�ple siRNAs to confirm hits

Variable results between siRNAs limit data interpreta�on

Falkenberg et al. show that siRNA results are widely divergent upon deconvolution of siGENOME smartpools.

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Read

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6

8

5 10 15 20

mini-pool (pass 1)deconvoluted siRNA (pass 2)

0

10

20

30

40

50

60

70

80

90

100

PIM

1TR

IB1

NEK

8CL

K2TN

IKAB

L2AD

CK1

ULK

3AM

HR2

DA

PK3

CLK4

TAO

K2CA

MK2

GPI

M3

BRD

2-p2

1M

AP3

K9CD

K6-p

21M

AP3

K13

PAN

3SI

K1ST

K17A

STK1

7BG

SG2

SGK2

MA

P3K1

0ER

BB4

STK2

5EP

HA1

SIK3

WEE

1CD

K16

MTO

RRA

F1SI

K2CD

K2EG

FRPI

M2

FGFR

3RI

OK1

STK1

6-p2

1CS

NK1

G3

LATS

2FG

FR4

PRKA

A1AK

T1CH

EK1

CDC7

PRKC

HTA

OK1

MA

RK2

SRPK

1ER

BB3

PAK1

PIK3

C2A

CHEK

2SR

PK2

KSR1

ARA F

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MET

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C3G

SK3A

DD

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AP2

K5AB

L1M

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1TA

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P4K4

STK3

PIK3

CA CSK

PLK1

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TGFB

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PDG

FRB

IRAK

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MYL

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siPOOL Human Kinases (83 siPOOLs, 1-3 nM)

70 % KD

Data with the siPOOL Human Kinase Library

Reproducible phenotypes

siPOOLs efficiently counter siRNA variability. Two siPOOLs against the same gene (36 tested) produced more reproducible phenotypes than single siRNAs.

Efficient gene silencing at low nanomolar concentra�ons

Efficient at very low concentra�ons, siPOOLs can be mul�plexed with other treatments or siPOOLs without risk of side-effects (applicable for synthe�c lethality screens).

Majority of human kinase siPOOLs tested (71 of 83) produced ≥ 70% gene knockdown at 1-3 nM in standard cell lines (A549, MCF7, HeLa) as measured by rt-qPCR.

Email info@sitools.dePhone +49 89 12501 4800 Fax +49 89 8955 7281Address LochhamerStr. 29A

82152 Mar�nsried/PlaneggGermany

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Ordering informa�on

• Provided in 96/384-well plates or 2D barcoded tubes • Available lyophilized or in solu�on • 0.1 – 1 nmol scales • Plate arraying service available • Custom extensions possible

Please contact us or our distributors for pricing(visit About > Distributors on our website).

References:Falkenberg, K. J., Gould, C. M., Johnstone, R. W. & Simpson, K. J. Genome-wide functional genomic and transcriptomic analyses for genes regulating sensitivity to vorinostat. Sci. Data 2014 1 1, 140017 (2014).

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