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CONTENTSPrefaceOverviewAbbreviations and Acronyms1. Transcriptional Regulation and Chromatin
Structure and Function in Eukaryotic Cells2. Initial Strategic Issues3. Transcription Initiation Site Mapping4. Functional Assays for Promoter Analysis5. Identification and Analysis of Distant
Control Regions
6. Identifying cis-Acting DNA Elements withina Control Region
7. Identification of DNA-Binding Proteins andTheir Genes
8. Confirming the Functional Importance of aProtein–DNA Interaction
9. Analysis of Transcription Factor–ChromatinInteractions on Endogenous Control Region
10. Identifying and Characterizing Domains ofTranscription Regulatory Factors
11. DNA Binding by Regulatory TranscriptionFactors
12. Transcription and Preinitiation ComplexAssembly In Vitro
13. Studying Chromatin Dynamics In Vitro:Chromatin Assembly, Remodeling, andTranscription
IndexAppendix: Cautions
By Michael F. Carey, University of California, Los Angeles; Craig L. Peterson, University ofMassachusetts Medical School, Worcester; and Stephen T. Smale, University of California, Los Angeles
Strategies for studying gene regulation mechanisms have changed dramatically over the past several years in light of the emergence of complete genome sequences for many
organisms as well as the development of or improvements to technologies such as chromatinimmunoprecipitation, RNA interference, microarrays, and proteomics.The first edition of the highly successful Transcriptional Regulation in Eukaryotes, written by Michael Carey and StephenSmale at UCLA, provided a comprehensive source of strategic, conceptual, and technical information for investigating thecomplexities of gene regulation at the level of transcription.With the ever-increasing importance of genome data and the appearance of new and better techniques, the second editionof this textbook has added a third author, Craig Peterson at the University of Massachusetts Medical School. In additionto a new chapter on the in vitro analysis of chromatin templates for DNA-binding studies and transcription, this secondedition has been extensively rewritten and updated to discuss new advances in the field and their impact on gene regulation mechanisms. The second edition retains the approach of the first in covering both the conceptual and practicalaspects of how to study the regulation of a newly isolated gene and the biochemistry of a new transcription factor.Transcriptional Regulation in Eukaryotes is a powerful textbook and manual for advanced instruction in molecular biology which
• supplements clearly written text with extensive illustrations• puts methods in the context of underlying theory• gives expert recommendations on experimental strategies• encourages creativity in investigative design• explains protocols for essential techniques step by step, with extensive advice on troubleshooting• provides the latest methods in use in the field
This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systemsor disease states, providing underlying theory and perspective to the newcomer and the latest techniques to the expert.
Due December 2008, 640 pp. (approx.), index, appendixHardcover $240 ISBN 978-087969777-8Paperback $165 ISBN 978-087969762-4
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