| Date post: | 14-Aug-2015 |
| Category: |
Technology |
| Upload: | redi-aditama |
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Bioteknologi di Wilmar: Peranan Teknologi Enzim dan Bioinforma8ka dalam Industri Berbasis Kelapa Sawit yang Berkelanjutan
Redi Aditama, M.Si R&D Biotechnology!PT. Wilmar Benih Indonesia
Who We Are?!Wilmar International!
Who We Are?!Wilmar International >>> Overview!
Who We Are?!Wilmar International >>> Business Segment!
Who We Are?!Wilmar International >>> Global Reach!
Why Oil Palm?!Elaeis guineensis!
Oil Palm: Elaeis guineensis
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Why Oil Palm? !Importance of Palm Oil!
Why Oil Palm?!Palm Oil Price!
Why Oil Palm?!Palm Oil Producers, Importers and Consumers!
Why Oil Palm?!Oil Palm Productivity!
Biotechnology!Overview!
Biotechnology!Oil Palm Industry!
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Biotechnology!Oil Palm Plantation!
Biotechnology!Oil Palm Plantation!
• DNA Sequencing • Gene8c Engineering • Molecular Breeding
• Tissue Culture • Gene Transforma8on
• Bio-‐fer8lizer • Growth Promo8ng
Bacteria • Growth Promo8ng
Compound
• An8fungal Compound • An8fungal Bacteria • Pest Control • Bio-‐pes8cide
Biotechnology!Oil Palm Plantation >>> Molecular Biotechnology!
DNA Sequencing • Sanger
Sequencing • Next
Genera8on Sequencing
Molecular Breeding • Marker assisted
breeding • Gene8c
Engineering
Biotechnology!Oil Palm Plantation >>> Tissue Culture!
Advantages:!• Exact copy!• Quick production!• Regeneration of
genetically modified plants!
• Free of diseases, pests and pathogens!
Biotechnology!Oil Palm Plantation >>> Antifungal, Pest Control!
Biotechnology!Palm Oil Processing!
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Biotechnology!Palm Oil Processing!
Biotechnology!Palm Oil Processing >>> Bioenergy!
Biodiesel Plant!
Biorefinery Plant! Enzymatic Biodiesel Pilot Plant!
Enzyme Development!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Biorefinery!
• Convert Refined Palm Oil to Olefin and specialty mid chain triglycerides
• Joint Venture Plant Between Wilmar Interna8onal Ltd and Elevance Renewable Science Inc.
• Process based on the Nobel Prize-‐Winning olefin metathesis catalyst technology developed by Professor Robert H. Grubbs
Biorefinery!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Biorefinery!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Biodiesel Plant!
• 3 Plants X 1000 MT / Day • All 3 Plants based on CD Process for trans-‐
esterifica8on
• Main Raw Materials: • Refined Oils • Methanol • Sodium Methylate • Caus8c Soda • Potassium Methylate
• Products • Biodiesel / Methyl Ester • Crude Glycerin • Fa^y Ma^er Biodiesel!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Biodiesel!
• Methyl ester of vegetable oil • Renewable source • Less pollu8ng than petroleum
diesel
• Can be used in exis8ng diesel engine
• Have lubrica8ng property,
lengthen the life8me of engines
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Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzymatic Biodiesel!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzymatic Biodiesel!
Base catalyzed biodiesel synthesis 1. High cost of wastewater
treatment 2. Produce FFA in the presence of
water 3. Need addi8onal purifica8on to
remove catalyst
Enzyma8c biodiesel synthesis: 1. Low cost wastewater
treatment (environmentally friendly)
2. Convert FFA to methyl ester 3. Just need simple separa8on
to remove catalyst
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development!
Screening Recombina8on Modifica8on Immobiliza8on Applica8on
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development >>> Screening!
Environmental Microorganism! Exotic Plants!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development >>> Molecular Cloning!
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Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development >>> Modification!
T ! != 20-30 °C!pH ! != ~7!Solvent != water!
T ! != > 50 °C!pH ! != < 7!Solvent != methanol!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development >>> Protein Engineering!
2. Structure <-‐-‐-‐> Func8on
1 .
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development >>> Protein Engineering!
Enzyme WT
Sequencing
DNA Sequence
Transla8on
PepCde Sequence
Modeling
Protein Structure
In silico muta8on
Mutant Structure Mutant Sequence
Mutagenesis
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development >>> Enzyme Immobilization!
Biotechnology!Palm Oil Processing >>> Bioenergy >>> Enzyme Development >>> Application!
Bioinformatics!Palm Oil Company!
Assembly! Alignment! BLAST! Phylogenetic!
Metagenomic! Structure Prediction! Protein Modeling! Molecular Dynamics!
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Sustainable Company!Wilmar Group!
Criteria for Sustainable Business 1. It incorporates principles of
sustainability into each of its business decisions.
2. It supplies environmentally friendly products or services that replaces demand for nongreen products and/or services.
3. It is greener than tradi8onal compe88on.
4. It has made an enduring commitment to environmental principles in its business opera8ons.
Biotechnology
Thank You
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