Slow-Release and Non-Release Antimicrobial Coatings with ...

Slow-Release and Non-Release Antimicrobial

Coatings with Enhanced Protection

Presenter: Amir Kazemi

2021 TriMiS Inc. - Confidential 2

Introduction

© 2021 TriMiS Inc. All rights reserved

2021 TriMiS Inc. - Confidential

Our goal is to offer advanced paints and coatings customized to

eliminate specific microorganisms (required by application)

while meeting environmental regulations, durability and service

life targets.

© 2021 TriMiS Inc. All rights reserved3


Our Story: Mold Issue in Meat and Dairy Industry

Environmental conditions are favorable for

mold generation on different surfaces.

Presence of mold can cause numerous

problems including health and food safety

concerns.© 2021 TriMiS Inc. All rights reserved4


Other Common Areas of Problem



● Annual spending on mold remedy exceeds multi $Bs.

● The average cost of mold remediation ranges from $2,000 to $6,000.

● HVAC or Air Duct Mold Treatment can cost up to $10,000 since ventilation

systems require special cleaning.

Mold Issue: Some Key Numbers in Constructions



Construction

Agriculture

Healthcare

Farm & Barn

Target Markets and Applications

White Roofing

Food Industry

Antimicrobial (mold & bacteria)

and Antiviral coating and paint

products for several commercial

and industrial applications.

Commercial

Sports Arena


2021 TriMiS Inc. - Confidential © 2021 TriMiS Inc. All rights reserved

Antimicrobial Products

Slow-release

Anti-moldConstruction (e.g.

roofing, basements, drywalls, circulation

system, etc.)

Food industry (e.g. Dairy farms, animal

barns, food processing, etc.)

Anti-algae

Anti-bacterial

Non-release

Anti-viral

Anti-bacterial

‘High-touch’ surfaces and non-medical

applications

• Customized, target-sensitive and application-based coatings using different additives.

Our Platform

8


Antimicrobial (Antimold) Paint

Slow-release Technology



1

2

0

3

4Water based solution with

low or zero Volatile Organic

Compounds (VOC) content.

Protection against a wide

range of microorganisms

such as molds, bacteria,

fungi and viruses.

Acrylic resin, hence water

resistant, high tear

strength, flexible, good

adhesion, and high

durability.

Embeds biocides and

enables very efficient slow

release system to meet

customer specs.

Rapid customization

for high efficacy

Safer Coating

Durable and

Applicable to any

Surfaces

3

Targets a wide

variety of

Microorganisms

Our Solution



The biocide, resin

and crosslinker (type

and concentration)

are carefully selected

for each application.

Biocide is released

slowly over the

service life.

Either no antimicrobial

agents used or

antimicrobial agents

simply mixed with the

paint at a certain

concentration.

Slow-release vs Conventional Products


US Patent filed 63/084,429; Sep 2020.

Slow-release

11


Slow-release Coating Technology

• The controlled-release mechanism:

• improves the antimicrobial efficiency of the coating

• minimizes the environmental impacts.

• Biocides are incorporated into the paint formulation such that they are slowly

released under certain environmental conditions.


US Patent filed 63/084,429; Sep 2020.

12


• Coating samples with different formulations were tested for their anti-mold and anti-algal

efficiency:

• Mold/Mildew resistance tests were performed with standard ASTM D5590

organisms (Aureobasidium pullulans, Aspergillus niger, Penicillium

funiculosum) as well as Aspergillus flavus (ATCC #9643).

• Algae resistance tests were performed with standard ASTM D5589

organisms (mixed algae culture).

• The dry film samples were tested with and without 48 hours of leaching before

testing for 6 different formulations mixed with 3 types of biocides (18

combinations and 4 controls).

Resistance Tests (Mold & Algae) - Results



Zone of Inhibition

(c)

• The Zone of inhibition is a circular area around the spot of an antimicrobial agent in

which the microorganism does not grow.

• The zone of inhibition can be used to measure the susceptibility of a microorganism

towards an antimicrobial agent.





Legend

15


● 6 Formulations

(different types and

percentages of resins

and crosslinkers)

● 3 Types of Biocides




● 6 Formulations



and crosslinkers)







Conventional

Acrylic Paint

(Almost no

antimicrobial

activity)

18




Strong

antialgae

activity

19


A Comparison

between z-values

• A higher z-value

shows higher

antimicrobial activity,

but it could be an

indication of the speed

of leaching of the

antimicrobial agents

as well.

• Lower and more

stable z-values are

preferred for some

applications.© 2021 TriMiS Inc. All rights reserved


20


• Results plotted based on Z-value for each microorganism/resin combination

• Z-value variation between 0 and 48 h leaching and average were calculated



0

2

4

6

8

10

12

14

16

33 50 66

Zon

e of

Inh

ibit

ion

(m

m)

Resin (%)

A. flavus (X)

Biocide 1

Biocide 1-48h

Biocide 2

Biocide 2-48h

Biocide 3

Biocide 3-48h

0

2

4

6

8

10

12

14

16

0 5 15

Zon

e of

Inh

ibit

ion

(m

m)

Crosslinker (%)

A. flavus (R)

Biocide 1

Biocide 1-48 h

Biocide 2

Biocide 2-48h

Biocide 3

Biocide 3-48h

21






0

4

8

12

16

20

33 50 66

Zon

e of

Inh

ibit

ion

(m

m)

Resin (%)

A. pullulans (X)

Biocide 1

Biocide 1-48h

Biocide 2

Biocide 2-48h

Biocide 3

Biocide 3-48h

0

4

8

12

16

20

0 5 15

Zon

e of

Inh

ibit

ion

(m

m)

Crosslinker (%)

A. pullulans (R)

Biocide 1

Biocide 1-48h

Biocide 2

Biocide 2-48h

Biocide 3

Biocide 3-48h

22






0

2

4

6

8

10

12

33 50 66

Zon

e of

Inh

ibit

ion

(m

m)

Resin (%)

A. niger

P. funiculosum

(X)

Biocide 1

Biocide 1-48h

Biocide 2

Biocide 2-48h

Biocide 3

Biocide 3-48h

0

2

4

6

8

10

12

0 5 15

Zon

e of

Inh

ibit

ion

(m

m)

Crosslinker (%)

A. niger

P. funiculosum

(R)

Biocide 1

Biocide 1-48h

Biocide 2

Biocide 2-48h

Biocide 3

Biocide 3-48h

23


● 6 Formulations



and crosslinkers)




X2

X3

Average Variation: 1.33 mm


Biocide 2 is VOC free

24


● 6 Formulations



and crosslinkers)




X2

X3



Biocide 2 is VOC free

X2

R1



If algal protection is needed.

25


Weathering Test Results












Microorganism Portfolio

Microorganisms Mold/Mildew Bacteria Algae

Existing

A. niger,

P. funiculosum

A. pullulans

A. flavus

G. magma

(cyanobacteria,

known as dark

green algae)

E.Coli

Salmonella

Algal Mixture

In Progress/

Future Work A. parasiticus

P. aeruginosa

C. perfringens

E. Tenella



Antiviral Coating

Technology



QAC

Silane Group

• Silane-based formulation:

• Conventional disinfection methods are tedious, laborious and not durable.

• ‘High-touch’ surfaces in public spaces need to be disinfected repeatedly.

Adhesion

promoter (silane

coupling agent)

Non-Release Antiviral Coating



● Formulation was developed based on a non-release approach using quaternary

ammonium compounds, silane-based adhesion promoters, and other additives as

applicable.



2021 TriMiS Inc. - Confidential © 2021 TriMiS Inc. All rights reserved


Antiviral groups (1601, and 1706–1730 cm−1).

AP and surface attachment groups (915, 1090, and 2840-2850 cm−1).

Tra

nsm

itta

nce

34


Antiviral Coating for High Touch Surface

❑ The Coating was tested according to ISO 21702 method (measurement of antiviralactivity on plastics and other non-porous surfaces). The test consists of 3 steps:

1. Cytotoxicity Testing: The purpose of this test is to determine if the test specimenscause cytotoxicity to the cell line used for measuring virus infectivity.✅

2. Verification of Cell Sensitivity to Virus: The purpose of this test is to confirm thatneither the test specimen materials nor the treatment alters the cell sensitivity toviral infection.✅

3. Antiviral Activity Testing: Three treated and six untreated specimens are tested todetermine the effectivity of the coating against the HCoV-229E after 2 hours.🔜

35


Antiviral Coating for High Touch Surface - Cytotoxicity

• A value of 1 indicates

there is no change in

cell viability compared

to the untreated

controls.

• A value less than 1,

indicates that cell

viability was decreased,

suggesting potential

cytotoxic effects.

• Treatment A had no

cytotoxicity.

36


Antiviral Coating for High Touch Surface - Verification

of Cell Sensitivity to Virus

▪ Both the treated and untreated washing out solutions had the same infectivity as

the control. Therefore, there are no assay interferences from the sample. Based on

these results, it was determined to proceed to antiviral testing.

▪ Step 3 (antiviral testing) is in progress.

37


Conclusions and Recommendations

• Different formulations of the developed antimold coating showed significant

antifungal, and in some cases, antialgae and antibacterial activity.

• Changes were observed on a case-to-case basis, but in most cases, there is a

slight decrease in Z-value after 48 h of leaching and when using a higher resin

concentration.

• It is recommended that a formulation with a lower z-value and z-value variation is

used (i.e. biocide leaching is not significant).

• A clear antiviral coating that was formulated based on a non-release mechanism

showed promising results against the HCoV-229E.

• Further work is required to investigate the improve the mechanistic

understanding of the slow-release and to characterize the non-release coating

(surface charge quantification)



Partners and Collaborates


Contact Us:

[email protected]

[email protected]

[email protected]

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mailto:[email protected]

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