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Smart strips - Tecna rapid tests for mycotoxins

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The rapid and cost effective screening solution for multiple mycotoxins screening
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Page 1: Smart strips - Tecna rapid tests for mycotoxins

The rapid and cost effective screening solution for multiple mycotoxins screening

Page 2: Smart strips - Tecna rapid tests for mycotoxins

Mycotoxins co-occurrence

Almost 70% of the cereals in the world present mycotoxin contamination

In 30% of the cases the contamination levels are well above the law limits

With all these premises, mycotoxins co-occurrence is a widely diffused phenomenon

Different toxic fungi proliferation can be favoured by similar climate conditions

° C 15 20 25 30 35

ZON, T2

DON

DON

FUMONISINS

AFLATOXINS

Aspergillus flavusAspergillus parasiticus

Fusarium moniliformeFusarium verticilloides

Fusarium proliferatum

Fusarium graminearum

Mycotoxins are widely diffused toxic metabolites of different fungi species

Page 3: Smart strips - Tecna rapid tests for mycotoxins

Mycotoxins toxicity main targets are:

- Liver- Kidneys- Immune system- Nervous system- Endocryne system- Mucosa- Reprpductive

system

The effects of mycotoxins contamination on human and animal health can be predictable in case of single mycotoxin occurrence. However, if there are several occurring mycotoxins, the relative synergistics effects are barely known and can be unpredictable.

Mycotoxins co-occurrence

Noteworthy, in case of multiple mycotoxins contamination, adverse effects might occur even if the single mycotoxins levels are lower than regulatory ones.

Page 4: Smart strips - Tecna rapid tests for mycotoxins

The field

Theharvest

Thestocking

The transit

The acceptance

check

The processing

There are some critical points when SPEED and EASE of USE of screening tests are fundamental.

Possibility of parallel screening is the key factor to rapidly determine mycotoxin co-occurrence and to asses products safety.

Mycotoxins not only develop in the field; they proliferate along the whole agro-food chain as well.

Screening along the agro-food chain

SPEED and EASE of USE of a test not necessarily enable to avoid the risks of mycotoxins co-occurrence.

Page 5: Smart strips - Tecna rapid tests for mycotoxins

Deoxynivalenol

Aflatoxins

Zearalenone

Fumonisins

Single screeningneeds

- 4 exctracts to prepare- 4 reading sessions- 4 time consumables usage

Parallel screeningneeds

- Single exctract to prepare- One reading session- Use consumables just

once

Parallel screening provides unravelled time and money save on mycotoxins screening.

Tecna Smart Strips for mycotoxins enable parallel screening for the main mycotoxins as aflatoxins, deoxynivalenol, zearalenone and fumonisins.

Parallel screening not only provides resources save but also reduces error probability as less steps are performed.

Single screening vs parallel screening

Page 6: Smart strips - Tecna rapid tests for mycotoxins

Tecna Smart Strip kits share the same sample preparation and assay procedure:

1. Weight the ground cereal2. Add the extracting solution3. Shake 3 minute4. Filter5. Dilute

6. Mark the cassette 7. Add the extract on the strip8. Wait 5 or 10 minutes – incubation at ROOM TEMPERATURE

9. Read

Tecna Smart Strip - general features

Page 7: Smart strips - Tecna rapid tests for mycotoxins

Tecna Smart Strip – steps are illustrated in the Smart Chart

Every kit contains an illustrated Smart Chart to support analysts in performing the assay

Page 8: Smart strips - Tecna rapid tests for mycotoxins

The LAB LFD READER allows to analyze up to 5 strips per time, even for different analytes. The provided result is either qualitative or quantitative.

Key features of the LAB LFD reader are:• little space required• PC connession through USB wire• read out of max 5 strips per time – different tests also• software for results analysis• software to archive results

Tecna LAB FLD READER for digital analysis of results

Page 9: Smart strips - Tecna rapid tests for mycotoxins

MAIZE

WHEAT

Samplepreparation

DURUM WHEAT

1:3 in 70% methanol extraction, shake for 3 minutes, filter or centrifuge, dilute

1:5 in water extraction,

shake for 3', centrifuge,

dilute

WHEAT

Tecna Smart Strip - technical features

2 – 75 ppbUp to 10 – 375

ppb

Detection range

50 – 1000 ppbUp to 100 – 2000

ppb

150 – 4000 ppbUp to 750 – 20000

ppb

LOQ 5 ppb 75 ppb 250 ppb 500 ppb

Assay time 10 min 10 min5 min

10 ppb 100 ppb

Assay time 5 min

250 -12500 ppb

Applicability

Cut off

Quantitative analysis

Qualitative analysis

Page 10: Smart strips - Tecna rapid tests for mycotoxins

Aflatoxin B1 and zearalenone Smart Strips allow a qualitative pre-reading of the result within specific cut offs.

Quantitative results are obtained for aflatoxin B1, zearalenone, fumonisins and deoxynivalenol.

Batch specific barcode is the easy way to set the LAB LFD READER for the ananlysis for one or more mycotoxins simultaneosly.I.e: - analysis for one mycotoxin = 1 barcode

- analysis for three mycotoxins = 3 barcodes

Tecna Smart Strip - technical features

The plastic house of the Smart Strip can be used for labelling thereof, thus favouring sample tracking during multiple screening for mycotoxins.

Page 11: Smart strips - Tecna rapid tests for mycotoxins

Smart Strip AFLA B1

20 det. – cod. MA820

Smart Strip ZON 20 det. – cod. MZ800

Smart Strip FUMO 20 det. – cod. MF800

Smart Strip DON 20 det. – cod. MD800

For more information on the rapid test line dedicated to mycotoxins screening

[email protected]

www.tecnalab.com

For further information on mycotoxins look for the Tecna Mycotoxins Cards in all ELISA and lateral flow kits or directly book your own deck. Tecna

Mycotoxins Cards: learning about mycotoxins has never been that easy!

Tecna Smart Strip - the product lineup


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