Some hematological and biochemical parameters of Arsi Bale sheep naturally infected with Facilola sp.

Sandip Banerjee, Kefelew B and Sintayehu YDepartment of Animal and Range Science

Hawassa University

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Arsi Bale sheep is the most predominant sheep in Hawassa

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Liver fluke is one of the important causes of loss in carcass weight

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Damage to liver causes loss in the value of carcass, besides death in many cases

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Most of the times the whole organ is to be discarded

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Different stages of infection

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

The serum biochemical traits were analyzed using Humastar-300

Serum alkaline phosphatase (ALKP), Serum aspartate aminotransferase (AST),

Serum aniline amino transferase, (ALT)Serum cholesterol( CH),

Serum creatinine (CREA), Serum total protein (TP)

and Serum triglycerides (TRI)

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Hematological traits were analyzed using Abbott CELL-DYN 1800

White blood corpuscles (WBC), Red blood corpuscles (RBC),

Lymphocytes (LYM),Total cell count (MID), Granulocytes, (GRAN) ,Hemoglobin ( Hb),

Hematocrit (HCT),Mean corpuscular volume (MCV), Mean corpuscular hemoglobin (MCH)

and Mean corpuscular hemoglobin concentration (MCHC).

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Parameters Infected Ram Non infected Ram Infected Ewe Non infected Ewe

Mean± SDRange

Mean± SDRange

Mean± SDRange

Mean± SDRange

ALKP٭٭(IU/L)

423.0 ± 134.4ª(254-622)

170.5± 17.6b

(158-183)467.3±205.17 ª

(285-917)163.5±22.35b

(136-202)ALT٭(IU/L)

23.6±5.4 ª(16-32)

15.5± 4.94 b

(12-19)25.0±15.8 ª

(10-61)17.33±6.7 b

(10-25)AST٭٭(IU/L)

292.4±172.25 c

(107-535)118.0± 4.24 ª

(115-121)232.0±179.9 b c

(83-682)165.83±37.8 ª b

(97-210)CH٭٭(mg/dl)

59.2±13.9bc

(38-79)37.0± 11.3 ª

(29-45)68.0±17.4c

(42-97)44.16±8.32 ªb

(34-55)CREA٭(mg/dl)

.75±.36 ª(.2-1.4)

.85±.07 ª(.8-.9)

.84±.35 ª(.2-1.3)

1.03±.1 b

(.9-1.2)TP٭(g/dl)

6.10±.79 ª(5.1-7.4)

5.1± 1.13 b

(4.3-5.9)5.8±1.11 ª

(4-7.5)5.23±1.32 b

(3.6-7.2)TRI٭(mg/dl)

20.11±6.0 ª(9-27)

15.5± 3.53 b

(13-18)21.2±10.76 ª

(4-43)15.16±4.5 b

(9-21)

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Results for Serum Biochemical traits

Parameters IInfected Ram Non infected Ram Infected Ewe Non infected Ewe

Mean± SDRange

Mean± SDRange

Mean± SDRange

Mean± SDRange

WBC٭(k/µl) 30.10±13.2 ª

(16-46.8)42.95± 12.51 b

(34.1-54.8)39.8±16.7 b

(17-67.8)44.21±16.09 b

(31.5-73)

LYM٭(RM) 26.93±12.7 ª

(13.5-42.8)38.5± 9.9 b

(31.5-41.5)36.6±15.5 b

(16.4-62.7)38.5±16.11 b

(25.5-65.6)

MID٭(RM) 2.34±1.07 ª

(.6-4.0)2.95±1.62 b

(1.8-4.1)2.2±1.02 ª

(.8-3.5)3.71±1.88 c

(1.8-6.3)

GRAN٭(RM) .811±.61 ª

(.2-2.0)1.4±1.13 b

(.6-2.2).94±.63 ª(.1-1.7)

2.0±1.52 c

(.6-4.8)

RBC٭(M/ µl) 4.78±1.01 ª

(3.43-6.31)5.65±.35 b

(5.4-5.9)5.34±1.27 b

(2.85-6.4)5.49±.76 b

(4.05-6.24)

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Results for some hematological traits-1

Parameters IInfected Ram Non infected Ram Infected Ewe Non infected Ewe

Mean± SDRange

Mean± SDRange

Mean± SDRange

Mean± SDRange

HB٭(g/dl)

9.87±2.63 ª(4.4-11.9)

11.05± 1.34 ª(10.1-12)

10.36±2.11 ª(6-13.1)

12.36±3.8 b

(8.7-18.2)

HCT٭(%) 16.32±2.8 ª

(13-20.8)18.15±1.35 b

(17.2-19.1)17.73±4.6 ªb

(8.7-22.2)18.23±2.84 b

(12.7-20.5)

MCV٭(fl)

34.64±2.62 ª(31.8-40.6)

32.15± .353 b

(31.9-32.4)32.64±1.006 b

(30.4-33.9)32.51±.82 b

(31.4-33.5)

MCH٭(pg)

15.78±3.3 ª(12-21.3)

19.5± 1.13 b

(18.7-20.3)19.32±3.7 b

(15.7-28.4)18.15±2.3 b

(15.7-20.5)

MCHC٭(g/dl)

50.11±10.4 ª(31.9-67.1)

60.75±2.9 b

(58.7-62.8)59.52±13.46 b

(47.7-93.1)56.6±8.21 b

(47.3-64.5)

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Results for some hematological traits-2

Results and Conclusion

Significant differences (P≤0.05)were observed in Serum Alkaline phosphatase ( ALKP) , serum aspartate aminotransferase (AST) and cholesterol( CH) levels of the infected and non infected sheep.There were differences in values of all the other parameters, both hematological and serum biochemical parameters, though not significant.It can thus be concluded that ALKP, AST and CH levels can indicate tehe extent of hepatic damage due to Faciola sp. in sheep

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.

Thank you

The 5th

All Africa Conference on Animal Agriculture Commercialization of Livestock Agriculture in Africa: Challenges and opportunities October 25-28, 2010.