Soy bean using iplant resources

How we started

• iPlant workshop –CSHL

Introduction

• 14 different samples – Seeds • 5, 6 and 7 stages well watered and drought stress

– Different parts of Roots• And 2 time points 72 and 96 hours as well well watered

and drought stress

Analysis-Quality checks

• We performed quality checks using FASTX toolkit – Locally as well as in DE

Analysis

• Tuxedo suite of tools• Bowtie for the alignment of the filtered data• Cufflinks suite for the analysis• We are checking the needs of the project so

that we can add more functionalities into our pipelines

Quality Analysis

SWS-R5 SWS-R6 SWS-R7 SWW-R5 SWW-R6 SWW-R7

Seeds

96-LRS-R2

Lateral Root

96-LRS-R1 96-LRC-R2 96-LRC-R1 72-LRS-R2 72-LRS-R1 72-LRC-R2 72-LRC-R1

GC contentSWS-R5 SWS-R6 SWS-R7 SWW-R5 SWW-R6 SWW-R7

Seeds

96-LRS-R2

Lateral Root

96-LRS-R1 96-LRC-R2 96-LRC-R1 72-LRS-R2 72-LRS-R1 72-LRC-R2 72-LRC-R1