Spinal Cord Injury

Topics in Bioengineering

http://neuroscience.uth.tmc.edu/s2/chapter03.html

Characterization of the cell populations in vitro

Davies et al (2011) PLoS One. Figure 1

NucleiGFAP (differentiation)Olig2 (injury)

bFGF BMP-4 CNTF

Markers of astrocyte differentiation/ astroglial lineage

Characterization of the cell populations in vitro

Davies et al (2011) PLoS One. Figure 1

Glial scar formation/ injury response

9W-1

9W-2

Transplant morphology: Human GDA survival and support of axon growth within DLF injuries

Davies et al (2011) PLoS One. Figure 2 and Suppl Figure 1; http://vanat.cvm.umn.edu/neurLab2/SpCdWhite.html

NF+ density:BMP: 14.79 ± 3.05CNTF: 7.75 ± 2.28

Cell migration and GFAP staining within injuries

Arrowheads indicate cell bodies

Davies et al (2011) PLoS One. Figure 3

Max rostral/caudal migration:CNTF: 3.24/ 3.96 mmBMP: 2.52/ 2.16 mm

Functional assessment after implantation of hGDAsBMP hGDAsCNTF or hGCPs

Grid walk test: http://www.pnas.org/content/suppl/2007/02/19/0611723104.DC1/11723Movie1.mov

Davies et al (2011) PLoS One. Figure 5

Neuroprotection and survival

Davies et al (2011) PLoS One. Figures 6 and 7

White = NeuN (neuron survival marker)

The Injury Model

Van den Brand et al (2012) Science. Suppl Figure 1

The Robotic Postural Interface

Van den Brand et al (2012) Science. Suppl Figure 2

Multi-System Neuroprosthetic Training System

Van den Brand et al (2012) Science. Suppl Figure 3

Locomotion after training

Van den Brand et al (2012) Science. Figure 1

Locomotion after training

Van den Brand et al (2012) Science. Supplementary Movie 1

http://www.sciencemag.org/content/suppl/2012/05/30/336.6085.1182.DC1/1217416s1.mov

Neural circuit remodeling

Van den Brand et al (2012) Science. Figure 2

Neural circuit remodeling

Van den Brand et al (2012) Science. Figure 3

Neural circuit remodeling

Van den Brand et al (2012) Science. Figure 4