Supplementary Data Indian Journal of Microbiology Mycobacterium aurum is unable to survive Mycobacterium tuberculosis latency associated stress conditions: Implications as non-suitable model organism Shivani Sood 1 • Anant Yadav 1 • Rahul Shrivastava 1* * Corresponding author Author Affiliations 1 Department of Biotechnology & Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan (Himachal Pradesh) - 173234, India Corresponding author Email ids: [email protected]
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Supplementary Data
Indian Journal of Microbiology
Mycobacterium aurum is unable to survive Mycobacterium tuberculosis latency associated stress conditions:
Supplementary Fig. S1 Ziehl-Neelsen staining of M. aurum cultures under actively replicating and different
stress conditions [1000X] Oil immersion. Alterations in staining were determined by staining M. aurum cells using
Ziehl-Neelsen method for acid fast bacteria (a) Actively replicating cells of M. aurum show normal cell morphology
and staining characteristics (bacilli stained dark pink in color and longer in size) (b) Cells under Non-replicating
persistent state cells when subjected to hypoxia induced stress, exhibiting altered staining characteristics (lost acid
fastness and appeared shorter in size) (c) M. aurum cells under nutrition depletion induced stress conditions,
exhibiting altered staining characteristics (weak acid fast and bacilli stained light pink in color and were shorter in
size) (d) M. aurum cells under acid induced stress conditions, cells displaying altered staining characteristics (bacilli
stained light pink and bluish in color owing to loss of acid fastness and were shorter in size).
(a) (b)
(c) (d)
Supplementary Fig. S2 Alteration in pigmentation of M. aurum on subjecting to acid induced stress conditions (a) pH 4.5: Cells showing loss of pigmentation (b) pH 5.5: Cells showing partial loss in orange pigmentation (c) pH 6.5: Actively replicating control cells with orange-yellow pigmentation.
