Date post: | 21-Nov-2014 |
Category: |
Health & Medicine |
Upload: | samir-mahmoud |
View: | 285 times |
Download: | 0 times |
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCopyright 2002 Allyn & Bacon
Dr /Samir Mahmoud Attia
Copyright 2002 Allyn & BaconCopyright 2002 Allyn & Bacon
By
Dr /Samir Mahmoud Attia
By
Dr /Samir Mahmoud Attia
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Aim of the work
• This essay aim to clarify the technique of
Banking of stem cells by different ways of collection and storing of blood stem cells
• This essay aim to clarify the technique of
Banking of stem cells by different ways of collection and storing of blood stem cells
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
What Are Stem Cells ?
Unspecialized, primitive Self-renewing cells that Can differentiate into cells with specific functions
Unspecialized, primitive Self-renewing cells that Can differentiate into cells with specific functions
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Classification of stem cell
Embryonic , adult
Hematopoietic, mesenchymal
Tottipotent,PleuripotentlMultipotentunipotent
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Embryonic stem cells
• Embryonic stem cells are only found naturally
in the early stages of embryonic development
and are totipotent i.e. they can form any type
of adult cell or adult cell precursor.
• Embryonic stem cells are only found naturally
in the early stages of embryonic development
and are totipotent i.e. they can form any type
of adult cell or adult cell precursor.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Adult stem cells
Have been found in:BrainBone marrowBlood vesselsDigestive tract
Are multipotent, e.g., hematopoietic stem cells that form different blood
components
Have been found in:BrainBone marrowBlood vesselsDigestive tract
Are multipotent, e.g., hematopoietic stem cells that form different blood
components
Skeletal muscleSkinLiverUmbilical cord
Skeletal muscleSkinLiverUmbilical cord
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Hematopoietic stem cells
A hematopoietic stem cell is a cell isolated
from the blood or bone marrow that can
renew itself.
It can differentiate to many specialized
cells.
A hematopoietic stem cell is a cell isolated
from the blood or bone marrow that can
renew itself.
It can differentiate to many specialized
cells.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Mesenchymal Stem Cells
Mesenchymal cells are distinct from
haematopoietic cells by being CD45 -.
Mesenchymal cells are a mixed cells that
are capable of supporting haematopoiesis
and differentiating into endothelial, bone,
muscle and neural cells.
Mesenchymal cells are distinct from
haematopoietic cells by being CD45 -.
Mesenchymal cells are a mixed cells that
are capable of supporting haematopoiesis
and differentiating into endothelial, bone,
muscle and neural cells.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
1. TOTIPOTENTIAL stem cells
Derived from embryonic stem cells
They can become any cell type
They can renew themselves indefinitely
1. TOTIPOTENTIAL stem cells
Derived from embryonic stem cells
They can become any cell type
They can renew themselves indefinitely
Classification Of Stem Cells
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
2. PLURIPOTENTIAL stem cells
can grow into any cell type except
totipotential stem cells. They cannot
become an embryo.
They can renew themselves indefinitely.
2. PLURIPOTENTIAL stem cells
can grow into any cell type except
totipotential stem cells. They cannot
become an embryo.
They can renew themselves indefinitely.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
3. MULTIPOTENTIAL stem cells
Generate only closely related cells, e.g.
blood cells such as white blood cells, red
blood cells, lymphocytes, platelets… etc.
They can renew themselves indefinitely.
3. MULTIPOTENTIAL stem cells
Generate only closely related cells, e.g.
blood cells such as white blood cells, red
blood cells, lymphocytes, platelets… etc.
They can renew themselves indefinitely.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
4. UNIPOTENTIAL (PROGENITOR) stem cells
Produce only one cell type They also
have the ability of self renewal, which
distinguishes them from non-stem cells.
4. UNIPOTENTIAL (PROGENITOR) stem cells
Produce only one cell type They also
have the ability of self renewal, which
distinguishes them from non-stem cells.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Sources of Stem Cells
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Peripheral Blood Stem Cells (PBSC)
The bloodstream is one source of stem
cells, although not rich as a source as bone
marrow.
To have enough stem cells in a donor's
bloodstream for a transplant, the donor is
given a special drug called a "growth
factor" (filgrastim is a drug that is
commonly used for this purpose).
The bloodstream is one source of stem
cells, although not rich as a source as bone
marrow.
To have enough stem cells in a donor's
bloodstream for a transplant, the donor is
given a special drug called a "growth
factor" (filgrastim is a drug that is
commonly used for this purpose).
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
When enough stem cells are present in the bloodstream, the donor undergoes a process called apheresis.
During an apheresis the blood stem cells are separated from the donor's blood, and the remaining blood goes back into the donor's bloodstream through a sterile needle.
When enough stem cells are present in the bloodstream, the donor undergoes a process called apheresis.
During an apheresis the blood stem cells are separated from the donor's blood, and the remaining blood goes back into the donor's bloodstream through a sterile needle.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Bone Marrow Stem Cells
Bone marrow, a spongy tissue found inside larger
bones, it is a rich source of blood stem cells.
Approximately one liter of marrow is needed for a
blood stem cell transplant, but the exact amount
needed depends on the size of the patient.
Marrow is removed during a surgical procedure.
Sterile needles and syringes are used to remove the
marrow from the pelvic bone.
Bone marrow, a spongy tissue found inside larger
bones, it is a rich source of blood stem cells.
Approximately one liter of marrow is needed for a
blood stem cell transplant, but the exact amount
needed depends on the size of the patient.
Marrow is removed during a surgical procedure.
Sterile needles and syringes are used to remove the
marrow from the pelvic bone.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Umbilical Cord Blood Stem Cells
Umbilical cord blood is another rich source of
stem cells.
Months before the baby's birth, the mother signs
an agreement to donate the umbilical cord blood
when the baby is born.
At birth, the cord blood unit is collected and
taken to a cord blood bank, where it is tissue-
typed, processed and stored frozen until needed
for a transplant.
Umbilical cord blood is another rich source of
stem cells.
Months before the baby's birth, the mother signs
an agreement to donate the umbilical cord blood
when the baby is born.
At birth, the cord blood unit is collected and
taken to a cord blood bank, where it is tissue-
typed, processed and stored frozen until needed
for a transplant.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Cord Blood Banking
The first successful cord cell transplant to a
sibling with Fanconi’s anemia took place in
1988.
This proven utility of cord blood led to the
establishment of cord blood banks. The first
private and public banks were established in
1992.
The first successful cord cell transplant to a
sibling with Fanconi’s anemia took place in
1988.
This proven utility of cord blood led to the
establishment of cord blood banks. The first
private and public banks were established in
1992.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
• CB banking provides rapid availability of
allogeneic donors for stem cell transplantation,
little donor risk or attrition, low risk of
transmitting infection, reduced or no risk of
(AGVHD) .
• CB banking provides rapid availability of
allogeneic donors for stem cell transplantation,
little donor risk or attrition, low risk of
transmitting infection, reduced or no risk of
(AGVHD) .
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Advantages and Disadvantages of
Umbilical Cord Blood
Advantages and Disadvantages of
Umbilical Cord Blood
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Advantages of UCB
1) Cord blood has been found to possess more primitive cells.
2) Umbilical cord blood offers potential advantages,that infectious diseases is marked less than bone marrow or peripheral blood despite the possibility of congenital and perinatal transmission of infectious agents. for example, no cord bloods would be infected with Epstein Barr virus (EBV).
1) Cord blood has been found to possess more primitive cells.
2) Umbilical cord blood offers potential advantages,that infectious diseases is marked less than bone marrow or peripheral blood despite the possibility of congenital and perinatal transmission of infectious agents. for example, no cord bloods would be infected with Epstein Barr virus (EBV).
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
3) CD34+ CD38- cells in umbilical cord blood also
proliferate more rapidly in response to cytokine
stimulation with IL-3, IL-6 and stem cell factor
(SCF) and generate seven times more
progenitor cells than do bone marrow .
3) CD34+ CD38- cells in umbilical cord blood also
proliferate more rapidly in response to cytokine
stimulation with IL-3, IL-6 and stem cell factor
(SCF) and generate seven times more
progenitor cells than do bone marrow .
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
4) Umbilical cord blood has more haematopoietic stem cells per volume than peripheral blood or bone marrow. In addition, umbilical cord blood seems more tolerant of HLA mismatches; with less graft versus host disease
5) Cord blood is less risky to collect. The collection of cord blood following delivery is a harmless process that does not affect the mother or her newborn.
4) Umbilical cord blood has more haematopoietic stem cells per volume than peripheral blood or bone marrow. In addition, umbilical cord blood seems more tolerant of HLA mismatches; with less graft versus host disease
5) Cord blood is less risky to collect. The collection of cord blood following delivery is a harmless process that does not affect the mother or her newborn.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Disadvantages of UCB
1) A disadvantage of umbilical cord blood
compared with donated adult bone marrow is
that newborns may carry undiagnosed genetic
diseases.
2) BM donors can be recalled for the same patient
to provide a second donation of BM.
1) A disadvantage of umbilical cord blood
compared with donated adult bone marrow is
that newborns may carry undiagnosed genetic
diseases.
2) BM donors can be recalled for the same patient
to provide a second donation of BM.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
3) BM donors can provide a medical history, which
is relevant to the donation, at the time of
donation. However, CB donors provide the
medical history through their mothers.
4) that bone marrow contains more mesenchymal
progenitor cells than CB.
3) BM donors can provide a medical history, which
is relevant to the donation, at the time of
donation. However, CB donors provide the
medical history through their mothers.
4) that bone marrow contains more mesenchymal
progenitor cells than CB.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Bone Marrow/ Peripheral Blood
Cord Blood
-Donation requires surgery under general anesthesia (in B.M) -Donors feel discomfort and/or pain (in P.B). -Long-term consequences of growth factors used in peripheral blood stem cell
donation posses no risk
to mother or infant.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Large dose of stem cells. Rapid engraftment.
Smaller dose of stem cells. Slower engraftment.
After a formal search
is begun, takes an
average of 4 months
to transplantion, if a
donor is available.
When a match is
found, can take only
a few days for
confirmatory and
special testing (may
reach less than 24
hours in an
emergency).
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Potential donors may no longer be available or may have withdrawn consent. Donor must be found and retested to confirm the HLA typing and infectious disease results and to confirm that the donor is still willing and able to donate bone marrow .
Once frozen, a cord
blood unit is available
until used.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Bone marrow must be used fresh (shelf-life measured in hours). Peripheral blood stem cells stored for short term (days to a few months).
Frozen cord blood has been transplanted successfully up to 10 years in storage.
Latent viral infection in the donor common (i.e. CMV > 50% in adult donors).
Latent viral infection in the cord blood donor rare (i.e. CMV <1% )
Severe graft vs host disease (GvHD) common.
GvHD less frequent and easier to treat.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Generally requires a perfect match between donor and recipient for HLA-A, -B . Additional factors HLA-C, -DQ and -DP)are needed to improve prognosis).
HLA-mismatched cord blood transplantion are possible, making it easier to find a suitable match.
No risk of transplanting a genetic disease.
Cord blood has a small risk of transplantation rare unrecognized genetic disease.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Methods of umbilical cord blood collection
• variety of potential collection methods (open,
semi- closed or closed methods) have been
proposed in order to optimize the collection
volume and reduce the risks of microbial and
maternal contamination.
• variety of potential collection methods (open,
semi- closed or closed methods) have been
proposed in order to optimize the collection
volume and reduce the risks of microbial and
maternal contamination.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Donor selection
• The medical history interview includes a
review of the risk for HIV and hepatitis B
and C, including skin piercing and blood
transfusion, as well as the presence of
infections that can be transmitted.
• The medical history interview includes a
review of the risk for HIV and hepatitis B
and C, including skin piercing and blood
transfusion, as well as the presence of
infections that can be transmitted.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Collection of UCB
• Method of Collections :
• 1-In- utero: before delivery of placenta
• 2-Ex- utero:After placental delivery
• Method of Collections :
• 1-In- utero: before delivery of placenta
• 2-Ex- utero:After placental delivery
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
• Representation of the UCB collection system. The delivered placenta is placed onto the funnel-shaped supporting surface with the maternal side facing upward, the fetal side facing downward, and the umbilical cord passing through the central hollow cylinder of the supporting surface. The device lid is then closed, the pressure application system is turned on, and the UCB collection begins.
• Representation of the UCB collection system. The delivered placenta is placed onto the funnel-shaped supporting surface with the maternal side facing upward, the fetal side facing downward, and the umbilical cord passing through the central hollow cylinder of the supporting surface. The device lid is then closed, the pressure application system is turned on, and the UCB collection begins.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
• Blood collection was made by puncturing the umbilical vein after the umbilical cord had been clamped, cut, and cleaned with an antiseptic solution, and drainage by gravity into a regular blood donation triple-bag system with the anticoagulant citrate phosphate dextrase (CPD) reduced to 25 mL
• Blood collection was made by puncturing the umbilical vein after the umbilical cord had been clamped, cut, and cleaned with an antiseptic solution, and drainage by gravity into a regular blood donation triple-bag system with the anticoagulant citrate phosphate dextrase (CPD) reduced to 25 mL
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Collection of UCB
• Volume
80 – 120 mL. (30-40 mL..TNC > 6 x 10/9 cells)
• Transport
–By dry shipper( Refrigeration 4 C)
• Volume
80 – 120 mL. (30-40 mL..TNC > 6 x 10/9 cells)
• Transport
–By dry shipper( Refrigeration 4 C)
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Processing of UCB
• 24-36 hrs. post collection.– Max. 48 hrs.
– Volume reduction
• depletion of erythrocytes and/or plasma by:
• HES (hydroxy ethyl starch)
• Hespan. (1:5 ratio Hespan/UCB)
• 24-36 hrs. post collection.– Max. 48 hrs.
– Volume reduction
• depletion of erythrocytes and/or plasma by:
• HES (hydroxy ethyl starch)
• Hespan. (1:5 ratio Hespan/UCB)
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Processing of UCB
• Analysis.– Cell Count/Diff, Viability (>70%), ABO,
Rh,
– CD34 +, Stem Cell Assay (post processing)
– Sterility Testing (pre and post processing).
• Analysis.– Cell Count/Diff, Viability (>70%), ABO,
Rh,
– CD34 +, Stem Cell Assay (post processing)
– Sterility Testing (pre and post processing).
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Processing of UCB
• Cryopreservation
– 25 –50 mL Cryocyte Storage Container
– 10% dimethyl sulfoxide (DMSO)
– Control Rate Freeze Process
• 1 C – 2 C /min
• Cryopreservation
– 25 –50 mL Cryocyte Storage Container
– 10% dimethyl sulfoxide (DMSO)
– Control Rate Freeze Process
• 1 C – 2 C /min
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Most cord blood units were cryopreserved
as whole blood donations with 10% DMSO
and transfused immediately after thawing
without any attempt to remove the
cryoprotectant.
Most cord blood units were cryopreserved
as whole blood donations with 10% DMSO
and transfused immediately after thawing
without any attempt to remove the
cryoprotectant.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Thawing of cord blood units is usually performed
by placing the cord blood bags in the gas phase
of liquid nitrogen for 30 min followed by a 5-min
exposure to room temperature.
The bags are then thawed in a 37C water bath
as rapidly as possible.
Immediately after thawing, each cord blood unit
is diluted with an equal volume of solution
containing 5% human serum albumin and 10%
Thawing of cord blood units is usually performed
by placing the cord blood bags in the gas phase
of liquid nitrogen for 30 min followed by a 5-min
exposure to room temperature.
The bags are then thawed in a 37C water bath
as rapidly as possible.
Immediately after thawing, each cord blood unit
is diluted with an equal volume of solution
containing 5% human serum albumin and 10%
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Dextran 40 in 0.9% NaCl, and sedimented for
10 min. The supernatant is then removed and
sedimented cells are resuspended slowly in
fresh albumin/ dextran solution.
• This procedure removes the bulk of RBC ghosts,
hemoglobin, and DMSO, thus reducing some of
the risks associated with the transplantion
procedure.
Dextran 40 in 0.9% NaCl, and sedimented for
10 min. The supernatant is then removed and
sedimented cells are resuspended slowly in
fresh albumin/ dextran solution.
• This procedure removes the bulk of RBC ghosts,
hemoglobin, and DMSO, thus reducing some of
the risks associated with the transplantion
procedure.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Recommindations
• 1-Written permission should be obtained during prenatal care and before the onset of labour.
• 2-cord blood collection should not be done in complicated deliveries and the cord blood stem cell collection program should not alter routine practice for the timing of umbilical cord clamping.
• 1-Written permission should be obtained during prenatal care and before the onset of labour.
• 2-cord blood collection should not be done in complicated deliveries and the cord blood stem cell collection program should not alter routine practice for the timing of umbilical cord clamping.
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
• 3-condition such as leukemia or severe hemoglobinopathy may indicate the need for directed donor cord blood banking for sibling cord blood transplantation.
• 4-preliminary data show encouraging results in cord blood stem cell transplantation for a variety of genetic, hematological and oncological diseases .
• 3-condition such as leukemia or severe hemoglobinopathy may indicate the need for directed donor cord blood banking for sibling cord blood transplantation.
• 4-preliminary data show encouraging results in cord blood stem cell transplantation for a variety of genetic, hematological and oncological diseases .
WEB
IRM
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW
Copyright 2002 Allyn & BaconCustomized by Marsha Blachman, LCSW