Date post: | 26-Jan-2015 |
Category: |
Technology |
Upload: | jennystolle |
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Strawberry DNAExtraction
Plant and
Animal Cells
DNA is found in the Nucleus
From DNA to Organism
Proposed Procedure
As a class, lets come up with a procedure to extract the DNA from the nucleus of the plant cell.
What should we do first?1. First?2. Second?3. Third?4. Fourth?
• SMASH the Strawberry
First Step:Let’s play with our food! Smash the strawberry as best you can WITHOUT breaking the bag.We need to break apart the cell walls to get inside the cell.
Know your Equipment
TEST TUBE
GRADUATED CYLINDER
Funnel
• Add Soap Buffer
Dissolve the MembraneAdd 10ml of extraction buffer to the bag.
Seal the bag and start to smash the strawberry for 1 more min.
We need to dissolve the membrane around the nucleus just like we dissolve the fat from frying pans.
•Filter the Strawberry/Extra
ction MixtureSLOWLY and CAREFULLY pour the mixture through the gauze in the funnel into the test tube.
While letting it drip, draw your extraction set up in the designated box.
We need to strain the cell fragments from the DNA to get a good extraction.
Extraction Set-Up
Funnel
Test Tube
Strawberry Mixture
Paper Towel
•Clump the DNAAdd COLD Ethanol
Now let’s pull the DNA out.Hold out your test tube with the filtered mixture.The teachers will come around and pour COLD ethanol into your test tube.OBSERVE what happens!
Ethanol LayeringDo not mix!