Stressed Selected Human Mesenchymal Stem CellsPotent, Pure and Easy to Culture

What makes these cells unique?

New Stress Select Human Stem Cells-They Survive to ThriveCapabilities:

•High potency, expansion rates and passaging.

•Low telomerase activity=low tumorigenesis properties

•High ability to migrate and differentiate to specific tissue types-High expression of migration/homing chemokines

Image: We tested the effects of cellular stress on human CB-MSCs (Neuromics’, SC00A1) since others have reported conversion to the pluripotent state (Heneidi, S., et al., PLoSOne 8: e64752, 2013). We recovered human CB-MSCs from cryopreservation and briefly cultured these cells (48 hours) at 20% O2-5% CO2 in MSC-Gro, low-serum medium (Neuromics, SC00B1). As a stressful environment, these cells were then transferred to 1% O2-5% CO2 at 105/ml in nutrient-poor medium (expired RPMI) supplemented with 0.12 PZ/ml collagenase NB 4 (Serva, catalog number 17454) at 4oC. We retrieved viable cells 48-hours later and transferred them to MSC-Gro humanized, complete medium (Neuromics’, SC00B2). The images above show formation of multi-cell clusters ~50 to 130 um at 2 days (Left) and 4 days (Rt) following exposure to stress. Scale bar is 50 mm.

Image: Further morphology of these cultures is shown above as phase contrast images of two prominent clusters together with more firmly attached cells of classical mesenchymal morphology. This is day seven following exposure to stress. Scale bar is 50 um.

Images: (A) Clusters of stress-selected cells are positive for SSEA-3, a pluripotency marker. Following blockage of non-specific binding, cultures were incubated with a Alexa Fluor-conjugated SSEA-3 monoclonal antibody (MC-631-AF 488; BioVision, catalog number 3068-25T) at 10 ml/ml overnight. (B)SSEA-3 positive cells within cell clusters together with propidium iodide. Red cells are non-viable. SSEA-3 is specific to embryonic stem cells; MSCs were negative for SSEA-3.

Stress Selected Stem Cells Introductory offer-Save 150 USD

Ordering Options

600 USD for a vial of 500,000 cells.