Studies on the cestode genus silurotaenia, Nybein, 1942 from mystus seenghala

(Ham. and Buch.) with description of a new speciesDHANRAJ BALBHIM BHURE, D.M. PATHAN, SANJAY SHAMRAO NANWARE AND R.M. DHONDGE

The Asian Journal of Animal Science (December, 2010), Vol. 5 Issue 2 : 205-208

Key words : Mystus seenghala, Silurotaenia, Taxonomic observation.

ABSTRACT

The present communication deals with the taxonomic observation of piscian tapeworm

Silurotaenia raoii Sp.Nov. collected from intestine of a Mystus seenghala (Ham. and Buch.) at

Osmanabad district of (M.S.) India. The worm comes closer to all the known species of this

genus in general topography of organ but differs due to scolex pear shaped, suckers four

,arranged in two groups, rostellum medium, oval, bearing numerous rostellar hooks, neck

absent, mature proglottids broader than long, testes 125-130 in numbers, pre-ovarian in position

, cirrus pouch oval, ovary distinctly bilobed, vagina lies posterior to the cirrus pouch, genital

pores oval in shape, marginal and irregularely alternate and vitellaria granular.

See end of the article for

authors’ affiliations

DHANRAJ BALBHIM BHURE

Research and Post Graduate

Department of Zoology

Yeshwant Mahavidyalaya,

NANDED (M.S.) INDIA

draj_bhure@yahoo.co.in

RESULTS AND DISCUSSION

The results are summarized below according to the

objective of the study :

Description (Based on four specimens, Fig. 1 and 2):

All cestodes are long, consisting of scolex, immature,

mature and gravid proglottids and measures 42-47 mm in

length and 1-4 mm in width. Scolex well developed,

distinct longer than broad, pear shaped and measures

0.279 (0.242-0.315) in length and 0.266 (0.233-0.300) in

breadth. Scolex bears pair of suckers, arranged in two

pairs, overlapping with each other, oval to rounded and

measures 0.080 (0.072-0.087) in length and 0.065 (0.053-

0.077) in breadth. Rosetellum medium, oval, present at

the anterior extremity of the scolex and measures 0.126

(0.087-0.165) in length and 0.213 (0.179-0.247) in breadth.

Rostellum is surrounded by number of hooks, which are

arranged in circle, ‘V’ shaped, and measures 0.019(0.014-

0.024) in length and 0.009(0.004-0.014) in breadth. Neck

is absent.

Mature proglottids are broader than long and

measures 0.958 (0.883-1.03) in length and 1.759 (1.723-

1.80) in breadth. Testes small, rounded, evenly distributed

all over the proglottid, 125-130 in numbers, pre-ovarian

and measures 0.0024 (0.014-0.033) in length and 0.021

(0.014-0.029) in breadth. Cirrus pouch oval, transversely

placed and measures 0.269 (0.252-0.286) in length and

0.106 (0.087-0.126) in breadth. Cirrus thin tube, within

the cirrus pouch and measures 0.237 (0.223-0.252) in

length and 0.012 (0.009-0.014) in breadth. Vas deferens

thin, tubular, slightly curved, runs obliquely up to testicular

field and measures 0.131 (0.121-0.140) in length and 0.012

The genus Silurotaenia was erected by Nybin, 1942

as S. siluri from Siluris glanis. Shinde, Deshmukh

and Chincholikar, 1975 described S. nybelin from

Pseudeutropius taakree. In 1984 Shinde et al. (1984)

added four new species to this genus i.e. S. macroni, S.

seenghala from Macrones seenghala and S. barbusi,

S. ticto from Barbus ticto. In 1989, Deshmukh and Shinde

added S. behairvnathi, new species to this genus. Later

on, Jadhav et al. (1991) added one more new species to

this genus, i.e. S. shastri from M. seenghala. In 2002,

Wankhede and Jadhav reported S. godavari from

Sparata seenghala.

MATERIALS AND METHODS

Eighty five specimens of cestode parasites were

collected from the intestine of freshwater fish Mystus

seenghala (Ham. and Buch.) at Osmanabad districts of

Maharastra, India during the period of Feb., 2005 to Jan.,

2007. These cestodes are preserved in hot 4% formalin

and four specimens are stained with Harris haematoxylin

and Borax carmine, passed through various alcoholic

grades, cleared in xylene, mounted in D.P.X. and drawings

are made with the aid of Camera lucida and all

measurements are recorded in millimeters unless

otherwise mentioned.

•HIND AGRICULTURAL RESEARCH AND TRAINING INSTITUTE•

Bhure, Dhanraj Balbhim, Pathan, D.M., Nanware, Sanjay

Shamrao and Dhondge, R.M. (2010). Studies on the cestode

genus silurotaenia, Nybelin, 1942 from mystus seenghala (Ham.

and Buch.) with description of a new species. Asian J. Animal

Sci., 5(2): 205-208

RESEARCH ARTICLE

Received : Aug., 2010; Accepted : Nov., 2010

•HIND AGRICULTURAL RESEARCH AND TRAINING INSTITUTE•[Asian. J. Animal Sci. (Dec., 2010) Vol. 5 (2) ] 206

(0.009-0.014) in breadth.

Vagina thin, arises from posterior side of the cirrus

pouch, forms receptaculum seminis and measures 0.684

(0.674-0.696) in length and 0.012 (0.009-0.014) in breadth.

Receptaculum seminis is thin, runs transversely towards

the posterior side, reaches to ootype and measures 0.434

(0.427-0.441) in length and 0.024 (0.014-0.033) in breadth.

Vagina and cirrus pouch open through the common genital

pore which is oval, marginal, irregularly alternate and

measures 0.058 (0.053-0.063) in length and 0.014 (0.009-

0.019) in breadth. Ootype small, oval in shape and

measures 0.097 in diameter. From the ootype ovarian

lobes start. Ovary large, bilobed, located at the posterior

side of the segment and measures 1.376 (1.34-1.40) in

length and 0.162 (0.111-0.213) in breadth. Excretory

canals long, tubular, running across the proglottid

longitudinally on both sides of the proglottid and measures

0.917(0.898-0.936) in length and 0.016 (0.014-0.019) in

breadth. Vitellaria granular, arranged on either side of the

proglottid, pre-ovarian, except the cirrus pouch.

The genus Silurotaenia was established by Nybelin,

1942 with its type species S. siluri. The present cestode

comes closer to all the known species of the genus

Silurotaenia Nybelin, (1942) in general topography of

organs but differs due to some characters from following

species.

– The present tapeworm differs from S. siluri

Nybelin, (1942) in having mature proglottids almost

squarish, testes 220-230 in numbers, cirrus pouch oval,

genital pores just anterior to the middle of the segment,

vitellaria follicular, 3-4 in rows and reported from Silurus

glanis, in Europe.

– The present cestode differs from S. nybelini

Shinde et al. (1975) in possessing scolex bluntly pointed

at anterior end, mature proglottids longer than broad,

testes 130-140 in numbers, genital pore placed one third

from the anterior margin of the segment and reported

from Pseudotropius taakree, in India.

– The present worm S.raoii Sp.Nov. differs from

S. macroni Shinde et al. (1984) in having scolex long and

large, mature proglottids almost squarish, testes 68 in

numbers, genital pore placed in the middle position of the

proglottids and reported from Macronus seenghala, in

India.

– The present parasite differs from S. seenghala

Shinde et al. (1984) due to scolex long with deep

constriction on both the sides, testes 370-390 in numbers,

cirrus pouch sacular, elongated, genital pore placed one

third from the anterior margin of the proglottids, vitellaria

follicular and reported from Macronus seenghala, in

India.

– The present form differs from S. barbusi Shinde

et al. (1984) in having scolex large, mature proglottids

longer than broad, testes 135-140 in numbers, cirrus pouch

cylindrical and reported from Barbus ticto, in India

– The present cestode S.raoii Sp.Nov. differs from

S. ticto Shinde et al. (1984) in having scolex broader at

the base and tapering anteriorly, testes 575-590 in numbers,

cirrus pouch large, saccular, spinose, genital pore just

anterior to the middle of the segment and reported from

Barbus ticto, in India.

– The present parasite differs from S. behairvnathi

Deshmukh et al. (1989) in having scolex distinct, longer

than broad, mature proglottids almost squarish, testes 250-

260 in numbers, spherical, cirrus pouch oval, genital pore

at one fourth of the proglottids and reported from

Mastacembelus armatus, in India

DHANRAJ BALBHIM BHURE, D.M. PATHAN, SANJAY SHAMRAO NANWARE AND R.M. DHONDGE

Fig. 1: Micro-photograph of Senga madhavae Sp. Sov.

Scolex Rostellum with hooks Mature Proglottids

•HIND AGRICULTURAL RESEARCH AND TRAINING INSTITUTE•[Asian. J. Animal Sci. (Dec., 2010) Vol. 5 (2) ] 207

– The present parasite differs from S. shastri Jadhav

et al. (1991) in having scolex vessel shaped, small, testes

72-78 in numbers, oval to rounded, cirrus pouch oval,

narrow anteriorly and broad posteriorly, genital pore small,

rounded and reported from Macronus seenghala, in

India

– The present worm S.raoii Sp.Nov. differ from S.

godavari Wankhede and Jadhav (2002) in having scolex

small, quadrangular, mature proglottids squarish, testes

220-230 in numbers, genital pore small, sub-marginal,

unilateral, rounded, at anterior half of the proglottids and

reported from Separta seenghala, in India

In above discussion on the present parasite deserves

the status of a new species and named Silurotaenia raoii

Sp.Nov. proposed in honour of Prof. K.Hanumanth Rao,

Department of Zoology, Andhra University,

Visakhapattnam, an eminent Parasitologist.

Taxonomic summary:

Genus Silurotaenia Nybelin,1942

Species Silurotaenia raoii Sp.Nov.

Type host Mystus seenghala (Ham. and

Buch.)

Habitat (Site) Intestine

Type locality India, Maharashtra (Osmanabad

district.)

Accession Number HRL/2005-07/4/1-4

Holotype and Deposited in the Helminthology

Research Lab., Department of

Zoology,

STUDIES ON THE CESTODE GENUS SILUROTAENIA, NYBELIN, 1942 FROM MYSTUS SEENGHALA (HAM. & BUCH.) WITH DESCRIPTION OF A NEW SPECIES

0.5 mm

A,B

A B

Scolex Mature Proglottids

Fig. 2: Camera lucida diagram of Silurotaenia raoii Sp. Nov.

A key to the species of the genus Silurotaenia Nybein, 1942

Mature segment squarish 1

Mature segment longer than broad 2

Mature segment broader than long 3

1. Testes below 100 in numbers S. macroni Shinde et al., 1984

Testes in between 100-250 in numbers 4

Testes above 250 in numbers S. behairvnathi Deshmukh et al.,1989.

2. Cirrus pouch oval in shape S. nybelini Shinde et al.,1975

Cirrus pouch cylindrical S. barbusi Shinde et al.,1984

Testes below 100 in numbers S. shastri Gavane et al.,1991

3. Testes in between 100- 200 in numbers S. raoii Sp.nov.

Testes in between 300-400 in numbers S. seenghala Shinde et al.,1984

Testes above 400 in numbers S. ticto Shinde et al.,1984

4. Vitellaria granular S. godavari Wankhede and Jadhav, 2002

Vitellaria follicular S. siluri Nybelin, 1992

•HIND AGRICULTURAL RESEARCH AND TRAINING INSTITUTE•[Asian. J. Animal Sci. (Dec., 2010) Vol. 5 (2) ] 208

Paratype Dr. B.A.M. University,

Aurangabad, (M.S.) India.

Date of collection Feb., 2005-Jan., 2007.

Etymology Named in Honour of Prof. K.

Hanumanth Rao,

Department of Zoology,

Department of Zoology,

Andhra University,

Visakhapattnam an eminent

Parasitologist.

Authors’ affiliations:

D.M. PATHAN, Department of Zoology, Shrikrishna

Mahavidyalaya, Omerga, OSMANABAD (M.S.) INDIA

SANJAY SHAMRAO NANWARE, Research and

Post Graduate Department of Zoology, Yeshwant

Mahavidyalaya, NANDED (M.S.) INDIA

Email: snanware@rediffmail.com

R.M. DHONDGE, Department of Zoology, Shri Sant

Gadge Maharaj Mahavidyalaya, NANDED (M.S.)

INDIA

LITERATURE CITED

Deshmukh, S.B. and Shinde, G. B. (1989). On a new species,

Silurotaenia behairvnathi sp. nov. (Cestoda-Proteocephalidae)

from a freshwater fish, Mastacembelus armatus from

Aurangabad, M. S. Fotedar Commemoration , pp. 75-77.

Jadhav, B.V., Ghavane, A.B. and Jadhav, A.P. (1991). On a new

Pseudophylidean cestode from Mastacembelus armatus at

Daryapur (M.S.), India. Rivista Di Parasit., 8(1):19-22.

Nybein, O. (1942). Zuer Helminth Fauna der Sussawasser Fische

Schwedens II. Die cestode, des welses.Goteboogs Kgl.

Vetenskaps-Akad Handl. Sect. B.L. 1-24.

Shinde, G.B., Deshmukh, R.A. and Chincholikar, L.N. (1975).

On a new cestode, Silurotaenia nybelini n .sp.

(Proteocephalidae) from a freshwater fish from Marathwada,

India. Marathawada. Univ. J. Sci. 14(7): 355-359.

Shinde, G.B., Majid, M.A. and Solunke, D.G. (1983). On a new

species of Silurotaenia Nybelin, 1942 (Cestoda:

Proteocephalidae) from a freshwater fish at Paithan, M.S. India.

Riv.Di. Parasitol., 44: 309-312.

Shinde, G.B., Jadhav, B.V. and Kadam (1984): A revision of the

genus Silurotaenia Nybelin, 1942, with four new species. Riv.

Di. Para. Vol. I (XLV):83-91.

Wankhede, Hemlata and Jadhav, Baba (2002). On a new species

of the genus Silurotaenia Nybelin 1942 from Sperata seenghala

(Sykes). Indian J. Helminth. (N. S.)., 20: 11-14

**************

DHANRAJ BALBHIM BHURE, D.M. PATHAN, SANJAY SHAMRAO NANWARE AND R.M. DHONDGE