Supplementary table 1. Study group characteristics and frequencies of T cells specific for HIV- Gag and -Nef,determined by IFN-g ELISpot assaya

Total (n¼53) HLA-B57/5801þ (n¼11) HLA-B57/5801� (n¼11) Pc

CD4 count (cells/mm3) 639 (489–751) 696 (496–756) 611 (435–757) 0.365HIV-DNAb (log10/106 PBMCs) 2.29 (1.53–2.89) 1.76 (1.31–2.16) 2.79 (2.23–2.84) 0.065Plasma HIV-RNA (log10/mL) 3.78 (2.57–4.56) 3.16 (2.54–4.21) 4.25 (3.30–5.17) 0.193Frequencies of specific T cells (SFC/106PBMCs)Gag 2,527 (1,153–3,837) 3,093 (1,964–3,898) 2,739 (1,931–3,679) 0.847Nef 480 (198–1,270) 573 (389–1,270) 1,152 (672–1,762) 0.243

aAll data are presented as median and interquartile range (IQR). bHIV-DNA data were available for 50 patients. cComparisons between HLA-B57/5801þ and HLA-B57/5801- group.

Supplemental table 2. Correlations between virus burden and CD27 expression on HIV-specific CD8þTCM or TEMcells.

CD27 expression on TCM CD27 expression on TEM

Gag-specific Nef-specific Gag-specific Nef-specific

HLA-B57/5801þ group HIV-DNA r¼�0.683 r¼�0.417 r¼�0.333 r¼�0.400P¼0.042 P¼0.265 P¼0.381 P¼0.286

HIV-RNA r¼�0.389 r¼�0.274 r¼�0.140 r¼�0.213P¼0.266 P¼0.444 P¼0.700 P¼0.555

HLA-B57/5801- group HIV-DNA r¼0.060 r¼0.263 r¼�0.667 r¼�0.333P¼0.888 P¼0.528 P¼0.071 P¼0.420

HIV-RNA r¼�0.432 r¼�0.140 r¼�0.455 r¼�0.467P¼0.213 P¼0.700 P¼0.187 P¼0.174

SUPPLEMENTAL FIGURE 2. Functional avidity ofCD8þ T cells directed against HIV-Gag or Nef epitopesrestricted by HLA-B57 or other HLA alleles in two HLA-B57/5801þ (upper panel) and two HLA-B57/5801-

(lower panel) nonprogressors. Arrows represent thepeptide concentration providing half the maximalfrequency of IFN-g producing T cells (IC50). Closeand open symbols represent Gag and Nef epitopesrecognition respectively.

SUPPLEMENTAL FIGURE 1. Frequencies of CD8þ

T cells specific for HIV-Gag, and -Nef from 11 HLA-B57/5801þ and 11 HLA-B57/5801- HIV-infected non-progressors.

SUPPLEMENTAL FIGURE 3. Comparative IFN-g and IL-2 production by CD8þ T cells specific for HIV-Gag orNef. A, Results from intracellular cytokine staining after stimulation with HIV-Gag and Nef peptide pools gated onCD8þ T cells shown for two representative nonprogressors: one HLA-B57/5801þ (upper panel) and one HLA-B57/5801� (lower panel) nonprogressors. B. Profiles of IFN-g and IL-2 production by CD8þT cells against HIV-Gag and Nefantigens obtained by intracellular staining in 22 patients including 11 HLA-B57/5801þ (close symbols) and 11 HLA-B57/5801� individuals (open symbols).

SUPPLEMENTAL FIGURE 4. A, Memory and effector differentiation status of Gag- or Nef-specific IFN-g-producing CD8þ cells including CD45RA�CCR7� effector memory cells, CD45RA�CCR7þ central memory andCD45RAþCCR7� effector cells in HLA-B57/5801þ (close symbols) and HLA-B57/5801� (open symbols) individuals.B, CD45RA and CD27 expression on Gag- and Nef-specific IFN-gþCD8þ T cells shown for two representativenonprogressors: one HLA-B57/5801þ (left panel) and one HLA-B57/5801- (right panel) nonprogressors.

SUPPLEMENTAL FIGURE 5. CD27 expression on tetramer stained CD8þ T cells in 3 HLA-B57/5801þ subjects.HLA-B57/KF11 tetramer staining and phenotyping performed directly ex vivo and after overnight stimulation with theKF11 peptide.