MSc in Biology and Technology of Reproduction in Mammals SUBJECT: MORPHOLOGY AND FUNCTION OF THE MALE REPRODUCTIVE ORGANS (Compulsory subject, 6 ECTS) Begin: 18 th – 29 th September Subject coordinated by: Dr. Octavio López Albors Lecture room: Veterinary Faculty -1.2 Anatomy Cellular biology Physiology Common tasks 1st WEEK MONDAY 18 TUESDAY 19 WEDNESDAY 20 THURSDAY 21 FRIDAY 22 Morning 10:00 Lecture room Veterinary Facutly) Welcome & General Overview (Dr. M. Jiménez Dr. R. Romar Dr. O. López) Introduction e Morphology & Function Package (Dr. O López) 9:30-12:00 Practice. Dissection room Comparative anatomy of the pelvis and the male reproductive organs in domestic mammals (Drs. Mª D Ayala & G. Ramírez) 10:00 Theory Cel Biol Med Practice Room Fac. Medicine (hall 2 floor) Histology & cell biology: Spermiogenesis (Dr.M. Aviles) Sperm morphology (Dr.M. Aviles) 10:00 Theory Cel Biol Med Histology & cell biology Comparative sperm morphology (Dr. M Aviles Dr. Maria José Izquierdo Rico) 9:30 Theory. Lecture room Testicle physiology (Dr. S. Cánovas 15:30 Theory Lecture Room Physiology of the epididymis and reproductive glands (Dra. J Gadea) 12:00 Theory Lecture room Anatomy of the pelvis and general patterns of blood and nerve supply to the reproductive organs (Dr. Mª D. Ayala) Lunch Break Afternoon 15:30 Theory Lecture room Comparative anatomy of the male reproductive organs in domestic mammals (Dr. Mª D. Ayala) 16:00 Practice Dissection room Comparative anatomy of the pelvis and the male reproductive organs in domestic mammals (Drs. R. Latorre & G. Ramírez) 16:00 Theory. Cel Biol Med Practice Room Fac. Medicine (hall 2 floor) Histology & cell biology: Testicle (Dr JF Madrid) 16::00 Theory. Cel Biol Med Practice Room Histology & cell biology: Sperm ducts Accessory Reproductive Glands. (Dr.JF Madrid)
Transcript
MScinBiologyandTechnologyofReproductioninMammals
SUBJECT: MORPHOLOGY AND FUNCTION OF THE MALE REPRODUCTIVE ORGANS (Compulsory subject, 6 ECTS)
Begin: 18th – 29th September Subjectcoordinatedby:Dr.OctavioLópezAlbors
10:00 Lecture room Veterinary Facutly) Welcome & General Overview (Dr. M. Jiménez Dr. R. Romar Dr. O. López) Introduction e Morphology & Function Package (Dr. O López)
9:30-12:00 Practice. Dissection room Comparative anatomy of the pelvis and the male reproductive organs in domestic mammals (Drs. Mª D Ayala & G. Ramírez)
10:00 Theory Cel Biol Med Practice Room Fac. Medicine (hall 2 floor) Histology & cell biology: Spermiogenesis (Dr.M. Aviles) Sperm morphology (Dr.M. Aviles)
10:00 Theory Cel Biol Med Histology & cell biology Comparative sperm morphology (Dr. M Aviles Dr. Maria José Izquierdo Rico)
9:30 Theory. Lecture room Testicle physiology (Dr. S. Cánovas 15:30 Theory Lecture Room Physiology of the epididymis and reproductive glands (Dra. J Gadea) 12:00 Theory
Lecture room Anatomy of the pelvis and general patterns of blood and nerve supply to the reproductive organs (Dr. Mª D. Ayala)
Lunch Break
Afternoon
15:30 Theory Lecture room Comparative anatomy of the male reproductive organs in domestic mammals (Dr. Mª D. Ayala)
16:00 Practice Dissection room Comparative anatomy of the pelvis and the male reproductive organs in domestic mammals (Drs. R. Latorre & G. Ramírez)
16:00 Theory. Cel Biol Med Practice Room Fac. Medicine (hall 2 floor) Histology & cell biology: Testicle (Dr JF Madrid)
16::00 Theory. Cel Biol Med Practice Room Histology & cell biology: Sperm ducts Accessory Reproductive Glands. (Dr.JF Madrid)
MScinBiologyandTechnologyofReproductioninMammals
MONDAY 25 TUESDAY 26 WEDNESDAY 27
THURSDAY 28 FRIDAY 29 MONDAY 2 Oct
Morning
8:00 Practice Veterinary farm Ejaculate obtaining in buck (Dr. J Gadea) 11:30 Practice Lab Physiol Analysis of ejaculated samples (Dr. J. Gadea)
9:30 Theory Lecture Room Puberty and sexual behaviour (Dr. J. Gadea).
(off)
Opening Day
Academic
Year
9:30 Practice Lab Physiol Epididymal fluid obtaining in pig and bull (Dr. S Cánovas) 12:30 Info. session Lab physiol - Practicum - TFM
(Dr. R. Romar)
9:30 Practice Lecture room Bibliographic search and references management (Dr. C. Chaín) 11:30 Lecture room Seminars assignement (Dr. O. López)
9:30 Theory. Lecture room Anatomy of the male reproductive organs (human). Dr. K de Jong)
11:30 Practice Cel Biol Med Practice Room Electron microscopy of testicle, spermatic ducts and accessory glands) (Dr. M. Jiménez)
Break
Afternoon
15:30 Practice Cel Biol Med Practice Room Cel Biol Med Testicle and epididymis (light microscopy) (Dra. C.Ferrer y JF Madrid)
15:30 Practice Dissection room Anatomy of the male reproductive organs (human). (Dr. K de Jong)
SEMINARS
1. Sperm maturation (Dr. M Avilés)
2. Testicle thermoregulation: anatomical and physiological aspects (Drs. O López, J. Gadea)
3. Seminal plasma composition (Dr. S. Cánovas)
4. Erection: anatomy and physiology (Drs. O. López, J Gadea
5. Ejaculation: anatomy and physiology (Drs. O. López, J Gadea):
6. Hypophysis: comparative anatomy and histology (Drs. O. López, Juan Fº Madrid):
7. Main causes of infertility in males (Dr. F. Duncan)
8. Effect of esteroid and other esteroid disruptors in testicle function (Dr. L. Reynolds)
PAPER FOR ROUND TABLE DISCUSSION:
Harrison and Weiner, 1949. Vascular patterns of the mammalian testis and their functional significance.
MSc in Biology and Technology of Reproduction in Mammals
SUBJECT: MORPHOLOGY AND FUNCTION OF THE FEMALE REPRODUCTIVE ORGANS(Compulsory subject)
09:30 Lecture room. Introduction to the subject. Dr. Maria Jimenez 10:00 Theory. Lecture room Anatomy of the male and female reproductive system (human). Dr. K de Jong 12:00 Theor. Lecture room Comparative anatomy of reproductive organs in domestic mammals. Dr R. Latorre.
10:00 Theory. Lecture room Comparative anatomy of reproductive organs in domestic mammals Dr. R. Latorre Comparative anatomy of the mammary glands Dr. M.D. Ayala
9:30 Practice. Dissection room Comparative anatomy of reproductive organs in domestic mammals. Drs. R. Latorre & G. Ramírez
9:30 Theory. Medicine faculty Folliculogenesis, oogenesis and female gamete. Dr. Manuel Aviles
12:00 Theory. Lecture room Oocyte meiosis from basic science Dr. Francesca Duncan
Break Afternoon
15:30 Practice. Dissection room Anatomy of the female reproductive system (human). Dr. De Jong
16:00 Practice. Dissection room Female reproductive organs in canine Drs. MªD Ayala & G. Ramirez
15:30 Theory. Medicine faculty The ovary histological Organization Dr. Francesca Duncan
15:30 Practice. Medicine faculty Microscopic observation of ovary (newborn, Young, menopausal primate, mare). Dr. Concepción Ferrer
MSc in Biology and Technology of Reproduction in Mammals
9:30 Theory. Lecture room Histological organization of the reproductive tract. Dr. Larry Reynolds 12:00 Theory. Lecture room Origin and composition of the zona pellucida Dr. Maria José Izquierdo-Rico
9:30 Theory. Lecture room Physiology of female reproductive system. Dr. P Coy 11:30 Theory Estrous cycle in domestic females Dr. R Romar 13:00. Theory Control of estral cycle in domestical animals with special focus in cow. Dr. Dimitrios Rizos
10.00 Theory Lecture room Menstrual cycle in women. Control of menstrual cycle in women Dra. MT Prieto 13:00 Theory. Efectos no reproductivos de esteroides sexuales en la hembra. Dr. Aline Freitas
UNIVERSITY CLOSED
Break
Afternoon
15:30 Practice. Faculty of Medicine. Microscopy observation of uterus, oviduct, etc Dr. Juan Francisco Madrid Practice. Faculty of Medicine. Electron microscopy of the female reproductive organs. MJM SEMINAR SELECTION
15:30 Practice Lab Physiology Oestrus cycle in the bitch. Dr. Sebastian Cánovas
15.30 Practice Lab Physiology Identification of the different phases of the estrous cycle by observing the ovary Drs. R Romar and S. Cánovas
MSc in Biology and Technology of Reproduction in Mammals
SEMINARS 1. Composition of the uterine fluid. P Coy. 2. Functional relationship between the ovarian artery and vein: O. López Albors 3. Comparative anatomy of the female tract related to the artificial insemination: O. López Albors 4. Follicular atresia: M. Jimenez-Movilla 5. The polycystic ovarian syndrome in women: MJ Izquierdo, 6. Clinical cases related with the control of estrous cycle in pets. S. Cánovas 7. Hormonal regulation of the menstrual cycle and its relationship with contraceptives: R.Romar 8. Seasonal reproductive changes in mare and small ruminants. R Romar 9. Reproductive aging. Gamete quality and quantity. Dr. Franchesca Duncan 10. Steroids and endocrine disruptors in female reproductive functions. Dr L. Reynolds
PAPER FOR ROUND TABLE DISCUSSION: Monica M. Laronda, Alexandra L. Rutz, Shuo Xiao, Kelly A. Whelan, Francesca E. Duncan, Eric W. Roth, Teresa K. Woodruff & Ramille N. Shah. A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice. Nature Communications 8, Article number: 15261 (2017). doi:10.1038/ncomms15261
GAMETES-OVIDUCT INTERACTIONS BEFORE FERTILIZATION (1 week/3ECTS) (16-19th October 2016)
Coordinator: Pilar Coy Fuster
Theoretical Sessions: Room -1.3. Library (Veterinary Faculty). Practical Sessions: Department of Veterinary Physiology (1st floor, Faculty of Veterinary).
MONDAY16th Oct
TUESDAY17th Oct
WEDNESDAY18thOct
THURSDAY19thOct
FRIDAY20thOct
Morning
9:00: Theoretical session Presentation. Introduction to the Subject P Coy and R Romar 9:30 Theoretical session Generalities of the oviduct. Oviductal fluidcomposition. R Romar 11:30 Oocyte maturation during oviductal transport P Coy
9:00Practice II. Obtaining and storing oviductal fluid C. Soriano and R Romar 12:00Theoretical session Gene expression of the oviduct and protein composition M. Avilés
9:30 Practice IV. Preparation of oviductal fluid samples for enzymatic assays. R Romar 12:00 Theoretical session Interaction spermatozoa- oviduct. Sperm reservoir and capacitation in the oviduct R López-Úbeda.
9:00Practice III Sperm binding to oviductal epithelial cells. P Coy, C Soriano and S. Cánovas
9:00.Practice VI Assessment of the results ofthe enzymatic analysis in oviductal fluid R. Romar Discussion of scientific papers S Cánovas R. Romar
Break Afternoon
15:30 Practice I. Culture of oviductal epithelial cells. S. Cánovas and R.Romar
16:00 Practice V Changes in zona pellucida digestion mediated by the oviduct P Coy and S. Cánovas
Scientific papers proposed: CARRASCO LC, COY P, AVILÉS M, GADEA J, ROMAR R.Glycosidase determination in bovine oviduct fluid at
follicular and luteal phases of the estrous cycle. Reproduction, Fertility and Development20 1-10. 2008 CARRASCO LC, ROMAR R, AVILÉS M, GADEA J, COY P. Determination of glycosidase activity in porcine oviduct
fluid at the different phases of the estrous cycle. Reproduction136: 833–842. 2008.
SUBJECT: FERTILIZATION, EMBRYO DEVELOPMENT AND PREGNANCY(Compulsory subject)
From 30thOctoberth to 10thNovember 2017
Subject coordinated by P Coy Lecture room (theoretical sessions): room number-1.3 (located at ground floor of the library of the Veterinary Faculty).
Lab Veterinary Physiology: Department of Physiology, 1st floor Veterinary Faculty. Dissection room (Veterinary Anatomy).
1st week
MONDAY30 TUESDAY31 WEDNESDAY 1 THURSDAY2 FRIDAY3
Morning
9:00 h. Introduction to the subject S Cánovas 9:15 Theoretical session Fertilization. Gamete recognition María Jiménez 10:15 h Selection of seminars
9:00 Practice Obtaining of tubal oocytes Lab Histology Department Medical School 2nd floor. M. Avilés
9:00 Practice Obtaining of 2-cell embryos Lab Histology Department Medical School. 2nd floor. M. Avilés
12:00 h. Theoretical session Fertilization. Acrosomal reaction and sperm penetration MJ Izquierdo
12:00Theoretical session Role of zinc during fertilization and preimplantation embryo development Francesca Duncan
12:00 h Theoretical session Roles of oviductal proteins in mammalian fertilization and embryo development P Coy
Break
Afternoon
16:00 h Theoretical session Fertilization. Fusion of gametes and the polyspermy block M.Avilés
9:00 h Theoretical sessions Early stages of embryonic development (from zygote to blastocyst) F. Gil
9:30 h Theoretical session Implantation and placentation F. Gil 10:00 Practice: Dissection room Comparative anatomy of placentation (II) F. Gil G Ramírez R Latorre
9:00 h Practice: Dissection room Comparative anatomy of placentation (I) F. Gil O. López
9:30 h. Theoretical session Embryology of reproductive organs and mamary gland F Gil
9:30 Theoretical session Physiology of lactation in domestic animals S. Cánovas 11:30 Theoretical session Human lactation Veronica Muñoz Ricardo García de León
12:00 h Theoretical session Physiology of pregnancy in domestic animals Larry Reynolds
12:00 h Theoretical session Fetus development in humans. MT Prieto
Break
Afternoon
15:30 h Theoretical session Physiology of parturition Larry Reynolds
SEMINARIES 1. Effects of Maternal ‘Stressors’ on fetal and placental development, Larry Reynolds. 2. Sperm competition M. Avilés. 3. Species-specific gamete recognition. M. Avilés 4. Teratogens in women. MJ Izquierdo 5. Common malformations in the male reproductive organs. F Gil 6. Common malformations in the female reproductive organs.F Gil 7. Ectopic pregnancy in primates. P Coy 8. Parturition in the mare. R Romar 9. Practical information on lactation in human. R Romar 10. Comparative chronology of fetal development in human and domestic species. P Coy
PAPERS IN DISCUSSION
ZP2 peptide beads select human sperm in vitro, decoy mouse sperm in vivo, and provide reversible contraception. Avella MA, Baibakov BA, Jimenez-Movilla M, Sadusky AB, Dean J. Sci Transl Med. 2016 Apr 27;8(336):336ra60. doi: 10.1126/scitranslmed.aad 9946.
SPERM FUNCTIONAL ANALYSIS (2017-18)
MONDAY
13th November
TUESDAY
14th November
WEDNESDAY
15th November
THURSDAY
16th November
FRIDAY
17th November
9:30. Presentation
T. Sperm assessment.
Why, when and how?
Dr. J Gadea
P. Basic semen analysis
C. Soriano
J. Gadea
9:30 T. Introduction to
fluorescence techniques. Dr. J
Gadea
P. Epifluorescence microscope
Flow Cytometry
CASA
In 3 groups, C. Soriano, S.
Navarro, L. Vieira, J. Gadea
9:00 T. Sperm
capacitation.
T. Techniques to evaluate
sperm capacitation
Dr. R. López.
P. Inmunofluorescence
R. López, L. Vieira
P. Western blotting
L. Mtnez-Fresneda
9:00. Sperm selection by
magnetic beads
Dr. Juan Carlos Martínez
Soto. IVI Murcia.
T. Prediction of fertility
(Dr. Gadea)
9:30 T. Sperm assessment
by in vitro techniques (Dr.
Gadea)
Calcium measurements
Fluorometry and flow
cytometry measurements
L Vieira/S. Navarro
lunch lunch lunch lunch lunch
18:00 PhD Thesis Silvia
Abril
Alternative semen
collection methods to
improve animal welfare
and seminal quality in
small ruminants
T. 16:00 Seminal analysis in
human clinic
Dr. E. Sellés. IVI Alicante
T. Sperm DNA
fragmentation. F Staicu
P. Sperm DNA
fragmentation (SCSA and
Halomax)
F. Staicu, S. Navarro, J.
Gadea
MONDAY
20th November
TUESDAY
21th November
WEDNESDAY
22th November
THURSDAY
23th November
FRIDAY
24th November
12:00
T. Spermatic tropism
(quimiotaxis, termotaxis
and rheotaxis) (Dr.
Perez-Cerezales).
T. Assessment of DNA
damage in spermatozoa
(Dr. Perez-Cerezales).
AEAV evaluation visit 9:30
T & P. Sperm preparation
for IVF
C. Soriano
12:00. Sperm motility
(Dr. Carles Soler. Univ.
Valencia)
IVM, IVF & ET
lunch lunch lunch Lunch lunch
Journal Club
(Dra. María Jimenez, UM)
T. Computer assisted
sperm analysis (CASA)
(Dr. Carles Soler. Univ.
Valencia)
IVM, IVF & ET
Falta incluir: Presentación de asignaturas optativas (mañanas del lunes, martes o jueves?)
SUBJECT “IN VITRO MATURATION, IN VITRO FERTILIZATION AND EMBRYO CULTURE”. 27th November-1st December and 11th-15th December. 2017
Master Course “Biology and Technology of Reproduction in Mammals”. 2017-18.
Theoretical Sessions: Room -1.2. Library (Veterinary Faculty). Laboratory Sessions: Department of Veterinary Physiology (1st floor, Faculty of Veterinary).
MONDAY 20 Nov TUESDAY 21 Nov WEDNESDAY 22 Nov THURSDAY 23 Nov FRIDAY 24 Nov
9.00-9.30h. Subject Presentation. Groups arrangement. R. Romar Five A-groups: 4 students/group Seven B-groups: 3 students/group Three C-groups: 7 students/group 9.30-11.00h Lesson 1. Composition and preparation of culture media. S. Cánovas 11.30h. Practice 1. Groups A1 (11.30h), A2 (12.15h) and A3 (13.00h) Preparation of culture media for manipulation of cumulus oocyte complexes (COCs). JA. Carvajal
15.00h. Practice 1 (cont.). Groups A4 (15.00h) and A5 (15.45h). Preparation of culture media for manipulation of (COCs). R. Romar
SUBJECT “IN VITRO MATURATION, IN VITRO FERTILIZATION AND EMBRYO CULTURE”. 27th November-1st December and 11th-15th December. 2017
Master Course “Biology and Technology of Reproduction in Mammals”. 2017-18.
Theoretical Sessions: Room -1.2. Library (Veterinary Faculty). Laboratory Sessions: Department of Veterinary Physiology (1st floor, Faculty of Veterinary).
MONDAY 27 Nov TUESDAY 28 Nov WEDNESDAY 29 Nov THURSDAY 30 Nov FRIDAY 1 Dec 9.00-10.15h. Lesson 2. Oocyte in vitro maturation (IVM) in domestic species: generalities, current status and results. R. Romar 10.30-11.45h. Lesson 3. Oocyte meiosis from basic science to clinical consequences I F. Duncan 12.00-13.45h. Lesson 4. Oocyte meiosis from basic science to clinical consequences II F. Duncan
9.00-11.00h. Practice 3. (All groups). Fixing and staining of oocytes. R. Romar and F. Duncan 11.15-12.30h. Lesson 5. IVM results evaluation (slides). R. Romar and F. Duncan 12.45-14.00h. Practice 4. Groups B7 and B6. Evaluation of nuclear maturation (fluorescence microscope). P. Coy and F. Duncan
9.30-10.45h. Lesson 6. In vitro fertilization I (IVF): generalities and current status. R. Romar 11.00-12.15h. Lesson 7. In vitro fertilization II (IVF): evaluation of results after IVF. R. Romar 12.30-13.45h. Practice 4 (cont.). Groups B3 and B2. Evaluation of nuclear maturation (fluorescence microscope). S. Cánovas and C. Soriano
9.30-10.45h. Practice 5. Group C1. Porcine IVF. R. Romar and E. Paris 11.00-12.15h. Practice 5 (cont.). Group C2. Porcine IVF. R. Romar and E. Paris 12.30-13.45h. Practice 5 (cont.). Group C3. Porcine IVF. R. Romar and E. Paris
9.30-10.45h. Practice 6. (cont.). Groups B1 and B2. Evaluation of fertilization results (fluorescence microscope). E. Paris and S. Cánovas 11.00-12.15h. Practice 6 (cont.). Groups B3 and B4. Evaluation of fertilization results (fluorescence microscope). P. Coy and C. Soriano
15.30-18.30h. Practice 2. (All groups). Obtaining, manipulation and in vitro maturation of porcine cumulus oocyte complexes. S. Cánovas and E. Paris
15.00-16.15h. Practice 4 (cont.). Groups B5 and B4. Evaluation of nuclear maturation (fluorescence microscope). P. Coy and S. Cánovas
15.00-16.15h. Practice 4 (cont.). Group B1. Evaluation of nuclear maturation (fluorescence microscope). C. Soriano
15.30-16.45h. Practice 6 Groups B7. Evaluation of fertilization results (fluorescence microscope). S. Cánovas
SUBJECT “IN VITRO MATURATION, IN VITRO FERTILIZATION AND EMBRYO CULTURE”. 27th November-1st December and 11th-15th December. 2017
Master Course “Biology and Technology of Reproduction in Mammals”. 2017-18.
Theoretical Sessions: Room -1.2. Library (Veterinary Faculty). Laboratory Sessions: Department of Veterinary Physiology (1st floor, Faculty of Veterinary).
MONDAY 11 Dec TUESDAY 12 Dec WEDNESDAY 13 Dec THURSDAY 14 Dec FRIDAY 15 Dec
11.00-12.15h. Practice 6 (cont.). Groups B5 and B6. Evaluation of fertilization results (fluorescence microscope). P. Coy and F. Duncan 12.30-14.00h. Lesson 8. Embryo culture (EC I): generalities and problems derived from in vitro culture. D. Rizos, INIA
9.00-10.30h. Lesson 9. Embryo culture and development in humans. V. Hurtado de Mendoza (ASEBIR) 11.00-12.30h. Lesson 10. Embryo classification in humans. V. Hurtado de Mendoza
9.30-10.45h. Lesson 11. Intracitoplasmic sperm injection (ICSI). E. García-Roselló (UCH-CEU Valencia) 11.00-12.15h. Practice 8. Group B7. ICSI of pig oocytes. FA. García and E. García-Roselló 12.30-13.45h. Practice 8 (cont.). Group B6. ICSI of pig oocytes. FA. García and E. García-Roselló
9.30-10.45h. Practice 8 (cont.). Group B4. ICSI of pig oocytes. FA. García and E. García-Roselló 11.00-12.15h. Practice 8 (cont). Group B3. ICSI of pig oocytes. FA. García and E. García-Roselló 12.30-13.45h. Practice 8 (cont.). Group B2. ICSI of pig oocytes. FA. García and E. García-Roselló
10.00h. Mock exam. R. Romar
15.00-17.30h. Practice 7. All groups. Evaluation of embryo development in pig and cow (stereomicroscopes and fluorescence microscopes). P. Coy, R. Romar and E. Paris
15.30-16.45h. Practice 8 (cont.). Group B5. ICSI of pig oocytes. FA. García and E. García-Roselló
15.30-16.45h. Practice 8 (cont.). Group B1. ICSI of pig oocytes. FA. García and E. García-Roselló
CRYOPRESERVATION of GAMETES and EMBRYOS (February 12 - 16, 2018)
MONDAY
February 12
TUESDAY
February 13
WEDNESDAY February 14
THURSDAY February 15
FRIDAY
February 16
Theory I (9.30-14.30 h)
Introduction. Practice Groups Distribution
Basics of Cryobiology
Sperm Cryopreservation
Salvador Ruiz (UMU)
Oxidative Stress and Cryopreservation
Frozen Semen and Fertility
Joaquín Gadea (UMU)
Master Classroom Veterinary Faculty
Practice I (9-11.45 h)
G. A: Freezing Boar Semen
G. B: Freezing Bovine Semen
G. C: Frozen/Thawed Semen Evaluation
S. Ruiz, L. Vieira, J. Gadea
Practice II (12-14.45 h)
G. B: Boar Semen
G. C: Bovine Semen
G. A: Semen Evaluation
S. Ruiz, L. Vieira, J. Gadea
Physiology Lab
Theory III (11.30-14.30 h)
Freezing Horse Semen
Jordi Miró (Univ. Autónoma Barcelona)
Master Classroom Veterinary Faculty
Practice V (9.30-11.30 h)
Obtaining Oocytes for Vitrification
(Complete Group)
Cristina Soriano L. Vieira (UMU)
Physiology Lab
Theory IV (12-14 h)
Cryopreservation of Gametes and Embryos in Lab Animals.
Basics and Applications
Gonzalo Moreno Del Val (Instituto Neurociencias Alicante)
Master Classroom Veterinary Faculty
Theory & Practice II
(9.15-11.45 h)
Freezing Human Semen
(Complete Group)
Juan Carlos Martínez (IVI-Murcia)
Physiology Lab
Theory V (12-14 h)
Cryopreservation of Pluripotent and
Induced Embryonic Stem Cells
Sebastián Cánovas (UMU)
Master Classroom Veterinary Faculty
Theory II (16-18 h)
Factors affecting Freezing Semen
Freezing Protocols
Luis Vieira (UMU)
Master Classroom Veterinary Faculty
Practice III (16-18.45 h)
G. C: Boar Semen
G. A: Bovine Semen
G. B: Semen Evaluation
S. Ruiz, L. Vieira, J. Gadea
Physiology Lab
Practice IV (16.30-19.30 h)
Freezing Horse Semen
(Complete Group)
J. Miró R. Diego, S Ruiz
Physiology Lab
Theory & Practice I (16-20 h)
Vitrification of Oocytes and Embryos
(Complete Group)
Beatriz Amorocho David Gumbao
(IVI-Murcia)
Physiology Lab
18 Students (Group A: 6 students, Group B: 6, Group C: 6).
EPIGENETICS IN REPRODUCTIVE BIOLOGY 26 FEBRUARY-2 MARCH 2018
MONDAY 26 February TUESDAY 27 February
WEDNESDAY 28 February
THURSDAY 1 March FRIDAY 2 March
9:30 Welcome and introduction to the course. “Los nuevos secretos de nuestra herencia” https://www.youtube.com/ watch?v=dLKl6qJhPM8 Pilar Coy
9:30 Practical session (laptop required) Bisulfite sequencing and SeqMonk tools for BS methylation analysis./ Taller práctico (se require protátil). Secueciación de bisulfito y herramientas en SeqMOnk para el análisis de metilación de bisulfito. Pilar Coy
9:30 Impact of epigenetics on embryonic development. /Impacto de la epigenética en el desarrollo embrionario Alfonso Gutierrez Adan (INIA, Madrid)
10:00 Methods for profiling DNA methylation and transcription in single cell or reduced cell number/ Técnicas para caracterizar la metilación del ADN y transcripción en células individuales o número reducido de células
Antonio Galvao (Babraham Institute, Cambridge, UK)
10:00 "Understanding the placenta in ageing and disease"/Entendiendo la placenta durante el envejecimiento y la enfermedad Vicente Perez-García (Babraham Institute, Cambridge, UK)
10.30 What is epigenetics? And How can epigenetics be studied? /¿Qué es la epigenética y como puede ser estudiada? Ann Van Soon (Univ. Gent)
12:00 Impact of Assisted Reproductive Technologies on DNA methylation during preimplantation development. /Impacto de las técnicas de reproducción asistida sobre la metilación del ADN durante el desarrollo preimplantacional.
12:30. Epigenetic reprogramming mechanisms in early embryo development. /Mecanismos de reprogramación epigenética durante el desarrollo
12:00 Practical session (laptop required) Analysis of DNA methylation profile in gene promoters Taller práctico (se requiere portátil). Análisis del perfil de
12:00 How does metabolism affect the epigenome of our gametes. /¿Como afecta el metabolismo el epigenoma de los gametos? Antonio Galvao (Babraham Institute, Cambridge, UK)
12:00 Discussion of paper and presentations of seminar topics. /Discusión del artículo científico y presentación de los temas de seminarios. Sebastián Cánovas/Pilar Coy
EPIGENETICS IN REPRODUCTIVE BIOLOGY 26 FEBRUARY-2 MARCH 2018
Evaluación:
1. Examen preguntas tipo test y cortas sobre los conceptos básicos de la asignatura (40%). 2. Presentación y discusión de trabajos (40%). Los alumnos en parejas (2 personas) elaborarán un documento (trabajo dirigido) sobre un tema
relacionado con el contenido de la asignatura, entre los propuestos por el profesorado. Además participarán en la discusión de un artículo científico
propuesto por el profesorado.
3. Trabajo del estudiante (20%): asistencia, implicación con el grupo y participación
Selection of paper and seminar topics. /Selección del artículo científico para discusión y de los temas de seminarios Pilar Coy
embrionario temprano. Sebastian Cánovas
metiulación del ADN en los promotores. Sebastián Cánovas
Lunch Lunch Lunch Lunch Lunch
16:00 Epigenetic re-programming through the germ cell development and gametogenesis Sebastian Cánovas
ASIGNATURA “Transgénesis, terapia génica, clonación y células madre”.Del 5 al 9 de Marzo de 2018. Máster “Biología y tecnología de la reproducción en mamíferos”. Curso 2017-18.
Sesiones teóricas: Clase -1.2, Biblioteca. Sesiones prácticas: Departamento de Fisiología. Facultad Veterinaria.
LUNES 5 marzo MARTES 6 marzo MIERCOLES 7 marzo JUEVES 8 marzo VIERNES 9 marzo
9:30. Presentación del curso yguía docente
Interés de los animalestransgénicos en la agricultura y lamedicina.
Aspectos éticos y morales de latransgénesis animal
Producción de cerdostransgénicos. Metodología yaplicaciones
Dr. Joaquín Gadea. UM
9:30. Bases moleculares de latransgénesis. Diseño detransgenes.
Uso de endonucleasas derestricción para generaranimales tránsgénicos
CRISPR-Cas y su aplicación enanimales de granja
Prácticas: diseño de Crispr-cas9
Dr. Pablo Bermejo-ÁlvarezINIA Madrid.
9:30. Producción debiomateriales. Transgénesis en elgusano de sedaDr. Salvador Aznar. IMIDA
12:00. Clonación: transferencianuclear en bovino. Dr. SebastiánCánovas. UM.
9:30. Cerdos transgénicos paraxeno-trasplantes
Dr. Guillermo Ramis. UM
12.00. Células madre: ¿qué son?,tipos, ¿Cómo se obtienen y cómose cultivan? Aplicaciones.Reprogramación celular ytransdiferenciación. Dr. SebastiánCánovas. UM.
10:00. Uso de células madremesenquimales con utilidad enmedicina regenerativa deenfermedades prevalentes.
Dr. Juan Antonio Marchal. UGR
12:00. Células madrecancerígenas: biomarcadoresdiagnósticos y terapias dirigidas.Dr. Juan Antonio Marchal. UGR
16:00. Utilización de losespermatozoides como vectoresde la transgénesis: SMGT.
16:00. Prácticas de transfecciónde células. Dra. M. JimenezMovilla. Julieta Hamze. UMU.
Preparación del trabajo derevisión. Dr. Joaquín Gadea. UM
Activación de ovocitos paraobtener partenotes. SergioNavarro. UMU.
16.00. Terapia celular en animalesdomésticos. Dr. Jesús Talavera.UM.
Inyección de ovocitos-embrionescon DNA-Crispr-cas9. SergioNavarro. UMU
Evaluación: Examen preguntas cortas sobre los conceptos básicos de la asignatura.Los alumnos en parejas (2 personas) elaborarán un documento de revisión sobre un tema relacionado con la transgénesis animal, clonación, terapia génica océlulas madre. Este documento tendrá un formato de revista científica que si tiene la calidad suficiente y así se desea podrá ser enviada para su evaluación. Fechade entrega documento: lunes 7 de mayo. Presentación: 10 min día del examen. Exámenes: 8 Junio 2018 y 13 Julio 2018
Monday 12 March Tuesday 13 March Wednesday 14 March Thursday 15 March Friday 16 March
10.00-13.30h
Presentation
Lesson 1.
Current state of thereproductive technologies
Dr. Joaquín GadeaDpto. Fisiología, UMU
Lesson 2.
Superovulation in equids and aplications
Dr. Ignacio Giménez Nebot, Rara Avis Biotecnología
Lesson 4.
Breeding and care of Rhinoceros
Dña. Fabiola QuesadaDirectora Wild Spirit
Lesson 5.
Assisted vs. Natural reproduction in African wild species
Dña. Fabiola QuesadaDirectora Wild Spirit
Lesson 6. 12.30h
Oestrus siychronization, artificial insemination and embryotransfer in bovine
Dra. Susana Astiz, INIA
Lesson 7.
Sex sorted semen
Dr. Francisco García-VázquezDpto. Fisiología, UMU
Lesson 8.
Reproductive biotechnologies in porcine: embryo transfer
Dr. Francisco García-VázquezDpto. Fisiología, UMU
Lesson 9.Physiology of reproduction indolphins and appliedbiotechnology
Dra. María Jesús SánchezCalabuig, INIA
Lesson 10. Porcine Artificial Inseminationcenters
D. Pedro José LlamasImport –Vet
Lesson 11.Artiificial insemination in porcineD. Pedro José LlamasImport –Vet
Lesson 12.
Artificial ovary and theapplication in endangeredrecovery species
Dr. Luis VieiraDpto. Fisiología, UMU
Lesson 13.African Elephant Inmuno-contraception
Dra. Pilar CoyDpto. Fisiología, UMU
Lesson 14.Interspecies Nuclear Transfer:uses and limitations forrecovering endangered species
Dr. Sebastián Cánovas,Dpto. Fisiología, UMU
14.00-16.00h
16.00-19.00h
Lesson 3.
Artificial insemination and embryo transfer in equids
D. Josep Giner Torres,Equus Ibérica
Practice 1.
Artificial insemination in bovine
Dra. Susana Astiz, INIA Dr. Salvador Ruiz, Dpto. Fisiología, UMU
UMU farm
Practice 2.
Artificial insemination in porcine
Dr. Francisco García-Vázquez,D. Pedro José Llamas LópezDpto. Fisiología, UMU
Agropor Company
SUBJECT “REPRODUCTIVE BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES”. March 12-16th.
Master Course “Biology and Technology of Reproduction in Mammals”. 2017-18.
Applications of Ultrasonography in Reproductive Biology (April 9 - 13, 2018)
