Eng. SIMONA-CLARA BÂRSAN
SUMMARY OF THE PhD THESIS
RESEARCH REGARDING THE INFLUENCE OF IRRIGATION REGI MEN,
FERTILIZATION AND BIOLOGICAL MATERIAL UPON
THE PRODUCTION OF ROOTS AND BIOETHANOL OBTAINED
FROM SUGAR BEET GROWN IN THE TRANSYLVANIAN PLAIN
SCIENTIFIC COORDINATOR
Prof. univ. EMIL LUCA
CLUJ-NAPOCA
2012
UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE
CLUJ-NAPOCA
FACULTY OF AGRICULTURE
DOCTORAL SCHOOL
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
2
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
3
SUMMARY
pag.
FOREWORD …………………………..………………………………………………....…. 5
INTRODUCTION …………………………….................................................................…… 7
CHAPTER I . CURRENT STATUS OF RESEARCH ON SUGAR BEET CROP……... 7
CHAPTER II. IRRIGATION REGIMEN AND WATER CONSUMPTION OF
SUGAR BEET CROP................................................................................................................
8
2.2. IRRIGATION REGIMEN….............................................................................. 8
CHAPTER III. PRESENT STAGE OF RESEARCH REGARDING BIOETHANOL
OBTAINED FROM SUGAR BEET .........................................................................................
9
CHAPTER IV. RESEARCH OBJECTIVES ........................................................................ 10
4.1. GENERAL OBJECTIVES................................................................................. 10
4.2. SPECIFIC OBJECTIVES.................................................................................. 11
CHAPTER V. EXPERIMENTAL AREA DESCRIPTION ................................................ 11
CHAPTER VI. BIOLOGICAL MATERIAL AND RESEARCH METHODS ……….… 12
6.2. RESEARCH METHODS AND APPLIED CROP TECHNOLOGY................ 13
6.2.1. Location and organization of experiences....................................... 13
6.2.2. Crop technology applied in the experimental field from
Viişoara–Turda, during 2008-2010..................................................
13
CHAPTER VII. RESULTS OF THE RESEARCH REGARDING THE INFLUENCE OF
IRRIGATION REGIMEN, FERTILIZATION AND BIOLOGICAL MA TERIAL UPON
THE YIELD OF SUGAR BEET GROWN IN THE TRANSYLVANIAN
PLAIN …………………………………………..…………………………………………………
13
CHAPTER VIII. RESULTS OF THE RESEARCH REGARDING THE INFLUENCE
OF IRRIGATION REGIMEN, FERTILIZATION AND BIOLOGICAL MATERIAL
UPON THE PRODUCTION OF BIOETHANOL OBTAINED FROM SUG AR BEET
GROWN IN THE TRANSYLVANIAN PLAIN ........................................................................
15
CHAPTER IX. RESULTS OF THE RESEARCH REGARDING THE
DETERMINATION OF WATER CONSUMPTION AND IRRIGATION
EFFICIENCY OF THE SUGAR BEET GROWN IN THE EXPERIMEN TAL FIELD
FROM VII ŞOARA - TURDA .............................................................................................
17
9.1. RESULTS OF THE RESEARCH REGARDING THE DETERMINATION
OF WATER CONSUMPTION OF THE SUGAR BEET CROP,
VIIŞOARA–TURDA, 2008...............................................................................
17
9.2. RESULTS OF THE RESEARCH REGARDING THE DETERMINATION
OF WATER CONSUMPTION OF THE SUGAR BEET CROP,
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
4
VIIŞOARA–TURDA, 2009............................................................................... 19
9.3. RESULTS OF THE RESEARCH REGARDING THE DETERMINATION
OF WATER CONSUMPTION OF THE SUGAR BEET CROP,
VIIŞOARA–TURDA, 2010...............................................................................
20
9.4. DETERMINATION OF WATER USE COEFFICIENT IN CROP
IRRIGATED CONDITIONS, VIIŞOARA–TURDA, 2008-2010.............…....
21
9.5. DETERMINATION OF WATER USE EFFICIENCY FOR THE SUGAR
BEET CROP GROWN IN IRRIGATED CONDITIONS,
VIIŞOARA–TURDA, 2008-2010 .....................................................................
21
CHAPTER X. RESULTS OF THE RESEARCH REGARDING THE ECONOMIC
EFFICIENCY OF THE IRRIGATED SUGAR BEET CROP IN THE CONDITIONS
OF THE TRANSYLVANIAN PLAIN ………………..……………..………………………
22
CHAPTER XI. RESULTS OF THE RESEARCH REGARDING THE QUALITY OF
THE BIOETHANOL OBTAINED FROM SUGAR BEET GROWN AT
VII ŞOARA-TURDA ........................................................................................................
24
CHAPTER XII. GENERAL CONCLUSIONS ..................................................................... 25
12.1. CONCLUSIONS REGARDING THE INFLUENCE OF IRRIGATION
REGIMEN, FERTILIZATION AND BIOLOGICAL MATERIAL UPON
THE PRODUCTION OF ROOTS AND BIOETHANOL OBTAINED
FROM SUGAR BEET GROWN IN THE TRANSYLVANIAN PLAIN ……
25
12.2. CONCLUSIONS REGARDING THE ECONOMIC EFFICIENCY OF
IRRIGATION ON THE YIELD OF SUGAR BEET GROWN IN THE
CONDITIONS OF VIIŞOARA-TURDA, 2008-2010 ……..………………....
27
12.3. CONCLUSIONS REGARDING THE QUALITY OF BIOETHANOL
OBTAINED FROM SUGAR BEET GROWN IN THE CONDITIONS OF
VIIŞOARA-TURDA ……......…………………………..………..……...........
28
SELECTIVE BIBLIOGRAPHY .............................................................................................. 29
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
5
Key words: sugar beet, irrigation regime, fertilization, biological material (variety),
Transylvanian Plain, bioethanol
FOREWORD
Currently, the cultivation of sugar beet in Romania is carried out only for the purpose of
obtaining sugar, whose production quota is established by regulations of the European Commission
and which can be obtained by growing, in normal thermal and rainfall regimens, a share of only
10% out of the approximately 250,000 hectares representing the agricultural land suitable for this
crop.
In this respect, the present PhD thesis aims to bring viable and feasible arguments, on the
one hand, in advantage of practicing irrigations for the sugar beet culture in the Transylvanian
Plan, for the purpose of achieving an efficient economic production, especially in years with
defficient rainfall regimens and, on the other hand, to obtain a superiour valuation of the sugar
beet productions by producing bioethanol.
The experiences carried out during the elaboration of the thesis with the title “RESEARCH
REGARDING THE INFLUENCE OF IRRIGATION REGIMEN, FERTILIZATION AND
BIOLOGICAL MATERIAL UPON THE PRODUCTION OF ROOTS AND BIOETHANOL
OBTAINED FROM SUGAR BEEET CULTIVATED IN THE TRANSYLVANIAN PLAIN” were
developed during 2008-2010 on a field located on the experimental extravilanarea of the Viişoara
village, Cluj county, on the left bank of Arieş river. The village is situated in the South-Eastern part
of Cluj county, about 2 km away from the town of Câmpia-Turzii, near the European route E60.
The experimental field is part of the arable land belonging to SC NORA LY AGROSERV SRL,
located in Viişoara village.
The PhD thesis is structured in 12 chapters together with an Annex with experimental data
and is presented on 393 pages, containing 157 tables and 93 figures, with 174 bibliographic
references.
My first thought of thanks and gratitude is turning to Mr. Prof. PhD eng. Emil Luca,
prestigious scientific personality in the field of irrigation technologies, particularly for the guidance
and support he gave me as a scientific coordinator, both during the carried out experiments and
elaboration of the thesis.
I also wish to bring special thanks to Mr. eng. Gheorghe Marinca, administrator of SC NORA
LY AGROSERV SRL, for the openness and kindness he put on my disposition the agricultural field
necessary for carrying out the experiences necessary for the reasearch included in my PhD thesis,
as well as for his very competent advice related to practical aspects of sugar beet cultivation under
the conditions from Viişoara - Turda.
It is no doubt that the fermentation process of the experimental samples would not have been
possible without the support and scientific infrastructure that I received or was put at my disposal
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
6
during the three years of experimental research from USAMV Cluj-Napoca, Department of Soil
Science and Technical Sciences, in particular from Mrs. Lecturer Adriana David, in which
I discovered not only a perfect specialist in the field of molasses fermentation, but also a true
friend, whom I thank with all my honesty.
On this way, I also wish to express my sincere thanks to my colleagues from ICIA
Cluj-Napoca, PhD Adriana Gog, PhD Oana Cadar, sing. Maria Sarca, sing. Ludovic Ferenczi and
PhD student Lăcrimioara Şenilă, especially for the competence and professionalism they assisted
me with for the determination of the bioethanol distillation method and of the bioethanol
physico-chemical and energetic characteristics. I sincerely appreciate the support and
understanding of ICIA Cluj-Napoca leadership, particularly of Mr. Director Dr. eng. Mircea
Chintoanu, who supported and encouraged me throughout the preparation of the thesis.
Finally, I want to thank to my family and friends, for the moral support and patience with
which they surrounded me during the completion of this thesis.
The Author
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
7
INTRODUCTION
Out of the total current arable area of Romania, of approximately 8.2 million ha, the cultivable
sugar beet area is about 250,000 ha, this plant being very demanding towards the ground, all its
properties influencing the production level and technological quality indices. Currently, in
Romania, at an estimated annual consumption of about 5,000,000 t of gasoline, it takes about
287,500,000 l of bioethanol per year in order to reach the required content of 5.75% bioethanol into
gasoline, and this quantity is meant to grow until 2020, as consequence of the increase, up to 10%,
of the bioethanol content, according to European norms.
In the meantime, the cultivation of sugar beet for producing bioethanol will have a series of
positive effects, among which: improvement of crop rotation on about 850,000 ha, by applying a
four year rotation; increase of the farmers’ income and creation of new jobs, not only in the sugar
beet - growing farms, but also in the ethanol distilleries that will be set up; development of the
livestock farms, using as animal feed or as a source of biogas the by-product resulted from the
ethanol distillation. Thus, at an average production of only 50 t/ha, the total production of sugar
beet would be of 11,000,000 t, from which about 1,170,000 t of ethanol would result, thus covering
the bioethanol requirements of Romania.
Presently, in Romania, the number of existing plants for the production of bioethanol is
reduced and they use as raw material only corn, in particular due to the fact that the areas in which
they have been built are supportive of this crop. In 2009, in Zimnicea was set up the largest refinery
of bioethanol, with a production of about 100,000 t/year. In the following period, a bioethanol
factory using sugar beet as raw material will be built near Braşov. In this way, one will ensure the
purchase of larger amounts of the sugar beet from farmers, in circumstances where the production
of the Romanian sugar quota will remain unchanged. To limit the predicted increase in the global
temperature and greenhouse gas emissions, Romania will act, in particular, in the field of energy
efficiency increase, as well as in the field of increasing the use of renewable sources of energy,
having the following effects: reduction of adverse environmental impacts; increase of the energy
supply security; recovery of the existing agricultural potential by encouraging plant crops, such as
sugar beet, used as raw materials for the production of bioethanol.
CHAPTER I
CURRENT STATUS OF RESEARCH ON SUGAR BEET CROP
Originating in Central Asia and peripheral Mediterranean areas, the sugar beet cultivation has
been known since ancient times. After the conquest of Egypt, Syria and Babylon, the Arabs brought
the beet in the Orient, wherefrom it was later widespread in Asia, India, China and Japan
(PASTOR, 2002). The first scientific description of beet appeared in the 16th century, when, in the
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
8
book entitled "De Plantis”, Caesolpin presented the 4 species of beet known at that time, namely:
white beet, noble beet, branched root beet and red beet (LAMARCK, 1783; STĂNESCU, 1974).
Sugar beet culture was introduced into Europe in the early 19th century after scheduling the
continental blockade by Napoleon, who had forbidden the import of sugar obtained from sugar cane
on the European continent. Consequently, the first factory in Europe and in the world to produce
beet sugar was built in Silesia in 1802.
The first 300 kg of crystallized sugar were obtained by Franz Karl Achard (1753-1821)
(GHERMAN, National Institute of Research, Development for Potato and Sugar Beet Braşov). In
view of the evolution and expansion of beet crops, Napoleon ordered the cultivation of fodder beet
as feedstock for sugar extraction, both in France and in the occupied territories, which, by default,
led to the building and development of several sugar factories.
CHAPTER II
IRRIGATION REGIMEN AND WATER CONSUMPTION OF SUGAR B EET CROP
The increase of the efficiency of crops irrigation, for refining the productive potential of soil
and plants, is aimed at solving the technological problems related to structure and crop rotation,
soil, fertilization, water consumption and irrigation technique. It is well known the fact that,
together with the global warming, drought has affected almost 50% of the agricultural areas.
Therefore, crops irrigation comes as a necessity in the steppe, forest-steppe and humid areas of the
former oak forests, in the plain regions as well as in areas where rainfall is abundant (BUDIU,
1992; NAGY, 1994). An important role in establishing an efficient irrigation system is represented
by the used and developed variety of sugar beet culture technology and, last but not least, by the
used watering methods.
The most widespread irrigation method is surface leaking. The method is relatively cheap,
requiring lower energy consumption in the distribution of water in plants and presenting many
variations of the water distribution method, such as furrow or stripe irrigation. Furrow irrigation is
used in watering facilities for vegetable production in perennial crops, horticulture and viticulture,
on land with uniform slopes. The achievement of a correct irrigation system involves the following
stages: land levelling; furrow opening; watering equipment installation; watering scheduling;
watering elements calculation. Stripe watering is used for cereal straw and perennial legumes on
lands with low and moderate slope surface (up to 2%), on less permeable soils, with average or
average to heavy textures (LUCA, 2008).
2.2. IRRIGATION REGIMEN
Crops irrigation provides at least 13% of the optimal water requirements of the soil, which
requires as appropriate the expansion of areas and irrigation equippment. The relationship between
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
9
production and water consumption highlights the strict reliance between the reaching of the plant
production potential and water consumption (NICOLESCU 2002). In establishing the irrigation
regimen, in addition to the water needs of plants in different vegetation stages, roots depth
development, physical properties, chemical and hydrophysics indices of soil or climatic conditions,
it is also necessary to know the minimum limit under which humidity must not drop, as well as the
soil water reserve at the beginning and the end of the vegetation period, the groundwater depth and
its content of salts (FLORESCU, 1967).
The components of the irrigation system are represented by: � irrigation norm; � watering
norm; � watering application time; � number and duration of waterings.
The irrigation norm depends on the water balance and is determined according to the
vegetation period of plants (for the entire vegetation period or even on different phenophases).
Having regard to the fact that the irrigation norm depends on the water requirements of plants, on
the amount of water in the soil at one given moment (at sowing) and on the water resulted from
precipitations and groundwater aquifers, it consequently results that its size is not a fixed element,
its variation depending on the above mentioned parameters (LUCA şi NAGY, 1999; LUCA,
BUDIU, Ana CIOTLĂUŞ, 2008). The size of the irrigation norm is positively or negatively
influenced by the culture, crop technology and the productive potential of the biological material
that is used (LUCA, 2008). The irrigation norm can be calculated using the equation of water
balance from soil, as it follows:
� for soils with closed balance:
∑m = ∑(e + t) + Rf + Ri - Pv
� for soils with open banace:
∑m = ∑(e + t) + Rf + CC – Pv - Af
where:
∑m = irrigation norm (m³/ha); ∑(e+t) = total water consumption (m³/ha);
Rf = final water supply (m³/ha); Ri = initial watter supply (m³/ha);
Pv = summer precipitations (m³/ha); CC = field capacity (m³/ha);
Af = phreatic supply (m³/ha).
CHAPTER III
PRESENT STAGE OF RESEARCH REGARDING BIOETHANOL
OBTAINED FROM SUGAR BEET
Concerned about the continuous growing global pollution, the 160 countries that signed the
Kyoto Protocol undertook to reduce the greenhouse gas emissions by 5.20%, during 2008–2012,
down to the 1990 level. The ethanol economy could follow the economy of hydrocarbons, gas and
methanol synthesis, without major events and maintenance of almost all of superstructures of the
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
10
sector for the production and marketing of fuels and petrochemical raw materials. The switch to this
type of economy requires an appropriate development level of biotechnologies, particularly in the
agricultural sector. The research carried out in recent years has shown that the solar energy stored in
biomass may constitute a source of clean and renewable energy, thus representing a viable
alternative to fossil fuels.
The use of ethanol, pure (E100) or blended with fossil fuels in different proportions is the
cheapest and ecological way to reduce gasoline consumption and to diminish atmosphere pollution.
By burning bioethanol, the carbon dioxide, water and solar energy accumulated by biomass through
the process of photosynthesis are released back in the atmosphere, in this way the bioethanol
contribution to global warming through the greenhouse effect being zero. In the same positive
sense, one should mention the antipollution laws, which severely restrict the amount of noxes from
the exhaustion gas of the internal combustion engines (TRIPŞA, 2006). Furthermore, some
countries of EU have created a series of policies and regulations that encourage the development of
biofuel field (reductions in excise duties on fuels, credits granted to farmers for biomass, etc.).
CHAPTER IV
RESEARCH OBJECTIVES
4.1. GENERAL OBJECTIVES
The experimental research carried out during 2008-2010 in the conditions from
Vii şoara-Turda aimed at establishing the influence of irrigation, fertilization and variety on the
production of roots and bioethanol obtained from sugar beet grown in the Transylvanian Plain.
In this way, the experiences associated with the thesis having the title "RESEARCH
REGARDING THE INFLUENCE OF IRRIGATION REGIMEN, FERTILIZATION AND
BIOLOGICAL MATERIAL UPON THE PRODUCTION OF ROOTS AND BIOETHANOL
OBTAINED FROM SUGAR BEET GROWN IN THE TRANSYLVANIAN PLAIN" come to
reinforce the need for studying some norms of the irrigated crop technology for sugar beet varieties
in Transylvania, plus the analysis of the influence of the fertilization factor upon the final product
quality and quantity, namely, the production of sugar beet roots, in connection with the bioethanol
production.
Generally, crop irrigation and fertilization may influence the production level and quality
and, particularly, the sugar beet crop, which is water intensive and recovers efficiently the applied
fertilization. One studied the way in which the biological material adapted itself to the existing
conditions, to the irrigated agricultural system as well as to the application of different fertilization
rankings.
Furthermore, the research conducted in the combined domains of agriculture and biofuels is
coming, on the one hand, to help the development of appropriate technological measures meant to
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
11
ensure proper tracking and management of the irrigation regimen in relation to the plant needs and,
on the other hand, always to meet the needs of the continuous growing biofuels market.
4.2. SPECIFIC OBJECTIVES
The specific objectives consisted on carrying out experimental studies regarding the
following aspects:
� choosing the implementation area for experiences and its characterization from
geomorphological, hydrological and hydrogeological, pedoclimatic as well as from the
existing vegetation and fauna points of view;
� choosing the biological material for study, out of the sugar beet varieties grown in the
Transylvanian Plain;
� establishing the research methods and the applied crop technology in order to optimise some
certain elements of it, in terms of irrigation and three ranking fertilization;
� elaborating the methodology for obtaining bioethanol from sugar beet;
� determining the influence of irrigation, fertilization and variety upon the productions of
sugar beet and bioethanol;
� determining the influence of irrigation, fertilization and variety upon the main
physico-chemical and energetic characteristics of the bioethanol obtained from sugar beet;
� determining the water consumption, water use coefficient and efficiency;
� determining the economic efficiency of irrigation for the sugar beet crop, with very
favourable results in years with poor rainfall.
CHAPTER V
EXPERIMENTAL AREA DESCRIPTION
The experiences that underlied the thesis development were carried out on a field located on
the experimental extravilan field of the Viişoara village, Cluj county, on the left bank of Arieş river.
The village is situated in the South-Eastern part of Cluj county, about 2 km away from the town of
Câmpia-Turzii, near the European route E60. The experimental field is a part of the arable land
belonging to SC NORA LY AGROSERV SRL, located in Viişoara village. The studied area is
located in the extreme South-South-West of Transylvania, one of the components of the
Transylvanian Plateau.
The characterization of the thermal regime and pluviometric regimens for the experimental
field from Viişoara–Turda, during 2008–2010, was carried out using data recorded by Weather
Station Turda.
The temperature evolutions are presented in figure 5.1., while figure 5.2. presents the
rainfall regimens for the three years of experimental studies.
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
12
2008–normal 2009–warm 2010–warm
Fig. 5.1. Variation of monthly average temperature
in the experimental field of Viişoara–Turda, 2008–2010
2008–excessively wet 2009–excessively dry 2010-excessively wet
Fig. 5.2. Variation of monthly average rainfall regimen
in the experimental field of Viişoara–Turda, 2008–2010
CHAPTER VI
BIOLOGICAL MATERIAL AND RESEARCH METHODS
The utilized biological material was represented by monogerm type sugar beet seeds,
certificated and chemically treated for plant protection against diseases and pests. The sugar beet
seeds were supplied by the German companies KWS and STRUBE-DIECKMANN.
Fig. 6.1. Sugar beet seeds used within the experiences
carried out at Viişoara-Turda, 2008–2010
The sugar beet seeds were delivered in coloured clay coated form that contains bioactive
compounds and various micronutrients incentives that facilitate rapid appearance of plantules,
allowing water penetration as well (Figure 6.1).
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
13
6.2. RESEARCH METHODS AND APPLIED CROP TECHNOLOGY
6.2.1. Location and organization of experiences
The experiences were located on an experimental field belonging to SC NORA LY
AGROSERV SRL, located on the fields of Viişoara village, Cluj county. The study of the crops
obtained from three varieties of sugar beet cultivated in irrigated and non irrigated conditions, as
well as the research regarding the determination of water consumption for the sugar beet cultivated
in the conditions of the Transylvanian Plain, were carried out within the framework of a tri-factorial
trial. The studied experimental factors and their rankings are presented in table 6.2.
Table 6.2.
Synthetic presentation of the experimental factors
Factor A – Irrigation regime
Ranking of factor A a1 – non irrigated a2 – irrigated at the minimum humidity norm of 50 % of A.H.I.
Factor B – Fertilization
Ranking of factor B b1 –NPK 250 + 55 kg N/ha fertilization b2 –NPK 250 + 65 kg N/ha fertilization b3 –NPK 250 + 75 kg N/ha fertilization
Factor C – Sugar beet variety
Ranking of factor C c1 – Clementina c2 - Libero c3 - Leila
6.2.2. Crop technology applied in the experimental field from Vii şoara–Turda,
during 2008-2010
The crop technology applied in the experimental field from Viişoara–Turda covered the
following aspects: ♦ crop rotation;♦ fertilization;♦ soil amendment;♦ soil works;♦ seed and
sowing works;♦ maintenance of sugar beet crop;♦ pest and disease control;♦ weeding;
♦ harvesting.
CHAPTER VII
RESULTS OF THE RESEARCH REGARDING THE INFLUENCE OF IRRIGATION
REGIMEN, FERTILIZATION AND BIOLOGICAL MATERIAL UPON THE YIELD
OF SUGAR BEET CULTIVATED IN THE TRANSYLVANIAN PLAIN
The comparative analysis of the sugar beet yield (figure 7.10.) for the entire experimental
period, respectively, 2008–2010, emphasizes that the biggest yield was obtained in the experimental
year 2010 (3,708.30 t) while the smallest in 2009 (2,862.60 t).
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
14
Fig. 7.10. Total sugar beet
yield obtained on the
Vii şoara-Turda
experimental field,
2008–2010
3,465.60
2,862.60
3,708.30
2000,00
4000,00
Sug
ar b
eet y
ield
(t)
2008 2009 2010
Experimental year
In terms of the experimental factor irrigation, if comparing the irrigated and non irrigated
sugar beet crops (Figure 7.11), it can be shown that, in 2008, there was an experimental significant
difference (d = 10.47 t/ha) between the first and the latter above mentioned sugar beet crops.
Fig. 7.11. Average sugar
beet yield in irrigated and
non irrigated conditions,
Vii şoara-Turda
experimental field,
2008-2010
58.94
69.41
45.01
61.0163.22
74.12
35.00
65.00Ave
rage
yie
ld o
f sug
ar b
eet,
t
2008 2009 2010
Experimental year
Average sugar beet production, non irrigation treatment (t)Average sugar beet production, irrigation treatment (t)
If the comparative analysis is performed between all three experimental years for the sugar
beet yields obtained in irrigated and non irrigated conditions, in the context of all three rankings of
factors B (fertilization) and C (variety), one finds the biggest sugar beet yield (74.12 t/ha) in the
experimental year 2010 for the Libero variety (c2), cultivated in conditions of
NPK 250 + 75 kg N/ha fertilization (b3).
In the experimental year 2008, there was a significant difference (d = 10.47 t/ha) between
the irrigated and non irrigated yields. The highest average yield was obtained in the year 2010
(74.12 t/ha) in irrigation conditions, while the lowest (45.01 t/ha) was obtained in 2009, in non
irrigated conditions (Figure 7.12). If comparing the sugar beet yields obtained in irrigated
conditions, between all three experimental years, in the context of the three rankings of factors B
DL5% = 1.49 DL1% = 2.58 DL0,1% = 6.22
DL5% = 1.31 DL1% = 1.99 DL0,1% = 2.65
DL5% = 1.55 DL1% = 2.22 DL0,1% = 2.98
DL5% = 1.52 DL1% = 2.41 DL0,1% = 3.07
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
15
(fertilization) and C (variety), it is shown that the highest sugar beet yield was obtained in 2010
(82.80 t/ha), under conditions of fertilization with NPK 250 + 75 kg N/ha (b3).
Fig. 7.17. Average yield of sugar beet
obtained for the three cultivated
varieties, for three rankings of
fertilization and irrigated conditions,
Vii şoara-Turda, 2008–2010
65,350,3
68,7
69,562,4
74,1
61,155,2
65,2
68,954,8
73,6
74,565,6
78,6
65,3
61,761.0
72,1
56,879,4
79.068,9
82,8
69.0
65,973,9
40 60 80
Average yield of sugar beet (t)
NPK 250 + 55 kg N/ha,Clementina
NPK 250 + 55 kg N/ha,Libero
NPK 250 + 55 kg N/haLeila
NPK 250 + 65 kg,Clementina
NPK 250 + 65 kg N/ha,Libero
NPK 250 + 65 kg N/ha,Leila
NPK 250 + 75 kg N/ha,Clementina
NPK 250 + 75 kg N/ha,Libero
NPK 250 + 75 kg N/ha,Leila
2008 2009 2010
CHAPTER VIII
RESULTS OF THE RESEARCH REGARDING THE INFLUENCE OF IRRIGATION
REGIMEN, FERTILIZATION AND BIOLOGICAL MATERIAL UPON THE PRODUCTION
OF BIOETHANOL OBTAINED FROM SUGAR BEET CULTIVATED
IN THE TRANSYLVANIAN PLAIN
The comparative analysis of the entire the bioethanol production by entire experimental
period (figure 8.10.), respectively, 2008–2010, emphasizes that the biggest production was obtained
in the experimental year 2010 (352,073.26 l), and the smallest (273,642.39 l) in 2009.
Very significant positive differences (p<0,001) were obtained between the bioethanol
productions obtained in 2008 and 2009 (d = 54,276.46 l) and also between those ones obtained in
2009 and 2010 (d = -78,430.87 l), while between the productions obtained in 2008 and 2010, the
difference (d = -24,154.41 l) was not statistically significant (p>0.05). This demonstrates that the
Year 2008 DL5% = 1.28 DL1% = 1.79 DL0,1% = 2. 67
Year 2009 DL5% = 1.13 DL1% = 158 DL0,1% = 2.35
Year 2010 DL5% = 1.29 DL1% = 1.81 DL0,1% = 2.69
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
16
experimental years 2008 and 2010 were more favourable for the bioethanol production from sugar
beet, as compared to 2009.
327,918.85
273,642.39
352,073.26
0.00
100000.00
200000.00
300000.00
400000.00P
rodu
ctio
n of
bio
etha
nol (
l)
2008 2009 2010
Experimental year
Fig. 8.10. Total bioethanol production
obtained on Viişoara-Turda experimental field, 2008-2010
Concerning the experimental factor irrigation, if one comparatively analyzes the influence of
presence and absence of irrigation for the sugar beet crops that represented the raw material source
for the bioethanol production (figure 8.11.), it can be noticed that, in the experimental year 2008, a
distinct significant difference (d = 920.12 l, p<0.01) was recorded between the bioethanol
productions obtained from irrigated and non irrigated sugar beet crops.
Fig. 8.11. Average
production of
bioethanol in
irrigated and non
irrigated
conditions, during
experimental
period
2008–2010,
Vii şoara-Turda
5,612.49
6,532.66
4,326.56
5,808.34
6,037.97 7,001.78
4,000.00
6,000.00
8,000.00
Ave
rage
pro
duct
ion
of b
ioet
hano
l, l
2008 2009 2010Experimental year
Average bioethanol production, non irrigated conditions (L)Average bioethanol production, irrigated conditions (L)
The most favourable year for bioethanol production was 2010, and the least 2009.
Concerning the comparative analysis of the bioethanol productions obtained in conditions of
DL5% = 402.55 DL1% = 562.41 DL0,1% = 1,561.43
DL5% = 301.15 DL1% = 427.32 DL0,1% = 1,312.22
DL5% = 291.14 DL1% = 401.77 DL0,1% = 1,113.52
DL5% = 298.14 DL1% = 422.66 DL0,1% = 1,311.56
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
17
irrigated and non irrigated crop technologies in all three experimental years (figure 8.17) in the
context of all three rankings for factors B (fertilization) and C (variety), there were obtained similar
results, as trends.
Fig. 8.17. Average production of
bioethanol obtained with three
rankings and irrigation conditions,
comparatively presented on varieties,
Vii şoara-Turda, 2008–2010
6,011.934,795.22
6,343,30
6,532.83
5,969.176,994.99
5,690.52
5254,806,089.71
6,407.285,202,40
6,869,20
7,092.696,406.81
7,514.046,151.49
5,865.696,678.71
6,772.625,555.80
7,495.43
7,584.116,799.11
7,947.41
6,550.436,426.09
7,043.23
3500 5500 7500Average production (L)
NPK 250 + 55 kg N/ha,Clementina
NPK 250 + 55 kg N/ha,Libero
NPK 250 + 55 kg N/haLeila
NPK 250 + 65 kg,Clementina
NPK 250 + 65 kg N/ha,Libero
NPK 250 + 65 kg N/ha,Leila
NPK 250 + 75 kg N/ha,Clementina
NPK 250 + 75 kg N/ha,Libero
NPK 250 + 75 kg N/ha,Leila
2008 2009 2010
CHAPTER IX
RESULTS OF THE RESEARCH REGARDING THE DETERMINATION OF WATER
CONSUMPTION AND IRRIGATION EFFICIENCY OF THE SUGAR BEET
GROWN IN THE EXPERIMENTAL FIELD FROM VII ŞOARA-TURDA
9.1. RESULTS OF THE RESEARCH REGARDING THE DETERMINATION OF
WATER CONSUMPTION OF THE SUGAR BEET CROP, VIIŞOARA-TURDA, 2008
In 2009, the total water consumption of plants in the vegetative period, in irrigated
conditions, was 5,410 m3/ha. During summer, in the analysed area, the rainfall regimen was
characterized as being excessively rainy during June and July, though a major deficit was recorded
in August. Under these circumstances, in the first half of August, it was necessary to apply
waterings in amount of 500 m3/ha. Table 9.2. presents the determination of water consumption of
the sugar beet crop by using the method of water balance from soil in the experimental field from
Vii şoara-Turda, in irrigated conditions, for the first experimental year.
Year 2008 DL5% = 212.67 DL1% = 458.21 DL0,1% = 1,363.99
Year 2009 DL5% = 185.02 DL1% = 403.22 DL0,1% = 1,200.31
Year 2010 DL5% = 209.07 DL1% = 455.64 DL0,1% = 1,356.35
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
18
Table 9.2.
Water consumption determined by using the direct method of water balance from soil,
irrigated conditions, Viişoara-Turda, 2008
Interval Inputs Outputs Daily
consumption (m3/ha) From To
No. of
days
Ri (m3/ha)
P (m3/ha)
m (m3/ha)
Total (m3/ha)
Rf (m3/ha)
Total consumption
(m3/ha) 1.IV 30.IV 30 4,930 438 - 5,368 5,041 327 10.90 1.V 15.V 15 5,041 182 - 5,223 4,986 237 15.82 16.V 31.V 16 4,986 486 - 5,472 5,099 373 23.31 1.VI 15.VI 15 5,099 450 - 5,549 5,101 448 29.86 16.VI 30.VI 15 5,101 576 - 5,677 5,110 567 37.80 1.VII 15.VII 15 5,110 156 - 5,266 4,545 721 48.06 16.VII 31.VII 16 4,545 783 - 5,328 4,526 802 50.12 1.VIII 15.VIII 15 4,526 0 500 5,026 4,286 740 49.36 16.VIII 31.VIII 16 4,286 60 - 4,346 3,767 579 36.19
1.IX 25.IX 25 3,767 300 - 4,067 3,451 616 24.64 Total 178 - 3,431 500 - - 5,410 -
During the experimental year 2008, the evolution of the total water consumption of the
sugar beet crop, within the experimental field Viişoara-Turda, for both considered variants, namely,
irrigated and not irrigated, was similar (figure 9.1.) when the rainfall regimen was constant and the
applied watering norm was 500 m3/ha.
Fig. 9.1. Evolution of sugar beet
total water consumption during
the vegetation period,
Vii şoara-Turda, 2008
0
100200
300
400500
600
700800
900
1-30.0
4
1-15.0
5
16-31.
05
1-15.0
6
16-30.
06
1-15.0
7
16-31.
07
1-15.0
8
16-31.
08
1-25.0
9
Vegetation period
Tot
al w
ater
con
sum
ptio
n
(m3/
ha)
non-irrigated irrigated
The rainfalls supplied 63.42% of the total water quantity used in the process of plant
growing and development, the vegetation period of the sugar beet being characterized by an
excesivelly wet rainfall regimen.
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
19
9.2. RESULTS OF THE RESEARCH REGARDING THE DETERMINATION OF WATER
CONSUMPTION FOR THE SUGAR BEET CROP, VIIŞOARA-TURDA, 2009
In the second experimental year, 2009, in irrigated conditions, the total water consumption
recorded for the sugar beet crop was 5,151 m3/ha (tabelul 9.5.). The end of July and the beginning
of August represented the periods with the biggest water consumption (53.50 m3/ha/day
respectively, 50.26 m3/ha/day). Table 9.5.
Water consumption determined by using the direct method of water balance from soil,
irrigated conditions, Viişoara-Turda, 2009
Interval Inputs Outputs Daily
consumption (m3/ha) From To
No. of
days
Ri (m3/ha)
P (m3/ha)
m (m3/ha)
Total (m3/ha)
Rf (m3/ha)
Total consumption
(m3/ha)
1.IV 30.IV 30 5,335 63 - 5,398 5,168 230 7.66 1.V 15.V 15 5,168 7 - 5,175 4,999 176 11.73 16.V 31.V 16 4,999 229 - 5,228 4,926 302 18.87 1.VI 15.VI 15 4,926 242 - 5,168 4,757 411 27.40 16.VI 30.VI 15 4,757 608 - 5,365 4,767 598 39.86 1.VII 15.VII 15 4,767 287 - 5,054 4,411 643 42.86 16.VII 31.VII 16 4,411 107 500 5,018 4,162 856 53.50 1.VIII 15.VIII 15 4,162 222 500 4,884 4,130 754 50.26 16.VIII 31.VIII 16 4,130 64 - 4,194 3,602 592 37.00
1.IX 25.IX 25 3,602 0 - 3,602 3,013 589 23.56 Total 178 - 1,829 1,000 - - 5,151 -
During the experimental year 2009, the evolution of the total water consumption of the
sugar beet crop, within the experimental field Viişoara-Turda, for both considered variants, namely,
irrigated and not irrigated, was ascendent and similar (figure 9.2.) until the beginning of July when,
due to the rainfall regimen, two waterings of 500 m3/ha were needed.
Fig. 9.2. Evolution of sugar beet
total water consumption during
the vegetation period,
Vii şoara-Turda, 2009
0
100200
300
400500
600
700800
900
1-30.0
4
1-15.0
5
16-3
1.05
1-15.0
6
16-3
0.06
1-15.0
7
16-3
1.07
1-15.0
8
16-3
1.08
1-25.0
9
Vegetation period
Tot
al w
ater
con
sum
ptio
n
(m3/
ha)
non-irrigated irrigated
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
20
9.3. RESULTS OF THE RESEARCH REGARDING THE DETERMINATION OF WATER
CONSUMPTION FOR THE SUGAR BEET CROP, VIIŞOARA-TURDA, 2010
In the experimental year 2010, in irrigated conditions, the total water consumption recorded
for the sugar beet crop was 5,861 m3/ha (tabelul 9.8.). As in the previous two years, the periods with
the biggest water consumption were the end of July and the beginning of August (51.38 m3/ha/day,
respectively, 50.20 m3/ha/day). Table 9.8.
Water consumption determined by using the direct method of water balance from soil,
irrigated conditions, Viişoara-Turda, 2010
Interval
Inputs Outputs Daily
consumption (m3/ha) From To
No. of
days
Ri (m3/ha)
P (m3/ha)
m (m3/ha)
Total (m3/ha)
Rf (m3/ha)
Total consumption
(m3/ha) 1.IV 30.IV 30 4,905 377 - 5,282 4,949 333 11.10 1.V 15.V 15 4,949 311 - 5,260 5,009 251 16.73 16.V 31.V 16 5,009 329 - 5,338 4,968 370 23.12 1.VI 15.VI 15 4,968 350 - 5,318 4,881 437 29.13 16.VI 30.VI 15 4,881 910 - 5,791 5,219 572 38.13 1.VII 15.VII 15 5,219 226 - 5,445 4,714 731 48.73 16.VII 31.VII 16 4,714 657 - 5,371 4,549 822 51.38 1.VIII 15.VIII 15 4,549 280 500 5,329 4,576 753 50.20 16.VIII 31.VIII 16 4,576 58 - 4,634 3,945 689 43.06
1.IX 25.IX 25 3,945 252 - 4,197 3,434 763 30.52 Total 178 - 3,750 500 - - 5,861 -
Concerning the experimental year 2010, the evolution of the total water consumption for the
sugar beet crop, within the experimental field from Vii şoara-Turda, for both considered variants,
namely, irrigated and not irrigated, was similar (figure 9.3.) when the rainfall regimen was constant
and the applied watering norm was 500 m3/ha.
Fig. 9.3. Evolution of sugar beet
total water consumption during
the vegetation period,
Vii şoara-Turda, 2010
0
100200
300
400500
600
700800
900
1-30.0
4
1-15.0
5
16-3
1.05
1-15.0
6
16-3
0.06
1-15.0
7
16-3
1.07
1-15.0
8
16-3
1.08
1-25.0
9
Vegetation period
Tot
al w
ater
con
sum
ptio
n
(m
3/ha
)
non-irigated irrigated
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
21
9.4. DETERMINATION OF WATER USE COEFFICIENT
IN IRRIGATED CONDITIONS, VIIŞOARA-TURDA, 2008-2010
When irrigation was practiced, during the sugar beet vegetation period, the total water
consumption of plants was 5,861 m3/ha. The highest water consumption was registered in July
(51.38 m3/ha). The daily watering values were of 11.10 m3/ha/day, in the first part of the vegetation
period and, respectively, 51.38 m3/ha/day, in the second half of July (table 9.8.).
Table 9.10.
Water valorification coefficient of the sugar beet crop, Viişoara-Turda, 2008–2010
Year Irrigation regimen
Variety Total water
consumption (m3/ha)
Average yield (kg/ha)
Water valorification coefficient
(m3/kg)
2008
Non - irrigated Clementina
4,910 58,100 0.084
Libero 63,067 0.078 Leila 55,667 0.088
Irrigated Clementina
5,410 68,767 0.078
Libero 74,333 0.073 Leila 65,133 0.083
2009
Non - irrigated Clementina
4,151 38,867 0.107
Libero 47,433 0.087 Leila 48,733 0.085
Irrigated Clementina
5,151 55,167 0.093
Libero 66,400 0.077 Leila 61,467 0.084
2010
Non - irrigated Clementina
5,361 62,767 0.085
Libero 66,667 0.080 Leila 60,233 0.089
Irrigated Clementina
5,861 73,900 0.079
Libero 78,500 0.075 Leila 69,967 0.084
9.5. DETERMINATION OF WATER USE EFFICIENCY FOR THE SUGAR BEET CROP IN
IRRIGATED CONDITIONS, VIIŞOARA-TURDA, 2008-2010
Concerning the Leila sugar beet variety, it was found that, in non irrigated conditions, the
water valorification coefficient was ranged in the interval 0.085–0,089, while in irrigated conditions
the values of the water valorification coefficient was situated in the interval 0.083–0.084.
Once more, the irrigated crops are found to recover water better than the non irrigated ones,
with the best situation in the experimental year 2008.
If making a comparison between the three studied sugar beet varieties, during the considered
period 2008-2010, it is found that Libero variety had a better water valuation, in irrigated
conditions.
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
22
Table 9.11.
Valorification efficiency of irrigation water by the studied varieties (c1, c2, c3),
Vii şoara-Turda, 2008-2010
Year Variety Yield increase due
to irrigation (kg/ha)
Irrigation norm (m3/ha)
Valorification efficiency of irrigation
water (kg/m3)
2008 Clementina 10,667
500 21.33
Libero 11,266 22.53 Leila 9,466 18.93
2009 Clementina 16,300
1.000 16.30
Libero 18,967 18.97 Leila 12,734 12.73
2010 Clementina 11,133
500 22.27
Libero 11,833 23.67 Leila 9,734 19.47
CHAPTER X
RESULTS OF THE RESEARCH REGARDING THE ECONOMIC EFFI CIENCY
OF THE IRRIGATED SUGAR BEET CROP IN THE CONDITIONS
OF THE TRANSYLVANIAN PLAIN
Table 10.10. presents the average profit and the supplementary production obtained for the
sugar beet crop obtained in irrigated conditions during 2008-2010, in the experimental field from
Vii şoara-Turda. The best efficiency concerning the irrigation water valorization was recorded by
Libero variety, followed by Clementina and Leila varieties. In 2010, all the varieties of sugar beet
realized the biggest valorization efficiency of the irrigation water.
For Clementina variety, by irrigating the crop fertilized with NPK 250 + 55 kg N/ha
(ranking b1), it was obtained a profit in amount of 1,416.00 RON/ha; by irrigating the crop fertilized
with NPK 250 + 65 kg N/ha (ranking b2), a profit in amount of 1,524.00 RON/ha was obtained;
the crop irrigated and fertilized with NPK 250 + 75 kg N/ha (ranking b3) led to a profit in amount of
1,632.00 RON/ha.
For Libero variety, by irrigating the crop fertilized with NPK 250 + 55 kg N/ha (ranking b1),
it was obtained a profit in amount of 1,564.80 RON/ha; by irrigating the crop fertilized with
NPK 250 + 65 kg N/ha (ranking b2), a profit in amount of 1,687.20 RON/ha was obtained; the crop
irrigated and fertilized with NPK 250 + 75 kg N/ha (ranking b3) led to a profit in amount of
1,795.20 RON/ha.
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
23
Table 10.10.
Average profit obtained for sugar beet, Viişoara-Turda, period 2008-2010
Variant Average
yield (t/ha)
Relative yield (%)
Difference (t/ha)
Average profit (RON/ha)
a1 x b1 x c1 50.07 100.00 0.00 Mt. a2 x b1 x c1 61.87 123.56 11.80 1,416.00
a1 x b2 x c1 53.30 100.00 0.00 Mt. a2 x b2 x c1 66.00 123.83 12.70 1,524.00
a1 x b3 x c1 56.37 100.00 0.00 Mt. a2 x b3 x c1 69.97 124.13 13.60 1,632.00
a1 x b1 x c2 55.73 100.00 0.00 Mt. a2 x b1 x c2 68.77 123.40 13.04 1,564.80
a1 x b2 x c2 59.17 100.00 0.00 Mt. a2 x b2 x c2 73.23 123.76 14.06 1,687.20
a1 x b3 x c2 62.27 100.00 0.00 Mt. a2 x b3 x c2 77.23 124.02 14.96 1,795.20
a1 x b1 x c3 51.27 100.00 0.00 Mt. a2 x b1 x c3 60.73 118.45 9.46 1,135.20
a1 x b2 x c3 55.23 100.00 0.00 Mt. a2 x b2 x c3 65.93 119.37 10.70 1,284.00
a1 x b3 x c3 58.13 100.00 0.00 Mt. a2 x b3 x c3 69.90 120.25 11.77 1,412.40
For Leila variety, by irrigating the crop fertilized with NPK 250 + 55 kg N/ha (ranking b1),
it was obtained a profit in amount of 1,135.20 RON/ha; by irrigating the crop fertilized with
NPK 250 + 65 kg N/ha (ranking b2), a profit in amount of 1,284.20 RON/ha was obtained; the crop
irrigated and fertilized with NPK 250 + 75 kg N/ha (ranking b3) led to a profit in amount of
1,412.40 RON/ha.
The evolution of the average benefit for each of three studied varieties, as well as the annual
average profit, obtained in irrigated conditions, during 2008-2010, are presented in figure 10.4.
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
24
Fig. 10.4. Evolution of the
annual average profit for
sugar beet, irrigated conditions,
Vii şoara-Turda, 2008-2010
700,001.000,001.300,001.600,001.900,002.200,002.500,00
Av erage benefit(RON/ha)
2008
2009
2010
Year
Clementina Libero Leila Sugar beet culture
CHAPTER XI
RESULTS OF THE RESEARCH REGARDING THE QUALITY OF TH E BIOETHANOL
OBTAINED FROM SUGAR BEET GROWN AT VII ŞOARA-TURDA
For the trait ”content of ethanol and higher saturated alcohols”, Clementina, Libero and
Leila varieties recorded values between 99.2-99.4%, beyond the minimum value allowed by
SR 15376, namely, 98.7% (m/m), the average of the irrigated variants recording substantially bigger
values than those belonging to the non irrigated variants. The corresponding values for the contents
of the higher saturated alcohols (C3-C5), determined for all variants of bioethanol obtained from
sugar beet, were much lower compared to the value of the maximum stipulated by SR EN 15376, of
2.0% (m/m). The values corresponding for the methanol content for all analysed samples were
lower than the authorized maximum limit value of 1.0% (m/m). The repeated fractionate
distillation, followed by dry distillation performed in zeolites column, carried out in laboratory
conditions, led for all three analyzed varieties, to values of the water content in the interval
0.237–0.253%, all below the admitted maximum value stipulated by SR EN 15376, namely,
0.300% (m/m).
The copper content from all bioethanol samples resulted from the comparison made between
the integrated absorbance Ai, produced by the copper contained in the analyzed samples and the
calibration curve established with aqueous solutions of copper with known titre. The determination
of sulphur content was conducted by ultraviolet fluorescence for all samples and the values obtained
for the sulphur concentration were below the detection limit (0.80 mg/kg) of the UV fluorescence
equipment. The values obtained for the higher calorific value, for all bioetanol samples, were
sensitively below the recognized and accepted average value, namely, 29,700 J/g. The average
value determined for the higher calorific value, corresponding for the bioethanol resulted from all
three analyzed sugar beet varieties was 29,008 J/g and the average values of the irrigated variants
were substantially higher as compared to the average values of the non irrigated variants.
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
25
CHAPTER XII
GENERAL CONCLUSIONS
12.1. CONCLUSIONS REGARDING THE INFLUENCE OF IRRIGATION REGIMEN,
FERTILIZATION AND BIOLOGICAL MATERIAL UPON THE PRODUCTION OF ROOTS
AND BIOETHANOL OBTAINED FROM SUGAR BEET GROWN
IN THE TRANSYLVANIAN PLAIN
In 2008, concerning the influence of the irrigation regimen on the productions of sugar beet
roots and bioethanol, it was found that, for all three practiced repetitions, whatever fertilization or
variety ranking, there were obtained superior values for irrigated conditions. Thus, there were
recorded positive and very significant differences (p<0.001) between the sugar beet yields obtained
in irrigated conditions, as compared to the non irrigated ones (d = 10.47 t/ha, in relative values
17.76 %), considered control, irrespective of fertilization or variety ranking. The experimental
factor irrigation represented the main cause of variability. The variance was s2 = 1,313.00.
Concerning the bioethanol production, there were recorded positive and very significant differences
(p<0.001) betweeen the yields obtained from irrigated sugar beet crops (d = 1,099.06 l) and the non
irrigated ones, considered control, superior by 19.0% in relative terms, whatever fertilization or
variety ranking. The variance was s2 = 15,530,844.00.
In the experimental year 2009, concerning the influence of the irrigation regimen on the
productions of sugar beet roots and bioethanol, it was found that, for all three repetitions,
irrespective of fertilization or variety ranking, there were obtained superior productions in irrigated
conditions. Thus, there were recorded positive and very significant differences (p<0.001) between
the sugar beet yields obtained in irrigated conditions as compared to the non irrigated ones
(d = 15.36 t/ha, in relative values 34.27%), considered control, whatever fertilization or variety
ranking. For the experimental factor irrigation, it was recorded a variance of s2 = 1,155.82.
Concerning the bioethanol production, there were recorded positive and very significant differences
(p<0.001) betweeen the productions obtained from irrigated sugar beet crops (d = 1,481.78 l) and
the non irrigated ones, considered control, superior by 34.24% in relative terms, whatever
fertilization or variety ranking. The variance was s2 = 12,868,413.60.
The Libero variety recorded the best production performances in all it concerns the sugar
beet yields, with an average of 68.90 t/ha (NPK 250 + 75 kg N/ha fertilization), as well as
bioethanol, with an average production of 6,799.11 l. The lowest sugar beet productions were
obtained for Clementina variety, with an average of 37.30 t/ha (NPK 250 + 55 kg N/ha fertilization)
and, respectively, of 4,795.22 l (NPK 250 + 55 kg N/ha fertilization), for the bioethanol production,
in non irrigated conditions. The variances corresponding for the sugar beet and bioethanol
productions were s2 = 1,521.58 and, respectively, s2 = 17,809,074.00.
In 2010, concerning the influence of the irrigation system influence upon the sugar beet
roots and bioethanol productions, it was found that, for all three repetitions, there were obtained
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
26
superior productions in irrigated conditions, whatever fertilization or variety ranking. Thus, there
were recorded positive and very significant differences (p<0.001) between the sugar beet yields
obtained in irrigated conditions, as compared to non irrigated ones (d = 10.92 t/ha, in relative values
17.76%), considered control, whatever fertilization or variety ranking. The experimental factor
irrigation represented the main cause of variability. The variance was s2 = 1,323.41. Concerning the
bioethanol production, there were recorded positive and very significant differences (p<0.001)
betweeen the productions obtained from irrigated sugar beet crops (d = 963.81 l) and the non
irrigated ones, considered control, superior by 15.96% in relative terms, whatever fertilization or
variety ranking. The corresponding variance was of s2 = 16,714,146.40.
Libero variety recorded the best production performances regarding both sugar beet and
bioethanol productions, with corresponding average values of 82.80 t/ha (NPK 250 + 75 kg N/ha
fertilization) and 7,987.41 l, respectively. The lowest sugar beet and bioethanol productions were
obtained for Clementina variety in non irrigated conditions, with average values of 56.30 t/ha
(NPK 250 + 55 kg N/ha fertilization) and 5,318.49 l (NPK 250 + 55 kg N/ha fertilization),
respectively. The variances corresponding for the sugar beet and bioethanol productions were
s2 = 1,742.21 and s2 = 23,131,326.60, respectively.
Considering the experimental factor irrigation, it was found that the biggest sugar beet
production was obtained in 2010 (74.12 t/ha) in irrigated conditions, while the smallest (45.01 t/ha)
in 2009, in non irrigated conditions. The biggest average production of bioethanol was obtained in
2010 (7,001.78 l) in irrigated conditions, while the smallest (4.326.56 l) in 2009, in non irrigated
conditions. The results emphasize the importance of irrigation in years unfavourable for the sugar
beet crops destined to bioethanol productions, as year 2009 was. If one analyzes the experimental
factor fertilization, with its three rankings, namely, b1 - NPK 250 + 55 kg N/ha,
b2 - NPK 250 + 65 kg N/ha and, namely, b3 - NPK 250 + 75 kg N/ha, it is found that the biggest
sugar beet productions were obtained in 2010, when using the dose of NPK 250 + 75 kg N/ha
(78.71 t/ha) in irrigated conditions, while the most unsatisfactory ones (45.23 t /ha) were obtained in
2009, in non irrigated consitions and when the fertilization dose was NPK 250 + 55 kg N/ha.
If it is performed a comparative analysis of variance as function of experimental year and
variability cause, it is found that, in 2008, the most important factor was irrigation (s2 = 1,313.00),
followed by variety (s2 = 864.53) and fertilization (s2 = 125.86), while in the following two
experimental years, the variability causes had different influences upon the obtained sugar beet
yields. Thus, in 2009, the biggest variance was recorded for variety (s2 = 1,521.58), being followed
by irrigation (s2 = 1,155.82) and fertilization (s2 = 221.51). In the experimental year 2010, the most
important factor that influenced variability was variety (s2 = 1,742.21), followed by irrigation
(s2 = 1,323.41) and fertilization (s2 = 253.63).
Considering the entire analyzed experimental period, for both sugar beet and bioethanol
productions, it was found that variety represented the most important factor that influenced
variability in 2009 and 2010, with a maximum share in 2010, while in 2008 the most important
factor that influenced variability was irrigation. Fertilization had the lowest influence on sugar beet
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
27
production, its minimum value being recorded in 2008. In 2010, the variance caused by fertilization
had the biggest value, though much smaller as compared to those caused by irrigation and variety.
12.2. CONCLUSIONS REGARDING THE ECONOMIC EFFICIENCY OF IRRIGATION
ON THE YIELD OF SUGAR BEET GROWN IN THE CONDITIONS
OF VIIŞOARA-TURDA, DURING 2008-2010
Analyzing the values obtained for the irrigated sugar beet crops, it was found that one
obtained profits during 2008-2010, for each of the three analyzed varieties.
Thus, as regarding Clementina variety, the irrigated crop fertilized with NPK 250 + 55 kg
N/ha (ranking b1) led to an average profit of 1,335.27 RON/ha; the irrigation of the crop fertilized
with NPK 250 + 65 kg N/ha (ranking b2) led to an average profit of 1,444.93 RON/ha; for the
irrigated crop, fertilized with NPK 250 + 75 kg N/ha (ranking b3) resulted an average profit of
1,554.93 RON/ha. If the variant irigated x fertilized with NPK 250 + 55 kg N/ha (a2 x b1) is
considered control, for the variant irigated x fertilized with NPK 250 + 65 kg N/ha (a2 x b2) it was
obtained an average profit of 109.66 RON/ha (108.21%), while for the variant irigated x fertilized
with NPK 250 + 75 kg N/ha (a2 x b3) it was obtained an average benefit of 219.66 RON/ha
(116.45%).
For Libero variety, the irrigation of the crop fertilized with NPK 250 + 55 kg N/ha
(ranking b1) led to an average profit of 1,490.93 RON/ha; for the irrigated crop, fertilized with
NPK 250 + 65 kg N/ha (ranking b2) resulted an average profit of 1,615.93 RON/ha; the irrigation of
the crop, fertilized with NPK 250 + 75 kg N/ha (rank b3) led to an average profit of
1,724.93 RON/ha. If the variant irigated x fertilized with NPK 250 + 55 kg N/ha (a2 x b1) is
considered control, for the variant irigated x fertilized with NPK 250 + 65 kg N/ha (a2 x b2) it
resulted a profit of 125.00 RON/ha (108.38%), while for the variant irigated x fertilized with
NPK 250 + 75 kg N/ha (a2 x b3), resulted a profit of 234.00 RON/ha (115.69%).
For Leila variety, the irrigation of the crop fertilized with NPK 250 + 55 kg N/ha (ranking
b1) led to an average profit of 1,045.93 RON/ha; for the crop irrigated and fertilized with
NPK 250 + 65 kg N/ha (ranking b2) resulted an average profit of 1,197.27 RON/ha; the irrigation of
the crop fertilized with NPK 250 + 75 kg N/ha (ranking b3) led to an average profit of
1,328.27 RON/ha. If the variant irigated x fertilized with NPK 250 + 55 kg N/ha (a2 x b1) is
considered control, for the variant irigated x fertilized with NPK 250 + 65 kg N/ha (a2 x b2) resulted
a profit of 151.34 RON/ ha (114.47%), while for the variant irigated x fertilized with
NPK 250 + 75 kg N/ha (a2 x b3), resulted a profit of 282.34 RON/ha (126.99%).
If compared with 2008 and 2010, the year 2009 recorded a very significant increase of the
profit obtained as consequence of irrigating the sugar beet crop (p<0.001). This can be explained,
firstly, by the increased differences between the sugar beet yields obtained in irrigated conditions as
compared with the ones obtained in non irrigated conditions in 2009, a warm and excessively dry
year from metheorological point of view.
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
28
The variation of the purchase price for the sugar beet roots was another factor that
contributed to the profit evolution during 2008-2010.
Analyzing the values of the average profit, calculated for each studied variety, one finds
that, in irrigated conditions, during 2008-2010, Libero variety recorded the biggest average profit
calculated for all three rankings of fertilization, followed by Clementina and Leila varieties. The
cultivation of all three sugar beet varieties, in all irigated x fertilized variants, proved to be
economically efficient, in the conditions of Viişoara–Turda experimental area, during 2008–2010.
12.3. CONCLUSIONS REGARDING THE QUALITY OF BIOETHANOL OBTAINED
FROM SUGAR BEET GROWN IN THE CONDITIONS OF VIIŞOARA-TURDA
The values determined for the ethanol and higher saturated alcohols content were bigger as
compared to the minimum value stipulated by standard SR EN 15376, being obtained by applying
repeated fractionate distillation, followed by dry zeolites column. For all three analyzed varieties,
the average values of the irrigated variants recorded higher values as compared to those ones
corresponding for the non irrigated variants.
All the values determined for the methanol content were between 0.046–0.049% (m/m), the
irrigated variants registering smaller values as compared to those corresponding to the non irrigated
variants for the same ranking of the fertilization factor, this fact strongly recommending them for
sugar beet crops destined for obtaining bioethanol.
For all three analyzed varieties, the average value determined for water content was of
0.245% (m/m), the irrigated variants recording, in avearge, higher values as compared to the non
irrigated variants.
All values determined for the inorganic chloride were below the maximum value stipulated
by SR EN 15376, of 20 mg/l, the irrigated variants having smaller values as compared to the non
irrigated variants, for the same ranking of the fertilization factor, this fact recommending them for
sugar beet crops destined for obtaining bioethanol.
There were not recorded any big variations concerning the values of the higher calorific
value for all tested variants, all obtained values being in the range 28.873-29.142 J/g (0.93%), for
all three tested varieties, the irrigated variants recording in average, higher values as compared to
those ones corresponding to the non irrigated variants. The best results were recorded for Libero
and Leila varieties.
The values obtained for the Research octane number (RON) and, respectively, Motor octane
number (MON) framed in the indicated intervals, the average value of the irrigated variants
recording higher values as compared to those ones corresponding to the non irrigated variants. The
best results were recorded for Libero and Leila varieties.
Based on the obtained results, one can state that, taking into consideration the studied
biological material, the best solution for sugar beet crops destined for obtaining biethanol is
represented by the irrigated x fertilized variants.
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
29
SELECTIVE BIBLIOGRAPHY
1. ARDELEAN M., 2005, Principii ale metodologiei cercetării agronomice şi medical
veterinare, Editura AcademicPres, Cluj-Napoca;
2. BÂRSAN SIMONA-CLARA , ANCUŢA-MARIA PUŞCAŞ, E. LUCA,
A. SETEL, 2008, Bioetanolul şi cultura sfeclei de zahăr, Agricultura–Revistă de ştiinţă şi
practică agricolă, nr. 3-4 (67-68)/2008, pag. 11-15;
3. BÂRSAN SIMONA-CLARA , ANCUŢA-MARIA PUŞCAŞ, E. LUCA,
A. SETEL, 2009, Caracterizarea condiţiilor pedologice din câmpul experimental Viişoara,
utilizat pentru obţinerea de bioetanol din sfecla de zahăr, Agricultura–Revistă de ştiinţă şi
practică agricolă, nr. 3-4 (71-72)/2009, pag. 16-20;
4. BÂRSAN SIMONA-CLARA , E. LUCA, MIHAELA-GEORGIA SIMA,
ANCUŢA-MARIA PUŞCAŞ, ADRIANA-PAULA DAVID, ADRIANA MARIA GOG,
2010, Sugar beet fermentation process for obtaining bioethanol – results obtained in the
experiences carried out on the sugar beet harvested from the Viişoara village experimental
field, in the agricultural year 2007-2008, Revista Agricultura-Revista de ştiinţă şi practică
agricolă, Anul XIX, nr. 3-4 (75-76)/2010, pag. 80-85;
5. BÂRSAN SIMONA-CLARA , E. LUCA, ANCUŢA-MARIA PUŞCAŞ, GEORGIA SIMA,
2011, Quality requirements of Bioethanol samples Obtained from the Sugar Beet Cultivated
in the experimental fields of Viişoara-Turda, within the Agricultural Year 2007-2008,
Buletin USAMV, Horticultura 68(2)/2011, pag. 283-289;
6. BÂRSAN SIMONA-CLARA , E. LUCA, ANCUŢA PUŞCAŞ, GEORGIA SIMA, 2011,
Higher Calorific Power of Bioethanol Samples Obtained from the Sugar Beet Cultivated in
the Experimental Fields of Viişoara-Turda, within the Agricultural Year 2007-2008, Buletin
USAMV, Horticultura 68(2)/2011, pag. 464;
7. BÂRSAN SIMONA-CLARA , ANCUŢA-MARIA PUŞCAŞ, E. LUCA,
MIHAELA-GEORGIA SIMA, 2011, Energy Potential of the Agricultural Sugary and
Starchy Crops in the Specific Conditions of Transylvanian Plain, Revista Agricultura-
Revista de ştiinţă şi practică agricolă, Anul XX, nr. 3-4 (79-80)/2011, pag. 174-179;
8. BÂRSAN SIMONA-CLARA , ANCUŢA-MARIA PUŞCAŞ, E. LUCA,
MIHAELA-GEORGIA SIMA, 2012, Research regarding the quality of bioethanol obtained
from the sugarbeet cultivated in an experimental field from Transylvania region, 47th
Croatian & 5th International Symposium on Agriculture, Opatija, Croatia, pag. 459-463;
9. BÂRSAN SIMONA-CLARA , E. LUCA, 2012, Research Concerning the Economic
Efficiency of Irrigation for the Sugar Beet Cultivated in the Transylvanian Plain, Revista
Agricultura - Revista de ştiinţă şi practică agricolă, Anul XXI, nr. 1-2 (81-82)/2012, under
print;
10. BORLAN Z. şi colab., 1994, Fertilitatea şi fertilizarea solurilor-Compendiu de Agrochimie,
Editura Ceres, Bucureşti;
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
30
11. BUDIU V., 1994, Stabilirea coeficienţilor de corecţie a evapotranspiraţiei potenţiale în
vederea avertizării udărilor în sistemele de irigaţie, Buletin USAMV Cluj-Napoca,
A-H, 48/1, pag. 97-105;
12. CIONI F. and G. MAINES, 2010, Weed Control in Sugarbeet, SugarTech, vol. 12, no. 3-4,
Special issue on Sugar Beet in 21th Century–SpringerLink;
13. CÎMPEANU S., I. PLEŞA, Al. ENE, 2002, Proiectarea lucrărilor de irigaţie, desecări–
drenaje şi combaterea eroziunii solului, Editura RELAL;
14. CÎMPEANU S., D. BUCUR, 2005, Combaterea eroziunii solului, Editura RELAL;
15. CRIVEANU H., E. LUCA, M. HĂRŞAN, 2001, Observaţii privind temperatura aerului,
precipitaţiile şi consumul de apă în Transilvania, Revista Bioterra, nr. 3, anul III,
Cluj-Napoca;
16. DAVID ADRIANA–PAULA, 2010, Utilajul şi tehnologia de obţinere a zahărului, Editura
RISOPRINT, Cluj–Napoca;
17. DÎRJA M., 2004, Îmbunătăţiri funciare, Ed. Academic Pres, Cluj-Napoca;
18. GHINEA D., 2000, Enciclopedia geografică a României, Editura Enciclopedică, Bucureşti;
19. GOG ADRIANA, LĂCRIMIOARA ŞENILĂ, SIMONA BÂRSAN , ANCUŢA PUŞCAŞ,
E. LUCA, 2011, Ethanol Determination in Sugar Beet Fermentation Liquid by Full
Evaporation Headspace Gas Chromatographic Method, Studia Universitatis Babeş-Bolyai
Chemia, Volume 56 (LVI), pag. 235 – 242 (ISI);
20. GRUMEZA N., C. TUŞA, 2000, Consumul de apă şi evoluţia teritoriului amenajat pentru
irigaţii din România, Buletinul AGIR, nr. 3, Bucureşti;
21. GUŞ P., T. RUSU, ILEANA BOGDAN, 2004, Agrotehnică, Editura Risoprint,
Cluj - Napoca;
22. HAILONG LI, XIN-SHENG CHAIA, YULIN DENG, HUAIYU ZHA N, SHIYU FU, 2009,
Rapid determination of ethanol in fermentation liquor by full evaporation headspace gas
chromatography, Journal of Chromatography A, 1216, pag. 169–172;
23. IONESCU–SISEŞTI V. şi colab., 1982, Irigarea culturilor, Editura Ceres, Bucureşti;
24. JINGA I., 1971, Cercetări privind valorificarea prin infiltraţie a apelor reziduale provenite
de la complexele pentru creşterea şi îngrăşarea industrială a porcilor, Teză de doctorat,
IAMB, Bucureşti;
25. JINGA I., I. PLEŞA, S. CÎMPEANU, 1993, Baze pentru experimentarea amenajării
terenurilor agricole în vederea fertilizării cu ape uzate şi nămoluri provenite din complexele
animaliere în vederea combaterii poluării solului, Contract ISPIF SA Bucureşti;
26. LAMARCK J. B. et J. L. M. POIRET, 1783, Encyclopédie méthodique: Botanique, Vol. 1,
(par M. Lamarck, continuée par J. L. M. Poiret), Publication info: Henri Agasse-Science,
Paris;
27. LUCA E., 1994, Cercetări privind tehnologia şi regimul de irigare la porumbul cultivat în
condiţii ecologice din zona subumedă a Transilvaniei, Teză de doctorat, Biblioteca USAMV
Cluj-Napoca;
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
31
28. LUCA E. şi Z. Nagy, 1999, Irigarea culturilor, Editura Genesis, Cluj-Napoca;
29. LUCA E. şi colab, 2004, Tehnologii ecologice pentru cultura plantelor, Editura Risoprint,
Cluj-Napoca;
30. LUCA E., V. BUDIU, ANA CIOTLĂUŞ, 2008, Exploatarea sistemelor de îmbunătăţiri
funciare - Irigaţii, Editura Risoprint, Cluj-Napoca;
31. MANEA Gh., 2003, Elemente pentru o politică naţională de promovare a
biocombustibililor, Promovarea în România a surselor regenerabile de energie, Editura
CHIMINFORM DATA, Bucureşti;
32. MANOLE E., S. CÎMPEANU, 2006, Calculul amenajărilor de irigaţii, Editura NOUA;
33. MOGÂRZAN AGLAIA, G. MORAR, M. ŞTEFAN, 2004, Fitotehnie, Editura „Ion Ionescu
de la Brad”, Iaşi;
34. NAGY Z., 1989, Recomandări privind cultura sfeclei de zahăr în jud. Cluj, Tipo
Agronomia, Cluj-Napoca;
35. NAGY Z. şi E. LUCA, 1994, Irigarea culturilor, Tipo Agronomia, Cluj-Napoca;
36. NAGY Z., E. LUCA, Al. TURDEAN, 1994, Cercetări privind consumul de apă al
principalelor culturi de câmp din zona colinară a Transilvaniei, Buletinul IACN, seria
Agricultură, nr. 48;
37. NICOLESCU C., 2005, Considerations concerning the rational use of irrigation water, Lucrări
ştiinţifice, seria Agronomie, vol. 48, Simpozion cu participare internaţională, Agricultura şi mediul –
prezent şi perspective”, editat cu sprijinul Ministerului Educaţiei şi Cercetării, Editura Ion Ionescu de
la Brad, Iaşi, pag.172–179;
38. ONCIA SILVICA, 1999, Cercetări cu privire la consumul de apă al principalelor culturi
irigate în condiţiile Câmpiei Banatului, Teză de doctorat, USAMV a Banatului, Timişoara;
39. ONCIA SILVICA, 2004, Îmbunătăţiri funciare, Ed. Orizonturi Universitare, Timişoara;
40. PASCU A. şi A.F. BADIU, 1996, Dicţionar al culturii sfeclei de zahăr, Editura Fermierul
Român, Bucureşti;
41. PASTOR I., 2004, Strategii noi în combaterea integrată a buruienilor din cultura sfeclei de
zahăr din Transilvania, Editura Tipomur, Târgu-Mureş;
42. PAULETTE LAURA şi Gh. BLAGA, 2002, Pedologie, Lucrări practice, Editura Poliam,
Cluj-Napoca;
43. PLEŞA I., I. JINGA, Al. ENE, S. CÎMPEANU, 2000, Îmbunătăţiri funciare şi irigarea
culturilor. Îndrumător de lucrări practice, AMC, USAMV Bucureşti;
44. PLEŞA I., S. CÎMPEANU, Îmbunătăţiri funciare, 2001, Editura Cris Book, Bucureşti;
45. POP G.P., Depresiunea Transilvaniei, 2001, Presa Universitară Clujeană, Cluj-Napoca;
46. POPESCU V. şi colab., 1988, Tehnologii moderne pentru creşterea producţiei şi a valorii de
industrializare a sfeclei de zahăr, Editura Ceres, Bucureşti;
47. PUŞCAŞ ANCUŢA-MARIA, E. LUCA, SIMONA-CLARA BÂRSAN , V. CEUCĂ,
Al. SIMU, 2008, Experimental results obtained in sugar beet crop technology and water
Eng. Simona-Clara BÂRSAN Summary of the PhD Thesis
32
consumption specific to Transylvania’s field conditions, Buletin USAMV, Horticultura
65(2), pag. 528-532;
48. PUŞCAŞ ANCUŢA-MARIA, SIMONA-CLARA BÂRSAN , A. SETEL, MIHAELA -
GEORGIA SIMA, 2010, The influence of irrigation on sugar beet yield obtained in the
conditions of NW Transylvania, 45th Croatian & 5th International Symposium on
Agriculture, Opatija, Croatia, pag. 892-895;
49. RACOSY L., M. GOIA, Z. KOVACS, 2003, Verzeichnis der Schmetterlinge Rumäniens,
Catalogul lepidopterelor din România (ediţie bilingvă). Editura Soc. Lepid. Română,
Cluj-Napoca, pag. 446;
50. RANCOVIC JOVANA, Jelena Dodic, Sinisa Dodic, S. Popov, 2009, Bioethanol production
from intermediate products of sugar beet processing with different types of Saccharomyces
cerevisiae, Chemical Industry & Chemical Engineering Quarterly, 15 (1), 13-16;
51. RUSSELL I., 1997, The nutritional requirements of yeast, Brewers Guardian, November
1997, pp 25-31;
52. SALONTAI Al., L.S. MUNTEAN, S. CERNEA, G. MORAR, 1985, Lucrări practice de
fitotehnie, Tipo Agronomia, Cluj-Napoca;
53. SOROCOVSCHI V., 2005, Câmpia Transilvaniei, Editura Casa Cărţii de Ştiinţă,
Cluj-Napoca;
54. STĂNESCU Z., Gh. RIZESCU, 1976, Sfecla de zahăr, Editura Ceres, Bucureşti;
55. TRIPŞA I., 2006, Utilizarea surselor regenerabile de energie. Promovarea în România a
surselor regenerabile de energie, Editura CHIMINFORM DATA, Bucureşti.