Steroid Hormone Profilingby Mass Spectrometry (LC-MS)

For research use only. Not for use in diagnostic procedures.

Ready-to-Use

Comprehensive Coverage

Best Fit to Physiological Range

SuperiorData Quality

KIT

AbsoluteIDQ® Stero17 Kit

Superior Confi dence in Steroid Hormone Analysis

Steroid hormones are a group of metabolites that are derived from cholesterol. They are produced primarily by adrenal glands, testes, and ovaries. Based on their receptor, steroid hormones are classifi ed into fi ve groups: glucocorticoids, mineralocorticoids, progestogens, androgens, and estrogens. Steroid hormones exert multiple biological functions, including regulation of glucose levels, cell growth, and proliferation. The trend in steroid analysis is moving from measuring single or small sets of steroid hormones with immunoassays towards broader, mass spectrometry-based quantifi cation of steroid hormones (Jeanneret et al., J Chromatogr A 2016).

The AbsoluteIDQ® Stero17 Kit is designed to help researchers to unravel the complexities of steroid hormone effects. It provides highly repro-ducible data on 17 functionally important steroid hormones in a single assay.

Key Benefi tsThe Most Accurate Steroid Hormone Analysis

Best Fit to Physiological Range• Refl ection of actual steroid hormone status• Designed to cover physiological range for the

widest range of conditions

Comprehensive Coverage• Quantifi cation of 17 steroid hormones• All fi ve steroid hormone classes covered

Ready-to-Use• All in one box: no method or reagent

development required• Implemented within one day• For multiple (U)HPLC-MS platforms

High Throughput • Only 7 minutes per sample• Up to 500 samples per week and instrument

Simple, Guided, Automated Workfl ow• Workfl ow manager guided procedure

for enhanced process safety• Automated quantifi cation and technical validation

Superior Data Quality• Quantitative results• Excellent accuracy and precision• Regularly proven in profi ciency tests

Key FeaturesQuantifi cation of 17 Steroid Hormones from all Five Classes

Instrument Platforms*

Metabolite Coverage

Kit Components

• Waters Xevo TQ-S UHPLC • Waters Xevo TQ-S micro UHPLC • SCIEX 5500 HPLC

Gluco corticoids• Cortisol• Cortisone• 11-Deoxycortisol

Progestogens• 17α-Hydroxyprogesterone (17α-OHP)• Progesterone

Mineralo corticoids• Aldosterone• Corticosterone• 11-Deoxycorticosterone (DOC)

Estrogens• Estradiol (E2)• Estrone (E1)

Androgens• Androstenedione• Androsterone• Dehydroepiandrosterone (DHEA)• Dehydroepiandrosterone sulfate (DHEA-S)• Dihydrotestosterone (DHT)• Etiocholanolone• Testosterone

Reagents & Consumables• 96-well SPE plate• Internal & calibration standards• Quality controls• Testmix

Methods & Protocols• Sample preparation protocol• Instrument-specifi c acquisition &

quantifi cation methods• System suitability test

Workfl ow Manager MetIDQ™• Process guidance• Automated technical validation• Basic statistics (optional)

* not suited for nano or micro fl ow systems

KIT

Sample Registration• Register samples• Defi ne plate layout• Generate worklist

Sample Preparation• Perform system suitability test• Prepare kit (loading of samples)

Sample Measurement• Run samples with LC-MS• Instrument-specifi c acquisition

methods are provided

Technical Validation• Perform automated sensitivity and

accuracy check• Inter-plate monitoring of QC results

and precision

Statistics• Perform tests with MetIDQ™ StatPack Module• Use statistical programs of your choice• On demand data analysis by Biocrates

Data Quantifi cation Data Evaluation & Export• Normalize data (optional)• Export results in various fi le formats

Biological Interpretation• HMDB• Consult Biocrates

for support

Workflow Manager MetIDQ™Total: 19-40 h for up to 82 samples

optional

Simple, Guided, Automated Workfl owStandardized and Automated Workfl ows Designed for High Throughput and Robustness

15 min1 h5-6 h

30 min 15 min12 - 32 h(depending on LC-MS system)

The AbsoluteIDQ® Stero17 Kit provides highly robust, quantitative data with excellent accuracy and precision, as well as reproducibility (Koal et al., J Steroid Biochem Mol Biol 2012).

The outstanding kit performance is proven in periodical profi ciency tests organized by the German Society of Clinical Chemistry and Laboratory Medicine (DGKL), demonstrated in the fi gure to the right.

Superior Data QualityProven Accuracy and Reproducibility

Figure: Mean accuracy of 7 steroid hormone levels from quaterly DGKL profi ciency tests (2011-2017) based on 48 samples in total. The orange bars indicate the acceptance range (55%-145%) and the error bars represent ±SD.

A1 2 3 4 5 6 7 8 9 10 11 12

BCDEFGH

Plate LayoutEasy to Use 96-Well Format, for up to 82 Samples

Blank: no sample, no ISTDs

AbsoluteIDQ® Stero17 Kit plate

Zero sample: no sample, only ISTDs

Sample

Internal standards (ISTDs)

7-point calibration standards

Quality control in low, medium, high concentration

Best Fit to Physiological RangeCapture the Broadest Steroid Hormone Profi le

How to Get StartedGlobal Access to High Quality Targeted Metabolomics Results

Your Laboratory Collaborating Laboratory Metabolic Phenotyping Services

You have the right instrumentation to run the kit?

Please contact us for a detailed list of require-ments and a quote.

You want to run the kit in a collaborating laboratory?

Please contact us, and we will help you to fi nd the right partner within a global network of >100 established laboratories.

You want us to measure your samples?

Biocrates can perform the full kit workfl ow, as well as additional services, with short turnaround times. Please contact us for further information.

The serum concentrations of steroid hormones vary considerably depending on age, gender, and health status. The AbsoluteIDQ® Stero17 Kit has been developed to provide highly accurate results that fi t the expected physiological ranges (see table below). For more details, please contact us or refer to the respective client note.

Steroid Hormone

Calibration Standard Calibration RangeCal 1-7 [ng/mL]Cal 1 Cal 2 Cal 3 Cal 4 Cal 5 Cal 6 Cal 7

Gluco-corticoids

Cortisol 1.0 – 1000

Cortisone 0.10 – 100

11-Deoxycortisol 0.010 – 10

Mineralo-corticoids

Aldosterone < 0.025 – 5.0

Corticosterone 0.030 – 30

DOC < 0.0075 – 15

Progestogens17α-OHP 0.050 – 50

Progesterone 0.060 – 9.0

EstrogensEstradiol < 0.005 – 20

Estrone < 0.030 – 15

Androgens

Androstenedione 0.032 – 8.0

Androsterone < 0.050 – 5.0

DHEA 0.12 – 30

DHEA-S 32 – 8000

DHT < 0.012 – 30

Etiocholanolone < 0.060 – 6.0

Testosterone 0.010 – 10

Physiological range in human serum, according to Human Metabolome Database (HMDB), May 2017

Physiological range in human serum, according to Biocrates in-house database, March 2017

< <

Physiological concentration in human serum may be below LLOQ, according to HMDB May 2017 or Biocrates in-house database, March 2017

For research use only. Not for use in diagnostic procedures.For USA and Canada: License pending

Document Nr. 35 029, V02-2019Photos: Biocrates, Fotolia, Depositphotos

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