Supplemental Figure S4. The analysis of BzS and BzR properties in vitro and in vivo. Quantitative RT-PCR of Ddit (CHOP) expression prior to (“-”) or following (“+”) 66 nM Bz (top panel) or 55 nM panobinostat (bottom panel) treatment for 24 hours. Open bars represent BzS lines and filled bars represent BzR lines. All data points shown were normalized to an untreated control mouse B cell lymphoma cell line, CH12 (not shown). Error bars represent the standard deviation of triplicate reads over each experiment, and statistical significance was determined using a two-tailed Student’s t-test (* p < 0.05; ** p < 0.005; *** p < 0.0005). B) Representative hematoxylin and eosin stained bone marrow (50X with oil), liver (20X), spleen (50X with oil) and kidney (20X) sections from moribund 595 BzS (top panels) and BzR (bottom panels) cell-injected animals receiving vehicle treatment showing the presence or absence of malignant plasma cell infiltrates.