Figure S1. Circular dichroism (CD) spectra of human telomeric i-motif DNA (ON1) and anthraquinone-modified i-motif variants (ON2-ON13) measured at 20 ºC. For sample composition see table 1.
Figure S2. CD spectra of human telomeric i-motif DNA (ON1) and anthraquinone-modified i-motif variants (ON14-ON29) measured at 20 ºC. For sample composition see table 1.
Figure S3. CD spectra of human telomeric i-motif DNA (ON1) and anthraquinone-modified i-motif variants (ON30-ON33) measured at 20 ºC. For sample composition see table 1.
25 30 35 40 45 50 55 60 65-0.1
-0.08
-0.06
-0.04
-0.02
0
0.02
0.04ON1ON2ON3ON4ON5ON6ON7ON8ON9ON10ON11ON12ON13
Temperature (ºC)
dA29
5nm
/dT 25 30 35 40 45 50 55 60 65
-0.1
0.1
0.3
0.5
0.7
0.9
1.1
ON1ON2ON3ON4ON5ON6ON7ON8ON9ON10ON11ON12ON13
Temperature (ºC)
Abs
orba
nce
Normalized UV absorption melting curves and first derivatives of human telomeric i-motif
DNA (ON1) and anthraquinone-modified i-motif variants (ON2-ON33, Fig S4-S6):
Figure S4. Normalized UV absorption melting curves (Up) and first derivatives (Down) versus temperature difference (0.5 ºC/min) from 25-65 ºC for i–motifs (ON1-ON13) at 295 nm (4 µM) in potassium buffer (100 mM KCl + 20 mM K2HPO4 (pH= 5.5) and 1 mM K2EDTA). Melting temperatures are within the uncertainty ± 0.5 °C as determined by repetitive experiments and Tm values were calculated taking an average of the two melting curves.
Figure S5. Normalized UV absorption melting curves (Up) and first derivatives (Down) versus temperature difference (0.5 ºC/min) from 25-65 ºC for i–motifs (ON1 and ON14-ON29) at 295 nm (4 µM) in potassium buffer (100 mM KCl + 20 mM K2HPO4 (pH= 5.5) and 1 mM K2EDTA). Melting temperatures are within the uncertainty ± 0.5 °C as determined by repetitive experiments and Tm values were calculated taking an average of the two melting curves.
25 30 35 40 45 50 55 60 65-0.09
-0.08
-0.07
-0.06
-0.05
-0.04
-0.03
-0.02
-0.01
0
0.01
ON1ON30ON31ON32ON33
Temperature (ºC)
dA 2
95nm
/dT
Figure S6. Normalized UV absorption melting curves (Up) and first derivatives (Down) versus temperature difference (0.5 ºC/min) from 25-65 ºC for i–motifs (ON1 and ON30-ON33) at 295 nm (4 µM) in potassium buffer (100 mM KCl + 20 mM K2HPO4 (pH= 5.5) and 1 mM K2EDTA). Melting temperatures are within the uncertainty ± 0.5 °C as determined by repetitive experiments and Tm values were calculated taking an average of the two melting curves.
Table S1. MALDI-TOF mass spectrometry data (calculated and found mass) of i-Motifs
![Acidi poliprotici H 2 SO 4 H 2 SO 4 H + + HSO 4 - i 0.1 M / / f / 0.1 M 0.1 M HSO 4 - H + + SO 4 2- i 0.1 M 0.1M / e 0.1 –x 0.1 + x x [SO 4 2- ] [H + ]](https://static.documents.pub/doc/80x56/5542eb66497959361e8d1ae4/acidi-poliprotici-h-2-so-4-h-2-so-4-h-hso-4-i-01-m-f-01-m-01-m-hso-4-h-so-4-2-i-01-m-01m-e-01-x-01-x-x-so-4-2-h-.jpg)
