Supplementary Figure S1
50
25
0
spec
ific
ap
op
tosi
s (%
)
BA doxo calculated simultaneous addition of application of apoptosis BA+doxo
by BA and doxo alone
BA alone
doxo alone
calculated addition of apoptosis induction by BA alone and doxo alone
effect of simultaneous combined application of BA+doxo
*
statisticalcomparison
JURKAT doxoR
50
25
0
spec
ific
ap
op
tosi
s (%
)
*
BA
doxoBA+doxo
Supplementary Figure S2
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
0 2 6 12 20 BA (µM)
parental
shNOXA
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
0 0,01 0,03 0,1 doxo (µM)
parental
shNOXA
A
B
Supplementary Figure S3
C
D
cIAP-2
XIAP
Survivin
GAPDH
BA doxo
--
+-
-+
++
--
+-
-+
++
24h 36h
BIM
BCL-XL
BID
BAK
BCL-2
GAPDH
BA doxo
--
+-
-+
++
--
+-
-+
++
24h 36h
Supplementary Figure S3
0 2 6 12 20 BA (µM)
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
parental
shMcl-1
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
0 0,01 0,03 0,1 doxo (µM)
parental
shMcl-1
E
F
Supplementary Figure S3
A
B
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
parental
shp53
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
parental
shp53
0 2 6 12 20 BA (µM)
0 0,01 0,03 0,1 doxo (µM)
Supplementary Figure S4
Supplementary Figure S5
50
25
0
spec
ific
ap
op
tosi
s (%
)
parental mock shp53
* *
p53
GAPDH
BA
doxoBA+doxo
NS
Supplementary Figure S6
A
B
irradiation co 12h 24h 36h 48h
p53
PUMA
GAPDH
VP16 co 12h 24h 36h 48h
p53
PUMA
GAPDH
irradiation co 12h 24h 48h
p53
PUMA
GAPDH
VP16 co 12h 24h 48h
p53
PUMA
GAPDH
75
50
25
0
C
D
spec
ific
ap
op
tosi
s (%
)
sicontrol
sip53
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
0 0,6 2 6 BA (µM)
0 0,05 0,2 0,5 doxo (µM)
siNOXA
sicontrol
sip53
siNOXA
Supplementary Figure S6
E
F
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
0 0,2 0,6 2 6 BA (µM)
Supplementary Figure S6
0 0,01 0,03 0, 1 doxo (µM)
sicontrol
sip53
siNOXA
sicontrol
sip53
siNOXA
p53
Histon H1
NOXA
GAPDH
PUMA
GAPDH
BA doxo
--
+-
-+
++
--
+-
-+
++
sicontrol sip53
BA doxo
--
+-
-+
++
--
+-
-+
++
sicontrol siNOXA
BA doxo
--
+-
-+
++
--
+-
-+
++
sicontrol siPUMA
spec
ific
ap
op
tosi
s (%
)
sicontrol sip53 siNOXA siPUMA
75
50
25
0
*
*
NS
NS
BA
doxoBA+doxo
Supplementary Figure S6
G
Supplementary Figure S6
H
1,0
0,75
0,5
0,25
0
do
xo
0 0,25 0,5 0,75 1,0BA
1,0
0,75
0,5
0,25
0
do
xo
0 0,25 0,5 0,75 1,0BA
Supplementary Figure S6
1,0
0,75
0,5
0,25
0
do
xo
0 0,25 0,5 0,75 1,0
H
BA
A
B
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
parental
shNOXA
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
parental
shp53
0 0,1 0,3 1 VCR (nM)
Supplementary Figure S7
0 0,1 0,3 1 VCR (nM)
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
parental shp53 shNOXA shPUMA
NS
*
NS
BA
VCRBA+VCR
*
Supplementary Figure S7
C
A
B
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
100
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
0 2 6 20 BA (µM)
Supplementary Figure S8
0 0,05 0,2 0,5 doxo (µM)
parental
shp53
shNOXA
parental
shp53
shNOXA
75
50
25
0
spec
ific
ap
op
tosi
s (%
)
parental shp53 shNOXA shPUMA
NS
*
NS
BA
doxoBA+doxo
*
Supplementary Figure S8
C
