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Supplemetal Fig. 1

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LKB1. -. +. pAMPK(T172). HA-AMPK. FLAG-LKB1. LKB1. -. +. pAMPK(T172). AMPK. pACC(S79). FLAG-LKB1. Supplemetal Fig. 1. B. A. C. LKB1iSP. -. +. -. -. +. -. GFPRNAi. -. -. +. -. -. +. 2DG (20 mM). -. -. -. +. +. +. pAMPK(T172). AMPK. LKB1. Supplemetal Fig. 2. - PowerPoint PPT Presentation

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Supplemetal Fig. 1 FLAG-LKB1 AMPK pAMPK(T172) pACC(S79) LKB1 - + LKB1 - + pAMPK(T172) HA-AMPK FLAG-LKB1 A B 2DG (20 mM) - + - + + - pAMPK(T172 ) AMPK LKB1 LKB1iSP GFPRNAi - - + + - - - - - + + - C

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Page 1: Supplemetal Fig. 1

Supplemetal Fig. 1

FLAG-LKB1

AMPK

pAMPK(T172)

pACC(S79)

LKB1 - +LKB1 - +

pAMPK(T172)

HA-AMPK

FLAG-LKB1

A B

2DG (20 mM) - +- + +-

pAMPK(T172)

AMPK

LKB1

LKB1iSPGFPRNAi

- -+ + --

- - - ++ -

C

Page 2: Supplemetal Fig. 1

FLAG-LKB1

autorad

LKB1 -D194A LKB1 -WT

AMPKi (M)

FLAG-LKB1

LKB1 - - + +

autorad

HA-AMPK

-

AMPK-DN

Coomassie/TSC2 fragment

autorad

-

AMPK-WT

AMPKi (M)

AMPKi (M)

B

C

A- --

- + - +

Supplemetal Fig. 2

Page 3: Supplemetal Fig. 1

Supplemetal Fig. 3

HA-S6K

pS6K1(T389)

FLAG-LKB1

LKB1 - - +Rheb WT WT L64 L64- - - -

- - ++ +

Myc-Rheb

B

HA-LKB1

S6K

pS6K(T389)

S6K

LKB1 - + Rapamycin (nM) 0

pS6K(T389)

20 40 A

- +

WT 5A-

++

-

Recommended

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