Background Objectives Materials Methods Results Conclusions Current Plans Acknowledgements

Surfaces and Particle Engineering LaboratorySurfaces and Particle Engineering Laboratory

Effect of Flow on Protein Crystallisation

Dr. Jerry Heng

Department of Chemical Engineering

Imperial College London

Background Objectives Materials Methods Results Conclusions Current Plans Acknowledgements

Surfaces and Particle Engineering LaboratorySurfaces and Particle Engineering Laboratory

Flow Cell

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Background Objectives Materials Methods Results Conclusions Current Plans Acknowledgements

Surfaces and Particle Engineering LaboratorySurfaces and Particle Engineering Laboratory

Flow Parameters

• The glass capillaries in a horizontal orientation in ice/water or embedded in a flow cell of water circulated at 0 °C are joined to the flow system at each end with 1 mm I.D. silicone tubing.

• These were joined to sections of 0.6 mm I.D./1.6 mm O.D. Teflon tubing to draw from the bottom of the protein solution container or to the Tygon pump tubing at the other end via adaptors in cases where the fit of silicone to Tygon tubing was incompatible.

• Different pump flow rates were achieved simultaneously on the 8-channel pump using different diameters of Tygon pump tubing (0.38, 0.57, 0.89, 1.09, 1.3, 1.65, 2.06, 2.79 mm I.D.) with a roller speed setting to give a calculated flow rate of 15.9 μL/min for the 1.09 mm I.D. tube.

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