Genetic Modification of Eukaryotes, Part 1
I. Transgenic Animals (GMO’s)a. What are GMO’s?
b. A Brief History of GMO animalsi. Restriction Enzymes
ii. Recombinant DNA
iii. Recombinant Bacteria
iv. The Asilomar Conference on Recombinant DNA1. Recommendations
2. Experimental Design
3. Prohibited Experiments
v. Recombinant Animal Cells
vi. Transgenic Animals (https://www.nature.com/scitable/knowledge/library/transgenic-animals-in-agriculture-105646080)
1. Common techniques used
2. Applications in Agriculturea. Enhanced nutrition
b. Enhancing milk
c. Enhancing growth rates and carcass composition
d. Enhanced animal welfare
e. Improving reproductive performance
f. Improving hair and fiber
g. Pitfalls and risks
h. Aquabounty Salmon
3. GM (Oxitec) Mosquitoeshttp://www.the-scientist.com/?articles.view/articleNo/50636/title/GM-Mosquitoes-Closer-to-Release-in-U-S-/&utm_campaign=NEWSLETTER_TS_The-Scientist-Daily_2016&utm_source=hs_email&utm_medium=email&utm_content=57389453&_hsenc=p2ANqtz-84xxaNVTqZEBU9ujNCq8yc-16jc5NhzYlcycD5RP0KGyRQkDE4w60P0evP1_eHAleK_ednPYskpAqcBQX33_mMHYq-TQ&_hsmi=57389453
II. Gene Therapy and Leber Congenital Amaurosis
a. The Gene
b. The Mouse
c. The Vector
d. The dogs
e. Human studies
