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VOLUME 52 NUMBEIR 6

JOURNAL OF BACTERIOLOGYFOUNDED IN 1916

OFFICIAL ORGAN OF THESOCIETY OF AMERICAN BACTERIOLOGISTS

Devoted to the Advancement and Dissemination of Knowledge in Regard tothe Bacteria and Other Microorganisms

EditorJ. M. SHERMANAssociate Editors

L. D. FOTHERGILL W. C. FRAziER GEORGES KNAYSI STUART MUDDJ. H. MUEMR M. J. PICKETT

Advisory EditorsG. P. Bery C. E. Clifton M. W. Jennison L. F. RettgerR. S. Breed P. R. Edwards S. A. ]Koser T. M. RiversR. E. Buchanan J. F. Enders A. G. Lochhead G. H. SmithE. J. Cameron W. A. Hagan K. F. Meyer N. R. SmithP. F. Clark B. W. Hammer G. B. Reed W. H. Taliaferro

Editorial OfficeCORNELL UNIVESITY PRESS, ITHACA, N. Y.

Contentsfor DecemberThe Application of Walker's Index of Functional Normality to a Study of Developing

Chick Embryos Infected with the Levaditi Strain of Vaccinia. RACHEL E.HOFFSTADT AND HELEN B. TRIPI................................................. 617

Two New Amino-Acid-Fermenting Bacteria, Clostridium propionicum and Diplococcusglycinophilus. B. P. CARDON AND H. A. BARKER................................ 629

A Bactericidal Detergent for Eating Utensils. ALBERT F. GUITERAS AND REBECCA L.SHAPIRO...................................................................... 635

The Amount of Enzyme Inactivation at Bacteriostatic and Bactericidal Concentrationsof Disinfectants. MARTHA HOLT ROBERTS AND OTTO RAHN...................... 639

Studies on Highly Soluble Azo Sulfonamides. C. A. LAWRENCE, H. KLINGEL, ANDG. R. GOETCHIUS.............................................................. 645

The Viability of Dried Skim-Milk Cultures of Lactobacillus bulgaricus as Affected bythe Temperature of Reconstitution. MARvIN L. SPECK AND ROBERT P. MYER......... 657

Fatty Material in Bacteria and Fungi Revealed by Staining Dried, Fixed Slide Prepa-rations. KENNETH L. BURDON................................................. 665

Disparity in Appearance of True Hansen's Bacilli and Cultured "Leprosy Bacilli"When Stained for Fat. KENNETH L. BURDON..................................... 679

Comparative Action of an Extract of Brain Tissue and Penicillin on Staphylococcusaureus Infections. LEO G. NUTINI AND SISTER EVA MARIA LYNCH................ 681

The So-called Pseudomonas vendrelli. WALTER C. TOBIE ............................ 685Factors Influencing the Morphology of Blastomyces dermatitidis. SEYMOUR LEVINE

AND Z. JOHN ORDAL............................................................ 687Stages in the Life History of Phytomonas tumefaciens. ARMIN C. BRAUN AND R. P.

ELROD ....................................................................... 695The Acclimatization of Yeast to High Concentrations of Glucose: The Subsequent

Effect upon Alcohol Tolerance. WILLIAM D. GRAY............................... 703Proceedings of Local Branches of the Society of American Bacteriologists ..... ......... 711Index of Authors ................................................................... 713Index of Subjects .................................................................. 716Contents ....................................................................... iii

INDEX OFAUTHORS

Andersen, A. A., 712Anigstein, Ludwik, 400,402Appleman, M. D., 209Aronson, Karen, 257, 501

Baker, Richard F., 411Baker, W. Howard, 149Baldwin, I. L., 555Barker, H. A., 627Baum, Sidney J., 373Beck, Oleta, 402Bennett, R. E., 400Berry, James, 711Bissell, H. D., 155Bjerknes, Clara A., 152Bohonos, Nestor, 357, 610Bondi, Amedeo, Jr., 150Bradford, William L., 404Braun, Armin C., 695Braun, Werner, 243Brewer, John H., 251Bruner, D. W., 147, 493Brydson, Lorne, 367Buchbinder, L., 152Burdon, Kenneth L., 400, 665, 679Burkholder, Paul R., 503Burris, R. H., 505Burton, Kermit A., 611

Cantino, Betty C., 311Cardon, B. P., 629Carlson, H. J., 155Carpenter, Charles M., 404Chambers, Leslie A., 179Chance, H. L., 150Chapman, George H., 151Chapman, Orren D., 237Chapman, S. S., 502Chase, Merrill W., 152Chilton, Martha, 401Choudri, R. 5., 404Christensen, W. Blake, 461Clark, Paul F., 105, 337, 467Clifton, C. E., 711Colwell, Charlotte A., 417Conn, H. J., 489Cooperman, J. M., 467Copley, Alfred L., 439Coriell, Lewis L., 179, 502Creasy, J. C., 241

Crossley, Vera M., 367Crumb, Cretyl, 258Curran, Harold R., 89, 142

Daniels, Hilda S., 351Dawson, R. C., 149Day, Elizabeth, 404DeBoer, C., 357Dietz, Catherine C., 150Dietz, Iva, 399Dimmick, Isabel, 489Donovick, Richard, 223, 502Doolittle, S. P., 601Dorrell, W. W., 555Doudoroff, M., 711Douglas, H. C., 15,153Downs, Cora M., 150,502Drake, Charles H., 153Duffy, Carl E., 151

Eagle, Harry, 81Edwards, P. R., 493Eigelsbach, Henry T., 179Eisman, Philip C., 257, 501Eklund, C. M., 150Eklund, Henning W., 1Elberg, S., 423,;513Elrod, R. P., 144,405, 695Elvehjem, C. A., 105, 467Emerson, R. L., 357, 610Engley, F. B., Jr., 241Enright, John B., 712Etchells, John L., 593Evans, Fred R:, 89,142Ewing, W. H., 404

Feiner, Rose R., 375Ferguson, Mary, 367Feustel, Irvin C., 229Findlay, G. M., 255Fisher, A. Murray, 539Flickinger, May H., 611Florman, Alfred L., 307Foltz, V. D., 150Fontaine, Thomas D., 601Foster, Jackson W., 502Foter, Milton J., 111, 449, 499Foubert, Edw. L., Jr., 153Foust, C. E., 403

713

[voL. 52

Friedemann, U., 169Frisch, Arthur, 151

Fulton, MacDonald, 401Furcolow, M. L., 150

Gailey, F. B., 119, 129Galton, Mildred M., 143Garrison, E. R., 711Gee, Lynn L., 261, 271Gerhardt, Philipp, 261, 271, 283, W5Gibby, Irvin W., 449,499Giles, Norman H., Jr., 504Gillespie, Hazel B., 257Gilman, H. L., 404Gingrich, Wendell, 403Glassman, H. N., 423Goetchius, G. R., 645Gray, William D., 703Green, D. E., 502Grubapgh, Karl K., 587Guiteras, Albert F., 635Gunderson, Millard F., 303Gunness, Marion, 195Gunsalus, I. C., 403Gunter, Shirley E., 15

Haberman, Sol, 402Hall, Elizabeth, 153Hargiss, Clarice Osterud, 71Harmon, Doralea R., 337Harris, John, 150Hayward, A. E., 51Hayward, F. W., 404Henry, B. 5., 153Henry, Jane E., 481, 527Henry, R. J., 481, 527Herndon, Eleanore, 149Hess, Mary E., 143Higgenbotham, Margaret, 400Hill, J. M., 402Hillier, James, 411Hinshaw, W. R., 349Hoffstadt, Rachel E., 617Holladay, L. L., 55Hollander, A., 169Houlihan, Ralph B., 439Howard, Isabel, 519Humfeld, Harry, 229Hungate, R. E., 153Hunter, Charles A., 149, 150Hutner, S. H., 152, 213Hutton, R. S., 51

Irons, J. V., 402Irving, George W., Jr., 601

Jarvis, F. G., 119Johnson, Elmer Jay, 141Johnson, Frank H., 257Johnson, M. J., 119, 129Johnstone, Donald B., 393Jones, Doris, 327Jones, Ivan D., 593Jones, T. Duckett, 33Juenker, Arnold P., 609

Kalter, Seymour S., 237Kaplan, A. M., 513Kauffmann, F., 152Keaton, Ruth, 403Kenner, Bernard A., 449Kidder, G. W., 503Kimmelman, Leonard J., 259, 471Kleczkowska, J., 25Klein, Morton, 269, 471Klingel, H., 645Knaysi, Georges, 487Kowal, S. F., 602

Lacy, Helen A., 401TLamanna, Carl, 1, 99Lankford, C. E., 402Lawrence, C. A., 645Lederberg, Joshua, 503LeGrow, W. R., 404Lennette, E. H., 711Levine, B. S., 150Levine, Seymour, 687Ley, Herbert L., Jr., 453Lichstein, Herman C., 105, 145, 467Lindgren, L. F., 150Little, John E., 587Lockwood, Lewis B., 581Loveless, Mary H., 259Luckiesh, Matthew, 55Luria, S. E., 152Lynch, Sister Eva Maria, 681

McCall, K. B., 105McCalla, T. M., 150McCulloch, Ernest C., 158McElroy, Olive E., 1McGann, Virginia, 431, 519McNeil, Ethel, 349Marchetta, J. A., 51Marsh, George L., 311Marsh, W. S., 501Mansell, Benedict F., 33Mayer, R. L., 257, 501-Meyer, Esther, 67, 615Meyer, Karl, 375

714 INDEX

1946]

Meyeserian, Mary, 33Mordaunt, V. Dayman, 237Morrison, Martha, 404Mudge, C. S., 712Mueller, J. Howard, 453Mueller, M. G., 166Murphy, J. N., 403Myers, Robert P., 657

Naylor, H. B., 65Nelson, -George E. N., 581Nelson, W., 502Neter, Erwin, 403Nutini, Leo G., 681

Oakberg, Eugene, 152Olitsky, Peter K., 255Olson, B. H., 129Ordal, Z. John, 67, 615, 687Osler, A. G., 152Owen, Cora Rust, 353

Pederson, C. 5., 404Penfield, R. A., 241Perlman, David, 501Pilcher, K. 5., 711Poth, E. J., 402Powell, H. M., 399Prouty, Charles C., 153

Quisno, Robert A., 111, 449,499

Rahn, Otto, 404, 612, 639Rake, Geoffrey, 223Ratcliffe, H. L., 259Reid, Roger D., 251Reilly, H. Christine, 393Reyniers, James A., 399Roberts, Lucille F., 151Roberts, Martha H., 404, 612, 639Rode, L. J., 401Roepke, R. R., 604Russell, Barbara, 150

Sako, Wallace, 403Salvin, S. B., 614Savage, G. M., 385Schatz, Albert, 327Schenone, John D., 501Scherp, Henry W., 404Schneider, Morris D., 303Schoch, Eugenia Worley. 403Schuhardt, V. T., 401, 402Schultz, Edwin W., 712Sears, 0. H., 209

Serett, Yvonne V., 257Shapiro, Rebecca L., 635Sherwood, N. P., 150Skinner, Lill R., 403Slattery, Mary P., 402Smith, P. A., 565Snyder, T. L., 241Sotier, Alfred L., 151Spaulding, Earle H., 150Speck, Marvin L., 657Spray, Robb Spalding, 141Sprince, Herbert, 501Stadtman, Thressa C., 311Stahly, Grant L., 351Stanley, Alfred R., 399Starkey, Robert L., 501Starr, Mortimer P., 187Stefaniak, J. J., 119, 129Steffen, G. I., 152Steinberg, Anita, 375Stevens, Russell B., 146Stockton, J. Richard, 227, 402Stokes, J. L., 195Strandskov, Frede, 575Stuart, C. A., 431, &19Sugarman, E. I., 711Suhrland, Leif G., 404Sulkin, S. Edward, 402Syverton, Jerome T., 404

Tatum, E. L., 504Taylor, A. H., 55Tenenberg, Dan, 150Terry, Cleo Housman, 402Tobie, Walter C., 685Traub, F. B., 169Treffers, Henry P., 502Tripi, Helen B., 617

Valley, George, 504Vander Brook, M. J., 385Van de Sand, Virginia F., 145Vaughn, Reese H., 311

Waksman, Selman A., 393Wallis, A. D., 259Waugh, M. Harriet, 257Weiser, Russell S., 71Weiss, Charles, 151Wells, J. Ralph, 149Wells, W. F., 258Wheeler, K. M., 431, 519Whiffen, Alma J., 357, 610Whitney, Dorothy M., 400, 402Wickerham, Lynferd J., 293, 611

IDWE 715

[VOL. 52

Wilkerson, Martha, 401, 402Willis, Katharyn, 402Wilson, J. K., 403, 404Wilson, P. W., 505Wise, R. I., 402

Woodruff, H. Boyd, 502Woolley, D. W., 259, 501Wyss, Orville, 227, 402, 575

Zarafonetis, Christine, 337, 402

INDEX OF SUBJECTS

Absidia glauca, induced mutations of, 504Acclimatization of yeast to high sugar con-

centration, 703Acetic acid inhibiting gram-negative bac-

teria, 353Acid-fast stain, nature of, 99Actinomycetes, antibiotic activity, 503-, frequency of antibiotics production, 357Adaptation, see Acclimatization- to streptomycin, 471Adhesion of rabbit platelets to bacteria, 439Aeration, effect on Brucella suis, 261, 271,

283,423- apparatus for laboratory experiments, 229- apparatus for large volumes of culture,

423- increasing amylase production, 351Aerobacter species with yellow pigment (title

only), 402Agar contains substance inhibiting gonococ-

cus, 453Agrobacterium, nutritive requirements, 187Air contamination, sampling devices, 55- infection, Mycobacterium tuberculosis as

indicator, 258Alanine fermentation by Clostridium pro-

pionicum, 629Alcohol tolerance of yeast, increase of, 703Allantoic fluid, infected, producing false

hemoagglutination, 307Allergy against insulin (title only), 259Amino acid composition of microorganism,

195- acid fermentation by bacteria, 629- acid needs of Streptococcus faccalis, 501Aminobenzoic acid, see p-Aminobenzoic

acidAmmonia as intermediate in N-fixation, 505- assimilable by all yeasts, 293Amylase production increased by aeration,

351Animals, germ-free, living, 399Antibacterial agents, see DisinfectantsAntibiotic action of actinomycetes, 503Antibiotics (title only), 504-, adaptation to streptomycin, 471

, antifungal streptomycin, 610, antifungal substance from Aspergillusflavus, 614

, identification by resistant variants, 501, streptin, 502

-, tomatin, 601, violacein, prodigiosin, phthiocol, 145, see also Penicillin, Streptomycin,Streptothricin

-from brain tissue, 681-from higher plants, 155- from plant juices, 587- production by actinomycetes and molds,

357Antihistaminic substances not interfering

with antigens, 257Anti-human-globulin serum (title only), 402Antiphage agents from microorganisms, 327Antirickettsial action of p-aminobenzoic

acid, 402Antiseptics, removable, 152-,see DisinfectantsArabinose oxidation by Pseudomonas, 581Arthritis, serology of rheumatoid (title

only), 259- treated with streptomycin, 399Ascorbic acid increasing viability of dry

bacteria, 565Aseptic handling of viscous materials, 373Auxanography, 503Azo sulfonamides, efficiency in vitro and in

tvio, 645Asotobacter, NH, intermediate in N-fixation,

505

Bacillus, key for identification, 400- globigii, variation in sporulation, 51- macerans, amylase production, 351- subtilis, proteinase production, 227Bacterial concentration by filtration

method, 513Bacteriophage, antibiotics against, 327-, relation to host cell, 481, 527- adsorption by resistant strains, 481- isolated by means of cationic detergents,

237

716 INDEX[

INDEX

Bacteriophage separation from bacteria byifiters, 489

- separation from culture, methods for, 25Bacteriostasis not due to enzyme inactiva-

tion, 639Bacterium tularense, electron microscopy of,

179- tularense, plate counting method, 241- tularense, survival in water, 150- tularense, treatment by chemotherapy

(title only), 502Beet molasses, citric acid fermentation, 55Bile plus indole effect on enterobacteria

(title only), 150Blastomyces dermatitidis, morphology, 687Borelia recurrentis, 401, 402Botulinum toxin isolation, 1Brain tissue contains antibiotic, 681Bromine, bactericidal action (title only),

402Brucella abortus, dissociation retarded by

serum, 243- isolation helped by penicillin, 401- sui8, concentration of cells from broth,

513- suis, continuous culture, 283- suis, method for aerated cultivation, 261,

423Brucellosis treatment with streptomycin

(title only), 404

Candida, pathogenicity for chick embryo,615

Cardiolipin antigen (title only), 400Cationic detergent disinfecting waterman

151- detergents, see Disinfectants- detergents inhibited by soap, 635- detergents used in bacteriophage isola-

tion, 237Ceepryn, see Cetyl pyridinium chlorideCell concentration by filtration method, 513Cellulose fermentation (title only), 153Cetyl pyridinium chloride, action in vitro,

- pyridinium chloride, action in vivo, 449Chick embryo abnormalities from vaccinia

virus, 617embryo contamination corrected bystreptomycin, 67

Citric acid fermentation of beet molasses,55

Clopstridium botulinum, isolation oftoain, 1- propwonicum n.sop.fermenting alanine, 629

Coagulase tests of staphylococci, 151Coliform bacteria, seeEnterobacteriaccaeColony, small, variants of bacteria, 417Continuous culture method, 283Corynebacterium acnes = Propionibacterium

a-ne8, 15Crystal violet inhibiting respiration (title

only), 150Cucumber fermentation, lactic acid bac-

teria from, 593Cysteine effect in sterilization of media

(title only), 402

Desiccated bacteria,see Dried bacteriaDiplocos glycirophiluts n. sp. fermenting

glycine, 632Disinfectant, brain tissue contains, 681Disinfectants, azosulfonamides, 645

cetyl pyridinium chloridein5vitro, 11scetyl pyridinium chloride in vivo, 449(ethyl cetab, 635

Desinhibition of gram-negative bacteria byacetic acid, 353

-,maintenance of constant phenol resist-ance, 499

-,mode of action, 639-,mode of action (title only), 502Dspenicillin plus sulfonamides, 33-,removable volatile antiseptics, 152

-, sodium fluoride fortified by NaCl, 612-, thiopyrimidines, 575- inactivating enzymes, 639Disinfection, method for invivo assays, 449- of water main by cationic detergnt, 151Dried bacteria, factors affecting viability,

209,565,657- bacteria, spray-drying preferable to

freeze-drying, 657- lactobacilli, viability, 657- Rhizobisumr,viability, 209- Serratia, viability, 565

Eating utensils, sterilization of, 635Eijkman reaction with Erwrinia, 144Electron microscope, mounting of bacteria

for, 411- microscopy of Bacterium tularense, 179Encephalitis, wester equine (title only),

150- in Califoria, 711Encephalomyelitis, related to thiamine de-

ficiency, 467Enterobacteriacai, serology (title only), 152-, swarming of, 519

1946] 717

[VOL. 52

Enterobacteriaceae decomposing tartrates,311

- differentiated by urea fermentation, 461Enterococci, enrichment media for (title

only), 150Enzyme chemistry of bacteria (title only),

602- inactivation by disinfectants, 639Erwinia, differentiation from Shigdla, 144- tracheiphila related to Shigella, 405Eryjsipelothrix rhusiopathiae from rats (title

only), 153Escherichia coli, mutations, 504- coli, small colony variants of, 417

Fat-staining method distinguishing "Myco-bacterium leprae"l from Hansen bacillus,679

Fatty acids inhibiting growth, 459-material in bacteria, staining method, 665Filters for bacteriophage not reliable, 489Folic acid deficiency and susceptibility to

poliomyelitis, 105Food poisoning by Streptococcus fasecali

(title only), 152Freezing, effect on bacteria, 71

Genetics of bacteria, 503Germ-free animals, 399Germicides, see DisinfectantsGlycine fermentation by Diplococcus gly-

cinophilus, 632Growth factor, new, required by some bac-

teria, 712- factors, streptogenin, 501- factors required by Agrobacterium, 187- factors required by protozoa, 503-factors required by rhodobacteria, 213- inhibition by analogues of metabolites

(title only), 259

Hansenula anomala, inclusions of, 487Heat activation of dried bacteria, 657Hemoagglutination, fals, from infected

allantoic fluid, 807Hemophilus pertussis disappears after strep-

tomycin treatment, 404Histoplasmin sensitivity, distribution of

(title only), 150Hydrogen sulfide formation by Shigella al-

kalescens, 143Hydrostatic pressure denaturing antibodies

and proteins, 257

Influenza epidemic of 1945-46 (title only),402

- virus, false hemoagglutination tests, 307Inhibitor for gonococcus in agar, 453Insecticides, effect on soil bacteria (title

only), 404Insulin allergy (title only), 259Intestinal bacteria agglutinated by host

serum, 257- flora affected by p-aminobenzoic acid, 400

Keratin in papillomatosis (title only), 404

Lactobacillus bulgaricus, viability of drycells, 657

Legume bacteria, see RhizobiumLeptospirosis, therapy (title only), 150Leuconostoc in Cheddar cheese (title only),

153Lipid inclusions in Hanmenula, 487-material in bacteria, staining method, 665Listerellosis (title only), 150Loeffler's serum, recipe, 141Lyophiled bacteria, see Dried bacteriaLysis of bacteria by lysozyme, 375

Maple syrup darkening, 404Marfanil, mode of action (title only), 150Mastitii, role of vitamin A (title only),

150-, staphylococcal, in the Northwest, 153Meningococcus carriers (title only), 153Metanilamide against avian malaria (title

only), 403Microbic dissociation, effect of serum, 243Micrococci, anaerobic gram-positive, 153Micrococcus lysodeikticus, cultivation, 375Milk, reconstituted, factors controlling

plate count, 711Molase, citric acid formation from, 565Molds, frequency of antibiotics production,

357Motility of Enterobacteriaceae, 519Mutations, induced, of Absidia glauca, 504- in Eacherichia coli, 504- in organism (title only), 504- to sulfonamide resistance, 152Mycelium fragmentation by blender, 385"Mycobacterium lepras" distinguished from

Hansen's bacillus, 679-tubeculosi8, counting method, 258- tuberculosis, enumeration in vivo, 259- tuberculosis, media for routine isolation,

149- tuberculosis as indicator of air infection,

258

718 INIPMU

INDEX

Neisseria gonorrhoeae inhibited by sub-stance in agar, 453

- gonorrhoeae resistance to penicillin (titleonly), 404

Nitrogen assimilation controlled by vita-mins, 293

- assimilation tests for yeasts, critique, 293- fixation, NH, as intermediate, W5- gas from nitrate by rhizobia, 403

Ocular infection, bacteriology of (titleonly), 151

Oxidase test (title only), 404

Paints, destruction by marine bacteria, 501p-Aminobenzoic acid and intestinal flora,

400- acid, antirickettsial activity, 402Paracolon type resembling Proteus and Shi-

gella, 431Pasteurella tularensis, survival in water

(title only), 150- tularensis, see also Bacterium tularensePenicillin, mode of action, 539, 639-, potentiation by enzyme inhibitors, 502

, resistance of bacterial spores, 89-action on viridans streptococci, 33-activity of the different types in vitro,

81- added for isolation of Brucella, 401- affecting cell morphology, 539- assay by reductase test, 251-formation, effect of environment, 119- formation by different strains, 129- manufacture, blending of seed, 385- manufacture, factors in, 711- -resistant gonococcus (title only), 404- sulfonamide mixtures, 33- -thiazole mixtures (title only), 402Penicillium, mechanical fragmentation by

blender, 385- notatum-chrysogenum, varying penicillin

yields, 129Pentose oxidation by Pseudomonas, 581Pertussis, see also Hemophilus,- immunity in infants (title only), 403Phage, see also Bacteriophage- resistance and carbohydrate metabolism,

527Phagocytosis depending upon temperature,

337Phenol resistance, maintenance of con-

stancy, 499Phthiocol, antibiotic, 145Phytomonas tumefaciens, life history of, 695

Pickle fermentation, lactic acid bacteriafrom, 593

Plant residues decomposed by fungi, 149Platelets of rabbit adhering to bacteria, 439Poliomyelitis and folic acid deficiency, 105- murine strain virulent after egg cultiva-

tion, 712- neutralization tests in Africa, 255Polysaccharide synthesis by bacteria (title

only), 711Powdered milk, factors controlling plate

count, 711Pressure, hydrostatic, denaturing proteins

and antibodies, 257Prodigiosin, pigment, not antibiotic, 145Propionibacterium acnes, 15Proteinase of Bacillus subtilis, 227- of Bacillus subtilis (title only), 402Protozoa, physiology of, 503Pseudomonas oxidizing pentoses, 581- vendrelli, 685

Quaternary ammonium compounds (titleonly), 712

- ammonium compounds, see Disinfectants

Reductase method for penicillin assay inbody fluids, 251

Relapsing fever, serological aspects, 402- fever from one single spirochete, 401Rhizobium, viability of dried cells, 209- producing nitrogen gas from nitrate, 403Rhodobacteria, essential elements for (title

only), 152, growth factor requirements, 213

Ribose oxidation by Pseudomonas, 581Rickettsiae and p-aminobenzoic acid, 402- of typhus in rat parasites (title only),

403

Salmonella, monophasic, for serum produc-tion, 493

-, rapid method of phase isolation, 609-, smooth "O" antigen preparation, 367

, unusual types, 401abortus-equi found in man, 147

- blegdam found in the Philippines, 146- differentiation from paracolon, 401- identification, media for, 403- phase-specific sera by monophasic

variants, 493- senegal n. sp., 349Serology of rheumatoid arthritis (title

only), 259

1946] 719

[VOL. 52

Serratia marcescens, viability of dry cells,565

Serum, phase-specific for Salmonella, 493Shigella acquiring streptomycin resistance

(title only), 259alkaleacene forms hydrogen sulfide, 143

-related serologically to Erwinia, 405variants resembling Proteu8, 431

Sodium fluoride fortified by sodiumchloride, 612

Soil structure affected by microorganisms,150

Spirochete, single individuals cause relap-sing fever, 401

Sporeformers, key for aerobic, 400Spores, resistance to penicillin, 89-, resistance to streptomycin, 142Sporulation, variation in, 51- frequency in different media, 51- medium for yeasts, 611Spotted fever and p-aminobenzoic acid, 402Stain, acid-fast, nature of, 99Star formation by Phytomonas tumefaciens,

695Starch counteracting growth inhibitor from

agar, 453- in preparation of smooth "O" Salmonella

antigens, 367Streptin, a new antibiotic, 502Streptobacillus moniliformie infection (title

only), 151Streptococcus faecali8, amino acid needs, 501- faecalis, glycolytic stimulant for, 403-faecalis food poisoning (title only), 152Streptogenin, a new growth factor, 501Streptomyces grieus, isolation of antibiotic

strains, 393- griseus, nutritional requirements, 223Streptomycin, acquired resistance by

Shigellae (title only), 259-, acquisition of resistance to, 471-, isolation of good Streptomyces strains,

393.-, potentiation by enzyme inhibitors, 502

, resistance of bacterial spores, 142- against arthritis, limitations, 399- against brucellosis (title only), 404- against contamination of chick embryos,

67- against meningitis, 711- against whooping cough (title only), 404- as antifungal antibiotic, 610- interference by reducing agents, 150--resistant strain used to identify anti-

biotics, 501

Streptothricin, toxicity of, 399- manufacture (title only), 400Sugar tolerance of yeast, induced, 703Sulfonamide resistance, mutation in, 152Swarming of Enterobacteriaceae, 519Syntrophism, 503

Tartrate decomposition by coliform bac-teria, 311

Temperature, death by law, 71- affecting rate of phagocytosis, 337Tetanus toxin potentiation by broth and

serum, 169Thiamine protecting chicks against en-

cephalomyelitis, 467Thiopyrimidines inhibiting bacteria, 575Thiouracil antagonized by uracil, 575Tomatin, antibiotic from tomato, 601Trace element requirements of rhodobac-

teria, 218Transfusion, care taken in, 399Trichinosis, serology of (title only), 151Trimethylamine oxide reduction by Er-

winia, 144Tuberculin hypersensitivity, transfer of

(title only), 152Tularemia, chemotherapy of (title only), 502Typhoid antigens administered orally, 149

Urea assimilable by all yeasts, 293- fermentation, new medium for, 303

fermentation differentiating Enterobac-teriaceae, 461

Vaccinia affecting functional normality ofchick embryos, 617

Variants, resistant to streptomycin, 471Variation, see also Mutations,- of Escherichia coli, 417Violacein, pigment and antibiotic, 145Virus diseases influenced by ether anes-

thesia (title only), 402Viruses and cancer (title only), 404Viscous materials, aseptic handling of, 373Vitamin, new bacterial, from plants, 712Vitamins, see al8o Growth factors,- controlling nitrogen assimilation, 293Volutin inclusions in Han8enula, 487

Walker index applied to vaccinia-infectedchick embryos, 617

Weed killers, effect on soil bacteria (titleonly), 404

720 INDEX:

1946] INDEX

Xylose oxidation by Pseudomona, 681

Yeast, assimilation and growth problems,711

-, nitrogen assimilation tests with, 293

721

- acclimatization to high sugar concentra-tion, 703

- manufacture on laboratory scale, 229- sporulation, medium for, 611- vitamins controlling assimilation, 293

JOURNAL

OF

BACTERIOLOGY

VOLUME 52

BALTIMORE, MD.1946