Swine Influenza Canada
John Pasick
Canadian Food Inspection Agency
National Centre for Foreign Animal Disease
Swine Influenza group technical meeting, 27-28 March 2012
OIE Headquarters, Paris
Canadian Pig Inventory 1991 - 2011
12 million pigs
Quebec
3,920,000
Ontario
2,850,800
Manitoba
2,630,000
Alberta
1,505,000
Saskatchewan
895,000
Atlantic
Canada
106,000
British
Columbia
89,000
2007
2008 2009 2010 2011 2012
January 1 2 8 8
February 3 1 4 2
March 1 2 4
April 2 3 5 4
May 3 3 4 3 4
June 1 7 3 1
July 3 2 1 5
August 1 1 1 1
September 1 3 1 5
October 1 1 5 1
November 3 5 2 4 3
December 4 3 4 1
Total 17 21 25 31 37 10
Diagnostic Submissions - Ontario
Swine Influenza Vaccines & Virological
Monitoring
• CFIA has recently simplified the procedure for
licensing influenza vaccine for swine
• Provides the opportunity for a line of products that
will contain current influenza strains
• Pfizer Animal Health has licensed a product that
would allow such vaccination
• Regular updating of vaccines critically relies on
virological monitoring of representative viruses in
various swine populations
HA
NA
PB2
PB1
PA
NP
M
NS
HA
NA
PB2
PB1
PA
NP
M
NS
HA
NA
PB2
PB1
PA
NP
M
NS
A/Sw/QC/1262080/2010
(H3N2)
A/Mink/NS/1265707/2010
(H3N2)
A/Tk/ON/FAV009/2011
(H3N2)
Quebec Nova Scotia Ontario
Swine TR H3N2 lineage
pH1N1 lineage
undetermined
Swine TR H3N2 and pH1N1 Reassortants
Phylogenetic tree of the HA gene nucleotide sequences of the swH3N2/pH1N1 strains.
Tremblay D et al. J. Clin. Microbiol. 2011;49:4386-4390
Cluster IV
Cluster V?
* *
*
TK/ON/FAV3/2011 vs
DK/MN/1979
TK/ON/FAV9/2011 vs
DK/MN/1979
TK/ON/FAV3/2011 vs TK/ON/FAV9/2011 vs
SW/ON/33853/2005 SW/ON/33853/2005
50
A/Mallard DUCK/MN/79-H3N4 QDLSENNNNT ATLCLGHHAB PNGTMVKCIT DDQIEVTNAT ELVQSSSTGK
A/SW/ON/33853/2005-H3N2 .K.PGSD.SM .........V ....L..T.. .......... .........R
A/Tk/ON/FAV-003/2011-H3N2 .K.P..D.SM .........V ....L..T.. .......... .........R
A/Sw/QC/1698-1/2009-H3N2 .K.P..D.SM .........V ....L..T.. .......... .........R
A/Sw/QC/1265553/2010-H3N2 .K.PGSD.SM .........V ....L..T.. .......... .........G
A/Tk/ON/FAV-009/2011-H3N2 .K.PGSD.SM .........V ....L..T.. .......... .........G
A/Tk/ON/FAV-010/2011-H3N2 .K.PGSD.SM .........V ....L..T.. .......... .........G
100
A/MALLARD_DUCK/MN/79-H3N4 ICNNPHKILD GRDCTLIDAL LGDPHCDAFQ DETWDLYVER STAFSNCYPY
A/SW/ON/33853/2005-H3N2 ...S..Q... .KN....... .......D.. NKE...F... ...Y......
A/Tk/ON/FAV-003/2011-H3N2 ...S..Q... .KN....... .......E.. NKE...F... ...Y.S....
A/Sw/QC/1698-1/2009-H3N2 ...S..Q... .KN....... .......E.. NKE...F... ...Y.S....
A/Sw/QC/1265553/2010-H3N2 ...S..Q... .KN....... .......D.. NKE...F... ...Y......
A/Tk/ON/FAV-009/2011-H3N2 ...S..Q... .KN....... .......D.. NKE...F... ...Y......
A/Tk/ON/FAV-010/2011-H3N2 ...S..Q... .KN....... .......D.. NKE...F... ...Y......
C1 150
A/MALLARD DUCK/MN/79-H3N4 DVPDYASLRS LVASSGTLEF ITEGFTWTGV TQNGGSGACK RGPANGFFSR
A/SW/ON/33853/05-H3N2 Y......... .......... TQ.S.N.... A.D.S.Y..R .ESV.S....
A/Tk/ON/FAV-003/2011-H3N2 Y....V.... .......... TQ.S.N.... A.D.S.R... KKSV.S....
A/Sw/QC/1698-1/2009-H3N2 Y....V.... .......... TQ.S.N.... A.D.S.Y..R .KSV.S....
A/Sw/QC/1265553/2010-H3N2 Y......... .......... TQ.S.N.... A...S.RS.R ..SD.S....
A/Tk/ON/FAV-009/2011-H3N2 Y......... .......... TQ.S.N.... A.D.S.RS.R ..SD.S....
A/Tk/ON/FAV-010/2011-H3N2 Y......... .......... TQ.S.N.... A.D.S.RS.R ..SD.S....
A A A A 200
A/MALLARD DUCK/MN/79-H3N4 LNWLTKSGSA YPVLNVTMPN NDNFDKLYVW GVHHPSTNQE QTNLYVQASG
A/SW/ON/33853/05-H3N2 ....HNLDYK ..A....... ..K.....I. .....G.DRD ..........
A/Tk/ON/FAV-003/2011-H3N2 ....HNLNYK ..A....... ..K.....I. .....G.DRD ..........
A/Sw/QC/1698-1/2009-H3N2 ....HNLDYK ..A....... ..K.....I. .....G.DRD ..........
A/Sw/QC/1265553/2010-H3N2 ....YHL.HK ..T....... ........I. .....G.DKD ..S.......
A/Tk/ON/FAV-009/2011-H3N2 ....YHL.HK ..A....... ........I. .....G.DED ..S.......
A/Tk/ON/FAV-010/2011-H3N2 ....YHL.HK ..A....... ........I. .....G.DED ..S.......
B1 B2 250
A/MALLARD DUCK/MN/79-H3N4 RVTVSTRRSQ QTIIPNIGSR PWVRGQSGRI SIYWTIVKPG DILVINSNGN
A/SW/ON/33853/05-H3N2 ......K... ..V....... .....V.SI. .......... ...L...T..
A/Tk/ON/FAV-003/2011-H3N2 K.....K... ..V.....Y. .....V.SI. .......... ...L.S.T..
A/Sw/QC/1698-1/2009-H3N2 ......K... ..V....... .....V.SI. .......... ...L...T..
A/Sw/QC/1265553/2010-H3N2 ..I...K... ..V......K .....V.SI. .......... ...L...T..
A/Tk/ON/FAV-009/2011-H3N2 ..I...K... ..V....... .....V.SI. .......... ...L.K.T..
A/Tk/ON/FAV-010/2011-H3N2 ..I...K... ..V....... .....V.SI. .......... ...L.K.T..
D D D D D 300
A/MALLARD DUCK/MN/79-H3N4 LIAPRGYFKM RTGKSSIMRS DALIDTCISE CITPNGSIPN DKPFQNVNKI
A/SW/ON/33853/05-H3N2 .........I QS........ ..P.GN.N.. .......... ........R.
A/Tk/ON/FAV-003/2011-H3N2 ........R. QS......K. ..P.GN.N.. .......... ........R.
A/Sw/QC/1698-1/2009-H3N2 ........RI QS......K. ..P.GN.N.. .......... ........R.
A/Sw/QC/1265553/2010-H3N2 .........I QS........ ..S..N.N.. .......... ........R.
A/Tk/ON/FAV-009/2011-H3N2 .........I QS........ ..S..N.N.. .......... ........R.
A/Tk/ON/FAV-010/2011-H3N2 .........I QS........ ..S..N.N.. .......... ........R.
C 328
A/MALLARD DUCK/MN/79-H3N4 TYGACPKYVK QSTLKLATGM RNVPEKQT
A/SW/ON/33853/05-H3N2 ......R... .N........ .....K..
A/Tk/ON/FAV-003/2011-H3N2 ......R... .N........ ....DK..
A/Sw/QC/1698-1/2009-H3N2 ......R... .N........ .....K..
A/Sw/QC/1265553/2010-H3N2 ......R... .N........ .....K..
A/Tk/ON/FAV-009/2011-H3N2 ......R... .N........ .....KE.
A/Tk/ON/FAV-010/2011-H3N2 ......R... .N........ .....K..
H1N1
H3N2
???
PROCINORTE Workshop on Influenza A
Virus Molecular Diagnostic Techniques
• Share experiences, information and hands-on approaches used in the molecular diagnosis and characterization of influenza A virus infections
• Identify the best practices used in molecular diagnosis with focus on sequencing – Sanger method
• Discuss methods used in virus isolation and characterization
• Identify and discuss ways in which Canada/USA/Mexico could harmonize influenza A virus molecular diagnostic methods with an emphasis on sequencing
Outcomes
• Participants compared molecular diagnostic techniques at the bench level
• Potential areas of collaboration were identified
• Of the three countries, the USA is currently putting the most effort into swine influenza virus surveillance
• Significant knowledge gaps regarding the swine influenza viruses circulating in Mexico still exist
