Session 2009-2010

FACULTY OF AGRICULTURE UNIVERSITY OF RAJSHAHI RAJSHAHI

DEPARTMENT OF GENETIC ENGINEERING AND BIOTECHNOLOGY

The courses and Curricula layout for a four-Years B.Sc (Honours) in the department of Genetic Engineering & Biotechnology is presented as below. 1. Total number of units is 40 spread over 4 years. 2. Each theory unit will be of 100 marks unit for 75 marks and unit for 50 marks. 3. The duration of examinations of the theory courses shall be 3 and 4 hours for 0.50 and 0.75 unit courses respectively. The duration of practical examination shall be 6 hours for o.50 units. For other fractions of a unit, proportionality shall be applied. Mark distributions: The Year-wise distribution of marks among the theory, practical, viva-voce, class assessment/ Field report/examination/ Tutorial/ terminal/ Class attendance, thesis/ dissertation/ project etc. shall be as follows: Each theory consists of 80% course examination and 20% tutorial examination. (i)B.Sc. Houours Part-1 Examination Nature of course Theory (including related) Practical Viva-voce Class assessment /tutorial/terminal /class attendance/ Field report/ examination, etc Total (credit courses) Units 6 2 1 0.5 9.5 Marks 600 200 100 50 950 Marks 550 250 100 50 950 Marks 725 200 50 75 1050 Marks 675 200 50 75 50 1050 Credits 24 8 4 2 38 Credits 22 10 4 2 38 Credits 30 8 2 2 42 Credits 28 8 2 2 2 42

(ii) B. Sc. Honours Part-2 Examination: Nature of course Units Theory (including related) 5.5 Practical 2.5 Viva-voce 1.0 Class assessment /tutorial/terminal /class attendance/ Field report/ 0.5 examination, etc2 Total (credit courses) 9.5 (iii) B. Sc. Honours Part-3 Examination: Nature of course Units Theory (including related) 7.25 Practical 2.0 Viva-voce 0.5 Class assessment /tutorial/terminal /class attendance/ Field report/ 0.75 examination, etc Total (credit courses) 10.5 (iv) B. Sc. Honours Part-4 Examination: Nature of course Units Theory (including related) 6.75 Practical 2.0 Viva-voce 0.5 Class assessment /tutorial/terminal /class attendance/ Field report/ 0.75 examination, etc Project/Thesis/dissertation in plant training/special practical 0.50 Total (credit courses) 10.5 1 30% of the total practical marks shall be allotted for continuous laboratory assessment.2 3

20% of the assessment marks shall be awarded for attendance in the class on the basis of the following table: Theoretical course(s) may be offered instead, it a department desires.

Table for awarding marks for attendance:

Attendance 90% and above 80% and above 70% and above 60% and above Less then 60%

Marks 10 9 8 7 0

FIRST YEARExamination: Part-I, Year: 2009

Course No

Course TitleTHEORY COURSES Honours Courses:

Marks / Unit

GEB-101 GEB -102 GEB -103 GEB -104 GEB -105 GEB -106 GEB -107 GEB -108 GEB -109 GEB -110

Introductory Biotechnology Fundamentals of Genetics Cell Biology Microbiology Molecular Biology-I RELETED Courses Fundamentals of Biochemistry Fundamentals of Botany Fundamentals of Zoology HONOURS PRACTICAL COURSES Covering courses 101 & 105 Covering courses 102 & 103 Class assessment: Tutorial / Class attendance / Field report or Excursion. (25+10+15) Viva-voce RELATED PRACTICAL COURSES Covering course 107 Covering course 108 Viva-voce

75 (Unit) 75 (Unit) 75 (Unit) 75 (Unit) 75 (Unit) 75 (Unit) 75 (Unit) 75 (Unit 50 (Unit ) 50 (Unit) 50 (Unit)

50 (Unit) 50 (Unit) 50 (Unit) 50 (Unit)950(9.5 Unit)

GEB -111 GEB -112

SECOND YEARExamination: Part-II, Year: 2010

Course No

Course TitleTHEORY COURSES Honours Courses|

Marks / Unit

GEB-201 GEB -202 GEB -203 GEB -204 GEB -205 GEB -206 GEB -207 GEB -208 GEB -209 GEB -210 GEB -211 GEB -212

Molecular Biology-II Plant Developmental Biology Animal Developmental Biology Plant Breeding-I Physiology and Biochemistry of Plants Human and Animals Physiology Bioinformatics and Computer Application RELETED Courses Food and Nutrition Biochemistry Plant Pathology PRACTICAL COURSES: Honours Covering courses 201, 203 & 206 Covering courses 202, 204 & 205 Covering courses 104 & 207 Class assessment: Tutorial / Class attendance Field report of Excursion. (25+10+15) Viva-voce RELETED COURSES

75(Unit) 75(Unit) 75(Unit) 75(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit)950 (9.5 Unit)

GEB -213 GEB -214

Covering course 106 & 208 Covering course 209 Viva-voce

THIRD YEARExamination: Part-III, Year: 2011 Course No Course TitleTHEORY COURSES : Honours Courses GEB-301 GEB -302 GEB -303 GEB -304 GEB -305 GEB -306 GEB -307 GEB -308 GEB -209 GEB-310

Marks / Unit

Recombinant DNA TechnologyPlant Cell and Tissue Culture Biotechnology for Crop Protection Animal Biotechnology Molecular and Microbial Genetics Cytogenetics Biostatistics and Experimental Design Plant Breeding-II Animal Breeding Population Genetics PRACTICAL COURSES: Honours

75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 50(Unit)

GEB -311 GEB -312 GEB -313 GEB -314

Covering Courses 301.302 & 303 Covering Courses 304.308 & 309 Covering Courses 305 & 306 Covering Courses 307 & 310 Class assessment: Tutorial / class attendance / Field report orExcursion (40+10+25)

50(Unit) 50(Unit) 50(Unit) 50(Unit) 75(Unit) 50(Unit) 1050 (10.5 Unit)

Viva-voce

FORTH YEARExamination: Part-IV, Year: 2012

Course NoGEB-401 GEB-402 GEB-403 GEB-404 GEB-405 GEB-406 GEB-407 GEB-408 GEB-409 GEB-410 GEB-411 GEB-412 GEB-413 GEB-414 GEB-415 GEB-416

Course Title

Marks/Unit75 ( Unit) 75 ( Unit) 75 ( Unit) 75 ( Unit) 75( Unit) 50 ( Unit) 75 ( Unit) 50 (Unit) 75 ( Unit) 50 (Unit) 50 (Unit) 50 (Unit) 50 (Unit) 50 (Unit) 75 (Unit) 50 (Unit) 50 (Unit) 1050 (10.5)

THEORY COURSES: Honours Courses Biomedical Science and Genetic Engineering Industrial, Pharmaceutical and Environmental Biotechnology Immunology and Immunogenetics Agricultural Biotechnology Biotechnology of Microbial Systems DNA Fingerprinting Molecular Diagnostics Enzyme and Enzyme Kinetics Cancer Biology Biometrical Genetics Biophysical Methods in Life Sciences PRACTICAL COURSES: Covering Courses 401, 403 and 408 Covering Courses 402 and 404 Covering Courses 405 and 406 Covering Courses 407, 409 & 410 Class Assessment: Tutorial / Class attendance / and Field report of Excursion (40+10+25)

Project Paper / Thesis / Dissertation Viva voce

FIRST YEARExamination: Part-I, Year: 2009 Course No Course TitleTHEORY COURSES Honours Courses:

Full Marks / Unit

GEB-101 GEB -102 GEB -103 GEB -104 GEB -105 GEB -106 GEB -107 GEB -108 GEB -109 GEB -110

Introductory Biotechnology Fundamentals of Genetics Cell Biology and Cytology Microbiology Basic Molecular Biology-I RELETED Courses Fundamentals of Biochemistry Fundamentals of Botany Fundamentals of Zoology HONOURSE PRACTICAL COURSES Covering courses 101 & 105 Covering courses 102 & 103 Class assessment: Tutorial / Class attendance / Field report or Excursion. (25+10+15)

75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 50(Unit ) 50(Unit) 50(Unit)

Viva-voceGEB -111 GEB -112 RELETED PRACTICAL COURSES Covering course 107 Covering course 108

50(Unit) 50(Unit) 50(Unit) 50(Unit)950 (9.5 Unit)

Viva-voce

GEB-101 Introductory Biotechnology

Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. 2.

Time: 4 Hours

Introduction: Definition, old and new biotechnology and interdisciplinary activity, scope and importance. Commercial potentiality. Plant Biotechnology: Introduction, origin, history, laboratory organization, nutrition media. Sterilization type of culture. Large scale culture. Advantages and limitations of different types of culture. Animal Biotechnology: Introduction, history of organ culture, techniques, advantages, limitations and application. Introduction to cell culture, substrate, culture media, preparation of substrate and media, isolation of explants and desegregation of explants.Recombinant DNA technology: Definition, restriction endonucleases, modifications of cut ends. Steps in gene cloning, isolation of desired gene, uses of plasmid and marker genes. Gene transfer methods, Advantage and disadvantage of Agrobacterium mediated and biolistic gene transfer method.

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Biotechnology and health care: Introduction, disease prevention, disease diagnosis, disease treatment, gene therapy and forensic medicine. General and industrial microbiology: Historical landmarks, isolation and screening of microorganisms, inoculums development and application. Enzyme technology: Fermentation, cheese production and use of enzyme in food industry. Fuel and environment biotechnology: Introduction, types and uses of fuel. Concept of bio-fuel and biogas and their uses. Biotechnology in pollution control. Biotechnology in Agricultural Science. Biotechnology in pollution control.

Book References:Waston, J, D., Tooze, J. and Kurtz, D.T. 1983, Recombinant DNA. A short course, scientific American Books, New york. Drlica, K. 1984, Understanding DNA and Gene Cloning A Guide for the Curious, John Wiely & Sons, New York. Steven, P. 1984, Biotechnology-A New Industrial Revolution. George Braziller Inc. USA. Antebi E. and Fishiock D. 1986, Biotechnology. The Mit Press, USA. Marx J.L. 1989, A Revolution in Biotechnology Cambridge Univ. Press, UK. Old, Principles of Gene Manipulation and Introduction to Genetic Engineering. 3rd Ed. Koshland, Biotechnology. Smith. 1996, Biotechnology. Rehm. 1986. Biotechnology. Brown. 1987, Introduction to Biotechnology. Kingsman and Kingsman.1988, Genetic Engineering. B.D. Singh. Biotechnology. Expanding Horizons. G. R. Chhatwal.1998, Textbook of Biotechnology. P. K. Gupta. The Elements of Biotechnology. P. R. Trived and K. N. Sudarshan: Environment and natural resources conservation. M. M. Ranga. Animal Biotechnology.

GEB-102 Fundamentals of Genetics

Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. 2. 3 4. 5. 6. 7. 8. 9. 10 11 . 12 . 13 . 14 . 15 . 16 . 17 . Historical background of Genetics Mendel and his predecessors.

Time: 4 Hours

Mendels Law of Segregation and Law of Independent Segregation of alleles. Probability and chi-square test. Physical basis of heredity: Evidence for the chromosomal theory of inheritance. Gene symbols: Dominance and lethal gene action. Gene Interaction: Types of gene interaction and genetic explanation. Linkage, crossing over and chromosome map. Sex determination in plants & animals. Sex linkage, sex linked inheritance, sex influenced & sex limited traits. Multiple alleles. Inheritance of quantitative characters. Hardy- Weinberg law and population. Mutation: characteristics, classification, induction and detection. Cytoplasmic inheritance. Male sterility: Types and genetic control. Pedigree analysis, twins, human traits, disorders due to mutant genes.

Book References:Snustad, D.P. Simmons, M.J. and Jenkins J.B. 1997. Principles of Genetics, Jacaranda/Wiely publishers. Klung, W.S. and Cummings, M.R. 1980, Concepts of Genetics. Scott, Foresman and Company USA. Waston, J.D. and Hopkins, A.M., Roberts, J.W., Steitz, J.A. and Weiner, A.M. 1988. Molecular Biology of the Gene. Benjamin, Cummings, Scientific Publishing. Menlo Park, California. Lewin, B. Genes, VII., Oxford University Press, seventh, Editions. A.V.S.S Sambamurty. Genetics 2nd Edition. Narosa Publishing House, New Delhi. B. D. Singh: Fundamentals of Genetics.

Strickberger: Fundamentals of Genetics. Verma and Agarwal. Genetics .

GEB-103 Cell Biology Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. A brief introduction: Historical background of cell biology. Concept of life and living process, the identifying characteristics of a living matter. Concept, size and structure of prokaryotic and eukaryotic cell and their differences. Cell theory: Modern techniques of study of the cell fractionation. 2. Cell Membrane: Physical and chemical structure of cell membrane. Its organization and function. Membrane models: Danielli-Davson, Robertson, Nicholson-Singer models. Lipid bi-layer and functions of cell membranes. Membrane Transport: ways to move molecules across membrane Carrier proteins: Lon channels: Cell signaling. 3. Structural basis of the cell: Cytoskeleton, microtubules, micro-filaments and intermediate filaments. 4. Cytoplasm and Cytoplasmic Organelles: Cytoplasmic matrix, ribosomes vacuolar system and microsomes, mitochondria and chloroplasts, endoplasmic reticulum. golgi complex with their ultra structure and function. lysosomes. peroxisome and glyoxysomes. 5. Protein folding and structure: Primary, secondary and tertiary structure. 6. Physical and Chemical structure of Chromosomes: Structure of chromatin, nucleosome shape and organization. Higher order structure of chromatin, organization of chromosome. Centromere, telomere, nucleosome and its organization in eukaryotic chromosome. 7. Cell division: Cell cycle, Mitosis, Meiosis and their biological significance.

Book References:Alberts. et al, 2002, Molecular Biology of the cell. 4th edition. Alberts. et al, 2004, Essential Cell Biology, 2nd edition. Verma. P.S. and Agarwal V.K. 1974, Cell Biology, Genetics, Molecular Biology and Evolution. S.Chand & Company Ltd. Ram Nagar, New Delhi- 110055 Snyder. L.H. and David. P. R. 1957, The Principles of Heredity. D. C. Health and company, Boston. Avers C.J. 1980, Genetics D. Van Nostrand Company, New York, Cincinnati, Toronto, London Melbourne. Strickberger M.W. 1995, Genetics. Prentice-Hall of India Private Ltd. New Delhi-110001. Verma. P.S & Agarwal. V.K. 1975, Genetics. S. Chard and Company Ltd, Ram Nagar, New Delhi- 110055 Power. C.B. 1997, Cell Biology. Himalaya Publishing House, Bombay-400004. Molecular Cell Biology. H.S. Bhamrah. Sowanson. Cytogenetics. M.S. Sominath. Cell Biology. Bruce Alberts., D. Bray. J. Lewis, M. Raff. K. Roberts, J. D. Watson. Molecular Biology of the Cell. Lehninger. A. Principles of Biochemistry, Arora, M. Molecular Biology. De Robertis, E D. P. and De Robertis, E.M.F. Cell and Molecular Biology. Hall J.L.Flowers, T. J. and Roberts.R.M. Plant Cell Structure and Metabolism Verma. P. S. and Agarwal V.K. Cytology. Verma. P. S. Cell Biology.

Gerald Karp. Cell and Molecular Biology Concepts and Experiments. S. C. Rastogi. Cell Biology. New Delhi, Tata Mc Graw hill pub, Com, Ltd. David E. Sadova. Cell Biology Organelle Structure & Function. Boston, Jones & Bartlet Publication.

GEB-104 Microbiology Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. Introduction and scope of microbiology: Characteristic features of prion, viroids, virus, rikettsia. chlamydae, mycoplasma, bacteria, algae and fungi. Importance of the microbes (biological and industrial), scope of microbiology. 2. The viruses: Morphology, structure and component, replication (lytic and lysogenic cycles). Bacteriophages. Virus of animal and plant. How an animal virus gets into and out of its host cell. A brief account of poliovirus, Corona virus, AIDS virus, SARS virus. 3. Bacteria: Morphology, size, shape and arrangement of bacterial cell; Internal and external structure. How bacteria sense and swim, nutritional requirements and nutritional types of bacteria. 4. Actinomycetes: Characteristics, size, shape, reproduction, distribution, classification and importance. 5. Fungi: Molds and Yeast, distinguishing characteristics, cultivation, some fungi of special interest. 6. Control of microorganisms: Control of microorganisms by physical and chemical agents, characteristics of an ideal anti-microbial agent, major groups of anti-microbial agents. 7. Microbes and diseases: Natural resistance, pathogenecity and virulence, microbial toxins, transmission and preservation of common infectious diseases: cholera tuberculosis, tetanus and AIDS. Book References:Brock. T. D. Biology of Microoranisms. Pelzer, M.J.Kreig, C.N.R. Microbiology. Schlegel. G. General Microbiology. Tortora, Fanke and Case. Microbiology An Introduction. Dube. H. C. A Bacteria and Viruses, textbook of Fungi. Snustad, D.P. Sinnons, M.J. and Jenkns J.B. 1997. Principles of Genetics. Jacaranda, Wiely Publishers. P. Chakraborty. A Text book of Microbiology. A.K. Agarwal. Industriul Microbiology.

GEB-105 Molecular Biology - I Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. Nucleic acids: Definition structural features and types of nucleic acids; The Watson and Crick model of DNA structure; Chargaff`s Base pairing rule; cellular RNAs; Evidence for DNA and RNA as genetic material; Chemical and enzymatic hydrolysis of nucleic acids; physical and chemical properties of DNA (Tm value, Cot values, hybridization, denaturation and renaturation). The Super coiling of DNA. 2. Structure of a gene, their promoters and regulatory elements (TATA box, CAAT box etc.) Molecular concept of gene. Gene clusters and repeats, heat shock genes. 3. Replication of DNA: Mode of replication; experimental findings of Meselson and Stahl and of Taylor and Cairns; Mechanism of replication; enzymes of DNA replication and its control in prokaryotic and eukaryotic system. 4. Transcription: Prokaryotic and eukaryotic RNA polymerases, mechanism of transcription,transcription initiation complex, inhibition and regulation of transcription, promoters, enhancers, terminators, reverse transcription, Post transcriptional modifications of proteins: Mechanism of RNA splicing and RNA Processing, mRNA structure and its relation to its stability. 5. Genetic code and Translation: Evidences and Properties of genetic code. Structure and functions of ribosomes. Structure and functions of tRNA; activation initiation, elongation and termination of protein biosynthesis and their control.Book References: Brock, T.D. and Madigan M.T. 1997, Biology of Micro organisms, Prentice hall International. Tortora, Funke and Case. 1997, Microbiology An Introduction, Addison Wesley Longman Inc. Atlas R. M. 1997. Principles of Microbiology 2nd Ed. W.C. Brown Publishers, Pelezer & Reid, Microbiology. Readings from Scientific American. Molecules of life. W.H. Freeman and Company, New york. Darnell J., Lodish H. and Baltimore D.1986 Molecular Cell Biology. W. H. Freeman and Company, New York. Stryer L. Biochcmistry. W.H. Freeman and Company, New York. Alberts, B.Bray, D. Lewis J. 1989, Molecular Biology of the Cell. Garland Publishing Inc, New york. Stryer L. 1989, Molecular Design of Life. W.H. Freeman and Company, New York. Voet & Voet. 1991, Biochemistry. Lehninger, Nelson, Cox, 1990, Principles of Biochemistry. Gunthes S., Stent Richard Calendar Molicular Genetics An Introductory Narrative 2nd Edition. James D. Watson, Tania A. Baker, Stephen P Bell, Alexander Gann. Molecular Biology of the Gene.

GEB-106 Fundamentals of Biochemistry Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. 2. Important organic constituents of plants and animals. Carbohydrate Occurrence, definition, classification, physical and chemical properties. Stereoisomerism. Chemistry of monosaccharides, disaccharides and polysaccharides with special reference to starch, cellulose and cell wall polysaccharides. Colour reactions of carbohydrates. Protein Definition, classification, physical and chemical properties. Amino acid composition of protein and peptides, hydrolysis of Protein, reactions of amino acids. Amino acids as ampholytes, pK value, Isoelectric Point (PI). Protein structure: Protein domains: Protein sorting and intracellular compartments. Vesicular transport: protein modifications in the secretory pathway, protein and other molecular movement into cell. Plant protein-leaf. Seed and cereal Protein Deficient of amino acids in plant proteins. Biological value of animal and plant proteins. Lipid Definition, classification, chemical and physical properties. Fatty acid composition of fats. Chemical reactions of fatty acids. Edible oils and their characteristic, fatty acid composition. Characterization of fats, Oils and waxes, phospholipids with special reference to lecithin and cephalic. Nucleic acid Occurrence, composition and classification. Chemical and physical properties. Important functions of nucleic acids. Enzyme Definition, classification and chemical nature of enzymes. Concept of coenzymes and prosthetic groups. Mode of action of enzymes. Factors affecting enzymatic reactions. Time: 4 Hours

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Book References:Albanese. A. A. 1980, Principles of Biochemistry. Worth Pub. Philadelphia. Burton. B. T. 1976, Human Nutrition, Tata Mc Graw-Hill, New Delhi. Conn.E.E. and Stampt. P.K. 1984, Outlines of Biochemistry. John Wiley and Sons. New York. Clark. W. H. 1977, Experimental Biochemistry. Freeman and Company. Lehninger. A.I. 1980, Principles of Biochemistry. Worth Pub. Philadelphia. Merts and Parter. Laboratory Experiments in Biochemistry, Burgens Pub. Co. Minneapolis. Street 1967, Plant Metabolism, Pergam Press, London. Lehninger, Albert L. 1978, Biochemistry. M/S, Wort. Publishers Inc, New York. Lehninger, Albert L. 1978, Principles of Biochemistry. M/S Worth Publishers Inc, New York. Mathews & Van Holde 2nd Ed. Biochemistry, Benjamin, Cummings Pub. Co. Stryer, L. 4th Ed, Biochemistry. Rawn, 1989, Biochemistry. Voet & Voet, 1991, Biochemistry. Edward Staunton West, Wilbert R. Todd, How ward S. Manson. Text Book of Biochemistry. New Delhi, Oxford & IBH Pub. Co. Pvt. Ltd. Bahl & Bahl, 1984. Advanced organic Chemistry. Dev. A.C. 1996, Fundamentals of Biochemistry. Selim Reza. 2007, Medical Biochemistry.

GEB-107 Fundamentals of Botany Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. 2.

Time: 4 Hours

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Introduction: Introductory botany, branches and scope of botany, origin of living system, chemical and biological evolution of life. Biological diversities: diversities in plant kingdom, causes, importance and conservation. Systemic botany: Taxonomy: definition, objectives and uses of taxonomy, units, systems and basis of classification, naming of plant, classification of plant, systemic study and economic importance of the following families of angiosperms: Gramineae, Leguminosae, Orchidaceae and Verbenaceae. Practical implications of systemic study of families in biotechnology. Life cycle of Arabidopsis thaliana. Economic botany: Study of angiospermic plants as source of cereal, fiber, medicine, oil, beverage, rubber, sugars and narcotics. Anatomy Cell: Ultra structure and function of the components of a typical plant cell, tissue and tissue systems, compact and classification characteristic features, functions and distribution of different types of plant tissues, importance of studying tissue and tissue system in biotechnology. Embryology:Megasporogenesis,megametogenesis,microsporogenesis, icrogametogenesis, types of embryo sac and their development, pollination and fertilization. Ecology: Plant Ecosystem:(a) Ecological features of hydrophytes. mesophytes, xerophytes and halophytes (mangrove). (b) Fresh water ecology, food chain and food webs. (c) Green house effects, green house gases. Ozone depletion and CFC use in Bangladesh. Biodiversity: Introducing biodiversity, aims and objectives, types of biodiversity, and ecosystem stability, threatened biodiversity, causes of loss of biodiversity, need and methods of biodiversity Conservation.P. Maheshwari. An introduction to Embryology of Angiosperm. E. Lawrence, Mettler, G. Thomas. Population Genetics and Evolution. A. Fahn. Plant Anatomy. G.H.M. Lawrence. Taxonomy of Vascular Plants. Concepts in Biology. D. Balasubramanian, C.F.A. Bryce, K,.Dharanlingam, J. Green Kunthala Jayaraman 2005. P.K. Garg. 1990, Dictionary of Botany.Academic Publishers. C. Starr and Caggart R.1989, Biology-The Unity and Diversity of Life, Wardsworth Pub Company, USA. P.H. Raven, and Johnson G.B. 1986, Biology, Times Mirror/Mopsby College Pub. Ltd. St. Louis USA. D. Attenborough. 1979, Life on Earth. S.C. Datta.1988, Systematic Botany. Wiley Eastern limited. N.S. Subrahmanyam.1997, Subrahmanyam`s Botany Question Bank Vikas Pub. House P.L. F. Albert Hill. Economic Botany 1989,Tata Mc.Graw Hill, Pub. Com. Ltd. Esau. Plant Anatomy1980, Wily Eastern Ltd. B.P. Pandey. Economic Botany. 1995, S. Chand & Com. Ltd. George Usher. 1992, A Dictionary of Botany. CBS Publishers. B.P. Pandey. 1995, Modern Practical Botany. S.Chand & Company. H.C. Dube. 1978, A Textbook of fungi, Bacteria and Viruses.. Vikas Pub. House.Pvt.Ltd. F. Albert Hill, O.P. Sharma. 1996. Economic Botany. Tata Mc Graw Hill Pub. Com. Limited. B.R. Vashishta. 1986, Botany For Degree students Part-11 Fungi.. S.Chand. P.C. Kotwal, sujoy Banerjee. Biodiversity Conservation in Managed Forests and Protected Areas. Agro Botanica. K.C. Agrawal 1998, Biodiversity. Agro Botanica. A. Mackenzie, A. S. Ball, S.R. Virdee. 1998, Instant notes in Ecology. Viva books Pvt Ltd. B.P. Pandey. 1998, Plant Anatomy. S. Chand & Com Ltd. R.S. Shukla, P.S. Chandel. 1994, Plant Ecology and Soil Science. S. Chand & Com. Ltd. S.K. Mukerjee. 1984, College Botany. New Central book Agency. P. Maheshwari. 1950. An Introduction to the Embryology of Angiosperms. Mc Graw. Hill. R.S. Shukla, P.S. chandel. 1998. Plant Ecology. S. Chand & Com Ltd. A.V.S.S. Sammbanurty N.S.S. Subrahmanyam. 1998, A Textbook of Modern Economic Botany. CBS Publishers & Dis. C.J. Alexopoulas C.W. Mims, M. Blackwell. 1996, Introductory Mycology. John Wily & Sons. E. P. Odum. Ecology. 1997. Oxford & IBH Pub. K.Purshotam. 2000, Microbiology Questions and Answers. Wheeler publishing.

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Book References:

GEB-108 Fundamentals of ZoologyFull Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1.

Introduction to Animal Kingdom: Basis of classification, classification of animal kingdom upto order, study of the following. Animals Paramecium, Scypha, Jellyfish, Balanoglossus, Ascidia, Labeo, Lizard, Pegion, Guineapig, Prawn, Bee, Spider and Peripatus.

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Embryology: Gametogenesis, egg type and cleavage. Blastulation, Gastrulation. Organogenesis embryonic development of any mammal. Anatomy: General organization of systems and their functions. Evolution: Evidences, theories with modification, evolutionary history of man evolutionary changes of organism along with their organ system. Ecology: Environment and its components, concept of ecosystem, energy flow, biogeochemical cycle, habitat, adaptation and adaptability. Environmental pollution: conservation of environment with special emphasis to biological resources, zoogeographical regions.

Improtant Pest, their nature extent of damage of following: a. Rice: Scirpophaga incettulus, Dicladispa armigera, Leptocprisa acuta Nepha tetrix nigropictus and Spodoptera maurita. Sugarcane: Scirpophaga excerpthalis, Chilo tumidicaostalis and Odontotermis obsecus Jute: Spiosoma obiqua, Apion corchori and Anomis sabulifera Pulses: Hellothis armigera and Pulse bettle Stored Product: Tribolium castaneum, Sitophilus orygae and Corcyra cephalonica Book References:Ganguly. B. B. Sinha. A. K. and Adhikari. S. 1987. Volume 1 & 11 Biology of Animals. New Central Book Agency 8 /1 Chintumoni Das Lane: Calcutta 700009 India.. Grove. A.J. and Newell. G.E. 1994. Animal Biology. Universal Book Stall. New Delhi. Snodgrass. R.E. 1994. Principles of Insect Morphology. CBS Publishers and Distributors. 485. Jain Bhawan. Bhola Nath Nagar. Shahdara. Delhi-10032. Richards O. W. and Davies R. G. 1993. Volume: General Text Book of Entomomoly. BI Publications Pvt. ltd. New Delhi. Richards O. W. and Davies R. G. 1993. Volume 2: General Text Book of Entomomoly. BI Publications Pvt. Ltd. New Delhi. Kotpal : Modern Textbook of Zoology (Invertebrates) Kotpal : Modern Textbook of Zoology (Vertebrates)

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SECOND YEAR Examination: Part-II, Year: 2010

Course No.

Course Title THEORY COURSES Honours Courses| Molecular Biology-II Plant Developmental Biology Animal Developmental Biology Plant Breeding-1 Physiology and Biochemistry of Plants Human and Animals Physiology Bioinformatics and Computer Application RELETED Courses Food and Nutrition Biochemistry Plant Pathology PRACTICAL COURSES: Honours Covering courses 201, 203 & 206 Covering courses 202,204 & 205 Covering courses 104 & 207 Class assessment: Tutorial/Class attendance/Field report of Excursion (25+10+15) Viva-voce RELETED COURSES Covering course 106 & 208 Covering course 209 Viva-voce

Marks / Unit

GEB-201 GEB -202 GEB -203 GEB -204 GEB -205 GEB -206 GEB -207 GEB -208 GEB -209 GEB -210 GEB -211 GEB -212

75(Unit) 75(Unit) 75(Unit) 75(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit)

50(Unit) 50(Unit) 50(Unit) 50(Unit)950(9.5 Unit)

GEB -213 GEB -214

GEB-201 Molecular Biology-II Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. 2. Time: 4 Hours

Regulation of gene expression in prokaryotes: Principles of transcriptional regulation. Lac and trp operon inducible and repressible systems, positive and negative control. Regulation of gene expression in Eukaryotes: Transcription factors. Expression of

specific genes: Histone genes Globin genes, heat shock genes, leghemoglobin genes, genes for storage proteins of legumes and cereals, possible role of middle repetitive DNA in control of gene expression, Britten-Davidson model Genes for ribosomal RNA. 3. Mutation and repair of DNA: Type of mutation, spontaneous and induced mutation. Physical and chemical mutagens. Molecular basis of mutation, in vitro mutagenesis, sitedirected mutagenesis, transposons and insertional elements. Alteration and repair of DNA: Mechanism, rate and its measurement. Molecular biology of organelles: Genomes of mitochondria and plastid-interaction with nucleus. RuBisCO a case study of duel control of its synthesis by nucleus as well as plastome. Restriction modification enzymes used in recombinant DNA technology. Basic techniques in Molecular Biology (i) Extraction of DNA and RNA. Visualization of DNA using Gel electrophoresis. (ii) Polymerase chain reaction, Key considerations for designing primers. Optimization of different steps. (iii) Basic aspects of plasmid cloning vector, processing of PCR product and Cloning vector using restriction enzymes, ligation of PCR product into cloning vector. (iv) Transformation: Transferring recombinant DNA into E-coli-Chemical induction and electroporation.

4. 5.

6. 7.

Book References:Darnell, J., Lodish H. and Baltimore, 1986, Molecular Cell Biology. D.W.H. Freeman and Company, New York. Alberts B. Fray, D. Lewis, J., 1989, Molecular Biology of the Cell. Garland Publishing, Inc. New York. Gurthew S. and Richard S. Molecular Genetics-An Introduction Narrative. Karp G. Cell and Molecular Biology-Concepts and Experiments. Hancock J. T. and Pallister C. J. Molecular Genetics. Srivastava S., Srivastava P. S. and Tiwary B. N.Molecular Biology and Biotechnology.

GEB-202 Plant Developmental Biology Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1.

Plant life cycle: Gametogenesis, pollen tubes growth and guidance, double fertilization.

2. 3. 4. 5. 6. 7. 8. 9.

Embryogenesis: Establishment of meristem and maintenance. Patterning in early embryos: Mutations affecting pattern formation. Post-embryonic pattern formation: Cell lineages, cell fate, clonal analysis, lack of a germline. Photomorphogenesis. Phase change (juvenile to adult) and changes in meristem identity. Flower development: Organ identity and patterning, leaf development, root development, genes involved in development of different plant parts. Impact of plant development on plant genetics: Plant genetic model systems. Forward genetics entree: Mutagenesis-chemical, physical and T-DNA. Transposon mutagenesis. Analyzing mutations: mapping complementation.

Book References:Balinsky, B.I. 1981. An Introduction to Embryology. 5th ed. W.B. Sunders Co. West Washington Square, Philadelphia. Bodemer, C.W. 1968. Modern Embryology, Holt, Reinhart Winston Inc. NY. Chicago. Enerjee, G.C. 1987, A text book of animal Husbandry, 6th ed. Oxford and IBH pub. Co. NY. Delhi, Calcutta & Mumbai. Dalton, D.C. 1987, An Introduction to Practical Animal Breeding.. English Language Book Society Collins. Gordon, I. 1983, Controlled Breeding in Farm Animals. Paragon Press. Oxford. NY & Sydney. Hafez, E.S.W. 1987, Reproduction in Farm Animals. 5th ed Lea & Febigar, Philadelphia. S. Narayanswamy 1997, Plant Cell and Tissue CultureTata Mc Graw-Hill Publishing Company Ltd. New Delhi Rajdan. Introduction to Tissue Culture.

GEB-203 Animal Developmental Biology Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. Key concept. Development events and differential gene expression. Developmental Geneticsapproaches & techniques. Cell fate determination in C. elegans and Zebrafish. 2. 3. Early embryonic development: Gemetogenesis. Fertilization. Cleavage I, Cleavage II, Gastrulation I, Gastrulation II. Axial patterning: Axis formation in amphibians. Anterior/posterior patterning in amphibians. Anterior/posterior patterning in Drosophila (material effect genes). Anterior/posterior patterning in Drosophila (segmentation genes). Hox genes and dorsal ventral patterning. Early mammalian development. Left-right patterning, early development in zebrafish. 4. Later embryonic developments. Patterning the central nervous system. Ectoderm-eye-development, Ectoderm-epidermis and hair development. Ectoderm-neural crest development, Ectoderm-tooth development and axon guidance. Mesoderm-somites formation. Mesoderm-muscle and bone development, Mesoderm-Kidney development. Mesoderm-heart and vessels development. Mesoderm-limb formation: Endoderm; Heamatopoiesis. 5. Post-embryonic development. Sex determination-mammals, Sex determination (Drosophila) and X-inactivation, Regeneration, Environmental Regulation of development, Aging infertility and the nature of human disease, Cancer as a disease of development. Book References:Grove. A.J. and Newell. G.E. 1994, Animal Biology. Universal Book Stall. New Delhi. Leon.W. Browder: BRO Developmental Biology, Saunders College, Philadelphia. Philip Grant. Holt, Rinehart, Winston.Biology of Developing System. Gilbert S.F., Sinauer Developmental Biology, Associates Inc. Publishers Sunderland, Massachusetts. Berill, N.J. and Karp G.Development. Davidson E.H. 1968. Gene Activity in early development, New York, Academic press. Charles W., Bodemar, 1968. Holt Rinehart and Unwin, Modern embryology, London. Jack Cohen 1967, Living embryos, Pergamon press. Ganguly. B. B. Sinha. A. K. and Adhikari. S. 1987, Biology of Animals. Volume I & II New Central Book Agency. 8 /1 Chintumoni Das Lane, Calcutta- 700009 India. Kotpal . Modern Textbook of Zoology (Invertebrates). Kotpal . Modern Textbook of Zoology (Vertebrates).

Time: 4 Hours

GEB-204 Plant Breeding- I Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10 . 11 . 12 . 13 . 14 . 15 . 16 . 17 . 18 . 19 . 20 . 21 . Objectives and role of plant breeding Genetics in crop improvement. Crop genetic resources. Center of diversity and gene bank Origin of cultivated crop: Wheat. Mode of reproduction and significance in crop improvement. System of mating and role in plant breeding. Incompatibility and male sterility in crop improvement. Component of genetic variation and uses in crop improvement. Out lines of breeding methods. Plant Introduction. Pure line selection. Mass and progeny selection. Pedigree method of selection. Bulk method & modified bulk method of selection. Backcross method of selection. Recurrent selection method. Open pollinated, synthetic and composite variety. Construction of hybrid maize. Clone & clonal selection. Experimental design & Plant breeding. Time: 4 Hours

Book References:R.S. Shukla. Cytogenetics, Evolution and Plant Breeding.

J.R. Sharma. Principles of Plant Breeding. B.D. Singh. Plant Breeding. R.K. Singh & R. K. Singh: Genetics and Plant Breeding. Pirchner. Population Genetics in Animal Breeding.

GEB-205 Physiology and Biochemistry of Plants Full Marks: Theory 40 + Tutorial 10 = 50 (1/2 Unit) 1. 2. 3. 4. Time: 3 Hours

Introduction: The plant body and the typical plant cell-An overview of general structure and function, chemical properties of plant cells. Plant-water relationship: Osmosis and imbibitions, diffusion, transpiration, stomatal opening and closing. Mineral nutrition: Essential elements and their function, deficiency symptoms of major elements, uptake of mineral and their transport. Photosynthesis: Definition, historical background, location of photosynthesis, photosynthetic pigments, hill reaction, Mechanism of photosynthesis, C3, C4 and CAM plants, bacterial photosynthesis. Respiration: Definition, types of respiration, respiratory quotient, mechanism of respiration, fermentation, glyoxalic acid cycle, hexose monophosphate shunt, photorespiration. Lipid metabolism: Chemical composition of lipids, Biosynthesis and degradation of lipids, Significance and mobilization of fat reserve for growth. Nitrogen metabolism: Uptake and assimilation of nitrate, assimilation of ammonia, Nitrogen fixation- symbiotic and asymbiotic, the nif genes. Plant growth regulators: Biosynthesis analysis and physiological effects of auxins, gibberellins, cytokines, abscisic acid and ethylene. Current ideas of action of hormones.

5. 6. 7. 8.

9.

Light and plant life: Light sensing by plants, photomorphogenesis, phytochrome and blue light Photoreceptors, Effect of UV light on biological system, significance of biological clocks. Reproductive growth: Physiology and flowering, seed germination; dormancy and senescence.

10.

Book References:Calstone, A. W. and Davies. P. J. 1970, Control Mechanism in Plant Development. Prentice Hall inc. Englewood Cliffs. New Jersey.

Galstone, A. W. and Davies. P. J. 1980, A life of a Green Plant. Prentice Hall inc, Englewook Cliffs. New Jersey. Noggle. G. R. and Fritz, G. J. 1983, Introductory plant physiology, Prntice Hall Inc. Englewook Cliffs, New Jersey. Devlin. R. M. 1988, Plant Physiology-Reinhold pub. Co. New York. Galstone, A.W. and Davies, P.J. 1970, Control Mechanism in Plant Development. Prentice Hall Inc, Englewood Cliffs, New Jersey. Galstone, A.W. and Davies, P.J. 1980, A Life of a Green Plant, Prentice Hall Inc., Englewood Cliffs, New Jersey. Noggle, G.R. and Fritz, G.J. 1983, Introductory Plant Physiology. Prentice Hall Inc., Englewood Cliffs, New Jersey. Salisbury, F.B. and Ross, C.W. 1983, Plant Physiology, Wardsworth Pub. Co. USA. Delvin, R.M. 1988, Plant Physiology. Reinhold Pub. Co. New York.

GEB-206 Human and Animal Physiology Full Marks: Theory 40 + Tutorial 10 = 50 (1/2 Unit) 1.Time: 3 Hours

2. 3. 4. 5. 6.

7.

8. 9.

The circulatory system: Composition and function of blood and lymph, blood group and Rh factor, blood cell formation. Heart a brief introduction, anatomy of heart origin, conduction and regulation of heart beat, Cardiac cycle, Electrocardiogram. Blood pressure, Capillary pressure, Regulation of blood pressure. Disease related to the circulatory system. Respiration: Mechanism and control of breathing; transport of oxygen and carbon dioxide; Oxygen dissociation curve of hemoglobin and myoglobin; Bohr effect; Chloride shift: Human respiratory disorders. Digestion and Nutrition: Composition, function and regulation of salivary, gastric, pancreatic bile and intestinal juices, absorption, nutrition, balanced diet; importance of vitamins and minerals and trace elements; digestive disorders. Structure and function of Kidney: Micro architecture of Kidney, Composition of urine, physiology of urine formation, role of kidney in the regulation of water, salt and acid base balance, renal disorders. Muscle: Ultra structural chemical and physiological basis of skeletal muscle contraction, Molecular mechanism in muscle contraction. Nervous system: Structure of neuron, Nature of nerve impulse; origin and propagation along a neuron, membrance potential, action Synapse and myoneural junction. Integrative function of the central nervous system; Structure and function of sensory organs concerned with vision. Sound perception. Taste Smell and touch: receptors; Major neurophysiological disorders in humans. Endocrine system: Hormones and other signaling molecules; Hypothalamus. pituitary, thyroid, parathyroid, adrenal, pancreas and gonads; Other endocrine elements (pancreatic islets etc): Local chemical mediators prostaglandin; Consequence of endocrine malfunction. Principles of reproduction: The beginning, Reproductive modes, (asexual and sexual) and sexual: human reproduction and development. Liver: Basic structure of liver, Microstructure of liver, Physiological anatomy of liver, Functions of liver (Metabolic, General), Liver disease.

Book References:rArthur C. Guyton, M.D. & John E. Hall 2006, Text Book of Medical Physiology, W.B. Saunders Company, London. William F. Ganong, 2006, Review of Medical Physiology. 21st Edition. Dr. C.C. Chatterjee. Human Physiology, Vollume.1 & 2.

A.K. Datta. Human Anatomy, 7th Edition. B.D. Chaurasias. Human Anatomy ,Volume- 1, 2, and 3. Grays. Grays Anatomy. Mitter (only for picture). Shana and Ghosh. Human Physiology. Vision. A guide of physiology & biochemistry. Smith. E. Pateson. C.R. Scratecherd. T. and Read. N.W. 1988, Text Book of physiology. Hongkond. Elaine. N. Human Anatomy and physiology 4th Ed. Benjamin/Cummings. Scientific publishing, California. Thews.1982, Human Anatomy and physiology and Pathophysiology. Gyton, M.D. 1986, Text Book of Medical physiology. W.B. Saunders Company, London. Smith, E. Paterson, C.R. Scratecherd, T. and Read, N.W. 1988, Text Book of physiology. Elaine, N. Human Anatomy and Physiology, 4th Ed. Benjamin/Cummings Scientific Publishing, California. Thews, 1982, Human anatomy. Physiology and Pathophysiology.

GEB-207 Bioinformatics and Computer Application Full Marks: Theory 40 + Tutorial 10 = 50 (1/2 Unit) Time: 3 Hours

Bioinformatics1. History, Scope and Importance: Important contributions, aims and task of Bioinformatics, applications of Bioinformatics. 2. The Gen Bank Sequence Database: Introduction, Primary & Secondary databse, Format vs content: computer vs humans, GenBank Flat File dissection, GCG, ACDEB. 3. Information Retrieval from Biological Databases: Retrieving database entries, Integrated information retrieval: The entrez system, sequence databases beyond NCBI, TIGR, TAIR. 4. The NCBI Database: Introduction, SeqIDS, Bioseq: Sequences, Bioseqsets: Collections of sequences, Seq. Annot: Annotating the sequence, Seqdiscr: Describing the sequence. 5. Sequence Alignment and Database Searching: Introduction, Evolutionary basis of sequence alignment, Optimal alignment methods, Substitution scores & gap penalties, Statistical significance of alignments, Database similarity searching, FASTA, BLAST, Low complexity regions, Repetitive elements 6. Multiple Sequence Alignment: Progressive alignment methods, Motifs and patterns, Hocks, MOST, Probe, Presentation methods, Adscript 7. Phylogenetic Analysis: Elements of phylogenetic models, data analysis: Alignment, substitution model building, tree building and tree evaluation, building methods, searching for trees, hooting trees, Evaluating trees and data, phylogenetic software Some simple practical consideration. 8. Submitting DNA Sequences to the Databases: Introduction, Where to submit, What to submit, How to submit on the world wide web, How to submit with sequin.

Computer Application1. Applications: Multimedia systems, Computer networks, Network topology, Internet systems and Internet service, On-line and off-line, E-mail and www. 2. Selection of computer: Hardware, Software and cost consideration. 3. Package Programm: MS office, MS stat. 4. Maintenance: Power supply stability, grounding, effect of surge, sag current and its protection. Effect of static charge on computer devices. Handling of computer cards and chips. Computer viruses and protection. Software troubleshooting and maintenance Book References:S. Ignacimuthu, S.J. 2005, Basic Bioinformatics. Narosa Publishing House, New Delhi. A.D. Baxevanis and B.F.F. 2002, Bioinformatics, A practical guide to the analysis of genes and proteins Ouellette (Eds). John, Wiley and Sons. D.W. Mount, 2001, Bioinformatics. Sequence and Genome Analysis by Cold Spring Harbor Laboratory Press. S.C.Rastogi, N.Mendiratta and P.Rastogi, Bioinformatics-Prentice Hall.

T.K.Attwood and D.J.Parissmith Bioinformatics, Pearson Education. B.Bergeron. Bioinformatics. Computing. Pearson pub. David W Mount. Bioinformatics. CBS pub. Campbell & Heyer. 2003, Discovering Genomics, Proteomics, & Bioinformatics, Pearson Education, ISBN. 0-8053-47224. Baxevanis & Ouellette (2001) Bioinformatics, methods of Biochemical Analysis Series Vol. 43, John Wiley & Sons, ISNBN 0-471-38391-0. Pevzner, P.A. 2000, Computational Molecular Biology. MIT Press, ISBN: 0262161974 Andreas Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. D. Baxevanis & B.F. Francis Ouellette .2004, 3rd Edition. Wiley & Sons. ISBN. 0-471-47878-4. Peter Norton. Introduction to Computer. Balagurusamy E. Programming with ANSIC. Balagurusamy E. Programming in BASIC. Byron S.G. Avhaums outline of Programming with structured BASIC. An Annonimus. At certification complete manule.

GEB-208 Food and Nutrition BiochemistryFull Marks: Theory 40 + Tutorial 10 = 50 (1/2 Unit) Time: 3 Hours

1.

Vitamins: Structure, Co-enzyme activity, Deficiency symptoms, Dietary sources, requirements and bioassay of some vitamins. Minerals: Occurrence and role in metabolism, deficiency symptoms and requirements. Comparison of nutrient deficiency symptoms with diseases.

2. 3. 4.

Interrelationship between vitamins and micronutrients. Nutrient contents of food crops: Cereals, legumes, oil-seed, nuts, fruits, vegetables and their availability. Digestion: Digestion and absorption of protein, lipid and carbohydrates. Digestion and Nutrition: Composition, function and regulation of salivary, gastric, pancreatic bile and intestinal juices; absorption, nutrition, balanced diet; importance of vitamins and minerals and trace elements; digestive disorders. Food and Metabolism: Classification, source, chemistry and nutritional role of food stuff (protein, fat, carbohydrates) and their metabolism. Distinguishing features of plant metabolism. Energy metabolism: Basal metabolic rate, respiratory quotient and specific dynamic action of food. Balanced died: Diet chart for children, adult man and women, pregnant and lactating mother. Energy requirements according to age, sex and size. Nutritional problem: Nutritional problems in Bangladesh and to combat the malnutrition in Bangladesh. Dairy products: Nutrient content improvement.

5.

6. 7. 8. 9.

Book References:Albanese, A.A. 1980, Principles of Biochemistry. Worth Pub. Philadelphia. Boston, B.T. 1976, Human Nutrition Tara McGraw-Hill, New Delhi.

Conan E.E. and Stamp. P. K. 1984, Outlines of Biochemistry. John Wiley and Sons. New York. Clark. W. H. 1977, Experimental Biochemistry. Freeman and Company. Leningrad. A.1. 1980, Principles of Biochemistry. Worth Pub. Philadelphia. Marts and Porter. Laboratory Experiments in Biochemistry. Burgeons Pub. Co. Minneapolis. Street. 1976, Plant Metabolism. Perham Press. London. Deb, A.C. Fundamentals of Biochemistry. Swaminathan, M. Essentials of Food & Nutrition Volume-2 Mahtab S. Bamj, Rao P. and Reddy V. Textbook of human nutrition.

GEB-209 Plant Pathology Full Marks: Theory 40 + Tutorial 10 = 50 (1/2 Unit) 1. 2. 3. 4. 5. 6. Time: 3 Hours

7. 8. 9. 10 . 11 .

Brief history of plant pathology. Importance of plant pathology in modern agriculture. Definition and symptoms of plant diseases. Diagnosis of plant diseases and classification of plant diseases. Methods of studying plant diseases. Parasitism and disease development, stages in the development of disease. The disease cycle (i) Inoculation. (ii) Penetration (iii) Infection (iv) Dissemination of pathogens (v) Over wintering and over summering of pathogens. Defense mechanism of plants (i) Structural defense (ii) Biochemical defense Forecasting of plant diseases. Plant quarantine. Control of plant disease (i) Cultural methods (ii) Physical methods (iii) Chemical methods (iv) Biological methods. Study of the selected diseases of plants. Rice: Brown Spot, Blast, Bacterial leaf blight and Tungro disease. Wheat: Black rust, leaf rust and loose smut. Barley: Covered smart of barley. Sugarcane: Red rot, whip smut. Tea: Red rust, blister blight. Potato: Late blight and early blight. Jute: Stem rot and anthracnose.

Book References:G. Rangaswami. Diseases of Crop. Plants in India ,Practice Hall of India Pvt. D. K. JHA. A Textbook on Seed Pathology. Vikas Pub. House Pvt.Ltd R.S.Singh. Plant Disease. Oxford & IBH. K.A. Khaleque K.Z. Mamun. Practical Pathology and Microbiology. Aleya House. H.C. Dube. A Textbook of fungi, Bacteria and Viruses. Vikas Pub. House.Pvt.Ltd. R.S. Singh. Plant Pathogens. The Fungi. Oxford & IBH Pub. Co Pvt. S.B.Biswas, Amita Biswas. An Introduction to Viruses. Vikas publishing.

U. Sinha, Seela Srivastava. An Introduction Bacteria. Vikas Publishing House Pvt. R.S. Singh. Plant Diseases 7th ed. Vikas Publishing. R.S. Mehrotra. Plant Pathology. Tata Mc Graw-Hill. P.Gunasckaran. Laboratory Manual in Microbiology. New Age International P.L George N. Agrias. Plant Pathology, 4th ed. Elsevier India Pvt. Ltd.

THIRD YEARExamination: Part-III, Year: 2011

Course NoGEB-301 GEB -302 GEB -303 GEB -304 GEB -305 GEB -306 GEB -307 GEB -308 GEB -309 GEB-310 GEB -311 GEB -312 GEB -313 GEB -314

Course TitleTHEORY COURSES : Honours Courses Recombinant DNA Technology and Cell Signaling Plant Cell and Tissue Culture Biotechnology for Crop Protection Animal Biotechnology Molecular and Microbial Genetics Cytogenetics Biostatistics and Experimental Design Plant Breeding-II Animal Breeding Population Genetics PRACTICAL COURSES: Honours Covering Courses 301.302 & 303 Covering Courses 304.308 & 309 Covering Courses 305 & 306 Covering Courses 307 & 310 Class assessment: Tutorial / class attendance / Field report of Excursion (40+10+25) Viva-voce

Marks / Unit75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 75(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 50(Unit) 75(Unit) 50(Unit)1050 (10.5 Unit)

GEB-301 Recombinant DNA Technology and Cell Signaling Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. 2. 3. 4.

DNA/RNA probe for different molecular Biology techniques: Southern Blotting. Northern blotting, Western Blotting. DNA sequencing methods. Gene library: Construction of cDNA library and genomic library. Screening of gene libraries screening by DNA hybridization immunological assay and protein activity. Recombinant protein production in E. coli: Vectors for protein expression in E. coli. Over expression of recombinant proteins; Metabolic load. Fusion proteins. Purification of fusion protein. Recombinant protein production in yeast, Saccharomyces cerevisiae expression systems. S. cerevisiae vectors. Mammalian cell expression vectors: Selectable markers; Two-vector expression system two-gene expression vector. Bioreactor. Yeast twohybrid system. Gene targeting. Monoclonal and polyclonal antibodies.

5. 6. 7. 8. 9.

Book References:Glick. BR. and Pastenak. J. J. 1988, Molecular Biotechnology. ASM Press. USA. Giover. D.M and Hames. B.D. 1995, DNA Cloning 1 and 2 IRL press USA. Sambrook. J. Fritsch. E.F. Mariatis. 1999, Molecular Cloning. Aulaboratory. Manual. Cold. Watson. 1992, Recombinant DNA. Glick, B.R. and Pasternak, J.J. 2003, Molecular Biotechnology, ASM Press, USA. D.M. and Hames, B.D. 1995, DNA cloning 1 and 2 Glover, IRL. Press (Oxford University Press, USA) Sambrook, J., Fritsch, E.F., Mariatis 3 edition, 2001, Molecular Cloning, A laboratory Manual, Cold Spring Harbor Laboratory, U.S.A. Waston. 1992, Recombinant DNA. Alberts, Johnson, Lewis, Raff, Roberts and Water. Molecular Biology of the Cell, 4th edition. Lodish, Berk, Matsudaira, Kaiser, Krieger, Scott, Zipersky and Darnell.Molecular Cell Biology, 5th edition. Lehninger. Principles of Biochemistry. 4th edition, Nelson and Cox. Watson, Baker, Bell. Molecular Biology of the Gene. Glick, B.R. and Pasternak, J.J. 2003, Molecular Biotechnology. ASM Press, USA. Istavari Rasko and C. Stephen Downes, 1995, Genes in Medicine. Strachan, T.A. and Read A.P. 1996, Human molecular genetics.

GEB-302 Plant Cell and Tissue Culture Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. Time: 4 Hours

2. 3. 4.

5.

6. 7. 8. 9. 10 . 11 .

Introduction to tissue culture: History, importance, and scope, aims and objectives. Culture media: Laboratory organization. Sterile: Zation techniques. Nutrition of plant cells. Composition and preparation techniques for solid and liquid media. Plant growth regulators: Types and their uses in tissue culture. Tissue and organ culture: Establishment and maintenance of callus and cell suspension cultures, cellular differentiation and regulation of morphogenesis. Somatic embryogenesis: Principles and protocol; control of embryogenesis and organogenesis. Single cell methods. Cytology of callus; Tissue culture and Genetic Engineering. Tissue culture techniques: Haploid production, androgenesis; anther and microspore culture; Embryo rescue and culture; Micrografting in vitro pollination and fertilization; Protoplast isolation. Somatic hybrid and cybrids. Meristem culture: Isolation and culture of shoot tip and meristem confirmation techniques for pathogen elimination. In vitro selection of mutants: Mutant for salt, disease, cold, drought, herbicide and other stress condition. Artificial seed: Techniques for encapsulation, regeneration and preservation application and objectives. Single cell technique. Germplasm conservation: Cryopreservation and in vitro conservation. Secondary metabolite in cell culture. Tissue culture applications: Improvement of crop varieties through somaclonal and gametoclonal variation: sources and usages. Establishment of cell lines and evaluation: Application of tissue culture for crop improvement in agriculture, horticulture and forestry.

Book References:Kalyan Kumar De. 1995, An introduction to Plant Tissue Culture.. New Central Book Agency Bhowjwani S.S. 1990, Plant Tissue Culture to Application. an Limitations. Walton. 1988, Principles and Practices in Plant Science. P.D. Prentice Hall. Dixon. 1994, Plant Cell Culture: A Practical approach. Walton.1988, Principles and Practices in Plant Science. P.D.Prentice Hall. Bhowjwan.1990, Plant Tissue Culture: Application and Limitations, S.S. Dixon. 1994, Plant Cell Culture: A Practical approach. Anderson. L.A. 1992, Plant Cell Culture, Advance in Biochemical Engineering and Biotechnology Wasson, Recombinant DNA. Portykns, 1995, Gene transfer to Plants. Mantell and Smith, 1984.Plant Biotechnology. Losuge. 1983,Genetic Engineering of Plant . Kosuge. 1983,Biochemistry & Molecular Biology of Plant.

GEB-303 Biotechnology for Crop Protection Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

Biotechnology for crop disease management 1. Biopesticides: Development of antagonistic strains suitable for varying soil pH and moisture increase in efficiency of antibiotic production, chitinase gene, cloning of chitinase gene from microorganisms to another to increase biocontrol. Products efficiency bacteriocin, pseudobactin, development of mild strains for cross protection. Biological control: Biocontrol of plant pathogen, insects, pests and weeds, single cell protein. Toxin production by fungus, bacterial pathogens, induction of resistance in cells, defense gene, pathogenesis related proteins, chitinase gene, virulent gene, coat protein gene, satellite RNA gene MIC RNA gene, movement protein gene uses in genetic engineering. Stress tolerance: Drought, flood, salt and other tolerance genes. Biotechnology for pest management: 1. 2. Basis of entomology: Introduction to insect and pest, crop protection against insects. Role of biotechnology in pest management: Microbial control and biotechnology. Genetic improvement of natural enemies, mass production techniques- in vitro production of entomopathogens in cell lines, genetic engineering with Bacillus thuringiensis. Recombinant DNA technology and genetic control of insects: Transgenic plants. Bt toxin gene trypsin inhibitor gene, manipulation of biological rhythm in insects for their control, application of research in neuroendocrinology, biologically active peptides and botanical pesticides.

2. 3.

4.

3.

Book References:Bennet and Lasure. 1985, Gene Manipulations in Fungi. Graniti et al. 1989, Phytotoxins and Plant Pathogenesis. Hill. B.A 1988, methods in Plant Virology. Vidhyasekaran. P. 1988, Physiology of Disease Resistance in Plants. Vol. I & Vol. II. Burger. M.D 1981, Microbial Control of Pest and Plant Diseases. Academic Press London. Day. P. R.1986, Biotechnology and Crop Improvement and Protection. BCRC. Monograph. Kirsch Baum: J.B. 1985, Potential Implication of Genetic Engineering and Other Biotechnology to Insect Control. Ann. Rev. Entomol. Macia. S. 1989, Expression of Foreign Genes in Insects using Baculovirus Vectors Ann Rev. Entomol.

GEB-304 Animal Biotechnology Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1.

Introduction: Definitions, major techniques of animal biotechnology, application of animal biotechnology, aquaculture improvement through biotechnology, animal diseases. livestock production, recombinant DNA technology and animal bacteria improvement in nutritive value of low quality feeds. Principle of animal cell and tissue culture: Origin of the concept. Types of cells, primary culture, cell lines and cloning, somatic cell fusion, flask culture, organ culture and whole embryo culture. Techniques of cell and tissue culture. Application of Animal tissue culture. Animal tissue culture media: Blood plasma, blood serum, serum free media, tissue extracts, complex natural media, chemically defined media and other tissue culture media. Transgenic Animal Technology: Embryo transfer technology, MOET, In vitro fertilization: Transgenic mice, transgenic swine, transgenic cattle, collection of embryo, culture and transfer of embryos, methods for the introduction of recombinant DNA into chicken embryos, problems after developing transgenic animals. Study of some cell lines: HeLa cell, CHO cell. B-cell.

2.

3. 4.

5.

Book References:Bulock J. and Kristiansen B. Basic Biotechnology. Wisenan a. Principles of Biotechnology. John E. Smith. Biotechnology. R. C. Dubey. A Text book of Biotechnology. Lick and Pesternak. Molecular Biotechnology. S.S. Purohit. Agricultural Biotechnology.

GEB-305 Molecular and Microbial Genetics Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. Time: 4 Hours

Introduction: The evolution of microbial genetics; early concept of bacterial variation: Adaptation, mutation and selection, multinuclear conditions, dominance and recessiveness of characters.

2.

Bacteriological media, physical condition required for growth, normal growth cycle of bacteria. Quantitative measurement of growth, direct microscope count, the plate count method, membrane filter count, electronic enumeration of cell numbers, turbidometric method. Spectrophotometric method; isolation and identification of bacteria. Methods of maintenance and preservation of bacteria, periodic transfer, freeze-drying (lyophilization) and storage at low temperature. Coram sensing of bacteria. Genetics of Bacteria: Genetic transfer of characters; Bacterial conjugation, conjugation mating type. Recognition of factors. Chromosomal transfer, interrupted mating experiments chromosome mapping. Bacterial transformation- transformation of pneumococcal capsular types-cellular competence and environmental conditions required for transformationlinkages: Transduction-general, restored and abortive transductions; Fine structure study through transduction. Vectors: Plasmids as vector, pBR322 and other plasmid vectors, phage vectors, Cosmids, Phagemids; YAC and BAC vectors; Selectable markers in vectors. Genetics of fungi: Alternation of generation study of Aspergilllus nidulus. Neurospora crassa and yeast. The dynamic genome: Mobile genetic elements in eukaryotes (jumping genes)- relevance to plants; studies in maize; generation of antibody diversity. Genetics of Viruses: Genetics of bacteriophage (Lambda) and phage induced mutation. DNA and its gene organization. Site-specific recombination. Reverse genetics. T-DNA insertion mutants. Gene machines. Gene silencing. Epigenetic phenomena-transcriptional and post-transcriptional silencing. Epigenetic mechanisms in development.

3.

4.

5.

6. 7. 8. 9. 10 . 11 . 12 .

Book References:Bulock. 1987. Basic Biotechnology. Gibson. 2002.Microbial degradation of orginic compounds. Lizuka. 1981. Microbial conversion of steroid and alkaloids. Laskin. 1985. Enzymes and Immobilized cells in biotechnology, Smith J. E. 2009: Biotechnlogy, 5th ed, Cambridge University Press, New York. Glick B.R., Pasternak J.J. 2003: Molecular Biotechnology, 3rd ed, ASM Press, Washingoton D.C. Davis 1976 : Single Cell Protein. Laskin 1985: Enzymes and Immobilized Cells in Biotechnology. Gibson.1994: Microbial Degradation of Organic Compounds

Stanly R. Maloy, John E. Cronan Jr. David Freifelder.2006. Microbial Genetics 2 nd edn. Narosa publishing House New Delhi.

GEB-306 Cytogenetics Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. 2.

Introduction: Cytology and Cytogenetics. The chromosome complement: Morphological structure of prokaryotic chromosome, eukaryotic chromosomes, their nomenclature and their characteristics. Chemical organization of chromosome. Fine structure of chromosomes. Euchromatin, Heterochromatin, Karyotype and banding patterns of chromosome. Structural changes of chromosomes: Deletion, duplication, inversion, translocation: Definition, types, origin, meiotic behavior, role in evolution and uses in cytogenetical study. Numerical changes of chromosomes: Euploidy: Polyploidy in higher plants. Autopolyploidy: Origin and occurrence, Phenotypic effects. Meiotic behavior and meiotic configuration of autotriploids and autotetraploids, Breeding behaviour, Genetics of Autopolyploids. Allopolyploidy: Criteria for distinction of autopolyploids and allopolyploids, Allopolyploidization of autopolyploids; Genome analysis in allopolyploids and synthesis of new species using allopolyploidy. Aneuploidy: Source of primary, secondary and tertiary trisomics, source of monosomic and nellisomic locating genes through monosomic analysis. Meiotic and breeding behaviour of trisomic, monosomic and nullisomic. Characterization and identification of trisomics. Substitution lines: Cytogenetics of substitution lines. Production of different substitution lines and their genetic analysis.

3.

4.

5.

Book References:Gurdev S. Khush. Cytogenetics of Aneuploids. Academic press. New York & London. P.K. Gupta. Cytogenetics. Rastogi and company. Subasn Bazar, Meerut. India. P.S. Verma and V. K. Agarwal. Genetics S. Chand and company, Ramnagen. New Delhi. C.Y. Avers Genetics by Revised Edition. VR dnyanzagan. Cytogology and Genetics. Tota McGraw Publishing Company Ltd. New Delhi. Archana Sharma, Chromosomes (3rd ed.) Mohan primlani for oxford & IBH publishing Co. Pvt. Ltd. U. Sinha, Sunita Sinha. Cytogenetics, Plant Breeding and Evolution New Delhi, Vicks Pub. House Pvt. Ltd.

GEB-307 Biostatistics and Experimental Design Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. 2. 3. 4. 5. 6. 7. 8. 9. 10 . 11 . 12 .

Biostatistics and its scope. Source and presentation of data, pie chart, bar diagram frequency curve & histogram graph. Discrete and continuous variables, population sample and random samples. Measures of central tendency mean, mode and median measures of dispersion, range, variance, standard deviation, standard error, efficient of variation and confidence limits. Introduction to probability theory, probability rules, mutually exclusive and independent events. Distributions: binomial and Poisson distribution, normal curve and normal distribution and sampling from normal distribution. Hypothesis testing. Null hypothesis the 2 test of goodness of fit, and contingency 2 Comparison between two means, t-test and test of significance. The analysis of variance, one-way and two-way analysis and F-test. Multiple comparisons, least significant difference (LSD) and critical difference (CD). Measurement of association; covariance, correlation and test of its significance, regression analysis and regression line. Experimental Design: Principles and significance. Complete randomized design (CRD). Randomized complete block design (RCBD), Latin square design (LSD). Split-plot design (SPD) and Factorial Design.

Book References:Robert G.D. Steil & James H. Torre. Principles and Procedures of Statistics. A Biometrical approach, International student edition. W. Snedecor, William G. Cochran, Ames, Lowa. 1982, Statistical Methods Tth edu, George. U.S.A Kenneth Mather. 1972, Statistical Analysis in Biology by. Chapman & Hall, Science Paper back. Stanton a. Glaubj. Biostatistics 4th ed. The McGraw Hill Inc. Wayne W. Daniel. Biostatistics A foundation for analysis in the welth science John Wiley & Sons. Inc. W.G. Cochran & G. M. Cox. Experimental Designs. 2nd ed. A Wiley intn ed. K. A. Gomez. A. A. Gomez. 1976 Statistical Procedures for Agricultural Research with emphasis on rice. The Inter rice Reseech Inst. Munor R. Spiegel Statistics Schanms outline series McGraw-Hill Book company. Kwanchai A. Gomez. 1972, Techniques for field experiments with rice. The International rice research institute. Los Banos: Laguna. Philippines.

GEB-308 Plant Breeding-II Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. 2. 3. 4. 5. 6. 7. 8. 9. 10 . 11 . 12 . 13 . 14 .

Development of pure line variety and development of hybrid variety. Population improvement approach. Mutation and crop improvement. Haploidy & aneuploidy in crop improvement. Polyploidy and crop Improvement. Wide cross & crop Improvement. Tissue culture technique in crop Improvement. Somatic hybridization and crop improvement. Transgenic Breeding. Marker aided selection. Breeding for Drought & salinity tolerant. Breeding for Quality Traits: Protein & Oil content. Ideotype breeding. Release of new varieties: Notified crops and non-notified crops.

Book References:Allard, R.W. 1999, Principles of Plant Breeding. John Willey & Sons. New York. Chopra, V.L 1989, Plant Breeding. Oxford and IBH publishing Com. Ltd. New Dilhi. Dana, S. 2001, Plant Breeding Naya Udyog. Calcatta. Singh. B.D.1995 Plant Breeding-Principles and Methods. Kalyani Publishers. New. Sharma J.R 1994, Principles and practice of Plant Breeding . Chadhury R.C Introduction of Plant Breeding. New Delhi, Oxford & IBH pub-Co. Pvt. Ltd. Chawdhury H.K. Elementary Principles of Plant Breeding. New Delhi, Oxford & IBH Pub. Com. Pvt. Ltd. Rattan Lal Agarwal- Fundamentals of Plant Breeding and Hybrid Seed Production. New Delhi, Oxford & IBH Pub Co. Pvt. Ltd. U. Sinha, Sunita Sinha. Plant Breeding and Evolution. Cytogenetics, New Delhi, Vicks Pub. House Pvt. Ltd.

GEB-309 Animal Breeding Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. 2. 3. 4. 5. 6.

Introduction: Concept of Animal Breeding, its development and application, domestication of farm animals, development of breed association. Population parameters: Heretability and repeatability- definition, methods of estimation and their uses. Genetic and phenotypic correlation among the traits. Breeding value: Definition, measurement and uses, most probable producing ability. (MPPA) transmitting ability (TA). Selection: Natural and artificial selection, selection objectives and selection criteria. Selection method. Response to Selection: Prediction and estimation, implication to livestock improvement. Selection limit. Specialized breeding: Traits of economic importance, selection and breeding plants for the improvement of cattle, goat and poultry for specific purposes, systems of breeding for captive animals, conservation of endangered animal genetic resources. Mechanisms of Reproduction and Breeding: Male and Female Reproductive Organs. The sexual Cycle, Ovulation. Fertilization, implantation, Pregnancy Diagnosis in Animals; variation, choosing Traits for selection, Degrees of Relationship. Systems of Breeding. Heterosis of Hybrid vigor. Selection Methods, Fertility and Breeding Efficiency. Artificial insemination, Cryopreservation of semen and oocytes.

7.

8.

Book References:Warwick. E.J. and Legates. 1978, Breeding and Improvement of farm animal, 7 th ed. McGraw Hill Book Co. Inc. New York. Lasely. J. F. 1978, Genetics of Livestock Improvement, 3rd ed. Prentice Hall of India. New Delhi. Malcoim B. Willis. 1991, daltons Introduction to practical animal breeding , 3rd ed. Blackwell Sci. London. Mukherjee D.P, Banarjee G.C. Genetics and Breeding of Farm Amimals. New Delhi, Oxford & IBH Pub. Co. Pvt. Ralph Bogart. Scientific Farm Animal Production. Delhi, Surjeet Publication. Hafez Edi E. S. E. Reproduction in farm animals. Sunil Kumar Das. Poultry Production. New Delhi, CBS Publishers.

GEB-310 Population Genetics Full Marks: Theory 40 + Tutorial 10 = 50 (1/2 Unit) Time: 3 Hours

1. 2. 3. 4. 5. 6. 7. 8. 9.

Introduction: Historical Background and Development. Genetic Constitution of a Population: Frequencies of genes and genotypes, HardyWeinberg equilibrium, Multiple alleles, sex-linked genes. Changes of Gene Frequencies: As Selection; mutation, migration and drift. Mating and Reproductive system: Inbreeding, asexual of apomictic reproduction, assortative mating, measurement of inbreeding, out breeding and non-random mating. Speciation: Race formation, the species, Modes of speciation and isolation. Genetic polymorphism: A General account and the Basis. Continuous Variation: Metric charactar, General survey at the subject matter. Values and means: Component of means, breeding values, dominance deviation and interaction deviation. Variance: Components of variances, genetic components of variance, correlation and interaction between genotype and environment, environmental variance.

Book References:Hedrick. P. W. Genetics of population.1982, Jones & Bartlett Publisher, Inc. Bostone. M. W. Strickberger. Genetics. 2nd edition. Published prentic Hall of India Pvt.Ltd.M 97, New Delhi. Verma and Agarwal. 1989, Cell Biology. Genetics and Evolution. S. Chand & Company Ltd. Ramnayar. New Delhi.-110055. Verma and Agarwal. Genetics. D.S. Falconer. Introduction to Quantitative Genetics. Strickberger M.W. Evolution.1994. CBS Publishers & distributors 485. New Delhi.

FORTH YEARExamination: Part-IV, Year: 2012 Course NoGEB-401 GEB-402 GEB-403 GEB-404 GEB-405 GEB-406 GEB-407 GEB-408 GEB-409 GEB-410 GEB-411 GEB-412 GEB-413 GEB-414 GEB-415 GEB-416

Course TitleTHEORY COURSES:Biomedical Science and Genetic Engineering Industrial, Pharmaceutical and Environmental Biotechnology Immunology and Immunogenetics Agricultural Biotechnology Biotechnology of Microbial Systems DNA Fingerprinting and Molecular Diagnostics Enzyme and Enzyme Kinetics Cancer Biology Biometrical Genetics Biophysical Methods in life Sciences

Full Marks75 ( Unit) 75 ( Unit) 75 ( Unit) 75 ( Unit) 75 ( Unit) 50 ( Unit) 75 ( Unit) 50 ( Unit) 75 ( Unit) 50 ( Unit) 50 ( Unit) 50 ( Unit) 50 ( Unit) 50 ( Unit) 75 ( Unit) 50 ( Unit) 50 ( Unit)

PRACTICAL COURSES:Covering Courses 401 and 403 Covering Courses 402, 404 and 408 Covering Courses 406 and 408 Covering Courses 407.409 & 410 Class Assessment: Tutorial, Class attendance and Field report of Excursion (40+10+25)

Project Paper / Thesis / Dissertation Viva voce

Total: 1050 Marks (10.5 Unit)

GEB-401 Biomedical Science and Genetic Engineering Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

Human Genetics1. Structure of Human chromosome and DNA, Karyotype, chromosomal abnormalities in Human (Chromosomal aberration, chromosomal abnormalities and syndromes: structural

2. 3. 4. 5.

chromosomal abnormalities, numerical chromosomal abnormalities, Cri-du-chat syndrome, Down syndrome, Turner syndrome, fragile-X syndrome. Meta-females. Cystic fibrosis Klinefelter syndrome, XY males and XX females). Marfan disease. Metabolic disorders in human. Genetic causes for heart failure and other circulatory diseases. Neurogenetics and diseases of nervous system. (Huntington`s diseasse, Nurotibromatosis type I, Ataxiatelangiectasia. Sex-linked disease in human. Genetics of ageing. AIDS: Definition the biology of HIV, HIV and the immune system: Pathogenesis of AIDS: asymptomatic carrier-PGL-ARC-Full blown AIDS. Gene mapping and DNA fingerprinting. Cloning of human disease genes, Detection of mutations on human genes: Functional gene cloning; Candidate gene cloning and positional gene cloning. Human gene therapy: Ex vivo and in vivo gene therapy, Viral gene delivery systems, prodrug activation therapy, potential application of gene therapy in hemophilia. Nucleic acid therapeutic agents: Oligonucleotide correction of genetic conditions. Pharmacogenomics: Pharmacogenetics, pharmacogenetic technology, genetyring technologigs, Potential risk current and future Application of pharmaco genetics.

Genetic Engineering1. 2. 3. 4.

Book References:Recombinant DNA: Genes and Genomes. By- James D. Watson etc, Third Edition, Dec 8, 2006. Recombinant D N A. By- James D. Watson. First Edition, 1992. Foster G. D., Twell D. Plant gene isolation: principles and practice. Barbuik. L. A. Animal Biotechnology: Comprehensive Biotechnology. Phillips. J.P. and M. Moo-Young. H. C. Niyogi. A. K. and Srivastava. Human Genetics. Singer. Human Genetics. Mange. Human Genetics Basic. Mandal, I. S. Fundamental of Human Genetics. P. K. Dhar. Human Genetics. M. W. Donald. Immunology. Roitt. M. Immunology. K.J. Snustan. D.P. Simmons, Principles of Genetic, John Wiely & Sons, Inc. Lewn B. Genes VII, Oxford University Press U.K. Hart. 1998. Human Genetics. Lee. T.F. 1992. Human Genome Projects, Plenum Press, New York.

GEB-402 Industrial, Pharmaceutical and Environmental Biotechnology Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) 1. Time: 4 Hours

Biotechnology in industry and pharmaceuticals: Concept of Good manufacturing practice (GMP): Practice of GMP, quality control, quality assurance and inprocess control

2.

3. 4. 5.

6.

in pharmaceutical and industry. Production of pharmaceutical products: Monoclonal and polyclonal antibiotics, interferon, insulin, sometostain, human growth hormone antibodies, vaccine and immunosera. Test and techniques used for good pharmaceutical product: Sterility testing. Purification of pharmaceutical products. Environmental Biotechnology: Environment and sustainable development, biogeochemical transformations of carbon, nitrogen, sulfur and phosphorus. Environmental Pollution: Definition, nature of pollutions and types of pollution origin, effects and control systems. Waste Utilization: Biogas and biofertilizers: Development and optimization of membrane bioreactor process for use in sewage treatment. Application of biosensors for the detection of environmental pollutants. Isolation and enrichment of microorganisms capable of detoxifying environmental pollutants. Novel biotechnological approaches in waste treatment, genetic manipulation, enzyme and specialized bacteria. Microorganisms and metal pollutants. GMOs and Biosafety Regulations: Topics of concern related to the environmental release of genetically modified organisms (GMOs): (i) risk for animal and human health (ii) risk for agriculture (iii) risk of pollution with nontarget organism (iv) risk for the environment. Genetically modified foods: Benefits and risks, regulations, public acceptance and ethical issues. Bio-safety regulations to protect nature, growers and consumers interest and national interest.

Book References:Huge, W.B and Russel, Pharmaceutical Microbiology, A.D. Edited. Board, R. G. Allowodd, M.C. Bank, J. G. Edited: Preservatives in the pharmaceutical. Food & Environment Industries Blackwell Scientific Publication. Atlas and Bartha, Microbial Ecology. Klung and Reddy, Current Perspective in Microbial Ecology . Foin, 1, Ecological Systems, and the Environment. Wise D. L . Biotreatment systems, Volume 11. Mizrahi & Wezel, Advances in Biotechnological Process. Danforth & Wexel, Advances in Biotechnological Process. Danforth & Bakst, Biotechnology for sulving Agricultural Problems. Mitchell, R, Introduction to Environmental Microbiology. Handler P, 1970, Biology and the future of Man., Oxford University Press, U.K. Waston, J.D. and Tooze J. 1981, DNA Story W.H. Freeman and Company N.Y. Riclkef. R.E. 1990, Ecology, W.H. Freeman and Company N.Y. Raina & Maier et al., Elservier 2006: Environmental Microbiology. Agrawal, 2008, Industrial Microbiology. Vedpal. S. Malik 1992, Industrial Biotechnology, Tata Mc Grawhil Pub. Com. Ltd. D.P. Singh et al. 2005, Environmental Microbiology and Biotechnology.

GEB-403 Immunology and Immunogenetics Full Marks: Theory 60 + Tutorial 15 = 75 (3/4Unit) Time: 4 Hours

1.

Introduction to immunology and immune system.

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3. 4.

5. 6. 7. 8. 9.

Antigens, Antibodies and Immunoglobulin: Antigen-antibody reaction, Aglutination precipitation etc. Theories of antibody production, purification of antibody, Immunoglobulin, genes and diversity. Cells and tissues involved in immune response: T and B-cells development, maturation, activation, recognition and accessory molecules of T and B-cells. Cytokines: Main features of the best-studied cytokines, cytokine secretion and action, cytokine receptors and antogonists, the cytokine network and it`s role, immune-regulatory role of cytokine, role of cytokine in thymic development of T-cells. MHC: Discovery and function, class I and class II structure, antigen processing and presentation, genomic organization of MHC, MHC polymorphism. Hypersensitivity reaction, autoimmunity and tumor immunology. Immunization. Vaccines: Subunit vaccines-herpes simplex virus. Foot and mouth disease, tuberculosis, peptide vaccines. Genetic immunization, attenuated vaccines, vector vaccines. Genetic Basis of some disorders: Diabetes and it`s typing, genetic basis of Type-I DM. HLA-DQ. HLA-DR and MHC. Maturity onset diabetes mellitus in young (MODY): Phenylketonuria (PKU). Alkaptonuria, hemophilia, thalassamia, potential application of gene therapy in hemophilia. Immunological techniques : Vaccine production, ELISA for disease diagnosis, etc.

10 .

Book References:Snustad, D.P., Simmons, K.J. John Wiley Principles of Genetics, & Sons. Inc. Watson, 1992, Recombinant DNA. Lee. T.F., 1992, Human Genome Project, Plenum Press, New York. Human Genome News, Http:/www.ornl.gov/hgmis/publicat hgn/v 0nl/06snps. html. Bruce R., Korf, Human Genetics-A Problem Based Approach. 2000. Ivan Roitt Jonathan Brostoff and Devid Male, 1998, Immunology, 4th ed. Moshby, New York. Ian R. Tizard, 1996, Verterinary Immunology an introduction, 5th ed. W. B. Saunders Company. M. Wein. & John Stewart, 1997, Immunology. 8th ed, Danald. Churchill Lingstone. Longman Singapor publishers (Pvt) Ltd. Gillham, B. Despo, K. P. Thomas, J.H. Wills Biochemical Basis of Medicine. Jains Kuby, Immunology, W.H. Freeman & Co. NY. Nale, 1999, Immunology. Roitt, Brostoff. Roitt, 1998, Essential Immunology.

GEB-404 Agricultural Biotechnology Full Marks: Theory 60 + Tutorial 15 = 75 (3/4Unit) Time: 4 Hours

1.

Genetic Engineering of plants: Methodology of plant transformation with Ti-plasmid of Agrobacterium tumifacians, Ti-plasmid derived vector systems; Physical methods of transferring genes to plants- particle gun bombardment, electroporation. Use of reporter genes in transformed plant cells, Manipulation of gene expression in plants, Production of marker free transgenic plants, geno knockout. Application of plant genetic engineering: Development and application of agricultural hybrid and cybrid, somaclonal variation in crop improvement. Developing insectresistance, disease-resistance and herbicide resistance in plants for improving. Crop productivity and nutritional quality. Developing stress tolerance in plants; oxidative, salt and sub-mergence stress, fruit ripening. Genetic manipulation for storage of pro-vitamin A, iron proteins in rice. Modification of food plant taste and appearance, yield increase in plants, wild plant relatives as a source of novel genes. Biotechnology in seed production: Use of biotechnological methods for production and improvement of agricultural crop seeds. Merits and demerits of commercialization of genetically engineered seeds. Bio-pesticides: Bio-pesticide, bio-insecticide and weedicides, biological control, application of biotechnology for pest, insect and weed control, integrated pest management (IPM). Marker aided selection in plant breeding: RFLP, RAPD and AFLP, Marker assisted agricultural crop improvement. Genome mapping: Genome mapping efforts in rice and maize, Potential applications. Biotechnology and biodiversity: Definition, components, interaction, hierarchical pattern and scales of biodiversity, genetic diversity and implication, conservation of biodiversity in agriculture, application of biotechnology in biodiversity. Bio-safety and risk assessment: Bio-safety and risk assessment of genetically modified crops.

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6. 7. 8.

9.

Book References:Dubey R.C., A Text book of Biotechnology. Razdan M.K., An Introduction to Plant Tissue Culture. Sickeviz. Plant Biotechnology. Purohit. Agricultural Biotechnology. Trived P.R. and Sudarshan K.N., Environment and natural resources conservation. B.D. Singh, Biotechnology. Trived P. R. and Sudarshan K. N.Environment and natural resources conservation. B.D.Singh. Biotechnology, Expanding Horizons. Chhatwal G. R., Text book of Biotechnology. Kalyan Kumar De, An Introduction to Plant Tissue Culture. B.D.Singh, Plant Breeding- Principles and Methods.

GEB-405 Biotechnology of Microbial Systems Full Marks: Theory 60 + Tutorial 15 = 75 (3/4Unit) Time: 4 Hours

1.

2. 3.

4.

5.

6. 7. 8. 9. 10 .

Microbial production of therapeutic agents, Pharmaceuticals: Isolation of interferon cDNAs; engineering human interferon and human growth hormone; optimizing gene expression, enzymes-DNAase 1 and alginatelyase against cystic fibrosis. Monoclonal antibody as therapeutic agents- production of antibodies in E. coli. HIV therapeutic agents. Vaccines: Subunit vaccines-herpes simplex virus. Foot and mouth disease, tuberculosis, peptide vaccines. Genetic immunization, attenuated vaccines, vector vaccines. Synthesis of commercial products by recombinant microorganisms: Restriction endonucleases. Small biomolecules. Ascorbic acid. Indigo. Amino acid. Antibiotic. Biopolyers. Fermentation and biomass utilization: Microbial degradation of xenobiotics; Commercial production of fructose and alcohol; Silage fermentation; Utilization of cellulose. Economic importance of primary and secondary metabolites. Production of single cell protein from carbohydrates. N-alkenes, methane and methanol for use in food and feed. Liquid and gaseous fuel. Production of beer, wine, vinegar and distilled beverage; Microbial food products. Use of immobilized cell systems for the production of industrially important chemicals. Biomass utilization of food, fuels and chemicals. Role of microbes in dairy products, and flavours. Microbial leaching of heavy metals.

Book References:Primrose. Modern Biotechnology. WM Forgarty, CT Kelly, 1990, Microbial Enzymes and Biotechnology. JD Bulock, 1989, Basic Biotechnology. Gibson, 1994, Microbial degradation of organic compounds. H Lizuka, 1981, Microbial Conversion of steroid and alkaloids. AI Laskin, 1985, Enzymes and Immobilized Cells in biotechnology. Davis, 1976, Single. cell Protein. Smith J. E. 2009, Biotechnology, 5th ed, Cambridge University Press, New York. Glick B. R, Pasternak J. J., 2003, Molecular Biotechnlogy, 3rd ed, ASM Press, Washington D.C. Benjamin Lewin, Gene Series, 1-8, Ninth Edition. Tortora, 2009, Microbiology, Ninth Edition. P. Chakraborty, 2007, Text book of Microbiology. Michael J. Pelezar, 1997, Microbiology, Fifth Edition. Barnard R. et al , Molecular Biotechnology, Washington, ASN Press. D.P. Singh et al., 2005, Environmental Microbiology and Biotechnology.

GEB-406 DNA Fingerprinting and Molecular Diagnostics Full Marks: Theory 40 + Tutorial 10 = 50 (1/2 Unit) DNA Fingerprinting Time: 3 Hours

1. 2. 3. 4. 5.

Introduction and Basic genetic principle. Variable Number of Tandem Repeats Minisatellite sequences, Short Tandem Repeats (Stirs) Minisatellite sequences Hybridization based DNA fingerprinting (RFLPs): Radioactive method, Fluorescent method, Chemiluminsescent method. PCR-based DNA fingerprinting, Single locus and multi-locus DNA fingerprinting Polymorphism: Polymorphism of some genetic locus in relation to disease (HLA. APO and ACE gene) Applications of DNA fingerprinting: Criminal investigation (Personal identification), immigration, Paternity dispute, Identification of missing children, bodies found in plane crash road accident etc., Varietal identification of plants.

Molecular Diagnostics 1. Diagnosis of Cystic fibrosis by multiples PCR clinical implications abnormal mucus clearance from the respiratory tract with frequent infections, pancreatic insufficiency, abnormal salt transport, infertility in males. 2. Detection of Beta-Thalassemia mutation using ARMS PCR Clinical implications, Anemia (red cell deficiency) 3. Detection of Fragile X syndrome by FMR-i gene trinucleoide repeats analysis clinical implications. Mental retardation, ling faces, large ear, prominent jaws, post-pubertal macroorchidism. 4. Detection of philadelphia chromosome (BCL-ABL. (9:22) translocation by genomic southern hybridization. 5. Bone marrow engraftment: DNA analysis Am: To distinguish patent and donor cells as different using hyper variable tandem repeat polymorphic DNA markers. 6. Identification: of bacterial species based on the sequences of their 16S ribosomal RNA genes. 7. DNA Microarrays / DNA Chips / Gene Chips: Basic concept, Design of a DNA Microarry, Applications of DNA Microarry technology: Disease diagnosis, Drug discovery etc.

Book References:Micklos Davod A. and Freyer Greg A. 1990, DNA Science, Cold spring harbor Laboratory press and Carolina Biological Supply Company. Rob Reed, David Holmes, Jonathan. Weyers and Allan Jones. Practical Skills in Bimolecular Sciences. Addison Wesley Longman Ltd. 1998. Williams and Fleming. 1980, Spectroscopic Methods in Organic Chemistry. Walker, 1987, Techniques in Molecular Biology. Hamilton and Sewell, 1982, Introduction to HPLC. Ausubel, 1995, Short Protocols in Molecular Biology.

GEB-407 Enzyme and Enzyme Kinetics Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) Time: 4 Hours

1. Introduction: Brief history, Classification, nomenclature, enzyme assay, specific activity, enzyme activity units, enzymes as biological catalysts.

2. Factors affecting the rate of enzymatic reactions: Substrate concentration, enzyme concentration, pH, temperature, coenzyme and cofactor. 3. Enzyme kinetics: Mono-substrate reactions, Michaelis-Menten equation and its linear transformations. Km and Vmax: definition, determination, significance. 4. Enzyme inhibition: Reversible inhibition - competitive, non-competitive and uncompetitive inhibition; Irreversible inhibition. Michaelis-Menten equation. 5. Functional groups: Identification of functional groups essential for catalysis, ribonuclease and chymotrypsin as specific examples. 6. Factors affecting the efficiency of enzyme as catalysis: Proximity and orientation, covalent catalysis, acid base catalysis, metal ion catalysis. 7. Bisubstrate reactions: Single and double displacement reactions - random and ordered mechanisms. 8. Specificity of enzyme: Absolute specificity, broad specificity, intermediate specificity, and stereo specificity. 9. Active site of enzyme: Common features, enzyme substrate complex formation, and evidences. 10 Enzyme regulation: Allosteric enzymes, co-operativity special characteristics. Monod . and Koshland Models, covalent modification of enzymes, specific examples to be studied: ATCase, Glycogen phosphorylase, lactate dehydrogenase. 11 Mechanism of enzyme action: specific examples - chymo