Nineteenth International

Symposium on

Capillary Chromatographyand Electrophoresis

Wintergreen Conference Center

Wintergreen, Virginia, USA

May 18-22, 1997

UB/TIB Hannover 89

119 803 348

TABLE OF CONTENTS

LECTURES

M.J.E. Golay Award Lecture

M.J.E. Golay Award1

Fast Gas Chromatography: A Comparison of DifferentApproaches.Carel A. Cramers 2

Plenary Award Lecture

Applications of High-speed and Two-dimensional Gas Chromatography.

JohnB.Phillips 4

Microfabricated Separation SystemsFluidic Manipulations and Separations Using Microchips. J. Michael Ramsey 6

Integration ofBiochemical and Cellular Reactions with Separations On-chip.

P. JedHarrison. PerE. Andersson, Paul C.H. Li, Nghia Chiem,

Thompson Tang, R. Smith, R. Szarka, and T. Tran 8

Technologies for Nanochemistry and Nanoseparations. Johan Roeraade 10

Exhaustive Electromigration Injection from Small Volumes. Kazimierz Surowiec

and Purnendu K. Dasgupta 12

Electrophoresis in Flatland. Robert Austin. OlgicaBakajin, JeffChou,

Shirley Chan, and Thomas Duke14

Continuous On-lineSeparations with Electrophoresis in Narrow Channels:

From Small Neurochemicals to DNA Separations. Andrew G. Ewing.

Peter Gavin, Paula Beyer Hietpas, and Katherine Bullard 16

Microfabricated Integrated DNA Analysis Systems. Adam T. Woolley.

Peter C. Simpson, Stephen J. Williams, and Richard A. Mathies 18

Micro-capillary Electrophoresis Devices Fabricated Using Polymeric Substrates

and X-ray Lithography: A Logical Choice for High Resolution DNA

Separations. Steven A. Soper. SeanFord, Bill Kar, JackDavies, Mike Klopf,

Gina Calderon, andVolker Saile 20

MicrofabricatedDevices for Biological Analysis. Stephen C. Jacobson and

J. Michael Ramsey22

Enhanced Fluidity SeparationsHigh Pressure Gas Chromatography on Packed Capillary Columns.

Keith P. Bartle. Mark M. Robson, Peter Myers, and Anthony A, Clifford 24

Packed Column SFC: Advantages Over LC. Heinz Engelhardt 26

Packed Microcolumns for Novel Analytical Applications. Karin E. Markides.

Pernilla Koivisto, Susanne Wallenborg, Per Sjoberg, Benita Hyllbrant,Ulrika Petersson, F. Javier Seflorans, Bengt Langstrom, Leif Nyholm, and

Morgan Stefansson28

Advantages of Packed Capillary Columns in LC/SFC. Tyge Greibrokk.

Elsa Lundanes, Paal Molander, Iris Hunnes, andRoger Trones 30

I

New Applications of Enhanced-fluidity Chromatography. Susan V. Olesik.

Dong Wen, and Hao Yun 32

Solvating Gas Chromatography Using Packed Capillary Columns. Yufeng Shen

and Milton L. Lee 34

Analytical Extraction and Chromatography with Subcritical (Hot/Liquid) Water.

Steven B. Hawthorne. Yu Yang, Carol B. Grabanski, Arnaud Lagadec,

Kimberly J. Hageman, Maya Belghazi, and David J. Miller 36

Superheated Water as a Mobile Phase in Reversed Phase High Performance

Liquid Chromatography. Roger M. Smith. Robert J. Burgess, and

Orapin Chienthavorn 38

Addressing the Analytical Sample Preparation Needs in Industry.

Douglas E. Raynie 40

Capillary Liquid ChromatographyUltra-high Pressure Liquid Chromatography. John E. MacNair and

James W. Jorgenson 42

Gradient Elution Microchip Liquid Chromatography. Joerg P. Kutter.

Norio Matsubara, John W. Halliwell, Stephen C. Jacobson, and

J. Michael Ramsey 44

Evaporative Light Scattering Detection for Microcolumn LiquidChromatography. James N. Alexander IV 46

Multidimensional Microcolumn Liquid Chromatography for Complex Sample

Analysis. Johannes P.C. Vissers. Mario Ursem, Remco E.J. van Soest,

and Jean Pierre Chervet 48

Capillary HPLC/NMR Coupling 2D NMR Spectroscopy in the Nanoliter Scale

Structure Determination of Vitamin A Acetate Dimers. Gotz Schlotterbeck.

Li- Hong Tseng, Ulrich Braumann, and Klaus Albert 50

On-line NMR Detection for Capillary Separations. Jonathan V. Sweedler.

Dean L. Olson, Michael Lacey, and Andrew G. Webb 52

Advanced Sol-gel Column Technology for Condensed-phase Microseparations.Abdul Malik. James D. Hayes, Dongxin Wang, Sau L. Chong,

Gregory S. Corbett, and Jeff W. Cramer 54

Influence of the Length of the Nebulizer Drift Tube in a Laser Light-scatteringDetector and in the /t-LC-ICP-MS Coupling. Roger Trones. Anders Tangen,

Yngve Kristiansen, Walter Lund, and Tyge Greibrokk 56

Applications of Micro Packed Columns in Chiral Capillary Liquid Chromatography.Fernando M. Lancas. Andrea L. Rezemini, and Fernando A. Daher 58

Capillary ElectrochromatographyRecent Developments and Directions in Capillary Electrochromatography.

Csaba Horvath. Gargi Choudhary and Xian Huang 60

Sol-Gel Stationary Phases and Non-aqueous Mobile Phases for Electrochromatography:Will Any of This Work? JohnG. Dorsey. Ashley Lister, Paul B. Wright,Steven C. Wendelken, and Thomas L. Chester 62

II

Capillary Electrochromatography Versus High Temperature Capillary Liquid

Chromatography. Djordjevic M. Nebojsa, Fabrice Houdiere, Guido Lerch,andPatrickFowler 64

Capillary Electrochromatography in Open Tubes and Packed Columns.

Luis A. Colon. Yong Guo, Adam M. Fermier, Kimberly J. Reynolds,and Shirley Rodriguez 66

Practical Experiences and Applications of Capillary Electrochromatography (CEC)in the Pharmaceutical Industry. MelvinR, Euerby. Christopher M. Johnson,

Keith D. Bartle, Maria Cikalo, Peter Myers, and Stephanie C.P. Roulin 68

Varieties of Stationary Phases in Capillary Electrochromatography. Chuzo Fujimpto,Masahito Sakurai, and Yutaka Fujise 70

Electrochromatographyin Packed and Open Tubular Capillaries at Ambient

Pressure. Johan C. Kraak. S. Heemstra, R. Swart, andH. Poppe 72

Development and Evaluation of Capillary Electrochromatography for Analysis of

Pharmaceutical Samples. Kent M.Payne. Charles W. Demarest, Kurt R. Sedo,

Lai Y. Rwok, Thomas T, Catalano, Peter D.A. Angus, and John F. Stobaugh 74

Capillary Electrochromatography: Optimization of Instrumental Parameters.

Hans Rebscher, Andreas Banholczer, andUtePyell 76

CEC and CEC-ES-MS in Natural Product Research. Pat Sandra. Anna Dermaux,

andEric Van Hove 78

Novel Stationary Phases and Packing Materials for Capillary Electrokinetic

Chromatography. Vincent T. Remcho. Zhixin Jessica Tan, and

Michael Cipoletti 80

CEC with Predetermined Selectivity Created byMolecular Imprinting.StaffanNilsson. Leif Schweitz, and Lars I. Andersson 82

Capillary Electrochromatography (CEC) - Methodology and Applications.Monika M. Dittmann and Gerard P. Rozing 84

Capillary Electrochromatography: Advances in High-speed, High-efficiency and

High-selectivity Separations. Chao Yan. RajeevDadoo, Richard N. Zare,

Deon S. Anex, andDavidJ. Rakestraw 86

Capillary Electrochromatography: Evaluation of Some Column Fabrication

Techniques andApplication to PharmaceuticalAnalysis. Peter D.A. Angus,John F. Stobaugh. Charles W. Demarest, KentM. Payne, KurtR. Sedo,

Lai Y. Kwok, and Thomas Catalano 88

Chiral CapillaryElectrochromatography Using Capillaries Packed with

Chiralcel OD. Sally J. Grieb, Andrew Maxwell, and Joe P. Foley 90

Separation ofSynthetic Polymers by Electrically Driven Size Exclusion

Chromatography. Edward Venema. Johan C. Kraak, Hans Poppe,

and Rob Tijssen 92

Electrochromatography in Wide-bore Capillary Tubes. MarkM.Robson.

Peter Myers, andKeithD. Bartle 94

Liquid Phase Separations/Mass SpectrometryHigh Performance Capillary Electrophoresis/Mass Spectrometry: New

Developments and Applications. Steven A. Hofstadler. Richard D. Smith

and Joanne C. Severs 96

Generating Electrospray from Planar Glass Chips. Roswitha S. Ramsey and

J. Michael Ramsey 98

Micromachined Device for High Throughput Handling and Mass Spectrometric

Analysis of Biological Samples. Frantisek Foret, Qifeng Xue,

Yuri DunayevsMy, and Barry L. Karger 100

New Electroseparation Strategies Using Mass Spectrometric Detection.

Martin Mazereeuw and Jan Van der Greef 102

Recent Developments in SFC/Electrospray-MS for the Analysis of Pharmaceuticals

and Other Small Molecules. Timothy R. Baker. Thomas H. Eichhold,

J. David Pinkston, and Rosemary T. Hentschel 104

Microcolumn Separations Coupled with Mass Spectrometry for Optimizing

Protein-ligand and Enzyme-substrate Interactions. Daniel B. Kassel.

Ann Edison, Alison Meekhof, Kevin Blackburn, and W.Blaine Knight 106

Capillary Column HPLC Electrospray Mass Spectrometry of Combinatorial

Libraries. Kenneth C. Lewis. William L. Fitch, and Derek Maclean 108

Incorporation of a RAM Cell in a Microcolumn for On-column Detection of

Radiolabeled Molecules for LC-RAM-ESI-MS. Gary A. Schultz and

James N. Alexander IV 110

Capillary Electrophoresis/ElectrosprayIonization/Time-of-flight Mass

Spectrometry. Iulia M. Lazar and Milton L. Lee 112

Sequencing Biologically Derived Peptides at the Attomole Level: A Comparisonof the Utility of HPLC and CE Chromatographic Methods. Robert E. Settlage.Paul S. Russo, Jeffrey Shabanowitz, and Donald F. Hunt 114

High Resolution Capillary Isoelectric Focusing-Electrospray Ionization Mass

Spectrometry for Protein Characterization. Cheng S. Lee. Qing Tang,

Liyu Yang, andKamel A. Harrata 116

Basic Investigation and Application of MEKC-MS Using Atmospheric Pressure

Chemical Ionization. Koji Otsuka, Masaki Yokoi, Pirn Muijselaar, Hiroto Ozaki,

and Shigeru Terabe 118

Advances in the Understanding and Application of Capillary Electrophoresis

Inductively Coupled Plasma Mass Spectrometry. JohnW. Olesik.

Jeffrey A. Kinzer, and Eric J. Grunwald 120

Electrospray Nebulisation Interface for Micro-high Performance Liquid

Chromatography-inductively Coupled Plasma-mass Spectrometry of

OrganometallicCompounds. Mark W. Raynor. Maria Balcerzak,

Warren G. Pretorius, andLes Ebdon 122

Detection for Microcolumn Separations: The TOF-MS Advantage. Edgar D. Lee.

Alan L. Rockwood, Jacqueline P. Fabbi, Joseph C.H. Sin, Jeff Jones,

Chad Ogden, Hal Tolley, and Harold G. Lee 124

Gradient CapillaryHPLC in the Nanoliter Scale with Electrospray-MS and LD7/CCDDetection. Beate Behnke. Staffan Nilsson, Jonas Johansson, Christoph Kempter,J6rg W. Metzger, and Ernst Bayer 126

CE/Raman Spectroscopy: A Harmonious Marriage. Michael P. Morris.

Patrick A. Walker, III, Jeremey M. Shaver, and Hong- Min Li 128

Development of an Integrated Capillary Electrophoresis-Fiberoptic Sensor.

Michael J. Sepaniak. David L. Stokes, Jason E. Dickens, Victoria Tropina,and Tuan Vo-Dinh 130

Fast GC and GC-MS

Fast GC-MS with Supersonic Molecular Beams. Aviv Amirav and Shai Dagan 132

Realization of True Multi-channel Detection by High-speed GC/Time-of-flight MS.

John F. Holland and Ben D. Gardner 134

Environmental Air Monitoring with a Roving GC/MS System. William H. McClennen

and Henk Meuzelaar 136

Comprehensive Two-dimensional Gas Chromatography: Instrumentation Issues

Relevant to Portability. Edward B. Ledford. Jr.. Richard B. Gaines, and

John B. Phillips 138

Interfacing Fast Gas Chromatographic Techniques with Time-of-flight Mass

Spectrometry: Sharing the Burden of Component Resolution.

Ritchard C, Parry. Nancy Brichford, Viatcheslav B. Artaev, and Joel Mitchell 140

Analytical Advances in Biological Monitoring: Endocrin-disrupting and Other

Environmental Toxicants. Donald G. Patterson. Jr.. JohnR. Barr,

James Grainger, Vaughn Green, Chester Lapeza, Jr., Vincent Maggio,Sarath Sirimanne, C. Smith, Wayman Turner, and Adrian Woolfitt 142

Screening Environmental Samples for Oxygenates and Aromatics Using ComprehensiveTwo-dimensional Gas Chromatography. Richard B. Gaines. James D. Stuart,and Edward B. Ledford, Jr 144

Methods and Models for Selectivity Tuning in High-speed GC. Richard Sacks.

Heather Smith, and Mark Nowak 146

High-speed, Short Column Capillary Gas Chromatography with Chemometrics.

Robert E. Synovec. Carsten A. Bruckner, and Bryan J. Prazen 148

Fast Gas Chromatography Coupled with Mass Spectrometric Detection.

Jens Dalluge. Isabel Catalina, Ren6 Vreuls, Udo Brinkman, .and Eva Pocurull 150

New Approaches to Cryogenic and Related Solute Manipulation for Enhanced

Performance in Capillary Gas Chromatography. Philip J. Marriott and

Russell M. Kinghorn 152

Reducing Gas Chromatography Analysis Times with the New High Temperature

Multi-cap Column. William S. Cooke. Joseph W. Walsh, and Robert T. Wiedemer..

154

CE and MEKC

Capillary Electrophoresis of Charged Polysaccharides at High Resolution.

Milos V. Novotny. Jan Sudor, Mingfang Hong, and Morgan Stefansson 156

An "Immunosensor" Based on Capillary Electrophoresis. Robert T. Kennedyand Li Tao 158

On-line Coupling of Microanalysis Sampling to Capillary Electrophoresis.

Craig E. Lunte. Barry Hogan, Simon Y. Zhou, and Susan M. Lunte 160

On-line Injection in Capillary Electrophoresis. Christine E. Evans 162

Towards Classification of Pseudo-stationary Phases in MEKC. Morteza G. Khaledi.

MarkD. Trone, Michael S. Leonard, Jefferson G. Bumgarner, and

Armel A. Agbodjan 164

High Performance Capillary Electophoresis-Laser Induced Fluorescence of Glycoside

Toxins. Ziad EI Rassi and Arron Karcher 166

Dispersion in Micellar Electrokinetic Chromatography: Revisiting a Somewhat

Neglected Problem Child. Lixin Yu, Troy Seals, and Joe M. Davis 168

On-line ITP-CZE: Preconcentration and Clean-up Aspects. Dusan Kaniansky.Jozef Marak, Marian Masar, Jan Lastinec, Jetse C. Reijenga, and

Francis I. Onuska 170

Isotachophoresis: Trials, Tribulations and Trends in Environmental Analysis.Francis I. Onuska. Dusan Kaniansky, Milton L. Lee, and Kenneth D. Onuska 172

Single-Isomer Charged Cyclodextrins for Enantiomer Separations by CE.

J. Bryan Vincent, Hong Cai, Thanh V. Nguyen, Dawn M. Kirby,Alex D. Sokolowski, Billy A. Williams, and Gyula Vigh 174

Studies of Interconverting Systems by Dynamic Capillary Electrophoresis.Wilfried A. Konig. Detlev H. Hochmuth, Christian Wolf, and Gerald Weseloh 176

Use of a Dual Linear Photodiode Array for Enhanced UV Detection of Peptidesand Proteins in CZE and 2D micro-LC-CZE. Thomas F. Hooker and

James W. Jorgenson 178

Analytical Extraction/Concentration

On-line Membrane Extraction Microtrap Gas Chromatography (OLMEM-GC)for Continuous Extraction/Analysis of Organics from Air and Water.

Somenath Mitra. Xuemei Guo, and Naihong Zhu 180

Analytical Extraction, A Determinant of Throughput and Precision. Barry Kaye.David A. Stopher, and Richard F. Venn 182

Theory and Practice of Membrane Extraction with a Sorbent Interface.

Tadeusz Gorecki. Janusz Pawliszyn, M. Yang, Y. Luo, and M. Adams 184

Solvent Extraction into a Single Microliter Drop. Frederick F. Cantwell and

Michael Jeannot 186

New Developments in On-line Solid-phase Extraction-Gas Chromatography:

Newly Designed SPE-to-GC Transfer System and Sensor Controlled

Parameter Optimization. Rene" J.J. Vreuls. Thomas Hankemeier, and

Udo A.Th. Brinkman 188

Trace Level Analysis of Contaminants in Water and Air Using Sorptive Enrichment

in Packed Dimethyl-silicone Traps. Hans-Gerd Janssen. Erik Baltussen,Pat Sandra, and Carel A. Cramers 190

Large Volume Injection and On-line Transfer in Capillary GC. Konrad Grob 192

Use of On-column Large Volume Injection for the Analysis of Semi-volatiles in Water.

Mark Schneider, Marisa Bonilla. and Harold M. McNair 194

Automated Solid-phase Microextraction. Ralf Eisert and Janusz Pawliszyn 196

SPME/Micro-LC for Drug Analysis in Human Fluids. Kiyokatsu Jinno. Masahiro

Taniguchi, Makiko Hayashida, and Yokichi Ohno 198

State-of-the-art in Accelerated Solvent Extraction (ASE). Bruce E. Richter,John L. Ezzell, Richard E. Carlson, and Eric S. Francis 200

Supercritical Fluid Extraction of Wood Pulp with Analysis by Capillary Gas

Chromatography-Mass Spectrometry. Lori H. McDaniel.

Mehdi Ashraf-Khorassani, and Larry T. Taylor 202

VT

POSTERS

Gas Chromatography (Hydrocarbons and Fossil Fuels)1 Standardization and Automatization of Simulated Distillation Methods for

Routine Analysis. J .P. Durand. A. Bre, J.J. Beboulene, A. Ducrozet, and

S. Carbonneaux 204

2 Simulated Distillation of Petroleum Products by Capillary Gas Chromatography:Influence of Analytical Parameters. A. Bre. J.P. Durand, S. Carbonneaux,

D. Gabriel, J.J. Beboulene, and A. Ducrozet 206

3 Two Minute GC Simulated Distillation Analysis of Gasoline Range Material UsingShort 100-/tm Diameter Capillary Columns. Vince Giarrocco 208

4 Tar Bases from Resins of Brazilian Coal Tar. P.J. Sanchez Filho and E.B. Caramao 210

5 GC/MS (SIM Mode) Applied to the Quantification of Alkyl Phenols in Coal Tar.

I. Nascimento F°, M.G.R. Vale, and E.B. Caramao 212

6 Comparison of Solvents Used for Extraction of PAHs and Organic Matter from

Flue Gas. Marianna Czaplicka and Krystyna Kubica 214

7 Comparison of GC/FID and HPLC Methods for Determination of PAHs in Coal

Derived Materials. Krystyna Kubica, Marianna Czaplicka. Krystyna Tyrpien,and Teresa Kordas 216

8 High Resolution Capillary Gas Chromatography Using Three Different StationaryPhases to Identify and Quantify Polycyclic Aromatic Sulfur Heterocycles(PASH) in Oil Samples. Stephanie G. Moessner and Stephen A. Wise 218

9 Reliable PIONA Analysis of Complex Hydrocarbon Mixtures Using Capillary GC

and PID/FDD Response Ratios. Olga V. Napalkova, Paul P. Vulikh,

Igor A. Revelsky. Yuri S. Yashin, Igor N. Glazkov, Boris I. Zirko, and

Igor V. Galakhov 220

Gas Chromatography (Unique Applications)10 Analysis of Fire Debris for Water Soluble Accelerants. Q. Ren and Wolfgang Bertsch ....

222

11 On-line Reaction Gas Chromatography on the Nanoscale. Wolfgang Bertsch and

T. Moore 224

12 On-line Derivatization of Short Chain Fatty Acids in Headspace GC. Yuwen Wang,Maha Khaled, and Harold M. McNair 226

13 Identification and Quantitation of Thermal Decomposition Products of a Novolac

Resin by Pyrolysis-Gas Chromatography-Mass Spectrometry. C.A. Lytle,

Wolfgang Bertsch. S. Liu, S. Zhang, and M. McKinley 228

14 Organic Residues Analysis by CGC-MS in Floor Samples from the Hall of the

Eagle Warriors, Temple Mayor, Mexico. Araceli Pena. Luz Lazos,

Santiago Capella, Luis Barba, and Carmen Labastida 230

15 Chromatographic Behaviour of Underivatized Lidocaine and Metabolites in CGC.

Mohamed Abdel-Rehim. Margareta Bielenstein, and Torbjorn Arvidsson 232

16 Design and Optimization of a Benchtop GC Analyzer for Measuring Oxygenated

Compounds in Propylene and 1,3-Butadiene. Jim Luong. Lyndon Sieben,

and Jaap de Zeeuw 234

17 The Virtual Chromatogram®. Del E. Moore 236

VII

18 The Measurement of Dissolved and Total Water Content in Advanced Turbine

Engine Fuels Using a Gas-Liquid Chromatographic OTC Technique.

Wayne A. Rubey. Richard C. Striebich, and Steven D. Anderson 238

Gas Chromatography (Environmental)19 Development of Analytical Tools for the Determination of Airborne and Waterborne

Marine Engine Exhaust Contaminants. Todd Vanyo, Darning Li,Richard Morrison, and Vincent T. Remcho 240

20 Analysis of Organics in Complex Wastewaters. Laell Racovalis. Malcolm D. Hobday,Albert W. Hoveling, and Steven Hodges 242

21 Analysis of Sulfur Compounds by Solid Adsorbent Tenax GR Preconcentration

and Gas Chromatography with Flameless Sulfur Chemiluminescence

Detection. Wen-Fa Sye and Mei-Fang Cheng 244

22 Screening of Water Samples for PCBs at the ppt-ppq Level Using Direct LargeWater Sample GC/MS Analysis. Alexander I. Revelsky, Yuri S. Yashin,

Igor A. Revelsky. and Boris I. Zirko 246

23 FTIR Investigation of Organic Discharge in Polluted Water: IV. Monitoring of

Some Oil Fuel. Adrian Jeley. Claudia Barbu, Marian F. Ciobanu, L. Frunza 248

24 Limited Congener-number PCB Standard for Quantitation with Gas

Chromatography and Ion Trap Mass Spectrometry. Jack W. Cochran 250

25 Pesticide Analysis with the PFPD and a Novel Extract-free Direct SampleIntroduction Device. Aviv Amirav and Hongwu Jing 252

Gas Chromatography (Column Technology)26 Testing Column Performance. Roy J.M.N. Dresen, Huub J.W. Henderickx, and

Sjoerd van der Wal 254

27 Comparison of Capillary Column Selectivity for the Analysis of Essential Oils.

Leonard M. Sidisky. Kathleen H. Kiefer, and Greg A. Baney 256

28 Optimizing PCB Separations Using Gas Chromatography/Mass Spectrometry with

Unique, Low-bleed Stationary Phase Columns. Jack W. Cochran and

Shawn L. Reese 258

29 New In-situ Prepared Adsorbent for PLOT Columns with an Operational

Temperature Range from Subambient to 490°C. Jaap de Zeeuw and

J. Buyten 260

30 Effect of Various Binary Gas Mixtures as Mobile Phases on Theoretical Plate

Number in Capillary Gas Chromatography. Daniel L. Vassilaros.

Frank K. Schweighardt, Karen Yang, Michael T. Combs, and Larry T. Taylor 262

31 Analysis of Volatile Components in Alcoholic Beverages Using a Highly Selective Polar

Stationary Phase Which Is Water Resistent. Jaap de Zeeuw and N. Vonk 264

32 New Low-bleed 100% Styrene-divinylbenzene Porous Polymer with a Temperature

Stability up to 290°C. P. Heynsdijk and Jaap de Zeeuw 26633 Sol-gel Technology for Coating and Deactivation of GC Columns with High

Thermal Stability. Dongxin Wang and Abdul Malik 268

VTTT

34 Comparison ofArylene-containing Polysiloxane Polymers with Their CorrespondingPure Polysiloxane Capillary GC Stationary Phases. Dennis R.Gere and

Alan D. Broske 270

35 BPX70-Using ECL Numbers to Unravel Fatty Acid Methyl Esters. Mark Cumbers and

T. Norris 272

36 Unique Selectivity of Carborane Modified Phases for the Analysis ofPesticides.

Mark Cumbers and Russell Kinghorn 274

37 Applications on a New, Thermally Stable, High Cyanopropyl Capillary GC Column.Robert T. Wiedemer. Joseph W. Walsh, andDennis H. Mowrer 276

38 Use of a PoraPLOT Q-HT (TM) PLOT Column for the Analysis ofVolatile and

Active Organic Compounds. Jim Luong. Lyndon Sieben, Terry Dahoy,and Jaap de Zeeuw 278

39 Chromatographic Properties of Novel Inorganic Stationary Phases. Darin Sanchez,James LansbarMs, Beth McCulloch, and Wayne Rathbun 280

40 Cyclodextrin Doped Cyanosilicone Capillary Columns. Leonard M. Sidisky.AlokKumar, and Greg Baney 282

41 New Column for Amine Analysis in Capillary Gas Chromatography. N. Lammers,J. Buijten, Jaap de Zeeuw. and F. Biermans 284

42 New Silica-based Adsorbentfor Analyzing Volatile Compounds. C. Duvekot,

Jaap de Zeeuw. B. Schmidt, R. Schmidt, M. Mohnke, andR. deNijs 28643 New Porous Polymer Q Type PLOT Columns: Low Bleed, ExcellentImmobilization

and Inertness. Zhengb.ua Ji and Steve Hurt 288

Gas Chromatography (Detection)44 Gas Chromatographic Characterization of a Pear Aroma with Parallel Sniffing/Fn),

Sniffing/PID and MS Detection. Rizzolo Anna. Cocucci Maurizia,Tagliabue Silvia,andPolesello Stefano 290

45 Use of F-685 nm Atomic Emission Spectroscopy with GC for the Analysis of

Fluorine-containing Derivatives of Carbonyls and Carboxylic Acids in

Environmental Samples. Christof C.E. Simons. Paul J. Clarkson.

MalcolmR. Clench, andMichael Cooke 292

46 Influence ofthe Optical Configuration upon the Chromatographic Signals Generated

by a Microwave Induced Plasma Detector. Larisse Montero, Fabio Augusto.Ana Claudia Lemes, and Antonio Luiz Pires Valente 294

47 Trace Analysis of Sulphur Compounds in Natural Gas Using Large Volume Inj ection,Selective Enrichment and Sensitive SCD-Detection. Mark van Lieshout

Pham Tuan Hai, Hans-Gerd Janssen, and Carel A. Cramers 296

48 Screening for More Than 400 Pesticides by GC/AED: Reaping the Benefits of

Retention Time Locking. Philip L. Wylie, Bruce D. Quimby, and

DonaldD.Nixon 298

49 Enhanced Sensitivity and Selectivity of a Gas Chromatography-Microwave Induced

Helium Plasma Atomic Emission Detection System (GC-MIP) at the 685.6 nm

Fluorine Emission Line. Fabio Augusto and Antonio Luis Pires Valente 300

50 Fragmentation ofthe Trimethyl Silyl Derivatives of Substituted Aromatic Acids in

the Ion Trap Detector: Quantitation Study. Ibolya Molnar-Perl and

Kornelia Hovath 302

51 GC Dedicated Plasma Mass Spectrometric Detection. Cato Brede. Elsa Lundanes,

Tyge Greibrokk, and Stig Pedersen-Bjergaard 304

52 Design and Performance of a New ECD for the HP 6890 Series GC. Matthew S. Klee.

Imogene Chang, and Mahmoud Abdel-Rahman 306

53 Characterization of Products from Chemical Degradation and Pyrolysis of Humic

Organic Material Using GC/MS and GC/AED. Juergen Poerschmann and

Frank-Dieter Kopinke 308

54 Chemical Characterization and Quantitative Determination of DNA Damage, Induced

by Free Radicals, in Human Tumor Tissue by Gas Chromatography-MassSpectrometry. Gunther Guetens. Karel Van Cauwenberghe,Ernst Arthur de Bruijn, and Alex A.M. Eggermont 310

55 New Approach to the P- and N-containing Pesticides Reliable Identification -

Multiditection with NPD, PID and FID. Paul P. Vulikh, Elena V. Gitelmakher,

Igor A. Revelsky. Yuri S. Yashin, Boris I. Zirko, and Igor V. Galakhov 312

56 Gas Analysis by Gas Chromatography and Atomic Emission Detection.

J. Andreas Lippert. Paul B. Farnsworth, Milton L. Lee, Mingin Wu,Cole L. Woolley, and Edgar D. Lee 314

57 Determination of Organometallic Compounds in Hydrocarbon Matrices by HighResolution Gas Chromatography/Atomic Emission Detection. Jim Luong.Jonathan Mason, Terry Dahoy, Aaron Eidt, and Jaap de Zeeuw 316

58 Application of a Multicapillary Column to the Estimation of Electron Capture

Response: Is the ECD Concentration Dependent or Mass-flow Dependent?Masahiro Takeuchi, Kazuko Mizuishi, and Toshiyuki Hobo 318

59 Determination of Sameridine in Blood Plasma and Tissues by Gas Chromatographywith Nitrogen-sensitive Detection. Par Neidenstrorrt, Torbjorn Arvidsson, andCarina Norsten-H66g 320

60 Determination of Part Per Billion Levels of Light Chlorinated Hydrocarbons in

Complex Hydrocarbon Matrices by Gas Chromatography/ElectrolyticConductivity Detection. Jim Luong. D. Thibodeau, Aaron Eidt, and

Jaap de Zeeuw 322

Gas Chromatography (Natural Products)61 Investigating the Changes in Terpene Concentrations in Some of the Poplar Clones

Which are Most Often Used in Afforestation Practice Through the CGC-MSMethod. Krassimir P. Naydenov. Z.A. Rafii, and R. Dodd 324

62 Monoterpene Composition on in vitro Cultivated Plantlets of Aleppo Pine

(JPinus halepensis Mill). Marco Michelozzi, M. Lambardi, R. Tognetti,and C. Boggi 326

63 HRGC-MS Analysis of Terpenoids from Maytenus Aquifolium Martius and

Maytenus Ilicifolia Martius (Espinheira Santa). Fernando M. Lancas.

P.J.M. Cordeiro, and J.H.Y. Vilegas 32864 Simultaneous Analysis of the Main Constituents of Honeys by GC/MS as Their

TMS(Oxime)Ether/Ester Derivatives. Korn61ia Horv&th and

Ibolva Molnar-Perl 330

X

65 GC-MS Analysis of 21 Fragrance Substances in Deodorants. Suresh C. Rastogi.J.D. Johansen, P. Frosch, T. Menne, M. Bruze, J,P. Leppoitevin,K.E. Andersen, and I. White 332

66 GC Enantioseparation of Food Additives. Yuri P. Belov and Alok Kumar 334

67 Stereochemical Studies ofPlant Volatiles by Enantioselective Capillary Gas

Chromatography. Martin Pietsch. Nils Bulow, Christiane Fricke,Stefan Jung, Stephanie Melching, Angela Rieck, Yucel Saritas, and

Kolja Wihstutz 336

68 Differentiation of Twelve Pine Species from Central Portugal by Monoterpene

Composition Analysis Using HS-SPME and HRGC. Eduardo P. Mateus.

Q. H. Zhang, M.H. Farrall, andM. Rosa Paiva 338

69 Determination of Nonpolar/Neutral Composition of a Wood Pine Medium

Density Fiber Factory Effluent by High Resolution Gas Chromatography-

Mass Spectrometry and FT-BR. Spectrometry. Eduardo P. Mateus.

F. Santana, and I. Domingos 340

70 Occurrence of Trans Unsaturated Fatty Acids in Heteropappus hispidus and Some

Other Compositae Seed Oils. N. Tsevegsuren. K. Aitzetmuller, L. Bruhl, and

Gisela Werner 342

71 Capillary GLC Study of y-Linolenic Acidin Youngia tenuicaulis (Compositae) Seed

Oil. Nanzad Tsevegsuren. Kurt Aitzetmuller, and Klaus Vosmann 344

Gas Chromatography (Retention Measurement/Mobile Phase Manipulation)72 Effect ofAtmospheric Pressure on Capillary Column Gas Chromatographic

Retention Times. JohnV.Hinshaw. P.E. Schallis, andN.R. Green 346

73 Retention Time Locking: A New Tool for Method Development in Gas

Chromatography. Bruce D. Quimby and Matthew S. Klee 348

74 Influence of Carrier Gas Nature and Pressure on the Retention of Organic

Compounds in Capillary GLC. Victor G. Berezkin. Alexander A. Korolev,

and Irina V. Malyukova 350

75 Report on ASTM E-19 Task Force Group Re-examining Retention Indices and

Capillary Column Polarity Protocols. Dennis R. Gere. Alan D. Broske,

Linda Green, andRobert Trengove 352

76 Retention Time Locking in Capillary Chromatography: The Next Giant Step in

Practical Chromatography. Leonid Blumberg andMatthewS.Klee 354

Gas Chromatography (High Speed)77 Fast GC: 100 Jim R) Capillary Columns Maximizing the Use ofHigh Linear

Velocity and Fast Temperature Programming. Dermis R. Gere.

Alan D. Broske, and Linda Green 356

78 Advantages of Computer Assisted Data Acquisition for High Speed Packed

Capillary Chromatography. Christopher R. Bowerbank. Yufeng Shen,

andMiltonL. Lee 358

79 Strategies Toward Increasing Sample Throughput Using GC/Time-of-flight/MS.

BenD. Gardner and John F. Holland 360

80 Rapid Analysis of Air Particulates by Pyrolysis-Gas Chromatography/Ion Mobility

Spectrometry. WilliamH. McClennen. Sidney N. Thornton,

Jacek P. Dworzanski, HenkL.C. Meuzelaar, and Neil S. Arnold 362

81 Fast Analyses in the Food Sciences by GC/TOFMS. Ritchard Parry. Nancy Brichford,

Viatcheslav B. Artaev, Joel Mitchell, Randy Beaudry, and Jun Song 364

82 Parallel-column Gas Chromatography Coupled with Mass Spectrometry and

Chemometrics. Robert E. Synovec. Bryan J. Prazen, and Bruce R. Kowalski 366

Gas Chromatography (On-line Preconcentration Techniques)83 On-column Gas Chromatographic Sample Storage System for the Monitoring of

Multi-component Time-dependent Profiles. Jean-Marie P. Dimandja. and

JohnB. Phillips 368

84 Development of a Novel Microtrap System. Yoshiyuki Takei and Mitsuhiro Kurano 370

85 On-lineVOC Monitoring of the Raw Water Intake to a Maj or Water Treatment

Works by Purge and Trap-HRGC-MS. Helen Eggleston, Ivan Jepson, and

Ian A. Fowlis 372

86 Determination of Terpenes in Water Samples by Headspace Gas

Chromatography and Membrane Inlet Mass Spectrometry. Marja Ojala.Raimo Ketola, Timo Mansikka, Tapio Kotiaho, and Risto Kostiainen 374

87 On-site Monitoring System for Volatile Hazardous Air Pollutants Using CapillaryColumn GC/MS. Tsuneaki Maeda. Kouji Ichioka, Kaoru Funaki,Satoshi Arakawa, YukaYasuki, and Aki Sakai 376

88 Analysis of VOCs in Air Using a Portable Micro Gas Chromatograph in

Conjunctionwith Preconcentration Techniques. Hai Pham Tuan.

Hans-Gerd Janssen, Carel A. Cramers, Pbillippe Mussche, Jurgen Lips,Nigel Wilson, and Alan Handley 378

89 Two Stage Trapping for Continuous On-line GC Monitoring with a Microtrap.Chaohua Feng and Somenath Mitra 380

90 On-line Solid-Phase Extraction-Gas Chromatography-Ion Trap Detection (MS and

MS/MS) for Determination and Identification of Pollutants at the ng/1 Level.

Rene J.J. Vreuls. Arjan J,H. Louter, Krishna Verma, and Udo A.Th. Brinkman 382

91 Automated On-line Coupled SPE-Capillary GC: Applications to Ground Water

Pollution Control. Adriano Trisciani, Fausto Munari, Pier Albino Colombo, and

Sorin Trestianu 384

92 Large Sample Volume Capillary GC and GC/MS for Trace Analysis: Flexible,Modular Automated Multi-injector System Suitable for Cold On-column

and PTV Injection Techniques. Paolo Magni, Fausto Munari. and

Sorin Trestianu 386

93 In-vial Extraction and Large Volume Sample Injection: A Powerful Alternative to

Conventional Sample Preparation Procedures for Ground Water Pollution

Control. Paolo Magni, Fausto Munari. and Sorin Trestianu 388

94 Practical Approaches to Large Volume Injection. Philip L, Wylie and

DonaldD.Nixon 390

YTT

95 Comparison of Different Types of Drinking Waters Using Direct Large

Sample Volume GC Analysis, Maxim G. Lazutin. Michail A. Kucherenko,

Igor A. Revelsky. Yuri S. Yashin, Boris I. Zirko, and IgorN. Glazkov 392

96 Fully Automated Field Analysis ofAtmospheric Hydrocarbons Using Peltier

Cooled PTV-GC. Alastair C. Lewis andKeith D. Bartle 394

Supercritical Fluid Chromatography97 Supercritical Fluid Chromatography/Fourier Transform Infrared Spectroscopy

of Commercial Conjugated Linoleic Acid. Elizabeth M. Calvey.Martin P. Yurawecz, and Magdi M. Mossoba 396

98 Progress in the Development of an Open-tubular SFC/MS Method for the

Determination ofEthoxylated Alcohols in Environmental Samples.

J.DavidPinkston and Claudia A. Smith 398

99 Automated Systems Based on Supercritical Fluids. Andrei Medvedovici, Pat Sandra.

andFrankDavid 400

100 Chiral Separations by Supercritical Fluid Chromatography. Pat Sandra.

Andrei Medvedovici, Laura Toribio, and Frank David 402

101 Advantages of Small Particles in SFC. Christopli Siffrin and Heinz Engelhardt 404

102 HPLC with Subcritical (Hot/Liquid) Water and FID Detection. David J. Miller

and StevenB. Hawthorne 406

103 Performance of Packed Capillary Column Chromatography Under Conditions

of Large Pressure Gradients and Phase Transformations. Yufeng Shen

andMiltonL. Lee 408

104 Comparison of Packed Capillary Solvating Gas Chromatography with SupercriticalFluid Chromatography. Naijun Wu. Yufeng Shen, and Milton L. Lee 410

105 Oxidative and Reductive Amperometric Detection of Phenols and Nitroaromatic

Compounds in Packed CapiUary Column SFC. Susanne R. Wallenborg.

KarinE. Markides, andLeifNyholm 412

106 Reversed-phase Liquid Chromatography with Flame Ionization Detection Using

Water as the Eluent. Robert J. Burgess and Roger M. Smith 414

107 Applicabilities ofLong Packed Capillary Columns in Chromatography.Iris Hunnes, Paal Molander, Cathrine Thomsen, Lai M.T. Nguyen,

Inge Bruheim, Lise Marken, Line Roed, Cato Brede, Roger Trones,

ElsaLundanes, and Tyge Greibrokk 416

Capillary Liquid Chromatography108 Optimization of the Use of Cyclodextrins in the Enantiomeric Separation of

Terpenes in Citrus by (i-LC and CE. Fernando M. Lancas. C.A. Diagone,

and C.A. Ogawa 418

109 Modification of Polytetrafluoroethylene (Teflon®) Particles for Chromatographic

Applications. Luis A. Colon. Rafael Alicea-Maldonado, and Joseph A. Gardella, Jr 420

110 Different Gradient Modes in Micro and Capillary HPLC. Fabrice Houdiere,

Patrick Fowler, and Djordjevic M. Nebojsa 422

111 Advantages of MicroLC Coupled to Mass Spectrometry. Pernilla Koivisto.

Benita Hyllbrant, Lena Nyholm, Per Sjoberg, and Karin E. Markides 424

112 Pump Technology for Ultra High Pressure Capillary Liquid Chromatography.

John E. MacNair and James W. Jorgenson 426

113 Fused Silica Capillary Columns Protected by a Flexible Housing. Bijan Modrek 428

114 Photo-alternative Stationary Phases for Semi-micro HPLC and Capillary LC.

Hideo Matsuoka, Tatsuro Nakaeama. and Toshiyuki Hobo 430

115 Pressure-induced Perturbations in Solute Complexation in Capillary Separations.Christine E. Evans and Moria C. Ringo 432

116 Microdialysis/SFE/SFC/FJD of Antioxidants and Related Compounds in Water.

F. Javier Setiorans. Ulrika Petersson, and Karin E. Markides 434

117 Comparison of Reversed-phase HPLC Separation Using Carbon Dioxide and

Fluoroform for Enhanced-fluidity Liquid Mobile Phases.

Huimin Yuan and Susan V. Olesik 436

118 Reversed-phase Microcolumn LC as an Effective Preconcentration Techniquefor MALDI/MS and NMR Studies ofDerivatized Oligosaccharides.P. ChenandM.V.Novotny 438

119 A Compact, Portable Capillary Ion Chromatograph: C. Brad Boring and

Purnendu K. Dasgupta 440

120 High Throughput, High Resolution Microcolumn LC/MS Separations for

Assessing Purity and Biological Activity of Combinatorial Libraries.

Daniel B. Kassel. Lu Zeng, Lyle Burton, and Kai Yung 442

121 Performance Testing of an Eluent-Jet Interface for the Coupling ofMicroflow

Liquid Chromatography with Electron Impact and Chemical Ionization

Mass Spectrometry. Rene J.J. Vreuls. Reijer J. Dijkstra, Ben L.M. van Baar,Charles E. Kientz, and Udo A.Th. Brinkman 444

122 Microelectrochemical Detector for Use at Low Linear Velocities in CapillaryColumn Systems. Susanne R. Wallenborg. Karin E. Markides, and

LeifNyholm 446

123 Controlling Extra-column Dispersion in Microbore and Capillary HPLC.

RichardLudwig andRichardHenry 448

124 Characteristics of High Temperature Micro Liquid Chromatography.Paal Molander. Roger Trones, and Tyge Greibrokk 450

Capillary Electrochromatography125 Analysis of Environmental Toxicants in Human Serum by Cloud-point Extraction

and Capillary Electrochromatography. Sarath R. Sirimarme. Li Ma, John Barr,and Donald G. Patterson, Jr 452

126 Enantiomeric Resolution of Dansyl Amino Acids by Capillary ElectrochromatographyUsing Molecularly Imprinted Polymers as Recognition Agents. Jin-Ming Lin,

Yunhua Gao, Tatsuro Nakagama, Katsumi Uchiyama, and Toshiyuki Hobo 454

127 Use of Step-gradient Capillary Electrochromatography (CEC) with CommerciallyAvailable Capillary Electrophoresis (CE) Instrumentation. Melvin R. Euerby.Declan Gilligan, Christopher M. Johnson, and Keith D. Bartle 456

128 Rapid Analysis Capillary Electrochromatography (CEC) Using the "Short-end

Injection" Capillary Electrophoresis (CE) Approach. Melvin R. Euerby.Christopher M. Johnson, Keith D. Bartle, Maria Cikalo, and Peter Myers 458

129 Capillary Electrochromatographic Performance of a New Mixed Mode (C18/SCX)

Stationary Phase. Melvin R. Euerby. Christopher M. Johnson, Helen Lomax,and Paul Ross 460

130 Evaluation ofthe Fundamental Properties of Capillaries Coated with Different

Ion-exchangers. Maria G. Cikalo. Keith D. Bartle, and Peter Myers 462

131 New Developments in Capillary Gel Electrochromatography for the Analysis of

Small Molecules. Chuzo Fujimoto. Masahito Sakurai, and Yutaka Fujise 464

132 Molecular Imprinted Stationary Phases for Capillary Electrokinetic

Chromatography. Zhixin Jessica Tan, Michael Cipoletti, andVincent T. Remcho 466

133 Transfer ofHPLC Methods and Applications to Capillary Electrochromatography

(CEC). Gordon Ross, Monika M. Dittmann. and GerardP. Rozing 468

134 Characterization of Stationary Phases for CEC. Frank T. Hafher. M. Scherer, and

H. Engelhardt 470

135 Design and Evaluation of a NewCEC System. Baomin Xin. Samuel E. Tolley,andMiltonL.Lee 472

136 Macroporous Polyacrylamide/Polyethylene Glycol Matrices as Stationary Phases

for Capillary Electrochromatography. Anders Palm andMilos V. Novotny 474

Capillary Electrophoresis (Detection)137 Photodiode Array for Multi-point (Spatial) UV Absorption Detection in Capillary

Electrophoresis. Karen A. Burton, Thomas F. Hooker, ChristopherT. Culbertson, and James W. Jorgenson 476

138 Analysis of Chlorinated Phenoxyalkyl Acid Herbicides in Water Using Capillary

Electrophoresis/Negative Electrospray/Mass Spectrometry. Xinbei Song and

William L. Budde 478

139 Acquisition System Requirements for Micro Separations Time-of-flight Mass

Spectrometry. AlanL. Rockwood, Edgar P. Lee. Jacqueline P. Fabbi,

Joseph C.H. Sin, Larry Davis, Metin Gunsay, Logan Harris, Chad Ogden,Hal Tolley, and Harold G. Lee 480

140 CE-TOFMS for the Analysis of Paraquat and DiquatHerbicides. IuliaM.Lazar

andMiltonL. Lee 482

Capillary Electrophoresis (Biomacromolecules)141 Diastereomeric and Enantiomeric Separation ofPeptides Containing Aspartic

or Glutamic Acid by Capillary Electrophoresis: Application to StabilityStudies. Gerhard K.E. Scriba. Sigrid Zimmermann, and Samir Sabah 484

142 High-speed High-resolution Separation of DNA Fragments by Capillary

Electrophoresis in Cellulose Derivative Polymer Solutions. LineRoed,

Elsa Lundanes, and Tyge Greibrokk 486

143 DNA Analysis by Electrophoresis Microchip Device. Larry C. Waters, JuliaKhandurina.

N. Kroutchinina, R.S. Foote, Stephen C. Jacobson, and J. Michael Ramsey 488

144 Capillary Electrophoresis of Plasmids and Linear Nucleic Acids in Dilute PolymerSolutions. Richard W. Hammond, Jeffrey J. Schwinefus, Shau-Chun Wang, and

Michael D. Morris 490

145 High Resolution Separation of DNA Restriction Fragments Using Capillary

Electrophoresis with Near-infrared Laser-induced Fluorescence Detection.

S.Kar. S.M. Ford, C.V. Owens, Y.Y. Davidson, and S.A. Soper 492146 Sensitive Analysis of [D-PEN^JEnkephalin in Rat Serum with Capillary

Electrophoresis and Laser-induced Fluorescence Detection. Cuiping Chen.

Dorothea Jeffery, James W. Jorgenson, M. Arthur Moseley, and

GaryM. Pollack 494

147 DNA Base-pair Mismatch Detection Using Mismatch Repair Enzyme Cleavageand Capillary Electrophoresis. Barbara A. Siles. David L. Tung,G. Bruce Collier, Christopher LP. Lovelace, and Martin L. Smith 496

148 In-line Coupling of the Enzymatic Degradation of Oligonucleotides with Capillaryolymer Sieving Electrophoresis. Jan Saevels, Ann Van Schepdael,

Eugene Roets, and Jos Hoogmartens 498

149 Electrochromatographic Contributions in Capillary Electrophoresis of Biomolecules.Xiaowen Shao. Yufeng Shen, Kim O'Neill, and Milton L. Lee 500

150 Analysis ofthe Protein Matrix ofHubridoma Cell Culture by SDS-GEL CapillaryElectrophoresis. VadimE. Klyushnichenko, Holger Heine, Manfred Biselli, and

Maria-Regina Kula 502

151 Separation ofHyaluronate Oligomers through End-label Free Solution Electrophoresis.Mingfang Hong and Milos V. Novotny 504

Capillary Electrophoresis (Unique Applications)152 Separation and Analysis ofBacterial Cells and Cell Constituents by Capillary

Electrophoresis. Roy MA. Lautamo and David T. Mao 506

153 A Method of Analysis ofRecombinant Acidic Fibroblast Growth Factor (aFGF)by Capillary Electrophoresis. Thomas P. Roddy. Thomas E. Molnar,Robert E. McKean, and Joe P. Foley 508

154 Capillary Electrophoresis Applied to PhysicochemicalMeasurements in Pharmaceutical

Analysis. Jinping Liu. Charlene La, Kevin J. Volk, Sadia Abid, andMike S. Lee 510

155 Ultra High Voltage Capillary Zone Electrophoresis. Katariina M. Hutterer and

James W. Jorgenson 512156 Capillary Electrophoretic Techniques for Drug Analysis in Human Fluids.

Kiyokatsu Jinno. Yinghong Han, Hirokazu Sawada, Makiko Hayashida,and Yokichi Ohno 514

157 Capillary Electrophoretic Performance of Sol-gel Columns with SupercriticalDrying. James D. Hayes and Abdul Malik 516

158 Nonaqueous Capillary Electrophoresis. Morteza G. Khaledi. Joseph L. Miller,Fang Wang, Wei Zhou, and Vicki B. Ward 518

159 pH-Mediated Stacking of High Ionic Strength Samples for Capillary Electrophoresis.Sangyroul Park, Yeping Zhao, and Craig E. Lunte 520

160 Capillary Electrophoresis of Chiral Pyrethroid Insecticides. Ziad El Rassj and

Arron Karcher 522

161 Determination of Melphalan and its Hydrolysis Products in Perfusion Samplesof Cancer Patients by CE. Gert de Boeck, Karel van Cauwenberghe,Alexander A.M. Eggermont, Ernst A. de Bruijn, and Allan T. van Oosterom 524

162 Determination of Nitrophenols by Isotachophoresis and Capillary Zone

Electrophoresis. Francis I. Onuska. Dusan Kaniansky, and Jozef Marak 526

163 Determination of a Glutathione Adduct of Atrazine by SPE and CapillaryElectrophoresis. Massimo Rossi, Luana Dragani, Vinicio Sperandio,Rita Frassanito, and Domenico Rotilio 528

164 Dynamic Channel Electrophoresis. Jonathan V. Sweedler and Yi-Ming Liu 530

165 Migration Markers for Capillary Isotachophoretic Separation of Ribonucleotides.

Shujun Chen and Milton L. Lee 532

166 Determination of Morphine in Human Hair Using Isotachophoresis and CapillaryZone Electrophoresis. Kenneth D. Onuska and Milton L. Lee 534

167 Polypropylene Hollow Fibers Modified by PMMA for Capillary Electrophoresis.Shize Qi and Milton L. Lee 536

168 Plastic Microfluidic Devices Prepared by Imprinting Techniques. Laurie E. Locascio.

Larisa Martynova, Michael Gaitan, William A. MacCrehan, Gary W. Kramer,and Richard G. Christensen 538

169 Discrimination of Foam Concretes from Gas Concretes by Capillary Electrophoresis.Heili Siren and Jari Pukkila 540

Capillary Electrophoresis and Chromatography (Fundamental/Modeling)170 Calculation of Predicted Migration Rates in Capillary Electrophoresis from

Molecular Structure. Roger M. Smith. Andrew G. McKillop, Ray C. Rowe,

and Stephen A.C. Wren 542

171 Estimation of the Variance in Electrophoretic Mobility by Measuring the Peak

Width and Asymmetry on Time Domain Electropherograms.Brian S. Weekley. Joe P. Foley, and Greg Mclntire 544

172 Computer Simulation of Capillary Zone Electrophoresis and Micellar Electrokinetic

Chromatography. Arthur B. Coddington and Joe P. Foley 546

173 Mathematical Modeling and Optimization of Electroinjection Analysis and

Electrophoretically Mediated Microanalysis. Victor P. Andreev.

E.V. Lebedeva, and N.S. Pliss 548

174 Explanation for Success of New Statistical Models of Overlap with Poorly Resolved

Separations. Joe M. Davis 550

175 Modeling Concealment in Microcolumn Separations. H. Dennis Tolley,Steven J. Butala. and Milton L. Lee 552

Micellar Electrokinetic Capillary Chromatography and Chiral CE

176 Micellar Electrokinetic Chromatography (MEKC) of S-Triazine Herbicides.

Fernando M. Lancas and M.A. Ruggiero 554

177 Evaluation of Methacrylate Copolymers as Pseudo-stationary Phases for

Electrokinetic Chromatography. Christopher P. Palmer 556

178 Influence of the Inorganic Counterion on the Chiral Micellar Electrokinetic

Separation of Basic Drugs Using the Surfactant N-Dodecoxycarbonyvaline.Alicia G. Peterson and Joe P. Foley 558

179 Comparison of the Retention Behavior of Beta-blockers and Octanol-water

Partition Coefficient Estimation Using Immobilized Artificial Membrane

Chromatography and Lysophospholipid Micellar Eletrokinetic

Chromatography. John A. Masucci. Gary W. Caldwell, and Joe P. Foley 560180 Micellar Capillary Electrophoresis (MCE) Use for Determination of Biocides

in Paper Food Packaging Materials. Shirley Abrantes, Mark Philo,Andrew P. Damant. and Laurence Castle 562

181 Chiral CE with Crown Ethers. Katleen Verleysen and Pat Sandra 564182 On-line Micellar Electrokinetic Chromatography-Electrospray Ionization Mass

Spectrometry for Protein Characterization. Cheng S. Lee and Liyu Yang 566183 Urate and Ascorbate Levels in Premature Infants' Lung Lavage. Qirtglin Tang. Hai

Wang, Thomas T.-S. Huang, William L. Stone, Des Bharti, and Milton L. Lee....

568184 Evaluation of Micelle Modifiers for use in Micellar Electrokinetic Capillary

Chromatography. Kent P. Demon. William E. Wall and Joel T. Smith 570185 Enantiomeric Separations of Isoflavonoids from Legumes Using Capillary

Electrophoresis. Darin J. Allen. Joel T. Smith, William E. Wall, Kent D. Densonand Nancy Paiva 572

186 Investigation of Electrokinetic Chromatography Using Thermodynamically-stableVesicles and Mixed Micelles Formed from Oppositely Charged Surfactants.Mei Hong and Joe P. Foley 574

Micromachining in Microcolumn Separations187 Microfabricated Devices for Enzyme Assays. Andrew G. Hadd. Stephen C. Jacobson and

J. Michael Ramsey 576188 Confocal Single Molecule Detection on Microchips. Julius C. Fister III, Stephen C.

Jacobson, and J, Michael Ramsey 578189 Microfabricated, Recycling Electrochemical Detector for Capillary Separations.

Sunday A. Brooks and Robert T. Kennedy 580

Multidimensional

190 Comprehensive LC/LC/MS of E. coli Lysates to Separate, Identify, and Characterize

a Protein of Pharmaceutical Interest for Further Characterization. Suzanne M.Ramirez. Gregory J. Opiteck, James W. Jorgenson, M. Arthur Moseley III, andRobert J. Anderegg 582

191 Microbore LC/LC/MS for Peptide Mapping and Sequencing. Gregory J. Opiteck.Suzanne M. Ramirez, James W. Jorgenson, M. Arthur Moseley III, and Robert J.

Anderegg 584192 Development of a Novel 2D Microcolumn RPLC-CZE-LIF Assay for [D-

PEN2,5]enkephalin in Rat Serum. Dorothea J. Jeffery and James W. Jorgenson ....

586193 Multidimensional GC-ECD System for the Analysis of CB Marker Congeners.

Russell Kinghorn. Tim Anastasopoulos, Phillip Marriott, and Mark Cumbers 588

XVTTT

194 LC Optimization in the On-line Coupling of LC to GC-ECD. G. Rene van der Hoff.

Ronald Hoogerbrugge, Robert A, Baumann, and Piet van Zoonen 590

195 On-line Clean-up of Soil Samples Prior to GC Analysis. G. Rene van der Hoff.

Peter Kaim, Robert A. Baumann, and Piet van Zoonen 592

196 RPLC-GC with the Vaporizer/Precolumn Solvent Split/Gas Discharge Interface; On¬

line LC-GC-MS Analysis of Phthalates in Water. Tuulia Hyotylainen, Konrad

Grob, Maurus Biedermann, and Marja-Liisa Riekkola 594

197 Statistical Theory of Overlap for Chromatographic Heartcutting. Matthew Peters and

Joe M. Davis 596

198 Multidimensional Capillary GC-GC System for the Analysis of Real Complex Samples.LuigiMondello, Maurizio Catalfamo, Paola Dugo, and

Giovanni Dugo 598

199 Characterization of Petroleum-based Fuels Using Comprehensive Two-dimensional Gas

Chromatography. Richard B. Gaines. Wayne R. Gronlund, and

Glenn S. Frysinger 600

200 Making Quantitative Measurements with Comprehensive Two-dimensional Gas

Chromatography. John B. Phillips and Yang Pan 602

Solid Phase Extraction

201 GC Analysis of Low Volatile Compounds Traces in Water and Air Using Sorbents

with Low Specific Area and Thermodesorption. Irina V. Nesterova, Igor A.

Revelsky. Yuri S. Yashin, Boris I. Zirko, and Maxim G. Lazutin 604

202 Use of Styrene-Divinyl Benzene Copolymer Based Solid Phase Extraction Cartridgesfor General Purpose Air Sampling. Paul J. Clarkson. Malcolm R. Clench, and

Michael Cooke 606

203 Selectivity of Functionalized Silica Solid Phase Extraction Cartridges in Air Sampling.Paul J. Clarkson. Malcolm R. Clench, and Michael Cooke 608

204 Enhanced Recovery of Chlorophenols from Surface Waters Using Polymer Based

Extraction Cartridges. Ricarda Schilling, Paul J. Clarkson. and

Michael Cooke 610

205 GC/MS Determination of Xenobiotics in Fruit Brandy. Zdenek Weidenhoffer and Ivana

Rouckova 612

206 Trace Analysis of Antineoplastic Drugs in Urine of Hospital Pharmacy Technicians

with HRGC/MS. Karlheinz Hauff. Inge Sautter, Gertrud Koethe-Schmolin, and

Angelika Pethran 614

207 Application of On-line SAMOS-HPLC Systems for the Determination of Organic

Compounds in River Water. Ian A. Fowlis. Ivan Jepson, and

Rene J. J. Vreuls 616

208 Separation and Analysis of Produced Formation Water Components. Courtney P.

Milner. Robert D. Trengove, and Iva Stejskal 618

209 Improvement of an SPE Procedure for Analysis of Chlorophenols in Pulp Bleaching

Effluent. Tania Mara. Pissolatto. Patricia Schossler, and

Elina Bastos Caramao 620

Solid Phase Microextraction

210 Application of Solid-phase Microextraction for the Analysis of Pesticides. Ralf Eisert,

Janusz Pawliszyn, and Karsten Levsen 622

211 Automation of On-line SPME Coupled to HPLC. Ralf Eisert and Janusz Pawliszyn 624

212 Advances in Automated Solid Phase Microextraction (SPME). John R. Berg 626

213 Field Portable Fast GC System with Solid Phase Microextraction Interface. Tadeusz

Gorecki. Perry Martos, and Janusz Pawliszyn 628

214 Sample Volume in Solid Phase Microextraction (SPME). Tadeusz Gorecki and Janusz

Pawliszyn 630

215 Strategies for the Analysis of Selected Polar Compounds in Water by SPME. Tadeusz

Gorecki. Perry Martos, and Janusz Pawliszyn 632

216 Determination of Tetraethyllead, Inorganic Lead and Ionic Organolead Compounds in

Water by Solid Phase Microextraction/Gas Chromatography. Tadeusz Gorecki.

Haodan Yuan, and Janusz Pawliszyn 634

217 Solid Phase Microextraction (SPME): Use of SPME for the Determination of Residual

Solvents in Pharmaceutical Drug Substances. Pam Cafiero. Ann-Marie Smith,and Stephen Scypinski 636

218 Determination of Tributyltin and Triphenyltin Compounds in Sediment as Halides bySPME and GC/NICI-MS. Kazuko Mizuishi. Masahiro Takeuchi, and ToshiyukiHobo 638

219 Analysis of Anabolic Steroids from Human Urine and Serum by SPME with On-fiber

Derivatization and GC/MS. Nicholas H. Snow and Pius Okeyo 640

220 Determination of Muscat-type Aroma Additives for Wines Using SPME Head-spaceSampling and CGC Analysis. Gyorgy Vas, A. Lialios, J. Harangi,A. Dobo, and K. V6key 642

221 Determination and Comparison of Volatile Aroma Compounds and Organic Acid

Composition of Various Grape Berries from Eger (Hungary) Using SPME-CGC Technique and HPLC-DAD Analysis. Gyorgy Vas. P. Kozma,J. Harangi, A. Dob6, and K. Vekey 644

222 Solid Phase Microextraction of Flavor Components in Wines. Paola Dugo,Luigi Mondello, and Giovanni Dugo 646

223 Sol-gel Technology for Thermally Stable Coatings in Solid-phase Microextraction.

Sau L. Chong. Brian Wilhite, and Abdul Malik 648

Supercritical and Enhanced Liquid Extraction

224 Optimization of the Experimental Parameters for the Supercritical C02 Extraction ofDicofol Residues from Fish Samples. Fernando M. Lancas. M.S. Galhiane, S.R.

Rissato, and M.A. Barbirato 650225 Determination of PCBs in Water on ppt-ppq Level Using Direct SFE and Whole

Extract Analysis by GC/ECD. Igor N. Glazkov, Igor A. Revelsky. Yuri S.

Yashin, Igor P. Efimov, Boris I. Zirko, and Yuri A. Zolotov 652226 Extraction of Polymer Additives from Polymers by Accelerated Solvent Extraction.

David E. Knowles and Jody Clark 654227 Determination of Fat in Foods Using Accelerated Solvent Extraction (ASE).

Eric S. Francis. David E. Knowles, and Bruce E. Richter 656

YX

228 Understanding the Basis of Analytical Extractions Through the Use of ConceptMapping. Douglas E. Raynie and Richard P. Gregory, IV 658

229 Evaluation of Fluazinan Residue from Fruit Crops by HRGC-ECD. Fernando M.

Lancas. M.A. Barbirato, M.S. Galhiane, and S.R. Rissato 660

230 Comparison of Liquid Extraction and Supercritical Fluid Extraction for the Analysisof Gestagens in Samples of Bovine Fat. Alida A.M. Stolker. Paulus W. Zoontjes,Leendert A. van Ginkel, and Robert J. Maxwell 662

231 Comparison of SFE, Soxhlet and Sonification Methods for the Extraction of OrganicMaterial in Mineral Coal. Maria G.R. Vale. Luiza P. Luz, Ayrton F. Martins,ElinaB. Caramao. Claudio Dariva, and Jose V. de Oliveira 664

232 Subcritical Water Extraction of Organic Pollutants. Yu Yang, Arnaud Lagadec, Maya

Belghazi, David J. Miller, and Steven B. Hawthorne 666

233 Coupled Subcritical Water Extraction/SPME Determination of Organic Pollutants

from Environmental Solids. Kimberly J. Hageman, Carol B. Grabanski, David J.

Miller, and Steven B. Hawthorne 668

234 Extraction of Polar Pollutants Using Enhanced-fluidity Liquids. Susan V. Olesik.

Huimin Yuan, Tricia S. Reighard, and Michael Shows 670

235 Evaluation of Pressurized Fluid Extraction for Use in the Certification of

Environmental Matrix Reference Materials. Michele M. Schantz and Stephen A.

Wise 672

236 Extraction of Polychlorinated Dibenzo-p-Dioxins and Polychlorinated Dibenzo-furans

from Environmental Samples Using Accelerated Solvent Extraction (ASE).

Bruce E. Richter. J.L. Ezzell, D.E. Knowles, F. Hoefler, A.K.R. Mattulat, M.

Scheutwinkel, D.S. Waddell, T. Gobran, and V. Khurana 674

237 On-line SFE-bioassay with MECC of Uterotonically-active Fractions from Medicinal

Plants. Vikash Sewram, Mark W. Raynor. Deshandra M. Raidoo, and Dulcie.A.

Mulholland 676

Novel Extraction/Concentration Techniques238 Membrane Extraction with a Sorbent Interface (MESI): An Efficient and Fast Method

of Clean-up of the Hollow Silicone Membrane. Eduardo Carasek da Rocha,

Fabio Augusto. and Antonio Luis Pires Valente 678

239 Solvent Double-extraction for Sample Clean-up and Preconcentration in a Microliter

Drop System. Minimi Ma and Frederick F. Cantwell 680

240 Synthesis and Use of Molecular Imprints as an Extraction Technique. Richard F. Venn,

Robin Goody, and Barry Kaye 682

241 In-vial Extraction Combined with Large-volume On-column Injection-GC-MS for the

Determination of Anilines in Aqueous Samples at the Low ng/L Level. Ren£

J.J. Vreuls. Edwin Romijn, Thomas Hankemeier, and Udo A.Th. Brinkman 684