SYNTHESIS AND TARGETTING OF

MITOCHINDRIAL PROTEINS

PREPARD BY:

AJAY BADYAL

M.SC. ZOOLOGY

SUBMITTED TO

DEPARTMENT OF ZOOLOGY AND APPLIED AQUACULTURE B.U

SYNPOSIS • INTRODUCTION

• MITOCHONDRIAL PROTEINS.

• PROTEIN TARGETTING IN SORTING.

• SYNTHESIS OF MITOCHONDRIAL PROTEINS.

• CONCLUSION

INTRODUCTION

• Mitochondria are consider as the power house of the cell because in it the energy is liberated and released in the form of ATP which is generated thorough the ADP+Pi.

• It contains their own DNA. Most of the proteins of this organelle are imported to the cytosol after their synthesis. Most of the proteins are synthesized outside the cytosolic ribosome.

• Newly formed proteins are released into the cytosol. The receptors recognise specific uptake targeting sequences in the new proteins.

• Targeting of sub proteins acquires sequential action of two targeting sequences and two membrane bound receptor. One is to direct the protein into the mitochondria & the other to direct the correct organelle or membrane.

• Generally the protein uptake into the mitochondria is an energy requiring process that depends upon the Mito. Membranes.

List of some mitochondrial proteins

Location of proteins in mitochondria Name of the proteins

MATRIX 1. ALCHOL DEHYDROGENASE (YEAST)

2. CARBOXYLIC PHOSPHATE SYNTHETASE

3. CITRATE SYNTHETASE & OTHER CITRIC ACID ENZYMES

4. F1 ATPase SUBUNIT , α,β, & γ

5. ORINTHINE METHYL TRANSFERASE

ORITHININE TRANS CARBOXYLASE

INNER MEMBRANE ADP/ATP ANTIPORTER

CYT.Q CoQH2 (C – REDUCTASE COMPLEX)

SUB UNIT 1,2,5,6,7& 8 Cyt. C OXIDASE SUBUNIT

PHOSPHATE/OH ANTIPORTER

PROTEYOLYTIC OF F0 ATPase

INTER MEMBRANE SPACE Cyt. C

Cyt. C PEROXIDASE

Cyt. B2 & C1 (SUBUNIT OF CQH2- Cyt- REDUCTASE COMPLEX)

OUTER MEMBRANE MITOCHONDRIAL PORINS (H2O TRANSPORT ) P70

SYHTHESIS OF

MITOCHINDRIAL PROTEINS

• Most of the proteins of mitochondria are synthesised in the cytosol.

• All newly formed mitochondrial proteins are formed are initially located in the cytosol.

• Travelling proteins have their each n-terminus residues comprises most targeting sequences.

• The receptor signal sequences are not identical receptor itself recognises such sequences at different destination from mitochondria now are ale to bind through a different but related sequences.

Site of synthesis

PROTEIN TARGETTING IN

SORTING • Mainly most of the proteins of the cytosol or in the

mitochondria are targeting by the two chaperons mitochondrial import stimulation factor (MSF)i.e. HSP-70 and HSP-60.

• The sites from where it goes in side the matrix of the mitochondria is two types TOM-22 & TOM-23 they get further binds up with TOM-70 & TOM-37.

• Targeting while in sorting of proteins is done by the two processes these are CO-translational import and post translational import it occurs at the site where chaperons proteins act n brings proximity an d made a PMF across the membrane.

CONCLUSION • FROM THE ABOVE DISCCUSSION WE

KNOW ABOUT THE MITOCHONDRIAL PROTEINS THEIR SYNTHEISI I N DIFFERENT SITES INSIDE THE ORGANELLE THEN WE KNOW ABOUT THE WAY HOW THEY TARGETTING AT THEIR DESIRED LOCATION/SITES IN THE MITOCHONDRIA OR CYTOSOL AND HOW THEY GENERATES THE ENERGY I NTHE FORM OF PHOSPHATS.

THANKS FOR YOUR ATTENTION…

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