DIAION and SEPABEADSSynthetic Adsorbents

CarminatiRESINDION SRLVia Roma, 55 - 20082 Binasco (MI) - ITALY Phone: +39.02.900130.1 - Fax: +39.02.90090774 - E-mail: marketing@resindion.com

What are Synthetic Adsorbents?

l Spherical crosslinked polymer particle with porous structure

l No ion-exchange groups or functional groups

l Adsorb organic compounds on the surface due to hydrophobic interaction

l Highly porous structure

n Pore diameter :several tens several hundreds angstrom

n High adsorption capacity

n Selective adsorption utilizing size exclusion effect

DIAION HP20

Chemical Structure of Synthetic Adsorbents

l Polystyrenic Adsorbents Standard typen DIAION HP20, HP20SS, HP21

n SEPABEADS SP70, SP700, SP825, SP850

n MCI GEL CHP20A, Y, P, CHP55A, Y

l Chemically Modified Polystyrenic Adsorbentn SEPABEADS SP207

Higher adsorption capacityHigh gravity (1.2) Applicable to Expanded Bed Adsorption

or Countercurrent operation

l Polymethacrylic Adsorbentn DIAION HP2MG

n MCI GEL CHP2MG, CHP2MGYMore polar than polystyrenic resinsApplicable to normal-phase adsorption

BrBr

Br

BrBr

Br

CH3 CH3

CH3CO

O

CH2

CH2

O

CO CH3

CH3CH3

C O

C O

OCH3

OCH3

C O

OCH3

C O

OCH3

What Kind of Compounds can be Separated?

l Antibiotics from Fermentation Broth

l Herbal Extracts

l Peptides and Proteins

l Vitamins

List of Industrial Synthetic Adsorbents

1.21.31.31.12.31.61.41.2Pore volume

(ml/g)

1701052608090705738Pore radius

(Angstrom)

SEPABEADS SP825

DIAION HP20

DIAION HP21

SEPABEADS SP850

Cumulative pore volume

Logarithmic differential

Peptides, Proteins - LactamsMacrolides

SEPABEADS SP70SEPABEADS SP700

Pore radius

Por

e vo

lum

e

mL/

g

Pore Size Distribution of Synthetic Adsorbents

Adsorption

Washing

Elution

Regeneration

How to Use Synthetic Adsorbents

l Column Adsorption Method

l Chromatographic Operation

Concentration

Tim

e

Column

Adsorbent

Interaction between adsorbent and molecules

Method Development of Chromatographic Separation

l HPLC separation using analytical columnsn Selection of adsorbent type polystyrenic or polymethacrylic

n Selection of eluent system

l Scale up separation using preparative columnsn Effect of particle sizes on separation

n Effect of loading amount on separation

n Adsorbent life, Regeneration method, Feasibility study

n Equipment design(Single or multi column system, SMB system)

l Industrial Operationn Fine adjustment of conditions previously determined

FractionCollector

Detector

Colum

n

Colum

n Oven

Example of HPLC Analysis Equipment

l Column sizen CHP10M, CHP2MGM ( 4m)

150 x 4.6mm I.D.- Resin volume: 2.50ml

n CHP5C, CHP2MG (10m)250 x 4.6mm I.D.- Resin volume: 4.15ml

l Example of flow raten 150 x 4.6mm I.D.

- 0.5ml/min: LV=1.8m/h, SV=12

n 250 x 4.6mm I.D.- 1.0ml/min: LV=3.6m/h, SV=14.4

SampleInjector

Regenerant

FeedSolution

Colum

n

FractionCollector

DetectorPump

Example of Preparative Chromatography Equipment

l Example of preparative columnsn 500 x 20mm I.D.

- Resin volume: 157ml

n 500 x 90mm I.D.- Resin volume: 3,180ml

l Example of flow rate n 500 x 20mm I.D.

- 6.28ml/min:LV=1.2m/h, SV=2.4

n 500 x 90mm I.D.- 63.6ml/min:

LV=0.60m/h, SV=1.2

Eluent

Water

Example of Packed Columns

Analytical columnCHP10M

150 x 4.6mm I.D.

Preparative column 1CHP55A

500 x 32mm I.D.(Max. Vol.: 400mL)

Preparative column 2CHP55Y

500 x 90mm I.D.(Max. Vol.: 3L)

Methods of Adsorption and Elution

l Solvent Composition Changen Adsorption Process

Aqueous solution having the lowest solvent content

n Elution ProcessPure solvent or Aqueous solution with high solvent composition

l pH Changen Adsorption Process

Aqueous solution adjusted at pH region in which product ionic functionality does not dissociate

n Elution ProcessLow medium buffer solution adjusted at pH region in which product ionic functionality dissociates

Pressure Drop of Synthetic Adsorbents

HP20

HP21

SP207

SP825

Hydraulic Properties (Water, 25C)

0

0.02

0.04

0.06

0.08

0.1

0 10 20 30 40 50

Linear Velocity (m/hr)

Hea

d Lo

ss (

MP

a/m

-bed

)

Swelling / Shrinkage of Synthetic Adsorbents

Swelling ratios of HP20 for variousaqueous solvents (25C)

1

1.1

1.2

1.3

1.4

0 20 40 60 80 100Solvent concentration (v/v %)

Sw

ellin

g R

atio

MeOH

EtOH

IPA

Acetone

AcCN

Swelling ratios of HP2MG for variousaqueous solvents (25C)

1

1.1

1.2

1.3

1.4

0 20 40 60 80 100Solvent concentration (v/v %)

Sw

ellin

g R

atio

MeOH

IPA

Advantages of Using Synthetic Adsorbents

l From Analytical to Industrial Separationsn With the use of same polymer structure performances

n With same separation conditions

l Chemical Stabilityn Stable in a wide pH range

l Cost Effectivenessn Long operation life

n Simple regeneration method with chemical at low ecological impact

Itochu Specialty Chemicals

RESINDION MITSUBISHICHEMICAL

TAI-YOUNGCHEMICAL

Manufacturing / Sales divisionSales divisionSales representative

MCHK

RENSUI ASIA http://www.diaion.com.tw/http://www.diaion.com.tw/

http://www.diaion.com/http://www.diaion.com/

http://www.itochuhttp://www.itochu--sc.com/sc.com/

http://www.resindion.com/http://www.resindion.com/

Worldwide Network of DIAION Worldwide Network of DIAION / MCI / MCI GELGEL

DIAION AND SEPABEADS SYNTHETIC ADSORBENTS.pdfDIAION AND SEPABEADS