An Introduction to Synthetic Biology

“Hacking DNA for Fun and Profit”

1. what?2. applications3. the tech4. free and safe5. go hack

1. what?2. applications3. the tech4. free and safe5. go hack

Engineeringvs Science

Science Engineering

Artifacts(cells)

Understanding,Models

Synthetic Biology

Synthetic Biology=

GeneticEngineering++

1. Recombinant DNA2. PCR3. Automated sequencing

1. Recombinant DNA2. PCR3. Automated sequencing4. Automated construction5. Standardization6. Abstraction

Implementation Modelling

Design

Specifications

Testing/Validation

Standard

Engineering

Practice

The Imperial College of London, iGEM 2006

Specifications Design Implementation TestingModelling

Specifications oscillator

Based on Lotka-Volterra

Modelling Lotka-Volterra

Based on Quorum Sensing

ModellingFull system

Specificationstest constructs

Designtest constructs

ModellingTest construct

Buildtest constructs

Characterizedtest constructs

Modelling characterized sys

Build fullsystem

CharacterizedFull system Our Goal!

Start!

The Imperial College of London, iGEM 2006

1. what?2. applications3. the tech4. free and safe5. go hack

Biofuels

Bioremediation

Medicine

Space ISRU

1. what?2. applications3. the tech4. free and safe5. go hack

http://upload.wikimedia.org/wikipedia/commons/e/eb/Epson-inkjet-printer.jpg

$1/bp, 4wk

e. coli: 4kyou and me: 3gbp

StandardziedAssembly

CommonSignal Carrier

PolymeraseOperationsper Second

Characterization

Composition& Abstraction

http://www.ccbi.cam.ac.uk/iGEM2006/index.php/Description

http://www.ccbi.cam.ac.uk/iGEM2006/index.php/Description

1. what?2. applications3. the tech4. free and safe5. go hack

Legal Frameworks

Human Factors

1. what?2. applications3. the tech4. free and safe5. go hack

http://www.igem.org

http://parts.mit.edu

http://www.openwetware.org

Questions!

diybio.orgigem.org

parts.mit.eduopenwetware.org

biohack.sf.net