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1Rapid Solutions for Food Safety

Verify Cleaning and

Monitor Hygiene

ATP bioluminescence (Rapid & Alternative Method)


Hygiene Monitoring Overview• History• ATP bioluminescence

– a rapid alternative method• Why is cleaning important• Who does rapid hygiene testing?• How is it done?• When should it be done?• Where to sample ?• Benefits

2Hygiene Monitoring Solutions Ltd Tel: 01327 349130


History

• Visual inspection is inadequate• Plate counts

– Detects only microbes– Requires skilled analysts in labs– Expensive– Slow – Tells nothing about the removal of

dirt and organic matter



Rapid Alternative Method ATP Bioluminescence

• ATP = Adenosine Triphosphate– Universal energy carrier – Present in all living things and organic matter (dirt and microbes)

• Bioluminescence– Natural reaction producing light – Specific enzyme reacts only with ATP– Results in seconds– Light is measured by an instrument and gives a numerical output

• Simple Test Method – ATP collected by a swab sample reacts with a test reagent – Light detected by the instrument– High ATP = High contamination– Low ATP = Low contamination



Who does Rapid Hygiene Testing?

• Large food and drink producers and processors

• Smaller food manufacturers and materials suppliers

• Caterers in restaurant and hospital kitchens

• Manufacturers of Personal Care Product• Auditors, inspectors, consultants etc.



What is required?

• Instrument – SystemSURE Plus

• Reagent swab device – Ultrasnap



• Palm-sized instrument

• Light weight (less than 300 g)

• Runs up to 6 months with 2 AA Penlights

• Stores 2000 test results

• 250 Programmable locations

• 20 Test Plans

• Thresholds set from instrument

• Simple operation

• Excellent sensitivity in combination with

Ultrasnap test device

• USB Smart Cable connectivity

• SureTrend

• Results Upload Utility (Excel)

Low cost luminometer dedicated for Hygiene Monitoring

SystemSURE Plus Luminometer



Procedure

Ultrasnap

• All-in-one swab device

• Ready-to-use

• Suitable for solid surfaces and for rinse water

• Simple procedure: Swab, Snap, Squeeze & Read

1

Bulb

Snap Valve

Swab Shaft

Swab Tip

Swab Tube

Swab

2

Snap

3

Squeeze Read

4



When to test?

• Pre-rinse (optional)

• Detergent clean

• Detergent rinse

• Disinfection (optional)

Fail

Pass



Where to test?

• Conduct a hazard analysis & risk assessment to identify critical areas based on – Type of Product residue– Processing equipment – Cleaning duty required– Potential for cross-contamination



Standards & Targets• No universal Pass / Fail limits

– Surfaces vary in form, function, contamination risk and cleaning duty

– Compare before and after cleaning• General guidance for SystemSURE Plus

– Pass < 20 RLU – Caution 21 - 49– Fail > 50

• Higher standards e.g. Pass < 10 and Fail > 30• Trend analysis and continuous review to improve

standards that are achievable.



Benefits of Rapid ATP test• Verify cleaning has been achieved

– Direct objective test for removal of organic matter– Better than visual inspection – Better than traditional microbiological methods

• Identify high risk vulnerable areas• Provide rapid result (15 sec) for

– on-the-spot information– Immediate corrective action e.g. re-clean

• Evidence of due diligence • Provides objective data

– trend analysis and continuous improvement


Date post:	01-Jul-2015
Category:	Technology
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