Table 1: Main characteristics of the most used NGS technologies in omics studies.

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Input material

Genomic DNA RNA PCR amplified DNA

Reverse transcriptionfragmentation

Linear DNA molecules

Adapter Ligation

DNA library

cDNA

Attachment to

microbead

Attachment to glass

slide

Emulsion PCR

Bridge PCR

Deposition in

PTP or Chip

Hybridization to glass slide

Detection of light

or pH shift

Detection of

fluorescence

454 and Ion

TorrentSOLiD Illumina

Seq

uen

cin

gL

ibrary a

mp

lifi

cati

on

Lib

rary p

rep

arati

on

Fig. 1: Schematic presentation of the library preparation, amplification and sequencing process of the most commonly used next generation sequencing platforms (Adapted from Knief et al. 2014)

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Table 2: Studies of the plant-associated microbiota using next-generation sequencing.

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