ITP 2010 Final Program Page | 1 Table of Contents Page Table of Contents 1 General Information 2-3 Poster Session Guidelines 3 Proceedings 3 Committees 4 Past Symposia in the Series 4 Sponsors 5 Travel Award Recipients 5 Scientific Program 6-35 List of Exhibitors and Sponsors 36-37 Floor Plan of Meeting Space end of program The use of cameras and cell phones are not permitted during program sessions. Cameras are permitted on the Exhibit floor; however, permission from the exhibitors involved must be obtained before photographs can be taken. Opinions expressed by individuals presenting abstracts and workshops are not necessarily the opinions of the ITP 2010 Symposium
Transcript
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 1
Table of Contents
Page
Table of Contents 1
General Information 2-3
Poster Session Guidelines 3
Proceedings 3
Committees 4
Past Symposia in the Series 4
Sponsors 5
Travel Award Recipients 5
Scientific Program 6-35
List of Exhibitors and Sponsors 36-37
Floor Plan of Meeting Space end of program
The use of cameras and cell phones are not permitted during program sessions. Cameras are permitted on the Exhibit floor; however, permission from the exhibitors involved
must be obtained before photographs can be taken.
Opinions expressed by individuals presenting abstracts and workshops are not necessarily the opinions of the ITP 2010 Symposium
Beckman Coulter is determined to enhance your CE experience by developing key technologies and providing you solutions to help optimize your workfl ow and effi ciency. From the highly sensitive multiplex gene expression capabilities of the GeXP to the high-resolution applications of the NEW PA 800 plus Pharmaceutical Analysis System, Beckman Coulter unlocks the power of best-in-class performance. Select from existing panels for biomarker validation and pathogen detection, or request customized multiplexes for cancer research and toxicity studies. Choose validated methods for cIEF, CE-SDS or glycan analysis, or develop custom separations using the powerful 32 KaratTM software. As the leader in CE technology, we provide solutions for the separation, identifi cation, quantitation and characterization of many analytes, including ions, nucleic acids, basic drugs, enantiomers, proteins and carbohydrates. Our development initiative encompasses hardware, software and chemistry, with a long list of advancements including our NEW PA 800 plus Pharmaceutical Analysis System, sheathless CE-MS, and GeXP Genetic Analysis System.
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 2
General Information VENUE Tremont Grand
222 St. Paul Street Baltimore, MD 21202, USA Phone: 410-727-2222 www.tremont.com
MEETING ROOMS Corinthian Room, 2nd Floor
Take Hotel Elevator to the 5th Floor **You are now on the 2nd Floor of the Tremont Grand** Walk through the Roman Strada Room Make the Forced Left to Find Corinthian Room
3rd, 4th and 5th Floors of the Tremont Grand Take Hotel Elevator to the 5th Floor Walk down Hallway (Skywalk) **You are now on the 2nd Floor of the Tremont Grand** Take Silver Elevators on the left to the 3rd, 4th and 5th Floors
BADGES A name badge must be worn by each registered participant and accompanying
person in order to gain admittance to the meeting and social gatherings. REGISTRATION Symposium Registration Desk is located on 2nd Floor of the Tremont Grand. EXHIBITS The exhibition is an important component of the meeting, so please take the
time to thank the exhibitors for their support of the program. Exhibits, poster sessions, sunday reception, and coffee breaks are located on 5th Floor of the Tremont Grand. Exhibit Hours:
Sunday 4:30 PM − 8:00 PM Monday 9:00 AM − 6:00 PM Tuesday 9:00 AM − 6:00 PM
SOCIAL Sunday Welcome Reception 7:00 PM – 8:00 PM (free to all registrants) Tuesday Conference Dinner 7:30 PM (TICKET REQUIRED)
A small number of dinner tickets may be available at the Symposium Registration Desk
FREE VENDOR WORKSHOPS – must register with vendor in order to attend Monday Agilent Electrophoresis Lunch & Learn Workshop
You must sign up to attend the Free Vendor Workshop by contacting Must Register Agilent Technologies. A complimentary lunch will be provided during the to attend workshop for those who sign up. Tuesday Developments in Capillary Electrophoresis at Beckman Coulter
You must sign up to attend the Free Vendor Workshop by contacting Must Register Beckman Coulter. A complimentary lunch will be provided during the to attend workshop for those who sign up.
Editor-in-Chief
Ziad El Rassi, Stillwater, OK, USA
Senior Deputy Editor
Petr Bocek, Brno, Czech Republic
Deputy Editors
František Foret, Brno, Czech Republic
Bohuslav Gaš, Prague, Czech Republic
Jong Hoon Hahn, Pohang, South Korea
Jörg P. Kutter, Lyngby, Denmark
Bingcheng Lin, Dalian, China
Takashi Manabe, Matsuyama, Japan
Terry M. Phillips, Bethesda, MD, USA
Andreas M. Rizzi, Wien, Austria
Nancy C. Stellwagen, Iowa City, IA, USA
Victor M. Ugaz, College Station, TX, USA
Founding Editor
Bertold J. Radola, Munich, Germany
ELECTROPHORESIS is the leading journal for new analytical and preparative electrophoretic methods and innovative applications. Continuously striving to provide readers and authors with the best platform for communicationFeatures include:
Fast Track for rapid publication of papers describing important/breakthrough research results
“Emphasis on …” sections featuring a series of review and research papers that belong to a specifi c topic
1540
6
ELECTROPHORESIS
www.electrophoresis-journal.com
To read a free sample copy or to set e-mail alert visit
Editor-in-Chief
Ziad El Rassi,
Senior Deputy Editor
Petr Bocek,
Deputy Editors
František Foret,
Bohuslav Gaš,
Jong Hoon Hahn,
Jörg P. Kutter,
Bingcheng Lin,
Takashi Manabe,
Terry M. Phillips,
Andreas M. Rizzi,
Nancy C. Stellwagen,
Victor M. Ugaz,
Founding Editor
Bertold J. Radola,
ELECTROPHORESIS is the leading journal for new
ELECTROPHORESISImpact Factor:
3.509 *
(*Thomson Reuters Citation Report 2008)
Special Issues in 2010 CE and CEC Reviews
Fundamentals
CE-MS
CEC and EKC
Bioanalysis
Food Analysis
Microcolumn Separation Methods for Metabolomics
CE and CEC Innovations
Instrumentation in Chip and CE
Miniaturization in Asia Pacifi c
Miniaturization 2010
Volume 31. 24 Issues in 2010ISSN Print: 0173-0835ISSN Online: 1522-2683
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 3
General Information (continued) POSTER Poster Sessions are located on 5th Floor of the Tremont Grand. SESSIONS
All posters will be put up on Monday and should remain up on the poster board during the entire meeting no matter what day the poster will be presented. Poster boards are labeled with the number corresponding to the abstract number in the Scientific Program.
Presenters must be in attendance at their poster board on the day of their poster session:
Monday Poster Presentations: P-100 and P-200 Tuesday Poster Presentations: P-300 and P-400 Wednesday Poster Presentations: P-500 and P-600
Poster Set-up: All posters are to be put up on Monday, August 30, between 7:30AM and 1:00PM. Poster Tear-down: Remove only on Wednesday between 3:00PM and 6:00PM. Anything remaining after 6:00PM will be discarded. Be sure to check the reprint envelope daily where people leave business cards to request reprints of your abstract.
PROCEEDINGS Each lecture and poster presenter is encouraged to submit a manuscript to the
journal, Electrophoresis, for inclusion in the Proceedings of ITP 2010. Note that each manuscript will be reviewed as normal following the policy of the Journal. Manuscripts should be submitted through the Electrophoresis web site at http://mc.manuscriptcentral.com/elpho by no later than October 31. The issue will be in print in March 2011.
MESSAGES Located on 2nd Floor of the Tremont Grand near the Symposium Registration
area will be a board for messages as well as for posting notices of positions available and positions wanted.
Scientific, National and International Committees Scientific Committee B. Chankvetadze* (Tbilisi, Georgia) Z. El Rassi* (Stillwater, OK, USA)—Chairman S. Fanali* (Rome, Italy) F. Foret* (Brno, Czech Republic) B. Gas* (Prague, Czech Republic) T. Hirokawa* (Hiroshima, Japan) S. Hjerten* (Uppsala, Sweden) D. Kaniansky* (Bratislava, Solvakia) deceased V. Kasicka* (Prague, Czech Republic) E. Kenndler* (Vienna, Austria) * permanent members
National Organizing Committee A. Barron (Stanford, CA, USA) J. Chapman (Fullerton, CA, USA) H. Issaq (Frederick, MD, USA) C.S. Lee (College Park, MD, USA) T. Phillips (Bethesda, MD, USA) G. Vigh (College Station, TX, USA) International Organizing Committee J. Bergquist (Uppsala, Sweden) A. Cifuentes (Madrid, Spain) F. Foret (Brno, Czech Republic) B.-C. Lin (Dalian, China) K. Otsuka (Kyoto, Japan) G. Rozing (Waldbronn, Germany)
Past Symposia and Chairs 1979 Baconfoy (Belgium) A. Adam and C. Schots 1980 Eindhoven (The Netherlands) F.M. Everaerts 1982 Gosslar (Germany) C.J. Holloway 1984 Hradec Kralove (Czechoslovakia) Z. Prusik 1986 Maastricht (The Netherlands) F.M. Everaerts 1988 Vienna (Austria) E. Kenndler 1990 Tatranska Lomnica (Czechoslovakia) D. Kaniansky 1992 Rome (Italy) S. Fanali 1994 Budapest (Hungary) F. Kilar 1996 Prague (Czech Republic) B. Gas 1998 Venice (Italy) P.G. Righetti 2000 Bratislava (Slovak Republic) - Vienna (Austria) D. Kaniansky and E. Kenndler 2002 Helsinki (Finland) M.L. Riekkola 2004 Rome (Italy) S. Fanali and M.G. Quaglia 2006 Paris (France) G. Peltre 2008 Catania (Italy) V. Cucinotta 2
17th International Symposium on Electro- and Liquid Phase-separation Techniques
(International Symposium on Capillary Electroseparation Techniques)
Symposium Chairman Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
Sunday, August 29, 2010 – Courses, Exhibits, Plenary Lectures, Reception Course 1: CAPILLARY ELECTROPHORESIS FOR BIOMOLECULES Time: 9:00 am - 5:00 pm Location: Tuscan Room, 3rd Floor Instructor: Robert Weinberger, Ph.D., CE Technologies, Inc., Chappaqua, NY, USA Course 2: CE-MS: PRINCIPLES AND APPLICATIONS Time: 9:00 am - 4:00 pm Location: Composite Room, 3rd Floor Instructors: Prof. Gerhardus (Ad) de Jong, University Utrecht, Utrecht, THE NETHERLANDS Prof. Christian W. Klampfl, Johannes Kepler University, Linz, AUSTRIA
Prof. Alejandro Cifuentes, National Research Council (CSIC), Madrid, SPAIN 4:30-7:00 pm SYMPOSIUM REGISTRATION (2nd Floor) 4:30-8:00 pm EXHIBITION OPEN (5th Floor)
5:25 pm Remarks by the Chair of ITP 2010 Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
SESSION 1. OPENING PLENARY LECTURES LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chair: Jeff Chapman, Beckman Coulter Inc., Brea, CA, USA
5:40 pm L-101 The Essential Role of Mass Spectrometry in Capillary Separation Techniques. Barry L. Karger, Barnett Institute, Northeastern University, Boston, MA, USA [PLENARY LECTURE]
6:20 pm L-102 The Proteome Buccaneers: How to Unearth Your Treasure Chest via Combinatorial
Peptide Ligand Libraries. Pier Giorgio Righetti, E. Boschetti, Politecnico di Milano, Milano, ITALY [PLENARY LECTURE]
7:00-8:00 pm WELCOME RECEPTION IN THE EXHIBIT HALL (5th Floor)
Sponsored in part by Beckman Coulter
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 7
Monday, August 30, 2010 – Plenary Lectures & Morning Parallel Sessions 7:30 am SYMPOSIUM REGISTRATION OPEN (2nd Floor) 9:00am-6:00pm EXPOSITION OPEN (5th Floor)
SESSION 2. PLENARY LECTURES LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chair: Barry Karger, Northeastern University, Boston, MA, USA
8:00 am L-103 Micro- and Nanofluidics for Single Biomolecule and Single Cell Analysis. Yoshinobu Baba, Nagoya University, Nagoya, JAPAN [PLENARY LECTURE]
8:30 am L-104 Lab on a Chip: A Key Platform for Biomedical Study and Diagnosis. Bingcheng Lin, Jianhua Qin, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Liaoning, CHINA [PLENARY LECTURE]
9:00 am BREAK in Exhibit Hall (5th Floor)
SESSION 3A. PROTEOMICS 1 LOCATION: CORINT IAN ROOM (2nd Floor) HSession Chairs: Jun Hirabayashi, Research Center for Medical Glycoscience, Umezono, Tsukuba, JAPAN, and Iulia Lazar, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
9:30 am L-105 Expanding the Proteome Coverage of Organelles and Cells: Applications in Cardiac
Disease. Melanie White, Rebekah Gundry, Vidya Venkatraman, Jennifer E Van Eyk, Johns Hopkins University, Baltimore, MD, USA [KEYNOTE LECTURE]
10:00 am L-106 Catching the Brain Membrane Protein Train - A Novel Strategy for Enrichment and
Analysis. Ganna Shevchenko, Marcus O. D. Sjödin, David Malmström, Magnus Wetterhall, Jonas Bergquist, Uppsala University, Uppsala, SWEDEN
10:25 am L-107 Quantitative Nan Proteomics for Protein Complex (QNaoPX) Approach using Diverse o
Affinity Chemical Probes. Shu-Hui Chen, National Cheng Kung University, Tainan, TAIWAN 10:50 am L-108 Capillary Isotachophoresis-based Tissue Proteome Platform. Xueping Fang1, Tong
Guo1, Kejia Zhao1, Brian M. Balgley2, Weijie Wang2, Cheng S. Lee1, 1University of Maryland, College Park, MD, USA; 2Calibrant Biosystems, Gaithersburg, MD, USA
11:15 am Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 8 Monday, August 30, 2010 – Morning Parallel Sessions
SESSION 3B. ENANTIOSEPARATIONS LOCATION: DORIC ROOM (4th Floor) Session Chairs: Gyula Vigh, Texas A & M University, College Station, TX, USA, and Pavel Kubáň, Institute of Analytical Chemistry, Brno, CZECH REPUBLIC
9:30 am L-109 Capillary Electrophoresis Applied to Enantiomer Separation and Estimation of Helix
Inversion Barrier of Cationic Helicene-like Species. Vaclav Kasicka, D. Koval, P. Sazelova, L. Severa, L. Adriaenssens, F. Teply, Academy of Sciences of the Czech Republic, Praha, CZECH REPUBLIC [KEYNOTE LECTURE]
10:00 am L-110 Mechanistic Aspects of Enantioseparations in Capillary Electrophoresis. Bezhan Chankvetadze, Tbilisi State University, Tbilisi, GEORGIA
10:25 am L-111 Capillary Electrophoresis-Mass Spectrometry: Innovative Methodologies for Sensitive
Analysis of Chiral Compounds. Shahab A. Shamsi, Jun He, Georgia State University, Atlanta, GA, USA
10:50 am L-112 The Use of Derivatized Cyclofructans as Novel Chiral Selectors in CE and HPLC.
Zachary S. Breitbach, Ping Sun, Daniel Armstrong, The University of Texas at Arlington, Arlington, TX, USA
11:15 am Pause
SESSION 3C. AFFINITY ELECTROPHORESIS (session organized by Terry Phillips) LOCATION: COMPOSITE ROOM (3rd Floor) Session Chair: Terry Phillips, NIH, Bethesda, MD, USA
9:30 am L-113 Capillary Electrophoresis-Based Immunoassays: An Overview of Principles and Quantitative Applications. David S. Hage, Annette C. Moser, University of Nebraska-Lincoln, Lincoln, NE, USA, and University of Nebraska-Kearney, Kearney, NE, USA [KEYNOTE LECTURE]
10:00 am L-114 Combining Affinity CE and Taylor Dispersion Analysis (Nanosizing) for Efficient Protein
Characterization. Jesper Østergaard, Henrik Jensen, University of Copenhagen, Copenhagen, DENMARK
10:25 am L-115 Microfluidic Western Blotting. Amy E. Herr, University of California, Berkeley, CA, USA 10:50 am L-116 ACE with Proteins: Precise Characterization of Complex Binding Behaviour.
Deia El-Hadya, Sascha Kühneb, Adhitasari Suratmanb, Michael Grafb, Nagwa El-Maalia, Hermann Wätzigb, aAssiut University, Assiut, EGYPT; bTU Braunschweig, Braunschweig, GERMANY
11:15 am Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 9 Monday, August 30, 2010 – Morning Parallel Sessions
SESSION 4A. MULTIDIMENSIONAL APPROACHES IN MEMORIAM OF THE LATE PROF. DUSAN KANIANSKY LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: Pier Giorgio Righetti, Politecnico di Milano, Milano, ITALY, and Salvatore Fanali, Institute of Chemical Methodologies, Monterotondo Scalo, ITALY
11:20 am L-117 A Column-switching Tool by Joining CZE with ITP while Benefitting for Suppressed
Laminar and Electroosmosis Flows. Dušan Kaniansky, Róbert Bodor, Marián Masár, Comenius University, Bratislava, SLOVAKIA
11:45 am L-118 Potential of Two-Dimensional Electro-Fluid-Dynamic Devices for Continuous Chemical
Purification. Chang Liu1, Yong Lou2, E. Jane Maxwell1, Ning Fang2, David D. Y. Chen1, 1University of British Columbia, Vancouver, CANADA; 2Iowa State University, Ames, IA, USA
12:10 pm L-119 Improving Detection Sensitivity of ITP-CE in Medical Diagnostic Applications.
L. Bousse1, W. W. P. Chang1, Ring-Ling Chien1, C. Li1, T. Kawabata2, S. Satomura2, H.G. Wada1, 1Wako Pure Chemical Industries, Ltd., Mt. View, CA, USA; 2Wako Pure Chemical Industries, Ltd., Osaka, JAPAN
12:30 pm Pause – Lunch on own 12:45-1:45 pm Free Vendor Workshop – Must register to attend free workshop
Agilent Electrophoresis Lunch & Learn Workshop LOCATION: TUSCAN ROOM (3rd Floor) You must sign up to attend the Free Vendor Workshop by contacting Agilent Technologies. A complimentary lunch will be provided during the workshop for those who sign up.
1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-100 and P-200 series of posters presented
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 10 Monday, August 30, 2010 – Morning Parallel Sessions
SESSION 4B. VARIOUS OMICS LOCATION: DORIC ROOM (4th Floor) Session Chairs: Thomas Laurell, Lund University, Lund, SWEDEN, and James Landers, University of Virginia, Charlottesville, VA, USA
11:20 am L-120 Recent Applications of Capillary Electromigration Methods in Foodomics. Carolina
Simo, Virginia Garcia-Cañas, Clara Ibañez, Miguel Herrero, Alejandro Cifuentes, IFI, Madrid, SPAIN
11:45 am L-121 Separation Capillary as a Microreactor: A Proof of Principle in In vitro Metallomic
Studies by CE. Andrei R. Timerbaev1, L. S. Foteeva1, J. K. Abramski2, K. Pawlak2, M. Jarosz2, 1Vernadsky Institute of Geochemistry and Analytical Chemistry, Moscow, RUSSIA; 2Warsaw University of Technology, POLAND
12:10 pm L-122 2-D Gel-based Proteome Profiling of Proteins Expressed in Cultured 46BR.1G1 Cells
Established from a Patient Affected by DNA Ligase I Deficiency Syndrome: A Pilot Study. Serena Giuliano1, Roberta Salvini1, Paolo Iadarola1,Valentina Leva2, Alessandra Montecucco2, Serena Camerini3, Marco Crescenzi3, Anna Bardoni1, 1Università di Pavia, ITALY; 2Istituto di Genetica Molecolare, Pavia, ITALY; 3Istituto Superiore di Sanità, Roma, ITALY
12:35 pm Pause – Lunch on own 12:45-1:45 pm Free Vendor Workshop – Must register to attend free workshop
Agilent Electrophoresis Lunch & Learn Workshop LOCATION: TUSCAN ROOM (3rd Floor) You must sign up to attend the Free Vendor Workshop by contacting Agilent Technologies. A complimentary lunch will be provided during the workshop for those who sign up.
1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-100 and P-200 series of posters presented
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 11 Monday, August 30, 2010 – Morning Parallel Sessions
SESSION 4C. APPLICATIONS AND METHODOLOGIES 1 LOCATION: COMPOSITE ROOM (3rd Floor) Sessions Chairs: Reinhard H. H. Neubert, Martin Luther University, Halle (Saale), GERMANY and Michael T. Bowser, University of Minnesota, Department of Chemistry, Minneapolis, MN, USA
11:25 am L-123 A Microemulsion Electrokinetic Chromatography (MEEC) Method for Analysis of
Microcystins in Water. Grace Birungi, Sam F. Y. Li, National University of Singapore, Singapore, SINGAPORE
11:45 am L-124 Capillary Electrophoretic Methods for Food Toxins Generated from Natural Food
Ingredients. F. Bedia Erim, Istanbul Technical University, Department of Chemistry, Maslak, Istanbul, TURKEY
12:10 pm L-125 Application of Sheathless CE-MS for the Analysis of Posttranslationally Modified
Peptides. Herbert Lindner, Klaus Faserl, Leopold Kremser, Bettina Sarg, Innsbruck Medical University, Innsbruck, AUSTRIA
12:30 pm Pause – Lunch on own 12:45-1:45 pm Free Vendor Workshop – Must register to attend free workshop
Agilent Electrophoresis Lunch & Learn Workshop LOCATION: TUSCAN ROOM (3rd Floor) You must sign up to attend the Free Vendor Workshop by contacting Agilent Technologies. A complimentary lunch will be provided during the workshop for those who sign up.
1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-100 and P-200 series of posters presented Monday, August 30, 2010 – Afternoon Parallel Sessions
SESSION 5A. BIOMARKERS LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: Jennifer E Van Eyk, Johns Hopkins University, Baltimore, MD, USA, and Philip Britz-McKibbin, McMaster University, Hamilton, ON, CANADA
3:00 pm L-126 Disease Biomarker Discovery: Opportunities and Pitfalls. Haleem J. Issaq, Josip
Blonder, Timothy D. Veenstra, SAIC-Frederick, Inc., Frederick, MD, USA 3:20 pm L-127 Assessment of a Modular, Multi-task Device Connected to Capillary Electrophoresis
and Other Chromatographic Instruments for the Enrichment, Separation and Identification of Protein Biomarkers. Norberto A. Guzman, Princeton Biochemicals Inc., Princeton, NJ, USA
3:40 pm L-128 Microfluidic Chip-Capillary Electrophoresis for Emergent Clinical Diagnosis and
Monitoring of Biomarkers and Metabolites. Ying Sing Fung, Hong Kong University, Hong Kong, CHINA
4:00 pm Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 12 Monday, August 30, 2010 – Afternoon Parallel Sessions
SESSION 5B. SAMPLE PREPARATION AND ENRICHMENT LOCATION: DORIC ROOM (4th Floor) Session Chairs: Oliver Trapp, Ruprecht-Karls-Universität Heidelberg, Heidelberg, GERMANY, and David Ross, National Institute of Standards and Technology, Gaithersburg, MD, USA
3:00 pm L-129 Electric Field Enhanced Transport Across Phase Boundaries and Membranes for
Sample Pretreatment in Bioanalysis. Pavel Kubáň1, Lenka Langová1, Oanh Doan Thi Kieu2, Petr Gebauer1, Petr Kubáň2, Petr Boček1, 1Academy of Sciences of the Czech Republic, Brno, CZECH REPUBLIC; 2Mendel University, Brno, CZECH REPUBLIC
3:20 pm L-130 Capillary Isoelectric Trapping of Proteins and Peptides: Submicroliter-volume
Fractionation, Desalting, Enrichment and Digestion. Ken K.-C. Yeung, University of Western Ontario, London, CANADA
3:40 pm L-131 Transient Isotachophoretic Stacking in Zone Electrophoresis – Benefits and Risks. Petr
Gebauer, Zdenka Mala, Petr Bocek, Ludmila Krivankova, Academy of Sciences of the Czech Republic, Brno, CZECH REPUBLIC
4:00 pm Pause
SESSION 5C. METHODOLOGIES LOCATION: COMPOSITE ROOM (3rd Floor) Sessions Chairs: W. Mike Arnold, MacDiarmid Institute at Industrial Research Ltd, Lower Hutt, NEW ZEALAND, and Koji Otsuka, Kyoto University, Kyoto, JAPAN
3:00 pm L-132 Capillary Zone Electrophoresis using Electrokinetic Supercharging Preconcentration –
From Small Ions to DNA Fragments. Takeshi Hirokawa, Zhongqi Xu, Kentaro Nakamura, Kouji Kawahito, Mihoro Yamada, Hiroshima University, Higashi-hiroshima, JAPAN
3:20 pm L-133 Chip-based Receptor Affinity CE for Measuring Bioactive Inflammatory Cytokines in
Human Biopsies. Heather Kalish, Terry Phillips, Edward Wellner, National Institute of Biomedical Imaging and Bioengineering, Bethesda, MD, USA
3:40 pm L-134 New Approaches in Pharmaceutical Analysis by LC-ESI-IT-MS. Zilin Chen, Qinhua
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 13 Monday, August 30, 2010 – Afternoon Parallel Sessions
SESSION 6A. AFFINITY AND INTERACTIVE ELECTROPHORESIS LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: David S. Hage, University of Nebraska-Lincoln, Lincoln, NE, USA, and Prashanta Dutta, Washington State University, Pullman, WA, USA
Marja-Liisa Riekkola, Patricia Stege, Ai-Jun Wang, Lucia D’Ulivo, Joanna Witos , Geraldine Cilpa, Kati Vainikka, Katariina Öörnia, Petri T. Kovanena, University of Helsinki, Helsinki, FINLAND; aWihuri Research Institute, Helsinki, FINLAND
4:30 pm L-136 Genome-inspired DNA Aptamers for Nuclear Proteins. Linda B. McGown, Tian Zhang,
Brian Lin, Rensselaer Polytechnic Institute, Troy, NY, USA 4:55 pm L-137 Analysis of Human Biofluids using Chip-based Immunoaffinity Capillary Electrophoresis.
Terry M. Phillips, Heather Kalish, Edward Wellner, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, USA
4:30 pm L-140 Characterization of Inorganic Nanoparticles by Capillary Electrophoresis and Capillary
Isotachophoresis. Ute Pyell1, W. Bücking2, T. Nann2,3, 1University of Marburg, Marburg, GERMANY; 2Albert-Ludwig University of Freiburg, Freiburg, GERMANY; 3University of East Anglia (UEA), Norwich, UK
4:55 pm L-141 New Facts in Separation of Microorganisms using Electromigration Techniques.
Michał Szumski, Ewelina Dziubakiewicz, Ewa Kłodzińska, Katarzyna Hrynkiewicz, Jacek Szeliga, Marek Jackowski, Bogusław Buszewski, Nicolaus Copernicus University, Toruń, POLAND
5:20 pm L-142 Characterization of Quantum Dots Conjugates With Antibodies by Capillary
Electrophoresis. Ivona Voracova, Marcela Liskova, Karel Kleparnik, Vera Hezinova, Jan Prikryl, Frantisek Foret, Institute of Analytical Chemistry of the AS CR, Brno, CZECH REPUBLIC
5:45 pm Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 14 Monday, August 30, 2010 – Afternoon Parallel Sessions
SESSION 6C. INSTRUMENTATION AND DEVICES LOCATION: COMPOSITE ROOM (3rd Floor) Session Chairs: Cornelius F. Ivory, Washington State University, Pullman, WA, USA, and Gyoo Yeol Jung, POSTECH, Gyeongbuk, KOREA
4:05 pm L-143 Rapid Detection and Quantitation of Botulinum Neurotoxin/A Presence and Enzymatic
Activity using a Portable Diagnostic Platform. Chung-Yan Koh1, Gregory J. Sommer1, Bal Ram Singh2, Anup K. Singh1, Anson V. Hatch1, 1Sandia National Laboratories, Livermore, CA, USA; 2University of Massachusetts Dartmouth, North Dartmouth, MA, USA
4:30 pm L-144 Capillary Electrophoresis (CE) as a Promising Technology for Field and Point-of-care
Instruments. Mihkel Kaljurand, Merike Vaher, Tallinn University of Technology, Tallinn, ESTONIA
4:55 pm L-145 Lab-on-a-chip Device for Continuous Particle and Cell Separation Based on Electrical
Properties via AC-Dielectrophoresis. Barbaros Cetin1, Dongqing Li2, 1Middle East Technical University, Mersin, TURKEY; 2 University of Waterloo, Waterloo, CANADA
5:20 pm L-146 Integration of Microchips, Capillary Electrophoresis, and Electrochemical Detection with
Robotic Platforms to Perform Remote Chemical Analysis. Claudimir L. do Lago1, Carlos A. Neves1, Eric Tavares da Costa1, Guilherme Minoru Otta1, Marcelo Fagundes Barros1, Thiago Garcia1, Arturo A. Ayon2, Carlos D. Garcia2, 1University of Sao Paulo, Sao Paulo, BRAZIL; 2University of Texas at San Antonio, San Antonio, TX, USA
5:45 pm Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 15 Tuesday, August 31, 2010 – Plenary Lectures & Morning Parallel Sessions 7:30 am SYMPOSIUM REGISTRATION OPEN (2nd Floor) 9:00am-6:00pm EXPOSITION OPEN (5th Floor)
SESSION 7. PLENARY LECTURES LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chair: Fred Regnier, Perfinity Biosystems, West Lafayette, IN, USA
8:00 am L-201 Electromigration of Single Protein and DNA Molecules in Nanopores. Edward S. Yeung, Changbei Ma, Iowa State University, Ames, IA, USA [PLENARY LECTURE]
8:30 am L-202 Lectin-based Structural Glycomics: Development of Lectin Microarray and Its Diverse
Applications. Jun Hirabayashi, National Institute of Advanced Industrial Science and Technology, Tsukuba, JAPAN [PLENARY LECTURE]
9:00 am BREAK in Exhibit Hall (5th Floor)
SESSION 8A. MICROFLUIDICS 1 LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: Tae Seok Seo, KAIST, Daejeon, KOREA, and Victor M. Ugaz, Texas A&M University, College Station, TX, USA
9:30 am L-203 Acoustophoresis - A Generic Platform for Microfluidic Cell Handling and Sample
Processing, Thomas Laurell, Lund University, Lund, SWEDEN [KEYNOTE LECTURE] 10:00 am L-204 The Microfluidic ‘Pinwheel Effect’: Label-Free DNA Detection, Quantitation, Cell
Counting and More. J. Li1, D. Leslie1, D. Finkler1, S. Barker1, James P. Landers1,2, 1University of Virginia, 2University of Virginia Health Sciences Center, Charlottesville, VA, USA [KEYNOTE LECTURE]
10:25 am L-205 Microfluidic Glass Chips with Integrated Nanoelectrospray Emitters for Chip
Electrophoresis – MS Coupling. Detlev Belder, University of Leipzig, Leipzig, GERMANY 10:50 am L-206 Microscale Isoelectric Fractionation Membranes for Rapid Protein Purification and
Enrichment. Greg J. Sommer, Junyu Mai, Anup K. Singh, Anson V. Hatch, Sandia National Laboratories, Livermore, CA, USA
11:15 am Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 16 Tuesday, August 31, 2010 – Morning Parallel Sessions
SESSION 8B. BIOANALYSIS 1 LOCATION: DORIC ROOM (4th Floor) Session Chairs: Gerard Rozing, Agilent Technologies, Waldbronn, GERMANY, and Djuro Josic, Brown University, Providence, RI, USA
9:30 am L-207 Finding the Genes that Carry Risk for Common Disease: Population Requirements and
Strategy for use of Parallel Denaturing Capillary Electrophoresis Arrays. William G. Thilly1, S. Morgenthaler2, P. O. Ekstrom3, I. W.Hunter1, 1MIT, Cambridge, MA, USA; 2Ecole Polytechnique Federale, Lausanne, SWITZERLAND; 3Radium Hospital, Oslo, NORWAY [KEYNOTE LECTURE]
10:00 am L-208 Micro and Nanotechnology in Bioanalysis. Frantisek Foret, Institute of Analytical Chemistry of the ASCR, Brno, CZECH REPUBLIC
10:25 am L-209 Separating DNA and Protein Molecules in Gel-free Solutions using Bare
Nanocapillaries. Shaorong Liu, Xiayan Wang Joann J. Lu, The University of Oklahoma, Norman, OK, USA
10:50 am L-210 Analytical Assays Based on Capillary Electrophoresis for Biologically Interesting
Molecules Such as Taurine, Peptides and Heparine. Reinhard H. H. Neubert, Martin Luther University, Halle (Saale), GERMANY
11:15 am Pause
SESSION 8C. MINIATURIZATION IN THE WEST – MICROFLUIDICS 2 (session organized by Franta Foret) LOCATION: COMPOSITE ROOM (3rd Floor) Session Chair: Frantisek Foret, Institute of Analytical Chemistry of the ASCR, Brno, CZECH REPUBLIC
9:30 am L-211 Microfabricated Chemical Instrumentation: Systems for Acquiring Chemical and
Biochemical Information. J. Michael Ramsey, University of North Carolina, Chapel Hill, NC, USA [KEYNOTE LECTURE]
10:00 am L-212 Improved Optical Detection Setups for Microfluidic Devices. Thomas G. Jensen, Pelle Ohlsson,Jörg P. Kutter, Technical University of Denmark, Lyngby, DENMARK
10:25 am L-213 Novel Assay and Portable Instrument for Label-Free Toxin Detection. M. Bercovici1,
G.V. Kaigala1, S.S. Bahga1, C.J. Backhouse2, Juan G. Santiago1, 1Stanford University, Stanford, CA, USA; 2University of Alberta, Edmonton, CANADA
10:50 am L-214 Dielectrophoretic Manipulation of Particles Employing Low Frequency Alternating
Electric Fields in an Array of Insulators. Javier L. Baylon-Cardiel1, Nadia M. Jesús-Pérez1, Ana V. Chávez-Santoscoy1, Sergio O. Martínez-Chapa1, Blanca H. Lapizco-Encinas2, 1Tecnológico de Monterrey, Monterrey, MEXICO; 2CINVESTAV-Monterrey, Apodaca, MEXICO
11:15 am Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 17 Tuesday, August 31, 2010 – Morning Parallel Sessions
SESSION 9A. (BIO)PHARMACEUTICALS LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: Bezhan Chankvetadze, Tbilisi State University, Tbilisi, GEORGIA, and Shahab Shamsi, Georgia State University, Atlanta, GA, USA
11:20 am L-215 New Approaches for Profiling of (Bio)pharmaceuticals by CE-MS.
Gerhardus J. de Jong, Paul Hommerson, Rob Haselberg, Govert W. Somsen, Utrecht University, Utrecht, THE NETHERLANDS
11:50 am L-216 Multiplexed Capillary Electrophoresis with UV Detection to Support High Throughput
Pharmaceutical Research. Jolanta Plewa, Xiaoyi Gong, Margaret Figus, Dorothy Levorse, Lili Zhou, Christopher Welch, Merck & Co., Inc., Rahway, NJ, USA
12:15 pm L-217 A Multisite Study of Assay Performance for a Microfluidic CE-SDS Assay System.
Joseph Siemiatkoski, Biogen Idec, Cambridge, MA, USA
12:35 pm Pause – Lunch on own 12:45-1:45 pm Free Vendor Workshop – Must register to attend free workshop
Developments in Capillary Electrophoresis at Beckman Coulter LOCATION: TUSCAN ROOM (3rd Floor) You must sign up to attend the Free Vendor Workshop by contacting Beckman Coulter. A complimentary lunch will be provided during the workshop for those who sign up.
1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-300 and P-400 series of posters presented
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 18 Tuesday, August 31, 2010 – Morning Parallel Sessions
SESSION 9B. DETECTION DEVICES AND APPROACHES LOCATION: DORIC ROOM (4th Floor) Session Chairs: Ute Pyell, University of Marburg, Marburg, GERMANY, and Haleem Issaq, SAIC-Frederick, Inc., Frederick, MD, USA
11:20 am L-218 Aerosol Analysis by Microchip Electrophoresis. Scott D. Noblitt1, Susanne V. Hering3,
Jeffery L Collett, Jr.1, Charles S. Henry1, 1Colorado State University, Fort Collins, CO, USA; 3Aerosol Dynamics, Inc., Berkeley, CA, USA [KEYNOTE LECTURE]
11:50 am L-219 Electrochemical Detection on Capillary Electrophoresis Microchips: Reliable Analytical
Platforms for Analyte Sensing in Real Domains. Alberto Escarpa, University of Alcalá, Madrid, SPAIN
12:15 pm L-220 Combined Contactless Conductometric, Photometric, and Fluorimetric Single Point
Detector for Capillary Separation Methods. Markéta Ryvolová1,2, Mahbub Akhter3, Jan Preisler2, Pavel Krásenský2, František Foret4, Peter C. Hauser5, Pleun Maaskant3, Mirek Macka1,6, 1Dublin City University, Dublin, IRELAND; 2Masaryk University, Brno, CZECH REPUBLIC; 3Tyndall National Institute, Cork, IRELAND; 4Institute of Analytical Chemistry, Brno, CZECH REPUBLIC; 5University of Basel, SWITZERLAND; 6University of Tasmania, Hobart, AUSTRALIA
12:35 pm Pause – Lunch on own 12:45-1:45 pm Free Vendor Workshop – Must register to attend free workshop
Developments in Capillary Electrophoresis at Beckman Coulter LOCATION: TUSCAN ROOM (3rd Floor) You must sign up to attend the Free Vendor Workshop by contacting Beckman Coulter. A complimentary lunch will be provided during the workshop for those who sign up.
1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-300 and P-400 series of posters presented
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 19 Tuesday, August 31, 2010 – Morning Parallel Sessions
SESSION 9C. YOUNG SCIENTISTS 1 LOCATION: COMPOSITE ROOM (3rd Floor) Session Chairs: Marja-Liisa Riekkola, University of Helsinki, Helsinki, FINLAND, and Linda McGown, Rensselaer Polytechnic Institute, Troy, NY, USA
11:20 am L-221 Microchip Free-flow Electrophoresis. Stefan Köhler, C. Weilbeer, D. Belder, University
of Leipzig, Leipzig, GERMANY 11:35 am L-222 Drop-based IEF for Protein Separation. Ana Egatz-Gomez1, Wolfgang Thormann2,
1Texas A&M University, College Station, TX, USA; 2University of Bern, Bern, SWITZERLAND 11:50 am L-223 Regulation of DNA Translocation through a Nanopore by a Fluidic Field Effect
Transistor. Ye Ai, Shizhi Qian, Old Dominion University, Norfolk, VA, USA 12:05 pm L-224 Potential of Surface-Enhanced Raman Scattering Detector for Electrophoresis.
Jan Prikryl, Karel Klepárník, František Foret, Institute of Analytical Chemistry of the ASCR, Brno, CZECH REPUBLIC
12:20 pm L-225 A Method to Separate Dielectrophoresis and AC Electroosmosis. Jingyu Wang,
H. Daniel Ou-Yang, Lehigh University, Bethlehem, PA, USA 12:35 pm Pause – Lunch on own 12:45-1:45 pm Free Vendor Workshop – Must register to attend free workshop
Developments in Capillary Electrophoresis at Beckman Coulter LOCATION: TUSCAN ROOM (3rd Floor) You must sign up to attend the Free Vendor Workshop by contacting Beckman Coulter. A complimentary lunch will be provided during the workshop for those who sign up.
1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-300 and P-400 series of posters presented
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 20 Tuesday, August 31, 2010 – Afternoon Parallel Sessions
SESSION 10A. MINIATURIZATION IN ASIA PACIFIC – MICROFLUIDICS 3 (session organized by Bingcheng Lin) LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chair: Bingcheng Lin, Dalian Institute of Chemical Physics, Liaoning, CHINA
3:00 pm L-226 High Performance Separation and Detection of Biogenic Compounds by Microchip Electrophoresis Using On-line Sample Concentration Techniques. Takayuki Kawai, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka, Kyoto University, Kyoto, JAPAN
3:25 pm L-227 Application of Optofluidic Sensors for Highly Sensitive Immunoassay. Jaebum Choo,
Hanyang University, Ansan, KOREA 3:50 pm L-228 Integrated Microfluidic Systems for Fast Virus Detection. Gwo-Bin Lee, National Cheng
Kung University, Tainan, TAIWAN 4:10 pm L-229 Polyelectrolytic Gel Plugs on Chip and Their Applications. Taek Dong Chung,
Seoul National University, Seoul, KOREA 4:30 pm L-230 Novel Microfabrication and Surface Modification Methods for Polymer Microfluidic
Chips. W. Mike Arnold, Andrea Bubendorfer, Ingrid Hoek, Tim Kemmitt, Roderick Stanley, Industrial Research Limited and the MacDiarmid Institute for Advanced Materials and Nanotechnology. Lower Hutt, NEW ZEALAND
4:50 pm Pause
SESSION 10B. CE-MS LOCATION: DORIC ROOM (4th Floor) Session Chairs: William G. Thilly, MIT, Cambridge, MA, USA, and Edward Yeung, Iowa State University, Iowa, USA
3:00 pm L-231 Direct Coupling of Single Drop Microextraction with Capillary Electrophoresis-Mass
Spectrometry. Jihye Kim, Kihwan Choi, Doo Soo Chung, Seoul National University, Seoul, KOREA [KEYNOTE LECTURE]
3:30 pm L-232 Two Interfaces for Efficient Coupling of CE and MS: Iontophoretic Fraction Collection
for CE-MALDI-MS and Sheathless Interfacing of CE and ESI-MS through a Porous Tip. Jean-Marc Busnel, Beckman Coulter, Brea, CA, USA
3:50 pm L-233 Coupling CE to MS: The Plug and Play Approach vs. Customized Interface Design.
Christian W. Klampfl, Johannes Kepler-University Linz, Linz, AUSTRIA 4:10 pm L-234 The Role of MS Detection in Ligand Exchange Capillary Electrophoresis.
Vincenzo Cucinotta, A. M. Contino, S. Giuffrida, G. Maccarrone, M. Messina, University of Catania, Catania, ITALY
4:30 pm L-235 Optimizing the Union between Electrophoretic Mobility and Electrospray Ionization.
Jeff Chapman, Beckman Coulter, Fullerton, CA, USA 4:50 pm Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 21 Tuesday, August 31, 2010 – Afternoon Parallel Sessions
SESSION 10C. APPLICATIONS AND METHODOLOGIES 2 LOCATION: COMPOSITE ROOM (3rd Floor) Session Chairs: Terry Phillips, NIH, Bethesda, MD, USA and Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
3:00 pm L-236 Electromigration Acid-Base Titration via Moving Neutralization Boundary
Electrophoresis. Qing Yang§,†, Cheng-Xi Cao§, Shan-Sheng Huang†, Liu-Yin Fan§, §Shanghai Jiao Tong University, Shanghai, CHINA; †Shanghai Normal University, Shanghai, CHINA
3:25 pm L-237 Preparation of Monolithic Capillary Columns for Proteome Analysis. Hanfa Zou,
Mingliang Ye, Fangjun Wang, Minghuo Wu, Renan Wu, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, CHINA
3:50 pm L-238 New Insights on Solubilization Loci in SDS Micelles from QSRR and Molecular
Dynamics Experiments. Carlos A. Fuzo1, Luís G. Dias1, João P.S. Farah2, Marina F.M. Tavares2, 1University of Sao Paulo, Ribeirao Preto, BRAZIL; 2University of Sao Paulo, Sao Paulo, BRAZIL
4:10 pm L-239 Hydroxyethylcellulose-graft-poly (2-(dimethylamino)ethyl methacrylate) as Physically
Adsorbed Coating for Protein Separation by CE. Fuhu Cao1, Zhaofeng Luo2, Shuhua Peng1, Dan Zhou1, Yanmei Wang1, 1University of Science and Technology of China, Hefei, P. R. CHINA; 2University of Science and Technology of China, Hefei, P. R. CHINA
4:30 pm L-240 Analysis of Fibre Dyes by Capillary Electrophoresis – Quadrupole Time-of-Flight Mass
Spectrometry. Jason Lee1, Philip Doble1, Claude Roux1, Lucas Blanes1, Jane Hemmings2, 1University of Technology, Sydney, AUSTRALIA; 2Australian Federal Police, Canberra, AUSTRALIA
4:50 pm Pause
SESSION 11A. PROTEOMICS AND GLYCOPROTEOMICS LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: Jonas Bergquist, Uppsala University, Uppsala, SWEDEN, and Paolo Iadarola, Università di Pavia, ITALY
4:55 pm L-241 Off- and On-Line Fast and Efficient Release of Glycoprotein N-Glycans Prior to MS and
LC-MSMS. Yehia Mechref, Yazen R. Jmeian, Indiana University, Bloomington, IN, USA 5:20 pm L-242 Proteomics Workflows and the Challenges to Separate and Identify Thousands of
Proteins. Aran Paulus, Bio-Rad Laboratories, Hercules, CA, USA 5:45 pm L-243 Rapid Analysis of N-Glycans By Microchip-CE-LIF. M. Takahashi, R. Detloff, R. Singh,
Bahram Fathollahi, Caliper Life Sciences, Alameda, CA, USA (presented by Joseph Siemiatkoski, Biogen Idec)
6:10 pm Pause 7:30 pm Conference Banquet Dinner
Ticket Required. Misplaced or lost tickets will not be replaced.
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 22 Tuesday, August 31, 2010 – Afternoon Parallel Sessions
SESSION 11B. GENOTYPING AND DNA SEQUENCING AND SEPARATION LOCATION: DORIC ROOM (4th Floor) Session Chairs: Kevin D. Dorfman, University of Minnesota, Minneapolis, MN, USA, and James W. Schneider, Carnegie Mellon University, Pittsburgh, PA, USA
4:55 pm L-244 Free-solution DNA Sequencing in Micro- and Nano-electrophoresis Systems Enabled
by Protein Polymer Drag-tags. Annelise Barron, Stanford University, Stanford, CA, USA 5:20 pm L-245 An Examination of PCR Inhibition in Forensic DNA Genotyping by Real Time PCR and
Capillary Electrophoresis. Bruce McCord, Robyn Thompson, Kerry Opel, Ohio University, Athens, OH, USA
5:45 pm L-246 A Novel Method for Analyzing Primer Heterodimers using Free-solution Capillary
Electrophoresis to Probe Dimerization Risk. Samantha M. Desmarais, Thomas K. Leitner, Steven M. Wolinsky, Annelise E. Barron, Stanford University, Stanford, CA, USA
6:10 pm Pause 7:30 pm Conference Banquet Dinner
Ticket Required. Misplaced or lost tickets will not be replaced.
SESSION 11C. CAPILLARY ELECTROCHROMATOGRAPHY AND ELECTROKINETIC CHROMATOGRAPHY LOCATION: COMPOSITE ROOM (3rd Floor) Session Chairs: Adam T. Woolley, Brigham Young University, Provo, UT, USA, and Joe Foley, Drexel University, Philadelphia, PA, USA
4:55 pm L-247 Novel Neutral Monolithic Columns for Capillary Electrochromatography of a Wide
Range of Species. Ziad El Rassi, S. Karenga, Oklahoma State University, Stillwater, OK, USA 5:20 pm L-248 Pressurized Planar Electrochromatography as a High Performance Separation Mode
and an Effective Tool for Two-dimensional Separations. Tadeusz H. Dzido, Adam Chomicki, Paweł Płocharz, Aneta Hałka, Piotr Ślązak, Anna Klimek-Turek, Medical University of Lublin, Lublin, POLAND
5:45 pm L-249 Self-assembled Phospholipids: A Thermally Responsive Additive for Enhanced
Capillary Electrophoresis Separations. Lisa A. Holland, Stephanie Archer-Hartmann, Xingwei Wu, Ted Langan, C. Eugene Bennett, West Virginia University, Morgantown, WV, USA
6:10 pm Pause 7:30 pm Conference Banquet Dinner
Ticket Required. Misplaced or lost tickets will not be replaced.
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 23 Wednesday, September 1, 2010 – Plenary Lecture & Morning Parallel Sessions 8:00 am SYMPOSIUM REGISTRATION OPEN (2nd Floor)
SESSION 12. PLENARY LECTURE LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chair: Gerhardus J. de Jong, Utrecht University, Utrecht, THE NETHERLANDS
8:15 am L-301 Ionic Liquids in Separations and Mass Spectrometry, a New Frontier. Daniel W. Armstrong, University of Texas at Arlington, Arlington, TX, USA [PLENARY LECTURE]
8:45 am Pause
SESSION 13A. FUNDAMENTALS AND METHODOLOGIES 1 OCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: Vaclav Kasicka, Institute of Organic Chemistry and Biochemistry, Praha, CZECH REPUBLIC, and Detlev Belder, University of Leipzig, Leipzig, GERMANY
8:50 am L-302 System Peaks in Electrophoresis: Why Do They Appear and How to Avoid Them?
Bohuslav Gas, Charles University in Prague, Prague, CZECH REPUBLIC 9:15 am L-303 Investigation of the Stereodynamics of Molecules and Catalyzed Reactions by CE.
Oliver Trapp, Ruprecht-Karls-Universität Heidelberg, Heidelberg, GERMANY 9:40 am L-304 Gradient Elution Moving Boundary Electrophoresis: A Simple Technique for Analysis of
Complex Samples. David Ross*,†, Paul Flanigan, IV‡, Alyssa Henry§, Jason Kralj†, Catherine Lo†, Matthew Munson†, Eugenia Romantseva†,**, Jonathan Shackman‡, Elizabeth A. Strychalski†, †National Institute of Standards and Technology; ‡Temple University; §Applied Research Associates, Inc. *Current affiliation: National Institute of Biomedical Imaging and Engineering, Gaithersburg, MD, USA
10:05 am L-305 Stability Studies of Cysteine Functionalized CdSe Nanoparticles via Capillary Electrophoresis. Jared S. Baker, David F. Watson, Luis A. Colón, The State University of New York at Buffalo, Buffalo, NY, USA
10:30 am BREAK in Exhibit Hall (5th Floor)
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 24 Wednesday, September 1, 2010 – Morning Parallel Sessions
SESSION 13B. BIOANALYSIS 2 LOCATION: DORIC ROOM (4th Floor) Session Chairs: Yehia S. Mechref, Texas Tech University, Lubbock, TX, USA, and Alejandro Cifuentes, IFI, CSIC, Madrid, SPAIN
8:50 am L-306 Has Capillary Electrophoresis Matured into a Robust and Routine Bio-analytical
Measurement Method? Assessment and Examples. Gerard P. Rozing, Christian Wenz, Agilent Technologies, Waldbronn, GERMANY
9:15 am L-307 Recent Results in Forensic Analysis by Using Capillary Electrodriven Techniques.
Salvatore Fanali, Zeineb Aturki, Italian National Council of Research, Monterotondo Scalo, ITALY
9:40 am L-308 Analysis of Proteins by Capillary Sieving Electrophoresis with Postcolumn
10:05 am L-309 Biomarker Quantitation through Affinity Extraction Coupled with Capillary
Electrophoresis in a Microfabricated Device. Adam T. Woolley, Weichun Yang, Pamela Nge, Ming Yu, Brigham Young University, Provo, UT, USA
10:30 am BREAK in Exhibit Hall (5th Floor)
SESSION 13C. GENETIC ANALYSIS TECHNOLOGIES (session organized by Annelise Barron) LOCATION: COMPOSITE ROOM (3rd Floor) Session Chair: Annelise Barron, Stanford University, Stanford, CA, USA
8:50 am L-310 Capillary Electrophoretic Microdevice for High-Performance Short Tandem Repeat Genotyping. Tae Seok Seo, KAIST, Daejeon, REPUBLIC OF KOREA
9:15 am L-311 Selecting Gel Pore Morphologies to Exploit Entropic Trapping for Enhanced Microchip
Electrophoresis Separations. Nan Shi, Victor M. Ugaz, Artie McFerrin, Texas A&M University, College Station, TX, USA
9:40 am L-312 A Singular View of the Genome. David C. Schwartz, University of Wisconsin-Madison,
Madison WI, USA 10:05 am L-313 Post-Light Sequencing with Semiconductor Chips. John Leamon, Ion Torrent Systems,
Guilford, CT, USA 10:30 am BREAK in Exhibit Hall (5th Floor)
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 25 Wednesday, September 1, 2010 – Morning Parallel Sessions
SESSION 14A. SAMPLE PREPARATION AND CONCENTRATION LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: Ludmila Krivankova, Institute of Analytical Chemistry, Brno, CZECH REPUBLIC and Mihkel Kaljurand, Tallinn University of Technology, Tallinn, ESTONIA
10:50 am L-314 Development of an Amine-reactive, Hydrophilic Fluorophore having pH-independent
Fluorescence Properties and Mobilities in the 3 < pH < 10 Range. Gyula Vigh, Ming-Chien Li, Roy Estrada, Texas A&M University, College Station, TX, USA
11:15 am L-315 Off-line Coupled Mass Spectrometry with the Sample Pretreatment by the Column-
coupling Capillary Isotachophoresis. Jozef Marák, Andrea Stanová, S. Gajdoštínová, Dušan Kaniansky, Comenius University, Bratislava, SLOVAKIA
11:40 am L-316 Isotachophoresis on a Microchip with a 100x Reducing Union. Danny Bottenus,
Cornelius F. Ivory, Washington State University, Pullman, WA, USA 12:05 pm L-317 Principles and Research Applications for Modular Approach to Designing Microscale
Systems from Proof-of-Concept to Product. Yolanda Fintschenko, LabSmith, Inc., Livermore, CA, USA
12:30 pm Pause – Lunch on own 1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-500 and P-600 series of posters presented
SESSION 14B. PROTEOMICS 2 / METABOLOMICS LOCATION: DORIC ROOM (4th Floor) Session Chairs: Norberto A. Guzman, Princeton Biochemicals Inc., Princeton, NJ, USA, and Andrei R. Timerbaev, Vernadsky Institute of Geochemistry and Analytical Chemistry, Moscow, RUSSIA
10:50 am L-318 Biomarker Discovery: It’s Much More than a Mass Spec Problem.
Timothy D. Veenstra, NCI-Frederick, Frederick, MD, USA [KEYNOTE LECTURE] 11:20 am L-319 Proteomic Analysis of MCF7 Breast Cancer Cell Cycle. Milagros Perez, Iulia M. Lazar,
Virginia Polytechnic Institute and State University, Blacksburg, VA, USA 11:45 am L-320 Comprehensive Thiol Redox Status Determination using CE-MS for Metabolomics.
Lisa D'Agostino, Richard Lee, Philip Britz-McKibbin, McMaster University, Hamilton, CANADA 12:10 pm L-321 Les Maîtres de L’orge: How to Nail Down the Beer Proteome via Combinatorial Peptide
Ligand Libraries. Elisa Fasoli, Pier Giorgio Righetti, Politecnico di Milano, Milano, ITALY 12:30 pm Pause – Lunch on own 1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-500 and P-600 series of posters presented
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 26 Wednesday, September 1, 2010 – Morning Parallel Sessions
SESSION 14C. YOUNG SCIENTISTS 2 LOCATION: COMPOSITE ROOM (3rd Floor) Session Chairs: Alberto Escarpa, University of Alcalá, Alcalá de Henares, Madrid, SPAIN, and Lisa A. Holland, West Virginia University, Morgantown, WV, USA
10:50 am L-322 A Rapid Penta STR Screening Method by Microchip Capillary Electrophoresis.
Maurice J. Aboud1, Marcus Gassmann2, Bruce McCord1, 1Florida International University, Miami, FL, USA; 2Agilent Technologies, Waldbronn, GERMANY
11:05 am L-323 Comparison of Single-chiral Selector and Multi-chiral Selector Enantioseparation
Systems. Jana Svobodová, Pavel Dubský, Eva Tesarová, Bohuslav Gaš, Charles University, Prague, CZECH REPUBLIC
11:20 am L-324 Enhancing the Coverage of the Urinary Metabolome using Sheathless CE-ToF-MS.
Rawi Ramautar, Andre Deelder, Oleg A. Mayboroda, Leiden University Medical Center, Leiden, THE NETHERLANDS
11:35 am L-325 Capillary Zone Electrophoresis-Based Methods for Determining Metal Ion and Chelating
Agent Speciation, and Their use in Environmental Chemistry. Nathan E. Boland, Alan T. Stone, The Johns Hopkins University, Baltimore, MD, USA
11:50 am L-326 Isoelectric Trapping Pre-fractionation Coupled with LC-MS/MS for Quantitative
Proteomic Studies of Adipocyte Mitochondria. Stephanie M. Cologna, Billy W. Newton, William K. Russell, Arul Jayaraman, Gyula Vigh, David H. Russell, Texas A&M University, College Station, TX, USA
12:05 pm L-327 Flow in a Planar Electro-Chromatograph. René Laskowski1, H.-J. Bart1, C. Hofmann2,
G. Menges2, B. Werner2, P. Löb2, V. Hessel2, H. Gruber-Wölfler3, P. Feenstra3, M.-G. Braunbruck3, J. Khinast3, 1TU Kaiserslautern, Kaiserslautern, GERMANY; 2Institut für Mikrotechnik Mainz GmbH, Mainz, GERMANY; 3Graz University of Technology, Graz, AUSTRIA
12:20 pm L-328 Nanoscale Electrokinetic Transport of DNA in Pressure-Driven Flow.
Denitsa Milanova, Juan G. Santiago, Annelise E. Barron, Stanford University, Stanford, CA, USA
12:35 pm Pause – Lunch on own 1:50-2:55 pm POSTER SESSION in Exhibit Hall (5th Floor)
P-500 and P-600 series of posters presented
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 27
Wednesday, September 1, 2010 – Afternoon Parallel Sessions
SESSION 15A. MULTIDISCIPLINARY KEYNOTE LECTURES LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chairs: Jörg P. Kutter, Technical University of Denmark, Lyngby, DENMARK, and Christian W. Klampfl, Johannes Kepler-University Linz, Linz, AUSTRIA
3:00 pm L-329 Unraveling Cell to Cell Signaling in the Brain with Electrophoretic Separations and Mass Spectrometry. Jonathan V. Sweedler, University of Illinois, Urbana, IL, USA
3:25 pm L-330 Use of High-throughput Chromatography and Proteomics Technology for the
Optimization of Production and Quality Control of Therapeutic Proteins. Djuro Josic, COBRE Center for Cancer Research Development and Brown University, Providence, RI, USA
3:50 pm L-331 In vivo Chemical Sensing of Neurotransmitters using High-speed Chip Electrophoresis.
Robert T. Kennedy, University of Michigan, Ann Arbor, MI, USA 4:15 pm L-332 Polymer-based Microchips for the High Resolution Electrophoretic Separation of
Nucleic Acids and Proteins: Successes and Failures, Steven A. Soper, Louisiana State University, Baton Rouge, LA, USA
4:40 pm Pause
SESSION 15B. DNA SEPARATION AND SEQUENCING LOCATION: DORIC ROOM (4th Floor) Session Chairs: David C. Schwartz, University of Wisconsin-Madison, Madison WI, USA, and Shaorong Liu, The University of Oklahoma, Norman, OK, USA
3:00 pm L-333 Micelle-ELFSE Methods for Rapid Electrophoretic Separations of DNA.
James W. Schneider, Stephen Istivan, Angela Holmen, Max Fahrenkopf, Carnegie Mellon University, Pittsburgh PA, USA
3:25 pm L-334 DNA Electrophoresis in Microfabricated Post Arrays. Kevin D. Dorfman, Jia Ou,
Jaeseol Cho, Daniel W. Olson, Samuel J. Carpenter, University of Minnesota, Minneapolis, MN, USA
3:50 pm L-335 Sensitive and Multiplex Genetic Analysis System using CE-based SSCP Combined with
MLPA. Gyoo Yeol Jung, Gi Won Shin, Hee Sung Hwang, Boram Chung, POSTECH, Gyeongbuk, KOREA
4:15 pm L-336 High-fidelity Fraction Collection of Single-strand DNA Fragments Differing in Size by
Only One Base on a Microchip. Kai Sun1, Zheyu Li1, Misato Sunayama2, Ryoko Araki2, Kosei Ueno1, Masumi Abe2, Hiroaki Misawa1, 1Hokkaido University, Sapporo, JAPAN; 2National Institute of Radiological Sciences, Chiba, JAPAN
4:40 pm Pause
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 28 Wednesday, September 1, 2010 – Afternoon Parallel Sessions
SESSION 15C. FUNDAMENTALS AND METHODOLOGIES 2 LOCATION: COMPOSITE ROOM (3rd Floor) Session Chairs: Bohuslav Gas, Charles University in Prague, Prague 2, CZECH REPUBLIC, and Jesper Østergaard, University of Copenhagen, Copenhagen, DENMARK
3:00 pm L-337 Elastic Matching Method for Determination of Quasi Steady State in pH Dependent ITP.
Jaesool Shim1, Migyung Cho2, Prashanta Dutta3, 1Yeungnam University, Gyeongsan, KOREA; 2Tongmyong University, Busan, KOREA; 3Washington State University, Pullman, WA, USA
3:25 pm L-338 Resolution Equation for Electrokinetic Chromatography Based on Retention Factors
and Mobilities. Joe P. Foley, Drexel University, Philadelphia, PA, USA 3:50 pm L-339 Microfluidic Technologies for Large-Scale Proteomic Investigations – A Perspective on
Benefits and Challenges. Jarod L. Kabulski, Iulia M. Lazar, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
4:15 pm L-340 Separation of Short DNA in Nanoslit Arrays. Elizabeth A. Strychalski1*, Henry W. Lau1,
Lynden A. Archer1, 1Cornell University, Ithaca NY, USA; *Current Affiliation: National Institute of Standards and Technology, Gaithersburg, MD, USA
4:40 pm Pause
SESSION 16. CLOSING PLENARY LECTURE LOCATION: CORINTHIAN ROOM (2nd Floor) Session Chair: Steven A. Soper, Louisiana State University, Baton Rouge, LA, USA
4:55-5:25 pm L-341 Mass Linked Immunoselective Analysis. Fred Regnier, Kevin Meyer, Nicholas Herold, Perfinity Biosystems, West Lafayette, IN, USA [PLENARY LECTURE]
5:25 pm CLOSING REMARKS
LOCATION: CORINTHIAN ROOM (2nd Floor)
Closing Remarks by the Chair of ITP 2010. Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
Invitation by the Chair of ITP 2011. Bezhan Chankvetadze, Tbilisi State University, Tbilisi, GEORGIA
5:50 pm Meeting Adjourn
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 29
Poster Session 1. Sample Preparation, Concentration and Detection
Monday, August 30, 2010 (1:50-2:55 pm) All posters to be put onto poster boards on Monday morning
P-101 Sensitivity Enhancement for Capillary Zone Electrophoresis using a Novel Hybrid Sample
Injection Mode. Keiichi Fukushi1, Y. Yakushiji1, T. Hirokawa2, 1Kobe University, Kobe, JAPAN; 2Hiroshima University, Higashi-hiroshima, JAPAN
P-102 Potential of Surface-enhanced Raman Scattering Detector for Capillary Electrophoresis. Jan Prikryl,
Karel Klepárník, František Foret, Institute of Analytical Chemistry of the ASCR, Brno, CZECH REPUBLIC
P-103 Combination of Preparative Capillary Isotachophoresis and Mass Spectrometry in Analysis of High
Molecular Weight Compounds Present in Complex Matrices. Andrea Stanová, Jozef Marák, Monika Kondeková, Dušan Kaniansky, Comenius University, Bratislava, SLOVAK REPUBLIC
P-104 Numerical Computation and Experiments on Millions Fold Stacking of Peptides by a Derivative Moving
Reaction Boundary Initially Created by Formic Buffer and Strong Alkali in Electrophoresis. Jie Jina, Shan Lia,b, Wei Yana, Cheng-Xi Caoa, Wei Zhanga, Liu-Yin Fana, aShanghai Jiao Tong University, Shanghai, CHINA; bSouth China University of Technology, Guangzhou, CHINA
P-105 PDA/LIF-CE as an Indispensible Analytical Tool in the Development of a Hydrophilic Amine-reactive
Fluorophor. Ming-Chien Li, Roy Estrada, Gyula Vigh, Texas A&M University, College Station, TX, USA
P-106 A Fully Automated Capillary Electrophoresis Equipment for the Column-Coupling Technology as Joining Zone Electrophoresis with Isotachophoresis. Miroslava Halašiová, Róbert Bodor, Dušan Kaniansky Comenius University, Bratislava, SLOVAKIA
P-107 Sensitivity Enhancement for the Analysis of Amino Acids and Peptides by In-line Single Drop
Microextraction. Yang-ki Park, Kihwan Choi, Jihye Kim, Doo Soo Chung, Seoul National University, Seoul, KOREA
P-108 Selective Sweeping of Overloaded Zn(II) and Transient Isotachophoresis of Trace Metals in
Highly Saline Samples. Kihwan Choi, Jihye Kim, Doo Soo Chung, Seoul National University, Seoul, KOREA
P-109 Analysis of Fibre Dyes by Capillary Electrophoresis – Quadrupole Time-of-Flight Mass Spectrometry.
Jason Lee1, Philip Doble1, Claude Roux1, Lucas Blanes1, Jane Hemmings2, 1University of Technology, Sydney, AUSTRALIA, 2Australian Federal Police, Canberra, AUSTRALIA
P-110 Separation and Detection of Reactive Nitrogen Species from RAW 264.7 Cells using Microchip
Electrophoresis with Amperometric and Laser Induced Fluorescence Detection. Dulan B. Gunasekara1, Matthew K. Hulvey1, Derek T. Jensen1, Emilie R. Mainz1, Giuseppe Caruso1, Susan M. Lunte1,2, Christopher T. Culbertson1, 1University of Kansas, Lawrence, KS, USA; 2Kansas State University, Manhattan, KS, USA
P-111 In-Capillary Nanoreactor Based on Transverse Diffusion of Laminar Flow Profiles for Cytochrome P450
2C9 Isoform Reaction with Diclofenac. Roman Řemínek, Aleš Mádr, Zdeněk Glatz, Masaryk University, Brno, CZECH REPUBLIC
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 30
Poster Session 2. Food and Beverage Monday, August 30, 2010 (1:50-2:55 pm)
All posters to be put onto poster boards on Monday morning P-201 Evaluation of the Free Radical Scavenging Capability of Wheat Extracts by Capillary Electrophoresis.
Kati Helmja, M. Vaher, M. Kaljurand, Tallinn University of Technology, Tallinn, ESTONIA P-202 Electrophoretic Analysis of Toxic Heavy Metals in Water Resources with Contactless Conductivity
Detection. Sam F. Y. Li, H. F. Lau, N. M. Quek, National University of Singapore, Kent Ridge, SINGAPORE
P-203 Column-coupling Electrophoresis Chip Technology Operating Sequentially in Determining both the
Inorganic Cations and Anions in Water. Marián Masár, Pavol Kruk, Milan Luc, Dušan Kaniansky, Comenius University, Bratislava, SLOVAKIA
P-204 Determination of GHB (Good Night Cinderella) by Chromatography and Capillary Electrophoresis:
Review of Methods of Analysis of GHB and Its Analogues. Elizabete Campos de Lima, Diogo Lima da Silva, Universidade Federal do ABC, Santo André, BRAZIL
P-205 Determination of Acrylamide in Food by Capillary Electrophoresis with Quantum Dot-mediated LIF
Detection. Qidan Chen, Yingsing Fung, The University of Hong Kong, Hong Kong SAR, CHINA P-206 Determination of Phytic Acid and Inositolphosphates in Barley. František Kvasnička, Jana Čopíková,
Rudolf Ševčík, Jana Krátká, Andrej Syntytsya, Michal Voldřich, Institute of Chemical Technology, Prague, CZECH REPUBLIC
P-207 Analysis of Free Amino Acids in Honey. Vaclav Stanek, Hana Weiserova, Petr Cesla, Jan Fischer,
Pavel Jandera, University of Pardubice, Pardubice, CZECH REPUBLIC P-208 New Capillary Electrophoresis Method for Enrichment Study of Virgin Olive Oil with Antioxidants
Phenolic Acid Came from Natural Herbs. J. J. Berzas Nevado, V. Rodriguez Robledo, C. Sánchez-Carnerero Callado, University of Castilla-La Mancha, Ciudad Real, SPAIN
P-209 A Rapid and Sensitive Genetic Identification Method for Detecting Beer-Spoilage Bacteria and Wild
Yeast. Yong Wu, Manami Saha, Jean-Marc Busnel, BeeNa Lee, Lily Nan, Handy Yowanto, Jeff Chapman, Beckman Coulter Inc., Brea, CA, USA
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 31
Poster Session 3. Bioanalysis Tuesday, August 31, 2010 (1:50-2:55 pm)
All posters to be put onto poster boards on Monday morning P-301 Quantification of Branched-chain Amino Acids of Ascites by Capillary Electrophoresis with Light-emitted
P-302 Rapid Detection of Biogenic Amines from Human Intracutaneous Microdialysate Samples on Microchip
Electrophoresis Platforms. E. Guihena, D. Brennanb, M. Ludwigc, Detlev Belderc, W. L. Hod, B. Ramsayd, M. L. O’Connelle, W. T. O’Connora, aUniversity of Limerick, IRELAND; bUniversity College Cork, IRELAND; cLeipzig University, Leipzig, GERMANY; dMid Western Regional Hospital, Limerick, IRELAND; eUniversity of Limerick, IRELAND
P-303 Comprehensive Study on the Bio-safety of Single-walled Carbon Nanotubes. Ruibin Li, Ren’an Wu,
Fanjun Wang, Hongwei Liu, Hanfa Zou, Ling Yang, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, CHINA
P-304 MEKC Profiles of Human HDL Phospholipids. Chin-Pong Chong, Mine-Yine Liu, National Changhua
University of Education, Changhua, TAIWAN
P-305 Enhancing the Coverage of the Urinary Metabolome using Sheathless CE-ToF-MS. Rawi Ramautar, Andre Deelder, Oleg A. Mayboroda, Leiden University Medical Center, Leiden, THE NETHERLANDS
P-306 A New RP-RPLC Approach for Large-scale Phosphorylation Analysis of Human Liver. Chunxia Song,
Guanghui Han, Xinning Jiang, Zhiyuan Yu, Fangjun Wang, Rui Chen, Mingliang Ye, Hanfa Zou, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, P. R. CHINA
P-307 The Monitoring of Glycerol in the Microdialyzate of Adipose Tissues by CE with Contactless
Conductivity Detection. Petr Tuma, Eva Samcová, Klára Málková, Charles University, Prague, CZECH REPUBLIC
P-308 Optimization of Background Electrolyte Conditions for Determination Neutral Carbohydrates in Different
Natural Sources by Capillary Electrophoresis. Merike Vaher, Jana Kazarjan, Tallinn University of Technology, Tallinn, ESTONIA
P-309 A Rapid Penta STR Screening Method by Microchip Capillary Electrophoresis. Maurice J. Aboud1,
Marcus Gassmann2, Bruce McCord1, 1Florida International University, Miami, FL, USA; 2Agilent Technologies, Waldbronn, GERMANY
P-310 Isoelectric Trapping Pre-fractionation Coupled with LC-MS/MS for Quantitative Proteomic Studies of
Adipocyte Mitochondria. Stephanie M. Cologna, Billy W. Newton, William K. Russell, Arul Jayaraman, Gyula Vigh, David H. Russell, Texas A&M University, College Station, TX, USA
P-311 Reduction of Protein Concentration Range Differences Followed by Multicolumn Fractionation Prior to 2-
DE LC-MS/MS Profiling of Serum Proteins. Subhashini Selvaraju, Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
P-312 Comparability Study of Beckman PA800 Plus vs. Agilent 7100 CE for Running Capillary Sodium
Dodecyl Sulfate Electrophoresis. Junge Zhang, Simei Shan, Chaomei Lin, Johnson and Johnson Pharmaceutical Research and Development, Malvern, PA, USA
Derived from Human Somatic Cells. Kathryn Sciabica, Akitsu Hotta, Knut Woltjen, Handy Yowanto, Jeff Chapman, Beckman Coulter Inc., Brea, CA, USA
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 32 P-314 Quantitative Shot-Gun Proteomics for Revealing Gender Differences in Enzyme Contents for Rat Liver
Microsome. Huang-Jen Huang, Shu-Hui Chen, National Cheng Kung University, Tanan, TAIWAN P-315 Separation of Fucosylated and Non-fucosylated Carbohydrated Associated with Monoclonal Antibodies
using Capillary Electrophoresis. Sushma Rampal, Mark Lies, Hans Dewald, Beckman Coulter, Brea, CA, USA
P-316 A Novel Strategy for the Simultaneous Detection of Alternatively Spliced Transcripts: A Study of MEF2C
Exon Covariation. Kathryn Sciabica, Tod Gulick, Handy Yowanto, Jeff Chapman, Benne Parten, Beckman Coulter Inc., Brea, CA, USA
P-317 Application of Sheathless CE-MS for the Analysis of Phospho-Peptides. Bettina Sarg1, L. Kremser1, A.
Sobieszek2, H. Lindner1, 1Innsbruck Medical University, AUSTRIA; 2Austrian Academy of Sciences, AUSTRIA
P-318 Analytical Techniques for Investigating the Metabolism and Blood Brain Barrier Transport of the
Neuropeptide Dynorphin A 1-17. Giuseppe Caruso1,2, 3, Courtney D. Kuhnline1,3, Jane V. Aldrich1, Terry M. Philips5, Susan M. Lunte1,3, 1The University of Kansas, Lawrence, KS, USA; 2The University of Catania, Catania, ITALY; 3The Ralph N. Adams Institute for Bioanalytical Chemistry, Lawrence, KS, USA; 5The National Institute of Health, NIBIB, USA
P-319 Simple and Rapid CE-UV Method for the Assessment of Trail Pheromone Compounds of Leaf-cutting
Ants Venom Glands. Amanda Araujo Leitão1; Paulo Cesar Muniz de Lacerda Miranda1; Ana Valéria Colnaghi Simionato1,2, 1University of Campinas, São Paulo, BRAZIL; 2National Institute of Science and Technology in Bioanalytics
Poster Session 4. Forensic, Pharmaceutical and Clinical Analysis Tuesday, August 31, 2010 (1:50-2:55 pm)
All posters to be put onto poster boards on Monday morning P-401 Direct MEKC Analysis of Metallodrugs: May Micelle Focusing Be a Successful Approach? Lidia S.
Foteeva1, A.R. Timerbaev1, T. Hirokawa2, 1Vernadsky Institute of Geochemistry and Analytical Chemistry, Moscow, RUSSIA; 2Hiroshima University, Higashi-hiroshima, JAPAN
P-402 Rapid Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with
Contactless Conductivity Detection. Eva Samcová, Petr Tuma, Charles University, Prague, CZECH REPUBLIC
P-403 Assessment of Chiral Purity of Acyclic Nucleoside Phosphonates-based Anti-AIDS Drugs by Capillary
Electrophoresis. Petra Sazelova, Veronika Solinova, Vaclav Kasicka, Antonin Holy, Institute of Organic Chemistry and Biochemistry ASCR, Prague, CZECH REPUBLIC
P-404 Extraction of Lapachol from Tabebuia avellanedae with Supercritical Carbon Dioxide followed by
Capillary Electrophoresis with UV Detection. José Alberto Fracassi da Silva, Paulo de Tarso Vieira e Rosa, Fernanda Pereira Barbosa, Alexandre Zatkovskis Carvalho, Aline Ferreira Mora, State University of Campinas, São Paulo, BRAZIL
P-405 Determination of Sodium Alginate by Capillary Zone Electrophoresis with Capacitively Coupled
Contactless Conductivity Detection. José Alberto Fracassi da Silva, Alexandre Zatkovskis Carvalho, Letícia Souza Leite Vieira, State University of Campinas, São Paulo, BRAZIL
P-406 Analytical Instrument Qualification in Capillary Electrophoresis Using Infrared Thermometry. Claudia
Cianciulli, Hermann Wätzig, TU Braunschweig, Braunschweig, GERMANY
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 33 P-407 Study of Degradation Processes and Characterization of Omeprazole and Its Main Degradation
Products by Capillary Electrophoresis using Diode-array and Electrospray Ionization-Mass Spectrometry Detectors. J. J. Berzas Nevado, G. Castañeda Peñalvo, R. M. Rodríguez Dorado, V. Rodriguez Robledo, University of Castilla-La Mancha, Ciudad Real, SPAIN
P-408 Analysis of Amphetamine Analogues using the Portable Bioanalyzer 2100 Lab-on-a-Chip. Aimee Lloyd,
Lucas Blanes, Alison Beavis, Philip Doble, Claude Roux, University of Technology, Sydney (UTS), AUSTRALIA
Poster Session 5. Capillary Electrochromatography (CEC) and Electrokinetic Chromatography (EKC)
Wednesday, September 1, 2010 (1:50-2:55 pm) All posters to be put onto poster boards on Monday morning
P-501 The use of Novel Ionic liquid-in-water Microemulsion without the Addition of Organic Solvents in a
Capillary Electrophoretic System. Jun Cao, Haibin Qu, Yiyu Cheng, Zhejiang University, Hangzhou, CHINA
P-502 Flow in a Planar Electro-Chromatograph. René Laskowski1, H.-J. Bart1, C. Hofmann2, G. Menges2, B.
Werner2, P. Löb2, V. Hessel2, H. Gruber-Wölfler3, P. Feenstra3, M.-G. Braunbruck3, J. Khinast3, 1TU Kaiserslautern, Kaiserslautern, GERMANY; 2Institut für Mikrotechnik Mainz GmbH, Mainz, GERMANY; 3Graz University of Technology, Graz, AUSTRIA
P-503 Continuous Full Filling Capillary Electrochromatography - Evaluation of a New Separation Technique
using Nanoparticles and Electrospray Mass Spectrometry. David Malmströma, Jakob Axéna, Jonas Bergquista, Peter Vibergb, Peter Spégelb,c, Uppsala University, Uppsala, SWEDEN; bNanosep AB, Lund, SWEDEN; cLund University, Malmö University Hospital, Malmö, SWEDEN
P-504 Study of Different Membrane Models by Capillary Electrochromatography. Kert Martma, Xochitl
P-505 Enantioseparation of Anionic Compounds using Eremomycin as Chiral Selector by Different Variants of
Capillary Electrophoresis. Aleksandra F. Prokhorova, M.V. Lebedeva, E.N. Shapovalova, S.M. Staroverov*, O.A. Shpigun, Lomonosov Moscow State University, Moscow, RUSSIA; *JSC BioChemMack S&T, Moscow, RUSSIA
P-506 Comparison of Single-chiral Selector and Multi-chiral Selector Enantioseparation Systems. Jana
Svobodová, Pavel Dubský, Eva Tesarová, Bohuslav Gaš, Charles University, Prague, CZECH REPUBLIC
P-507 Extraction of Xenoestrogens from Water and Tissue using Molecularly Imprinted Polymers. Renata
Gadzała-Kopciuch, Michał Szumski, Bogusław Buszewski, Nicolaus Copernicus University, Toruń, POLAND
P-508 Hydroxylated, Neutral Octadecyl Acrylate Monolith With Fast Electroosmotic Flow and its Applications to
the Separation of Various Solutes Including Peptides and Proteins in the Absence of Electrostatic Interactions. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
P-509 Neutral Naphthyl Methacrylate-based Monolithic Columns for RP-CEC via Hydrophobic and π
Interactions. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
P-510 Mixed Ligand Monolithic Columns for Reversed-Phase Capillary Electrochromatography via Hydrophobic and π-Interactions. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 34 P-511 Controlling Retention, Selectivity and Magnitude of EOF by Segmented Monolithic Columns Consisting
of Octadecyl and Naphthyl Monolithic Segments. Applications to RP-CEC of Both Neutral and Charged Solutes. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
P-512 Naphthyl Methacrylate-phenylene Diacrylate Based Monolith (NPM) for RP-CEC of Both Neutral and
Charged Solutes via Hydrophobic and π-interactions. Samuel Karenga, Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
P-513 Novel Organic Polymer Monolith with Polar Ligands for Normal Phase Capillary
Electrochromatography/Chromatography of Polar Species and for Immuno-capturing of Specific Serum Proteins. Dilani Gunasena, Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
P-514 Methacrylate-based Monoliths at Varying Alkyl Chain Length for CEC of Proteins and Peptides–
Preparation, Chromatographic Characterization and Performance. Chanida Puangpila, Ziad El Rassi, Oklahoma State University, Stillwater, OK, USA
Poster Session 6. Fundamentals and Methodologies Wednesday, September 1, 2010 (1:50-2:55 pm)
All posters to be put onto poster boards on Monday morning
P-601 Regulation of DNA Translocation through a Nanopore by a Fluidic Field Effect Transistor. Ye Ai, Shizhi Qian, Old Dominion University, Norfolk, VA, USA
P-602 Microchip Free-flow Electrophoresis. Stefan Köhler, C. Weilbeer, D. Belder, University of Leipzig,
Leipzig, GERMANY P-603 Evaluation of the Precision of Dual-opposite-injection Capillary Electrophoresis. Donna M. Blackney,
Joe P. Foley, Drexel University, Philadelphia, PA, USA
P-604 Enhancing Resolutions of the Analytes in Capillary Zone Electrophoresis as Preceded by Capillary Isotachophoresis with Discrete Spacers. Róbert Bodor, Vladimíra Jánošová, Dušan Kaniansky, Comenius University, Bratislava, SLOVAKIA
P-605 Capillary Zone Electrophoresis-Based Methods for Determining Metal Ion and Chelating Agent
Speciation, and Their use in Environmental Chemistry. Nathan E. Boland, Alan T. Stone, The Johns Hopkins University, Baltimore, MD, USA
P-606 Electropherogram Processing Algorithms for Automatic Baseline Correction, Signal Alignment with the
Aid of Thermal Marks and Peak Identification for Portable Capillary Electrophoresis Instruments. Andrus Seiman, Merike Vaher, Mihkel Kaljurand, Tallinn University of Technology, Tallinn, ESTONIA
P-607 Proper Capillary Electrophoresis/Frontal Analysis (CE/FA) Assay for the Determination of Binding
Constants. Karine Vuignier, Jean-Luc Veuthey, Pierre-Alain Carrupt, Sophie Martel, Julie Schappler, University of Geneva, University of Lausanne, Geneva, SWITZERLAND
P-608 A Method to Separate Dielectrophoresis and AC Electroosmosis. Jingyu Wang, H. Daniel Ou-Yang,
Lehigh University, Bethlehem PA, USA
P-609 Drop-based IEF for Protein Separation. Ana Egatz-Gomez1, Wolfgang Thormann2, 1Texas A&M University, College Station, TX, USA; 2University of Bern, Bern, SWITZERLAND
P-610 Nanoscale Electrokinetic Transport of DNA in Pressure-Driven Flow. Denitsa Milanova, Juan G.
Santiago, Annelise E. Barron, Stanford University, Stanford, CA, USA
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 35 P-611 DNA-Modified Sieving Polymers for Metagenomic Profiling in Capillary Electrophoresis. Yingying Dong,
Linda B. McGown, Rensselaer Polytechnic Institute, Troy, NY, USA
P-612 Theoretical Models of Separation Selectivity for Charged Compounds in Micellar Electrokinetic Chromatography. Chanida Puangpila, Amorn Petsom, Thumnoon Nhujak, Chulalongkorn University, Bangkok, THAILAND
P-613 Multi-patterning of Biological Samples on Glass Substrate and Inside the Fused Microchannel by Using
Photochemical Reaction. Kihoon Jang, Yan Xu, Yo Tanaka, Takehiko Kitamori, The University of Tokyo, Tokyo, JAPAN
P-614 Development of Antibody Patterning Method in Extended-Nano Channels. Hisashi Shimizu, Kazuma
Mawatari, Takehiko Kitamori, The University of Tokyo, Tokyo,JAPAN
P-615 Development of Pressure-Driven Liquid Chromatography in Extended-Nanochannels; Automated Injection and Normal Phase Mode. Ryo Ishibashi, Kazuma Mawatari, Takehiko Kitamori, The University of Tokyo, Tokyo,JAPAN
P-616 Evaluation of Permanent Capillary Coatings for CIEF. Dušan Koval1 ,Hervé Cottet2, Václav Kašička1,
1Ústav Organické Chemie a Biochemie AV ČR, Prague, CZECH REPUBLIC; 2Université de Montpellier, Montpellier, FRANCE
P-617 Stability Studies of Cysteine Functionalized CdSe Nanoparticles via Capillary Electrophoresis. Jared S.
Baker, David F. Watson, Luis A. Colón, The State University of New York at Buffalo, Buffalo, NY, USA
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 36
List of Exhibitors AGILENT TECHNOLOGIES 2850 Centerville Road Wilmington, DE 19808, USA 800-227-9770 www.agilent.com/chem Agilent Technologies, a leading supplier of life science and chemical analysis instrumentation and services, provides solutions to pharmaceutical, biopharmaceutical, environmental, foods & flavors, and hydrocarbon industries, making possible the identification, quantification and analysis of atomic, molecular, physical and biological applications. Our comprehensive portfolio of instrumentation and solutions include Capillary Electrophoresis (CE), OffGel Electrophoresis (OGE) and CE-Mass Spec for the faster and more sensitive analysis of complex chemical and pharma samples. BECKMAN COULTER 250 S. Kraemer Blvd., Brea, CA 92821, USA 800-742-2345 www.CELeader.com Beckman Coulter provides solutions for the separation, identification, quantitation and characterization of many analytes, including ions, nucleic acids, basic drugs, enantiomers, proteins and carbohydrates. Our development initiative encompasses hardware, software and chemistry, with a long list of advancements including our NEW PA 800 plus Pharmaceutical Analysis System, sheathless CE-MS, and GeXP Genetic Analysis System for highly sensitive multiplex gene expression. CALIPER LIFE SCIENCES 68 Elm Street, Hopkinton, MA 01748, USA 508-435-9500 www.caliperls.com LabChip GX and GXII Microfluidic Systems®. Caliper’s LabChip GX and GXII provide high throughput bioanalysis of nucleic acids and proteins. Caliper’s proprietary microfluidic sipper chips enable sampling directly from either 96- or 384-well microplates. For processes from research through product QC. Key protein applications: Titre, expression optimization, purity, glycan screening. GxP Security Software® provides support for validated environments. EDAQ INC. 2205 Executive Circle, Colorado Springs, CO 80906, USA 719-576-7000 www.edaq.com eDAQ supplies analysis systems for CE and ICE applications. Capacitively-Coupled Contactless Conductivity Detection (C4D) systems are available that act as a CE controller/data recorder, or a simple analog detector that integrates with existing CE, IC, HPLC, systems. The detectors work with 150µ, 365µ CE tubing as well as 1/32, 1/16, 1/8 inch OD tubing or self made chips with the micro-fluidic platform. Also, newly released is the Microchip Capillary Electrophoresis System (MCE) which includes data analysis, control, sequencer, and microchips with on chip C4D electrodes.
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 37
List of Exhibitors LABSMITH, INC. 4659 Las Positas, Suite C, Livermore, CA 94550, USA 925-292-5161 www.labsmith.com LabSmith, Inc. develops and builds laboratory tools that further the art of research. LabSmith products control the often exasperating aspects of experimentation, including timing, synchronization, high voltage and current sourcing, fluid routing and event capture. We craft rugged, innovative, and affordable solutions to everyday lab chores, so that you can focus on science. Scientists use LabSmith equipment for everything from on-chip electrochromatography, sample preparation to imaging. WILEY-BLACKWELL PUBLISHING Wiley VCH Verlag GmbH Bochstrasse 12 D-69469 Weinheim, GERMANY www.wiley.com Wiley-Blackwell is the world's leading society publisher, publishing more than 1400 scholarly peer-reviewed journals including a vast selection of cell and molecular biology publications and a prestigious books collection, written and edited by the best scholars and instructors. Visit our exhibit to browse our extensive range of books and journals and collect a sample copy of our flagship separations journal ELECTROPHORESIS.
We thank the following organizations for their generous support of the program
I T P 2 0 1 0 F i n a l P r o g r a m P a g e | 38
NOTES
Tremont Grand Floor Plan of Meeting Space and Directions from the Tremont Hotel to the Tremont Grand
CORINTHIAN ROOM, 2nd FLOOR Take Hotel Elevator to the Fifth Floor Walk down Hallway (Skywalk) **You are now on the 2nd floor of the Tremont Grand** Walk through the Roman Strada Room Make the Forced Left to Find Corinthian Room 3rd, 4th, and 5th FLOORS OF THE TREMONT GRAND Take Hotel Elevator to the 5th Floor Walk down Hallway (Skywalk) **You are now on the 2nd Floor of the Tremont Grand** Take Silver Elevators on the left to the 3rd, 4th, and 5th Floors
