KOREA_U_SEOUL

Pear l -co l i : Escherichia coli converting CO2

into Pearl powder

Team

2013 iGEM KOREA_U_SEOUL

2009Integrated Heavy Metal Detection System

2010Heavy Metal Gang Captured By Capsule

Cop

2011Synthesis of Synthetic Micro-

Alkanes('Synfuels') in Engineered Escherichia coli

2012Rice Guardian& bio logic gate

2013 iGEM KOREA_U_SEOUL

KOREA_U_SEOUL

2013 iGEM KOREA_U_SEOUL

Background

Design & Parts

Results

Application & Im-

provement

Human Practice

Background

2013 iGEM KOREA_U_SEOUL

CO2 H2O HCO3- H+

2013 iGEM KOREA_U_SEOUL

CO32- 2H+

Des ign & Pa r t s

2013 iGEM KOREA_U_SEOUL

To design Pearl-coli producing nacrein on the cell surface.

2013 iGEM KOREA_U_SEOUL

2013 iGEM KOREA_U_SEOUL

pBAD BBa_K1146000YfaL_Nacrein(WT)

pBAD BBa_K1146001YfaL_Nacrein CA

pBAD BBa_K1146002YfaL_Nacrein DR

pBAD BBa_K1146003YfaL_Nacrein R

CA CAR

CA

R

CA CA

Fig2. parts

Inhibit forming CaCO3

Inhibit forming CaCO3

Forming CaCO3

Forming CaCO3

Why do we use 4 parts ??

To check the function of CA and R !!

Q.

A.

Resu l t s

2013 iGEM KOREA_U_SEOUL

1. Cloning

4 types of nacrein clones→ pB4 vector modified pET vector for protein purifi-cation

2. Expression test & Protein purifi-cation → Check protein expression

2013 iGEM KOREA_U_SEOUL

NA: NacreinDR: Deleted RepeatCA: Carbonic anhydraseR: Repeat

M NA(S) NA(I) DR(S) DR(I) CA(S) CA(I) R(S) R(I)

130

95

72

55

43

3. CA activity of nacrein protein

CO2 + H2O → HCO3-+ H+

Carbonic Anhy-drase

If nacrein has carbonic anhydrase activity, ->pH will be lowered.

Expectation

2013 iGEM KOREA_U_SEOUL

4. CaCO3 precipitation

Ca2+ + HCO3-→ CaCO3 + H+

If Nacrein inhibits CaCO3 precipitation, -> pH will slowly drop.

2013 iGEM KOREA_U_SEOUL

0 2 4 6 8 10 12 14 16 18 206.5

7

7.5

8

8.5

9

CaCO3 precipitation

NARTris

Time (min)

pH

Expectation

App l i ca t i on & Improvement

2013 iGEM KOREA_U_SEOUL

2013 iGEM KOREA_U_SEOUL

1. 4 types of nacrein clones

2. Carbonic anhydrase activity of nacrein

3. The role of nacrein in precipitation of CaCO3

2013 iGEM KOREA_U_SEOUL

The problem of tag Prokaryote hostchang

eEukaryote

Calcium Supplement Reducing CO2

GlobalWarming

2013 iGEM KOREA_U_SEOUL

Our research can be basement for making real pearl

2013 iGEM KOREA_U_SEOUL

Human Prac t i ce

2013 iGEM KOREA_U_SEOUL

CreativeChallenger Program (CCP)Seoul 2013 Nolto EXPO KUAS homepageCote d'Ivoire

2013 iGEM KOREA_U_SEOUL

Q&A

2013 iGEM KOREA_U_SEOUL