Temporary Presence of Myofibroblasts in Human Elbow Capsule After Trauma

by Job N. Doornberg, Tjalling Bosse, Mark S. Cohen, Jesse B. Jupiter, David Ring, and Peter Kloen

J Bone Joint Surg AmVolume 96(5):e36

March 5, 2014

©2014 by The Journal of Bone and Joint Surgery, Inc.

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Acute trauma for less than seven days: a focus of organizing granulation tissue with extravasation of red cells, influx of macrophages, and inconspicuous surface synovium.

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Staining for α-SMA revealed no expression of α-SMA in fibroblast-like cells; of note, smooth muscle cells located in perivascular areas stain for α-SMA indicating specificity of staining (25×).

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Posttraumatic elbow contracture: fibrosis with increased dense plump collagen matrix with fibroblasts and no neovascularization (200×).

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.

Staining for α-SMA revealed no expression of α-SMA in fibroblast-like cells; of note, smooth muscle cells located in perivascular areas did stain for α-SMA, indicating that our staining

protocol for α-SMA was specific (200×).

Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36

©2014 by The Journal of Bone and Joint Surgery, Inc.