Open Forum Infectious Diseases
M A J O R A R T I C L E
Ten-Year Experience of Cutaneous and/or SubcutaneousInfections Due to Coelomycetes in FranceSarah Guégan,1,2,3,a Dea Garcia-Hermoso,4,5,a Karine Sitbon,4,5 Sarah Ahmed,6 Philippe Moguelet,7 Françoise Dromer,4,5 and Olivier Lortholary4,5,8,9;the French Mycosis Study Group1Assistance Publique-Hôpitaux de Paris, Service de Dermatologie, Hôpital Tenon, 2Université Pierre et Marie Curie-Paris VI, 3Institut National de la Santé et de la Recherche Médicale Unité 938, SaintAntoine Research Center, 4Institut Pasteur, Unité de Mycologie Moléculaire, Centre National de Référence Mycoses Invasives et Antifongiques, and 5Centre National de la Recherche Scientifique Unitéde Recherche Associée 3012, Paris, France; 6CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; 7Assistance Publique-Hôpitaux de Paris, Service d’Anatomo-Pathologie, Hôpital Tenon,8Assistance Publique-Hôpitaux de Paris, Centre d’Infectiologie Necker Pasteur, Hôpital Necker-Enfants Malades, and 9Université René Descartes-Paris, France
Background. Coelomycetes are rarely but increasingly reported in association with human infections involving mostly skin andsubcutaneous tissues, both in immunocompetent and immunocompromised patients. Coelomycetes constitute a heterogeneousgroup of filamentous fungi with distinct morphological characteristics in culture, namely an ability to produce asexual spores withinfruit bodies.
Methods. We included all cases of proven primary cutaneous and/or subcutaneous infections due to coelomycetes received foridentification at the French National Reference Center for Invasive Mycoses and Antifungals between 2005 and 2014. Eumycetoma,chromoblastomycosis, and disseminated infections were excluded.
Results. Eighteen cases were analyzed. The median age was 60.5 years. In all cases, patients originated from tropical or subtrop-ical areas. An underlying immunodepression was present in 89% of cases. Cutaneous and/or subcutaneous lesions, mainly nodules,abscesses, or infiltrated plaques, were observed in distal body areas. Isolates of different genera of coelomycetes were identified:Med-icopsis (6), Paraconiothyrium (3), Gloniopsis (3), Diaporthe (3), Peyronellaea (2), Lasiodiplodia (1). Lesion treatment consisted ofcomplete (10) or partial (2) surgical excision and/or the use of systemic antifungal therapy, namely voriconazole (5) and posacona-zole (4). Literature review yielded 48 additional cases of cutaneous and/or subcutaneous infections due to coelomycetes.
Conclusions. Infectious diseases physicians should suspect coelomycetes when observing cutaneous and/or subcutaneous infec-tions in immunocompromised hosts from tropical areas; a sequence-based approach is crucial for strains identification but must besupported by consistent phenotypic features; surgical treatment should be favored for solitary, well limited lesions; new triazoles maybe used in case of extensive lesions, especially in immunocompromised patients.
Keywords. coelomycetes; cutaneous phaeohyphomycosis; Medicopsis romeroi; Paraconiothyrium sp; subcutaneous abscess.
In the past 20 years, the incidence of community-acquired op-portunistic infections has steadily risen, and invasive fungal dis-eases have become a growing source of morbidity and mortality.Rare and even new fungal species, among which melanizedfungi and more specifically coelomycetes, are increasingly rec-ognized as significant human pathogens [1].
Coelomycetes are a large and phylogenetically heterogeneousgroup of filamentous fungi that are grouped together on thebasis of their asexual morphs in culture, ie, their ability to pro-duce asexual spores known as conidia, within fruit bodies
named conidiomata [2]. In both immunocompetent and immu-nocompromised patients, coelomycetes have been incriminatedin various skin and soft tissue infections, namely cutaneous andsubcutaneous phaeohyphomycosis, eumycotic black-grain my-cetoma, and even 1 isolated case of chromoblastomycosis [3–6].The term phaeohyphomycosis was initially coined in 1974 todescribe various clinical manifestations caused by melanizedfungi. It is defined by the presence of dematiaceous yeast-likecells, pseudo-hyphae-like elements, hyphae, or any combina-tion of these in tissues [4].
In this study, we report 18 cases of cutaneous and/or subcu-taneous infections due to coelomycetes that answer that de-scription and can therefore be referred to as cutaneous and/orsubcutaneous phaeohyphomycoses. Our aim was to (1) bettercharacterize these rare fungal infections and their causativeagents and (2) review treatment options.
MATERIAL AND METHODS
Inclusion and Exclusion CriteriaWe performed a retrospective analysis of consecutive cases ofcutaneous and/or subcutaneous infections due to coelomycetous
Received 24 March 2016; accepted 16 May 2016.aS. G. and D. G.-H. contributed equally to this work.Correspondence: O. Lortholary, Institut Pasteur, Unité de Mycologie Moléculaire, Centre
National de Référence Mycoses Invasives et Antifongiques, 28 rue du Dr. Roux, 75724 ParisCedex 15, France ([email protected]).
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© The Author 2016. Published by Oxford University Press on behalf of the Infectious DiseasesSociety of America. This is an Open Access article distributed under the terms of the CreativeCommons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in anymedium, provided the original work is not altered or transformed in any way, and that the workis properly cited. For commercial re-use, please contact [email protected]: 10.1093/ofid/ofw106
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fungi that were received for identification at the French Na-tional Reference Center for Invasive Mycoses and Antifungals(NRCMA) from 2005 to 2014. Cases were included if theyfulfilled the following criteria: (1) proven infection accordingto the European Organization for Research and Treatment ofCancer/Invasive Fungal Infections Cooperative Group and theNational Institute of Allergy and Infectious Diseases MycosesStudy Group (EORTC/MSG) criteria with isolate recoveryfrom abscess drainage, skin biopsy, or subcutaneous tissuesamples; (2) absence of dissemination and microbiologicallydocumented deep organ involvement (ie, primary cutaneousand/or subcutaneous infections). Cases of eumycetoma andchromoblastomycosis were excluded. A case of phaeohypho-mycosis was defined by clinical findings consistent with thatinfection and either histopathological evidence of a melanizedfungus or a culture positive for a melanized fungus and knownagent of phaeohyphomycosis. Procedures were in accordancewith the Helsinki Declaration.
Questionnaire and Pathological AnalysisA specific questionnaire was sent to collect epidemiological,clinical, mycological, and therapeutic data as well as follow-upinformation. Missing information and ambiguous answers werechecked by phone with the physician and microbiologist. Tissuebiopsies and histological sections (hematoxylin-eosin [HE],Gomori methenamine silver, and periodic acid-Schiff [PAS] stain-ings) were obtained from pathology laboratories and reanalyzedby a dermatopathologist. When necessary, Fontana-Masson stain-ing was performed to visualize hyphae pigmentation.
Mycological IdentificationAccording to standard practice at the NRCMA, species identi-fication was performed by a polyphasic approach. The purity ofall isolates was checked by obtaining single isolated colonies onSabouraud chloramphenicol agar medium. Colonies were sub-cultured onto 2% malt agar, potato carrot agar (PC), oatmealagar (OA) tubes, or autoclaved straw pieces on 2% water agarplates and incubated at 30°C under near-ultraviolet light or at25°C to promote sporulation. Microscopic preparations weremounted in cotton blue from cultures sporulating on the differentmedia. Molecular identification was performed by sequencing theITS1-5.8S-ITS2 (ITS) region of the ribosomal deoxyribonucleicacid (rDNA), the D1-D2 domain of the large subunit rDNA(28S), and a small region of the elongation factor (EF)-1α andof the β-tubulin (TUB) genes (described in Supplementary Data).
Antifungal Susceptibility DeterminationIn vitro susceptibility testing was performed according to theEuropean Committee on Antimicrobial Susceptibility Testing(EUCAST) procedure [7]with some modifications [8]. Seven an-tifungal agents were included: amphotericin B, triazoles (itracona-zole, voriconazole, posaconazole), echinocandins (caspofungin,micafungin), and terbinafine. All strains were subcultured onPC or OA for 7 to 30 days at 25°C and 30°C. Conidia were
then collected in water, and the suspension was adjusted to2–5 × 105 colony-forming units/mL.
Review of Reported CasesWe reviewed all cases of cutaneous and/or subcutaneous infec-tions due to coelomycetes published in the literature from 1970to 2015, excluding eumycetoma and chromoblastomycosis. Thekeywords used for this search were as follows: phaeohyphomyco-sis, and cutaneous, subcutaneous, abscess, cyst, skin, coelomy-cetes, Phoma, Pleurophoma, Rhytidhysteron rufulum, Medicopsisromeroi, Pyrenochaeta, Paraconiothyrium, Phomopsis, Pleuropho-mopsis, Lasiodiplodia, Colletotrichum, Coniothyrium, Micro-sphaeropsis, Nattrassia mangiferae, Gloniopsis, and Diaporthe.
RESULTS
Epidemiological Characteristics of the PatientsAmong a total of 31 fungal infections due to coelomycetes andreceived for identification at the NRCMA, 18 cases of provencutaneous and/or subcutaneous infections were analyzed(Table 1). Patients’ median age was 60.5 years (47–78 years).The sex ratio was 3.5:1 (14 of 18, ie, 78% of patients were male).
Geographical DistributionPatients all originated from tropical and subtropical regions,and most had traveled there in the past 18 months: Africa (10cases), Asia (3 cases), West Indies (5 cases). Five patients (28%)were from rural areas, engaged in farming or with a history ofsoil or plant trauma 1 to 3 months before lesion appearance.However, 3 immunocompromised patients without traumahistory had not left France for 5 to 10 years before lesionappearance.
Underlying Diseases and Risk FactorsUnderlying diseases were reported in 16 cases (89%). Nine pa-tients (50%) received solid organ transplants (SOT) (8 kidneys,1 liver); 6 patients suffered from diabetes mellitus, and 2 pa-tients suffered from hematological malignancies. Topical/oralsteroids and immunosuppressive agents (mycophenolate mofe-til, tacrolimus, cyclosporine) were used in 11 and 9 cases, re-spectively. Two patients (11%) had no apparent underlyingdisease.
Clinical Signs and SymptomsFourteen patients (patients 1 to 14) displayed subcutaneous le-sions, usually 1 solitary nodule (Figure 1), sometimes associatedwith local inflammation and mimicking an abscess (8 cases).One patient had multiple cutaneous and subcutaneous lesionson the knee (patient 13; Figure 2), and 1 patient had an apon-eurotic cyst (patient 14). Three patients (patients 15 to 17)presented with a cutaneous form of the disease, exhibiting in-filtrated, pigmented, budding, or necrotic plaques, scaly andwart-like when localized on the foot sole. Solitary lesions werethe most frequent (12 patients, 67% of cases). Two patientsdisplayed concomitant cutaneous/subcutaneous infections
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Table 1. Clinical and Epidemiological Characteristics of 18 Human Cutaneous and/or Subcutaneous Infections Due to Coelomycetes Seen Between 2005 and 2014 in France
Treatment
Patient
Sex,Age
(Years)InjuryHistory
GeographicalArea
Underlying RiskFactors Lesion Type
Body Site(Lesion
Characteristics) Histology
DirectMicroscopicExamination Culture
Agent (Dosage,mg/day, Duration) Surgery Outcome
Follow-Up
1 F, 59 Sri Lanka/France
Diabetes mellitus/PolymyalgiarheumaticaPrednisone
Subcutaneous Foot (1 nodule) Pigmentedhyphae,gran inf
Hyphae Medicopsisromeroi
None Totalexcision
Cured 8 y
2 F, 73 India/France Giant cell arteritisPrednisone
Subcutaneous Foot (1 abscess),leg (2 cysticnodules)
Pigmentedhyphae,gran inf
Negative M romeroi VCZ (400, 3 wk) Totalexcision
Cured 7 y
3 M, 65 West Africa/France
Renal graftIS therapy: Tac, MMF,
prednisone
Subcutaneous Knee (1 abscess) Aspecificchronicinf
Negative M romeroi POSA (800, 1 mo) None Cured 6 y
4 F, 47 West Africa/France
Diabetes mellitus Subcutaneous Foot (1 abscess) Pigmentedhyphae,gran inf
Not done M romeroi None Totalexcision
Cured 1 y
5 M, 53 Pakistan /France Liver graftIS therapy: Tac, MMF,
prednisone
Subcutaneous Foot (1 abscess) Not done Hyphae M romeroi POSA (800, 2 wk)followed byLAMB (3 mg/kg, 2 mo)
2 abscessdrainages
RelapseunderPOSA.Cured byLAMB+totalexcision
10 mo
6 M, 54 Farmer /injury
West Indies/France
Chronic hepatitis C Subcutaneous Forearm(1 abscess)
Pigmentedhyphae,gran inf
Hyphae Gloniopsis sp None Totalexcision
Cured 8 y
7 M, 63 West Africa/France
Renal graftIS therapy:
cyclosporine
Subcutaneous Hand (1 abscess) Not done Hyphae Gloniopsis sp POSA (800, 2 mo) Relapsetreated bytotalexcision
Relapse 19mo afterPOSA.Cured byexcision
5 y
8 M, 53 Sheperd/injury
West Africa/France
Acute B-cell leukemia,neutropenia
Subcutaneous Finger(1 infiltratedplaque), foot(2 abscesses)
Not done Hyphae Peyronellaeagardenia
LAMB (3 mg/kg,6 wk) followedby VCZ (400, 6wk)
None Cured 6 y
9 M, 58 Farmer West Africa/France
Renal graftIS therapy: Tac, MMF,
prednisoneDiabetes mellitus
Subcutaneous Foot (1 abscess) Pigmentedhyphae,gran inf
Hyphae P gardeniae VCZ (400, 13 mo) Totalexcision
Cured 5 y
10 [9] M, 71 Gardener West Indies(Guadeloupe)/France
Renal graftIS therapy: Tac, MMF,
prednisone
Subcutaneous Elbow (1 nodule) Not done Hyphae Paraconiothyriumcyclothyrioides
LAMB (3 mg/kg,4 wk) and VCZ(400, 7 wk)
None Died ofunderlyingcondition
2 mo
11 M, 78 West Indies(Guadeloupe)
Diabetes mellitus Subcutaneous Foot (1 nodule) Not done Hyphae Diaporthe sp ITRA (200, 18 mo) None Relapse 6 moafter ITRA
2 y
12 M, 51 Foottrauma
West Africa/France
Renal graftIS therapy: Tac, MMF,
prednisoneDiabetes mellitus
Subcutaneous Finger (1 nodule),foot (2nodules)
Pigmentedhyphae,gran inf
Hyphae Diaportheraonikayaporum
None Totalexcision
Cured 2 y
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Table 1 continued.
Treatment
Patient
Sex,Age
(Years)InjuryHistory
GeographicalArea
Underlying RiskFactors Lesion Type
Body Site(Lesion
Characteristics) Histology
DirectMicroscopicExamination Culture
Agent (Dosage,mg/day, Duration) Surgery Outcome
Follow-Up
13 M, 62 West Africa/France
Renal graftIS therapy: Tac,
prednisone
Subcutaneous Knee (multiplenodules withdiffuse skininfiltration)
Pigmentedhyphae,gran inf
Hyphae Ascomycete orderPleosporales
POSA (800, 4 y) 3 rounds ofpartialexcision
2 earlyrelapses inthe first 6mo
4 y
14 M, 70 West Africa/France
None Subcutaneous Elbow (1aponeuroticcyst)
Pigmentedhyphae,gran inf
Not done Gloniopsis sp None Totalexcision
Cured 3 mo
15 M, 55 La Réunion/France
Renal graftIS therapy: Tac, MMF,
prednisoneDiabetes mellitusChronic hepatitis B and
C/cirrhosis
Cutaneous Foot sole (1pigmentedbuddinginfiltratedplaque withcentralnodule)
Pigmentedhyphae,gran inf
Hyphae P cyclothyrioides LAMB (3 mg/kg,4 wk) and
Caspofungin(50, 1 wk)
None Died ofunderlyingcondition
1 mo
16 [10] M, 68 West Indies(Martinique)
B cell lymphoma,chemotherapy,methylprednisolone,neutropenia
Cutaneous Foot sole(1 pigmented
infiltratedplaque)
Not done Hyphae P cyclothyrioides VCZ (200, 10 wk)andcorticosteroidsdiscontinuation
None Lesionregression.Died ofunderlyingcondition
3 mo
Cutaneous Heel (1pigmentedinfiltratedplaque)
Not done Hyphae Diaporthe sojae
17 [11] M, 66 Central Africa/France
Renal graftIS therapy: Tac, MMF,
prednisolone
Cutaneous Foot sole (1plantar wart)
Pigmentedhyphae,gran inf
Hyphae M romeroi Reduction of IStherapy
total excision Cured 4 y
18 F, 47 West Indies(Martinique)
2nd/3rd degree burnlesions over 60% oftotal BSA
Cutaneous Forearm (3ddegreenecrotic burnlesion)
Not done Negative Lasiodiplodiatheobromaespeciescomplex
None Excision ofnecrotictissues
Died ofunderlyingcondition
2 wk
Abbreviations: BSA, body surface area; gran, granulomatous; inf, inflammation; IS, immunosuppressive; ITRA, itraconazole; LAMB, liposomal amphotericin B; MMF, mycophenolate mofetil; POSA, posaconazole; Tac, tacrolimus; VCZ, voriconazole.
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Figure 1. Patient 1. (A and B) Painless subcutaneous cyst of the left foot containing a puriform liquid in a 59-year-old woman with polymyalgia rheumatica treated bycorticosteroid therapy. (C) Hematoxylin-eosin staining showed a deep dermal abscess mixed with granulomatous inflammation (×40). The black triangle labels the cystlumen. (D) Hematoxylin-eosin staining (×1000) with high magnification of a multinucleated cell (black star) and pigmented fungal hyphae (black arrows) (×1000). (E) Gomorimethenamine silver stain and (F) periodic acid-Schiff staining ([E], ×400; [F], ×1000) revealed globose or elongated septate hyphal elements (black arrows).
Figure 2. Patient 13. Pigmented infiltrated plaque of the right knee associated with diffuse subcutaneous infiltration in a 62-year-old kidney transplant recipient. (A) Full and(B) close-up views (courtesy of Camille Frances). (C and D) Hematoxylin-eosin staining revealed a dense dermal infiltrate with granulomatous inflammation, associating neu-trophils, lymphocytes, epithelioid, and multinucleated cells, as well as pigmented fungal hyphae ([C], ×100; [D], ×400). (E) Periodic acid-Schiff staining showed septate fungalhyphae (×1000). (F) Fontana-Masson staining confirmed the pigmented character of fungal structures (×1000).
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involving 2 (Paraconiothyrium cyclothyrioides and Phaeoacre-monium parasiticum [9], patient 10) or 3 fungi (Phomopsislongicolla reidentified as Diaporthe sojae, P cyclothyrioides andCunninghamella bertholletiae [10], patient 16). Lesions involvedexclusively distal areas of the upper limbs in 7 cases (39%) andthe lower limbs in 13 cases (72%). The foot was the site mostfrequently involved (14 lesions).
In all 11 cases in which a pathological examination of skintissue was performed, an aspect highly suggestive of infectionwas observed with a granulomatous dermal infiltrate containingepithelioid and multinucleated giant cells. In 10 of 11 cases,stainings revealed elongated septate and branched hyphae orglobose fungal structures (HE, PAS, and Gomori methenaminesilver stainings; Figures 1 and 2). Pigmentation of the fungalstructures was confirmed using HE and Fontana-Massonstainings.
Mycological IdentificationTwenty-two isolates were available for the 18 patients, but thesame fungus was identified in the 4 cases in which we had 2
isolates. Microscopy of the initial cultures revealed septate-melanized hyphae in the majority of isolates (Figure 3). Pycnidialconidiomata were seen for 13 of 22 isolates after at least 3 weeksof subculture on special media (Supplementary Table S1). In par-allel, a multilocus sequence-based analysis was performed forall clinical isolates, resulting in the identification of 11 isolatesto the species level:Mromeroi (6 cases),P cyclothyrioides (3 cases),Diaporthe raonikayaporum (1 case), and D sojae (1 case). Iden-tification to the genus level was achieved for 4 isolates: Diaporthesp (1 case) and Gloniopsis sp (3 cases). One isolate belonged tothe Lasiodiplodia theobromae species complex, and a possibleidentity of Peyronellaea gardeniae was found for 2 isolates. Final-ly, the identity of isolate CNRMA11.1115 remained uncertaindue to the absence of sequence entries on the curated databasesfor known taxa (Supplementary Table S1).
In Vitro Antifungal Susceptibility TestingMinimum inhibitory concentrations (MICs) were determinedfor the 11 strains that produced enough conidia (Supplementa-ry Table S2). Low MICs of amphotericin B (0.06 to 1 µg/mL),
Figure 3. Macroscopic aspect of Medicopsis romeroi on oatmeal agar (OA), 28°C, 14 days (A); immersed pycnidium of strain CNRMA11.1115 on OA medium (B); conid-iophores and conidiogenous cells from a pycnidium of M romeroi (C) small, hyaline conidia of Gloniopsis sp strain (D); mature, septate, striated conidia of Lasiodiplodiatheobromae species.
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Table 2. Clinical and Epidemiological Characteristics of Reported Human Cutaneous and/or Subcutaneous Infections Due to Coelomycetes
Author (andReference)
Year ofReport
Sex,Age inYears Injury History
GeographicalArea Underlying Risk Factor
Type of Lesion(SC or C) Body Site Culture Treatment Outcome
Follow-Upa
Bakerspigel[S2]
1970 F, 22 Farmer Ontario, Canada Topical steroids C: Erythematousnodule withpustular lesions
Leg Phoma hibernica Oral griseofulvin Regression 5 y
Young [S3] 1973 F, 42 Jamaica/ UnitedStates
Renal transplant SC: Subcutaneouscystic lesion
Heel Phoma sp Excision Cured 6 mo
Gordon [S4] 1975 M, 4 None C: Superficialcrusted lesion
Ear Phoma sp Oral griseofulvin Cured ND
Bakerspigel[S5]
1981 M, 1.5 Ontario, Canada None C: Perioral crustedlesion
Face Phoma eupyrena Topical clotrimazole Cured 2 y
Shukla [S6] 1984 F, 18 India Typhoid fever C: Superficialpapulovesicularlesions
Face Phoma minutispora Topical clotrimazole Cured 1 mo
Shukla [S6] 1984 M, 20 Farmer India Corticosteroids (chronicsinusitis)
C: Superficialmaculopapules
Neck P minutispora Topical clotrimazole Cured 20 d
Baker [S7] 1987 M, 75 Farmer DominicanRepublic
Diabetes mellitus/Corticosteroids(myasthenia gravis)
SC: Subcutaneouslesion
Foot Phoma minutella Amputation Cured 1 y
Stone [S8] 1988 M, 25 Texas None SC: Subcutaneouscystic lesion
Forearm Phoma sp Excision/Oralketoconazole
Cured 15 mo
Dooley[17, 18]
1989 F, 56 Gardener Texas Diabetes mellitus/ Cardiactransplant
SC: Subcutaneousnodules
Thigh,knee,wrist
Pleurophomapleurospora
Reclassified asParaconiothyriummaculicutis
Excision/ Topicalmiconazole
Cured ND
Rai [S9] 1989 M, 24 India None C: Superficialpapulovesicularlesions
Face, neck,hands
Phoma sorghina Topical miconazole Cured 1 mo
Rai [S9] 1989 M, 19 India None C: Superficialmacular lesions
Face P sorghina Topical miconazole Cured 1 mo
Chabasse[S10]
1995 M, 74 Farmer France Corticosteroids (asthma) SC: Subcutaneousabscess
Leg Pleurophomopsislignicolla Petr
Excision. Relapsetreated by a secondexcision.
Relapse after 12mo.
12 mon
Rosen [S11] 1996 F, 24 Vacations on afarm
Texas Topical steroids C: Infiltrated plaque Face Phoma sp Oral ketoconazole Cured 2 y
Hirsh [S12] 1996 M, 45 Farmer Hawaii None C: Infiltrated plaque,nodules
Hand Phoma sp Oral itraconazole Regression ND
Maslen [S13] 1996 F, 40 Intramuscularinjections inbuttock
Cambodia/Australia
None SC: Induratedplaque withcentralsubcutaneousabscess
Buttock Lasiodiplodiatheobromae
Debridement Cured 5 mo
Zaitz [S14] 1997 M, 63 Brazil Corticosteroids (sarcoidosis) C: Infiltrated plaque,nodules
Sternalregion,hand
Pleurophoma cava Amphotericin B/Oralitraconazole
Cured ND
Arrese [S15] 1997 M, 53 Owner of abakery
Morocco/Belgium
Topical steroids/Shortcorticosteroid therapies(urticaria, hay fever)
C: Scaly plantarlesion
Foot Phoma sp Topical bifonazole/topical ketoconazole
Persistence Lost tofollow-up
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Table 2 continued.
Author (andReference)
Year ofReport
Sex,Age inYears Injury History
GeographicalArea Underlying Risk Factor
Type of Lesion(SC or C) Body Site Culture Treatment Outcome
Follow-Upa
Sigler [19] 1997 M, 73 Arizona Diabetes mellitus/Corticosteroids therapy
SC: Subcutaneousabscesses andinfiltratedplaques
Arm,forearm
Nattrassiamangiferae
Topical miconazole/Amphotericin B
Relapse ND
Guarro [S16] 1998 M, 56 Farmer,traumaticinjury
Brazil Diabetes mellitus/corticosteroids
SC: Several nodularsolitary orconfluentlesions, macularlesions
Forearm,elbow
Colletotrichumgloeosporioides
None Persistence Died ofothercause
Oh [S17] 1999 M, 77 Farmer Korea Topical steroids C: Indurated plaque Forearm Phoma sp Oral itraconazole Regression ND
Guarro [S18] 1999 F, 59 Spain None C: Scaly, infiltratedplaque withinflammatoryborder
Shoulder Microsphaeropsisolivacea
Topical clotrimazole/oral terbinafine
Cured 7 mo
O’Quinn [20] 2001 M, 34 Cactusinoculation
Tennessee Acute lymphocytic leukemia/Chemotherapy
SC: Subcutaneoustender nodule
Forearm C gloeosporioides Amphotericin Bfollowed by oralitraconazole
Cured ND
O’Quinn [20] 2001 M, 47 Mississippi Non-Hodgkin lymphoma/Chemotherapy/ autologousstem cell transplantation
SC: Subcutaneoustender nodulewith centralpustule
Arm Colletotrichumcoccodes
Amphotericin Bfollowed by oralitraconazole
Cured ND
Castro [S19] 2001 M, 34 Gardener Brazil Renal transplant SC: Subcutaneousnodule
Leg Colletotrichumcrassipes
Total excision Cured ND
Miele [S20] 2002 M, 60 Gardener Washington DC Diabetes mellitus/Renaltransplant
SC: Subcutaneousabscess
Knee Coniothyrium-Microsphaeropsiscomplex
Broad debridement/Oral itraconazole
Cured ND
Girard [S21] 2004 M, 45 West Africa/France
Leprosy SC: Multiple painfulnon-inflammatorysubcutaneousnodules
Legs (2),foot (2)
Pyrenochaetaromeroi
Surgical excision/Abscess drainage/oral itraconazole
Cured 1 y
Summerbell[S22]
2004 F, 50 Outdoor injury Jamaica None SC: Ulcer Leg Lasiodiplodiatheobromae
Broad debridement Cured 6 mo
Siu [S23] 2004 M, 49 Traumaticabrasion
Hawaii Diabetes mellitus/Hearttransplant
SC: Annular andnodular plaques
Legs,Knees
Coniothyrium-Microsphaeropsiscomplex
Several relapsesfollowing excisionand oral treatment(fluconazole,itraconazole).
Treated by excision andAmphotericine B
Cured 4 mo
Godoy [S24] 2004 M, 65 Lived in ruralarea
Brazil None C: Desquamativeinterdigitallesions
Feet N mangiferae ND ND ND
Padhye [S25] 2004 M, 41 West Africa Diabetes mellitus/AIDS/chronic hepatitis/activetuberculosis
SC: Tender, mobile,subcutaneousabcess
Arm Pleurophomopsislignicolla
Abscess drainage Healed ND
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Table 2 continued.
Author (andReference)
Year ofReport
Sex,Age inYears Injury History
GeographicalArea Underlying Risk Factor
Type of Lesion(SC or C) Body Site Culture Treatment Outcome
Follow-Upa
Pendle [S26] 2004 M, 80 Australia Diabetes mellitus/Inflammatorydemyelinatingpolyneuropathy/
Immunosuppressive therapy
C: Painlessgranulomatousplaque
Hand Microsphaeropsisarundinis
Terbinafine Cured 2.5 y
Pendle [S26] 2004 M, 56 Australia Diabetes mellitus/ Ankylosingspondylarthropathy/
Immunosuppressive therapy
SC: Necrotic ulcers Feet M arundinis Amputation anditraconazole
Cured 10 mo
Suh [S27] 2005 M, 19 Korea Unknown status C: Verrucous plaque Face Phoma sp AmB Regression ND
Balajee [21] 2007 M, 3 ND Liver transplant C: Crusted nodules Leg Paraconiothyriumcyclothyrioides
ND ND ND
Badali [22] 2010 F, 45 India None SC: Verrucousplaque,subcutaneouscyst
Forearm Pyrenochaetaromeroi
Excision Cured 1 y
Khan [S28] 2011 F, 47 India/ Kuwait Acute lymphoblasticleukemia/Chemotherapy
SC: Subcutaneousnodule withcentral necrosis
Finger P romeroi Cyst drainage Partial regression ND
Gordon [23] 2012 M, 49 Texas Renal transplant/ Diabetesmellitus
C: Crustedulceratedplaques
Legs P cyclothyrioides No response toVoriconazole.Posaconazole
Cured ND
Severo [S29] 2012 M, 53 Brazil Lung transplant SC: Necroticulceratedsubcutaneouscyst
Knee C gloeosporioides Total excision Cured Died
Mattei [24] 2013 M, 43 Farmer Brazil Renal transplant/ Diabetesmellitus
C: Induratedplaques
Arm, leg Diaporthephaseolorum
Oral itraconazole andsurgical excision
Cured 5 mo
Hsiao [25] 2013 M, 78 Farmer Taiwan None C: Verrucous plaque Forearm,dorsalhand
P romeroi No response to oralitraconazole andsurgical excision.Amphotericin B
Cured 6 mo
Hall [S30] 2013 M, 70 Florida Renal transplant C: Crusted,ulcerated plaqueand papules
Finger,forearm
M arundinis Posaconazole Cured 6 mo
Mahajan [26] 2014 M, 72 India Diabetes mellitus SC: Subcutaneousswelling
Foot Rhytidhysteronrufulum
No response to acombination ofsurgical excision,itraconazole andterbinafine.Intralesionalliposomalamphotericin B
Cured 1 y
Cutaneous
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Table 2 continued.
Author (andReference)
Year ofReport
Sex,Age inYears Injury History
GeographicalArea Underlying Risk Factor
Type of Lesion(SC or C) Body Site Culture Treatment Outcome
Follow-Upa
Chan [S31] 2014 M, 55 China Renal transplant SC: Painlessnodularsubcutaneouscyst
Thigh P romeroi Several relapses afterrepeated attempts attotal excision.
Oral Itraconazolecontinued untildeath.
Relapse aftertapering ofitraconazole.Remission onresuming fulldose.
5 y
Ogawa [S32] 2014 M, 68 Brazil Renal transplant SC: Papulo-nodularlesion
Finger C gloeosporioides Total excision Cured 1 y
Asahina [27] 2015 F, 57 Japan Systemic lupuserythematosus
Autoimmune hepatitisImmunosuppressive therapy
C: Erythematousscaling plaquesand nodules
Finger,forearm,knee, leg,abdomen
M arundinis Itraconazole,fluconazole,liposomalamphotericin Bineffective.
Local thermotherapy.
Resolution afterlocal therapy
ND
Asahina [27] 2015 F, 74 Japan Temporal arteritisHypogammaglobulinemiaImmunosuppressive therapy
Diabetes mellitus
C: Erythematousinduratedplaques andpapules
Hand M arundinis Itraconazole and localthermotherapy.
Regression ND
Papacostas[S33]
2015 M, 59 Inoculation Kenya/ Australia None SC: Subcutaneousswelling
Foot Lasiodiplodiatheobromae
Excision andVoriconazole.
Cured 3 mo
Yadav [S34] 2015 F, 50 India Diabetes mellitus SC: Painlesssubcutaneouscyst
Foot P romeroi Drainage andItraconazole
Cured 3 mo
Abbreviations: AIDS, acquired immune deficiency syndrome; AmB, amphotericin B; C, cutaneous; ND, not described; SC, subcutaneous.
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voriconazole (0.03 to 0.5 µg/mL), and terbinafine (0.06 to 1 µg/mL)were observed for all the strains tested with the exception ofspecies CNRMA13.515, which exhibited a terbinafine MIC of4 µg/mL. The P cyclothyrioides and the 2 P gardeniae isolatesexhibited low MICs of all antifungals tested except for echino-candins and posaconazole, respectively. The 3M romeroi strainsand the 1 belonging to the L theobromae species complex hadhigh itraconazole MICs (4 to ≥8 µg/mL). The lowest triazoleand echinocandin MICs were observed for P cyclothyrioidesand D sojae, respectively.
Treatment and Follow-UpSystemic antifungals were prescribed in 11 of the 18 cases(61%) for a median duration of 2.5 months: voriconazole (5cases), posaconazole or liposomal amphotericin B (4 caseseach), and itraconazole (1 case). In 1 patient, liposomal am-photericin B therapy was followed by voriconazole administra-tion. Another patient was switched to liposomal amphotericinB after 2 weeks of inefficient posaconazole treatment. Liposo-mal amphotericin B was combined with caspofungin or vori-conazole (1 case each). Six patients were treated by antifungalsalone. Surgery was performed in 12 patients: total excision (10cases) or partial excision (2 cases). It was the sole treatment in7 cases (39%).
The mean follow-up was 36 months (2 weeks–96 months).Four patients (22%) died of the underlying disease during thefirst 3 months. Regression or complete cure was obtained in13 of the remaining 14 cases where follow-up was available.Seven patients were cured by excision alone, which was thefirst-line treatment in 6 cases. Of the 6 patients treated by anti-fungal drugs only, 2 relapsed. Of note, patient 7 relapsed 19months after a 2-month treatment with posaconazole and wascured by total excision of the relapsing solitary lesion. Of the 4patients treated by combined surgery and antifungals, 2 patientsrelapsed but were eventually cured by a second or third line ofcombined treatment. The diffuse subcutaneous infiltration of thelower limb of patient 13 was controlled by the combination of 3partial surgical procedures and a prolonged high-dose regimen ofposaconazole.
DISCUSSION
Coelomycetes correspond to an artificial group of ascomyceteand few basidiomycete fungi able to produce spores (conidia)within fruit bodies (conidiomata) [6, 12]. To date, approximate-ly 1000 genera and 7000 species are included in this group [13].These fungi have been reported as plant pathogens [14] and im-plicated in animal and human infections [6, 15].However, in thepast, reports have been limited by the lack of correct identifica-tion of these pathogens [1]. The ongoing taxonomical reorgani-zation of some groups of coelomycetes is an additional issue.
Melanized fungi are involved in proven infections in bothimmunocompromised and immunocompetent individuals.
In a review of 72 disseminated phaeohyphomycoses, infectionwas associated with some degree of immune dysfunction in76% of patients [16]. Similarly, 89% of our 18 patients wereimmunocompromised, with 50% of SOT recipients. Ourliterature search yielded 48 published cases of cutaneous and/or subcutaneous infections (excluding eumycetoma and chro-moblastomycosis) attributed to coelomycetes, among which29 (60%) occurred in immunocompromised patients, including12 (25%) organ transplant recipients (Table 2). It is interestingto note that 2 patients (patients 10 and 16) in our series dis-played multiple concomitant fungal infections, a finding thatunderlines the favoring role of immune suppression in theemergence of these mycoses.
All 18 of our cases occurred in patients originating from trop-ical and subtropical areas, conversely to literature review withonly 29 of 48 (60%) patients living in tropical and subtropicalregions (Table 2). Indeed, 14 (29%) cases were described inNorth American countries, United States (mostly southernstates), Canada, or European countries (Italy, Spain, France).Four (8%) cases were reported in Northern Asia (Korea, Japan).
Coelomycete infections are frequently reported after inocula-tion or in patients from rural areas engaged in farming (28% ofour patients; 39.5% [19 of 48] of literature cases). In our series,lesions involved mostly distal parts of the limbs, with foot in-volvement in 11 (61%) patients. Likewise, most of the 48 pub-lished cases occurred on exposed areas (face, neck, legs, andarms). These findings are suggestive of the role of minor traumaand inoculation, which is compatible with an environmentalsource of these fungi. Delay between inoculation and diseasemay depend on the inoculum size, the extent of the injury sus-tained, and the underlying disease. Coelomycetes are incrimi-nated as well in keratitis, with case reports suggesting the roleof corneal trauma [28]. Rare synovium or lung infections havealso been reported [29, 30].
Until recently, the study of coelomycetes phylogeny was basedon classic taxonomy that relied on morphology. However, someimportant distinctive morphological characteristics (conidiationor pigmentation) are inconstant when fungi are cultured on arti-ficial media, rendering phenotypical identification of genera andspecies difficult. The introduction of molecular techniques suchas the sequencing and analysis of fungal ribosomal operons (ITS,28S) and several protein-coding genes (actin, TUB, EF-1, cal-modulin, etc) has considerably helped in resolving species com-plexes and generic boundaries of some coelomycetes [31–37],resulting in a complete taxonomical reorganization. Many gen-era still have to be analyzed using only molecular techniques[12]. By now, the mycological community should be awarethat important nomenclatural changes are taking place sincethe publication of the “Amsterdam Declaration of Fungal No-menclature” [38], which in part abolished the separate namingof anamorphs and teleomorphs of the same fungus. The follow-ing online databases may be helpful for clarification of the
Cutaneous Infections Due to Coelomycetes • OFID • 11
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presently accepted names of fungal species (http://www.mycobank.org/ http://www.indexfungorum.org).
In this study, we performed a polyphasic approach that takesinto account morphological features, cultural characteristics,and several molecular targets. However, 7 of the strains couldnot be identified to the species level due to lack of sporulation,despite the test of varied culture conditions and lack of sequencehomology in the public databases. These strains are now includ-ed in an ongoing taxonomical study describing novel taxa incoelomycetes.
Nevertheless, in this study, we report new emerging pathogens.Gloniopsis sp was isolated in 3 patients and to date had neverbeen reported as agent of cutaneous or subcutaneous infections.Paraconiothyrium cyclothyrioides, here incriminated in 3 cases ofcutaneous plaques or abscesses, was previously described in only2 cases of skin lesions [21, 23].Of note, a reported case involvingParaconiothyrium maculicutis had initially been identified asPleurophoma pleurospora [17, 18]. The most frequently isolatedcoelomycete in our series (6 cases) was M romeroi, previouslynamed Pyrenochaeta romeroi. This fungus is usually associatedwith eumycetomas [6]. Only 6 additional cases of phaeohypho-mycoses have been attributed to this fungus (Table 2).
Limited data on the in vitro antifungal susceptibilities of coe-lomycetes are available in the literature, mainly inferred fromclinical cases, with potential variations due to the use of differ-ent methodologies [2, 3, 22]. Here all strains were tested using amicrodilution method slightly adapted from EUCAST [7, 8].Knowing the extreme phylogenetic diversity of these fungi, wewere not surprised to see marked differences in the in vitro sus-ceptibility results according to genus and species, keeping inmind that there are no defined EUCAST breakpoints for coelo-mycetes and no established correlation between MIC and clin-ical outcome. Nevertheless, low MIC values were uniformlyobtained for voriconazole. These results are in line withAhmed et al [39] study.
Treatment of coelomycete infections is obviously not stan-dardized. In the literature, therapeutic management varies ac-cording to clinical presentation (subcutaneous vs cutaneous).The vast majority (20 of 25) of subcutaneous forms (describedas abscesses, cystic lesions or ulcers) were treated by excision ordrainage (Table 2). Surgery was the only treatment in 11 of the20 cases, whereas it was combined with antifungal therapy in 9cases (itraconazole [7 cases], amphotericin B [2 cases], or keto-conazole, terbinafine, fluconazole, voriconazole [1 case each]).Excision combined with itraconazole followed by terbinafinefailed for 1 infection due to R rufulum, which was eventuallycured by intralesional amphotericin B injections [26]. The re-maining subcutaneous cases were successfully treated by anti-fungal therapy alone: systemic amphotericin B therapy (3cases) followed or not by itraconazole [19, 20]. In our series, sur-gery was performed in 10 of 14 (71%) cases, either alone (7cases) or combined with antifungal therapy. However,
conversely to literature reports in which itraconazole is thefirst-line therapy, voriconazole (5 cases), posaconazole, or lipo-somal amphotericin B (4 cases each) were mostly used in ourpatients.
Concerning the 23 cutaneous forms reported in the literature(described as plaques, papules, or crusted lesions; Table 2), sur-gical excision was performed in only 2 cases [24, 25] combinedwith itraconazole in a Diaporthe infection [24], and with ampho-tericin B in a M romeroi infection [25]. Two cases due to Micro-sphaeropsis arundinis were treated by local thermotherapy aloneor associated to itraconazole [27]. The other 19 cases were treatedby antifungal therapy only: itraconazole or topical clotrimazole (3cases each), terbinafine, griseofulvin, topical miconazole or pos-aconazole (2 cases each), ketoconazole or amphotericin B (1 caseeach). Cure was obtained in 16 of 17 cases in which evolution wasreported. Lesions persisted in 1 case treated topically. In our se-ries, surgery was performed in 2 cases; voriconazole or liposomalamphotericin B were used in 2 cases.
CONCLUSIONS
In summary, whatever the species identified, coelomycetes areresponsible for cutaneous and/or subcutaneous infections,which should be suspected in immunocompromised hosts har-boring cutaneous plaques or nodules and originating from thetropics. After skin biopsy, a polyphasic approach combiningmorphological and molecular analysis is mandatory for defini-tive mycological identification, which should be provided by anexpert laboratory. Antifungal susceptibility results vary betweencoelomycete genera and strains without any confirmed thera-peutic relevance. Therefore, surgery should be the first-linetreatment of solitary subcutaneous lesions. In case of multipleor relapsing solitary lesions, antifungal therapy (posaconazoleor voriconazole) is warranted. Liposomal amphotericin B isan alternative for management of refractory cases. In SOT recip-ients, reduction of immunosuppression should also be consid-ered, at least in case of multiple lesions.
Supplementary DataSupplementary material is available online at Open Forum Infectious Diseasesonline (http://OpenForumInfectiousDiseases.oxfordjournals.org/).
AcknowledgmentsWe thank Jean-Charles Gantier (French National Reference Center for
Invasive Mycoses and Antifungals [NRCMA]) for morphological identifica-tion and Damien Hoinard (NRCMA) for technical assistance.Potential conflicts of interest. All authors: No reported conflicts. All
authors have submitted the ICMJE Form for Disclosure of Potential Con-flicts of Interest.Members of the French Mycosis Study Group who enrolled patients in
the present study are as follows: Boulogne-Billancourt (N. Ait-Ammar,J. Dunand, B. Levy, L. Moulonguet), Clichy (V. Zarrouk), Corbeil-Essonnes(S. Kubab, C. Thépot), Guadeloupe (G. Gendrey, M. Beaubrun), Lyon(A. L. Bienvenu, S. Euvrard), Fort-de-France (N. Desbois, J. C. Meniane),Reims (S. Diallo, D. Toubas), Toulouse (S. Cassaing, J. Guitard). Paris: Bi-chat (C. Chochillon, C. Rioux), Cochin (N. Dupin, A. Paugam), Croix-Saint-Simon (V. Zeller), Necker (M. E. Bougnoux, C. Charlier), Pitié (A.
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Fekkar, J. Tourret), Saint-Louis (A. Alanio, S. Bretagne, S. Gallien, E. Raf-foux), Tenon (C. Frances).
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