ADVANCING CANADIAN AGRICULTURE AND AGRI-FOODPROJECT # AB0159CO

‘‘Testing of PhotocatalyticTechnologies for Use on Food Surfaces:

Use of Natural Products as Photocatalytic Agents’’

Nick D. Allan, M.Sc.Contract Research Manager

Biofilm Primer

The ProblemThe Need for Hard/Food Surface

Sanitizers

CFIA’s food safety yearly spending is CAD$360M1

The National Cattlemen’s Beef Association estimates $1B in beef sales are lost each year in the United States to spoilage1

The Canadian Cattleman’s Association estimates that $ 200M in sales are lost each year2.

1)Food safety Report of the US Government Accountability Office, 20052)The Cattlemen’s news Letter. Vol. 6, 2005.

The ProblemBarriers to Developing a Good Hard

Surface Sanitizer

Effectiveness Biofilms (ACAAF project # AB00008) Concentration Interference/Inactivation by environment

Duration of Efficacy Human/Food Safety

The Solution: Photo Dynamic Therapy

Contaminating Biofilm

The Solution: Photo Dynamic Therapy

Innovotech compound

Light Source

1O2

The Solution: Photo Dynamic Therapy

1O2

1O21O2

bactericidal effect

Project Overview: The Development of Sani-LuxTM

PHASE I: Food Dye Screening Physical Properties Biological activities

PHASE II: Hard Surface and Food Surface Screening (Practical Testing)

PHASE III: Filing as a Sanitizer with CFIA

PHASE I: Food Dye Screening

Evaluation of Most known food dyes Solubility (water, olive oil, sorbitol, PEG, Glycerin) Biological Activities Salmonella Staphylococcus aureus E. coli O157:H7 Listeria monocytogenes

Concentration range Light Intensity Contact Time

PHASE II: Food and Hard Surface Screening

Evaluation against E. coli and Listeriamonocytogenes Concentration range @ 16 hours Variation in effectiveness

~2.0 Log ~1.0 Log

PHASE II: Food and Hard Surface Screening

Evaluation against Salmonella and Listeriamonocytogenes Concentration range

@ 16 hours Variation in

effectiveness 3.8 Log reduction for

Listeria 2.4 Log Reduction for

Salmonella

PHASE II: Food and Hard Surface Screening

Hard Surface Evaluation against E. coli 40 ppm @ 3.5 hours 4.5 Log Reduction

PHASE III: Filing with CFIA

Registration Package: AOAC Carrier Testing Log10 Reduction Testing Label Claim Organisms (Salmonella, S. aureus, E. coli

O157:H7, Listeria monocytogenes, Campylobacter jejuni) Must Demonstrate 95% killing (~1.5 Log Reduction)

40 ppm under light intensity of 680-780 lumens using Lithonia Lighting at 24±2 °C for 3.5 hours

COA Sample Label IFU

PHASE III: Sample Label

PHASE III: IFU

Sani-LuxTM Summary

Broad spectrum kill: Listeria, Staphylococcus,Campylobacter, E. coli and Salmonella, as well asfungal pathogens such as Fusarium, Aspergillus,Pythium, and Penicillium species.

Effective on a variety of surfaces: can be used onstainless steel, ceramic, plastic, concrete, or granite.

Non-corrosive to metals encountered in household,beverage and food processing applications.

Non-toxic (Approved for use in foods at 100X the active concentration).

GREEN: contains only natural products. Natural Deodorizer: Leaves cleaned areas smelling

fresh. Continually active when a light source is present.