5

10

15

20

25

The Clone Age

Human GenomeProject

Recombinant

DNA

Gel Electrophoresis

DNA fingerprints

5

10

15

20

25

5

10

15

20

25

5

10

15

20

25

5

10

15

20

25

The first adult mammal successfully cloned was a sheep named this.

What is Dolly?

55

Dolly was cloned from what type of cell.

What is a mammary cell?

1010

These are the two most common DNA cloning vectors.

1515

What are plasmids and

bacteriophages?

Dolly was created using this type of cloning

technique.

What is nuclear transfer?

2020

Cloned goats are used to produce this substance

for human use.

2525

What is clotting factor?

The Human Genome Project was completed in this year.

55

What is 2003?

The Human Genome Project discovered that humans have this many

genes.

What is 30,000 to 40,000?

1010

This is an organism’s complete set of proteins.

What is a proteome?

1515

This percentage of human DNA differs from person to person.

What is 0.1%?

2020

The Human Genome Project revealed 8 million loci where individuals differ by only one nucleotide. These are known

as this.

What are SNPS (single nucleotide

polymorphisms?

2525

These are enzymes that cut DNA molecules at specific places.

55

What are restriction Enzymes?

These are circular pieces of bacterial DNA that replicate and can be used

as recombinant DNA vectors in genetic engineering.

What are plasmids?

1010

Fragments of DNA can join with each other if they have

complementary sequences of bases on their ends known as this.

What are sticky ends?

1515

This human protein needed by diabetics is produced through recombinant DNA technology.

What is insulin?

2020

Recombinant DNA means that the DNA from two different ones of

these have been combined.

What is species?

2525

What is size?

55

Gel Electrophoresis sorts DNA fragments

according to this.

Gel Electrophoresis makes use of the fact that DNA

molecules have this overall charge.

What is negative?

1010

DNA will move towards what end of the chamber?

What is the positive end?

1515

The gel in gel electrophoresis is made

of this substance.

What is agarose?

2020

This is the name of the solution that is poured into

the chamber.

What is TBE buffer?

2525

This is the first step in DNA fingerprinting?

What is isolate a DNA sample?

55

If a sample of DNA found at a crime scene is very small, this technique could be used to amplify (or make

multiple copies) of it.

What is PCR (Polymerase Chain Reaction)?

1010

DNA fingerprinting makes use of these segments of

repeating DNA nucleotides?

What is VNTRs (Variable number tandem repeats)?

1515

In DNA fingerprinting, cut DNA fragments are sorted

using this technology.

What is Gel Electrophoresis?

2020

The FBI uses this many VNTRs to make a positive

DNA match.

What is 13?

2525