The Daly Lab
Personnel:Postdoctoral:
•Erich Staudacher Graduate students:
•Oakland Peters•Esther Mwilaria (MS Sp 07)
Undergraduate students: •Faizan Kalwar •Mandy Hatfield •Morgan Bridi •Tyler Pittman•Aarzu Moghal •Sawan Phrabhu
Current proposals:• National Institutes of Health (R01) – NIDCD “The role of
periodic input in odor coding” PI: Kevin C. Daly 5 years; (initial score 228; in review).
• Human Frontiers in Science. “The virtual 4D antennal lobe: Representation of odor processing in space at spike-time resolution. Score: 30th (in revision for NSF ETA spring 09).
Prior funding:• National Institutes of Health (R03) – NIDCD: “Analysis of
combinatorial olfactory coding” Grant number: DC05535; 3 years, $221,000.
• McDonnell Foundation collaborative grant: “Hydrodynamic and neural filtering of spatial information in a turbulent odor plume” 3 years $105,000.
• National Institutes of Health (R01) – NIDCD “Aminergic modulation underlying olfactory plasticity” PI: Thomas A Christensen 5 years; OSU subcontract: $200,000 Grant number: DC05652.
• National Institutes of Health (P20) – NCRR “COBRE in Sensory Neuroscience” PI George Spirou; CoPI: Kevin C. Daly (RR015574-06). 5 year $10,724,104.00: Daly sub award: $1,354,457.00
Wing beating causes oscillating airflow over the antennae
Sane and Jacobson JEB 2006
Indu
ced
airs
peed
(m
/s)
Rel
ativ
e am
plitu
de
EA
G D
efle
ctio
n (m
V)
Peri-stimulus Time (sec)
2
1
0
-1
-2
2
0
-2
4
-4
i2
1
0
-1
-2
2
1
0
-1
-2
2
0
-2
4
-4
2
0
-2
4
-4
i
-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5
2
1
0
-1
-2
4
0
-4
8
-8
ii2
1
0
-1
-2
2
1
0
-1
-2
4
0
-4
8
-8
4
0
-4
8
-8
ii
20 Hz
Continuous
Frequency (Hz)
Raw
Po
we
r (m
V2 /
Hz*
10-
3 )
10 20 30 40 5010 20 30 40 50
10
5
0
10
5
0
i
16 18 20 2216 18 20 22
ii
10 15 18 20 22 25 30 34 4010 15 18 20 22 25 30 34 40
4.5
3.5
2.5
1.5
.5
-.5
4.5
3.5
2.5
1.5
.5
-.5
Stimulation frequency (Hz)A
vera
ge
po
wer
(z-
scor
e) *̂*̂
*̂*̂*̂*̂ *̂*̂ *̂*̂ *̂*̂
^̂
Oscillating odor stimulus results in oscillating electroantennogram (EAG) up to ~30Hz
RAW and 5Hz high pass filtered EAG
Power spectral densityIntegrated power
Mean integrated powerpulsed odorpulsed blank3s cont. odor
Oscillating stimulus results in oscillating local field potentials in the antennal lobe up to 72 Hz
Frequency (Hz)
Raw
Po
wer
(m
V2 /
Hz*
10-3)
10 20 30 40 5010 20 30 40 50
2
1
0
2
1
016 18 20 22
i ii
10 14 18 20 22 25 30 34 4010 14 18 20 22 25 30 34 40
1.5
.5
-.5
1.5
.5
-.5
Stimulation frequency (Hz)M
ean
pow
er (
z-sc
ore)
**
*̂*̂
^̂
^̂ *̂*̂^̂ *̂*̂ *̂*̂ *̂*̂
Ext
race
llula
r Pot
entia
l (m
V)
Peri-stimulus Time (sec)
2
1
0
-1
-2
2
1
0
-1
-2
i
2
1
0
-1
-2
2
1
0
-1
-2-.5 0 .5 1 1.5 2 2.5 3 3.5-.5 0 .5 1 1.5 2 2.5 3 3.5
ii
2
0
3
1
-1
-2
-3
2
0
3
1
-1
-2
-3
2
0
3
1
-1
-2
-3
2
0
3
1
-1
-2
-3
i 20 Hz
Continuous
Mean integrated powerpulsed odorpulsed blank3s cont. odor
Peri-stimulusTime (sec)
Pro
babi
lity
0 0.2 0.4 0.6 0.8 1 1.2Time (sec)
00.10.20.3
Pro
babili
ty
0 .2 .4 .6 .8 1 1.2
0.1.2.3
Odo
r
A6A7A8A9
A10K6K7K8K9
K10B 10 20 30 40 50 10 20 30 40 50
Mea
n P
ower
(z-
scor
e) 1
.5
0
-.5
-1
Frequency (Hz)
i ii
Uni
ts R
espo
ndin
g (%
)10 14 18 20 22 25 30 34 40
Stimulation frequency (Hz)
50
40
30
20
10
0
stim
Oscillating stimulus results in oscillating unitary spiking from antennal lobe neurons up to ~40 Hz (extracellular)
Oscillating stimulus results in oscillating unitary spiking from antennal lobe neurons up to only ~15 Hz (intracellular)
1
0
-1
1
0
-1
10 20 30 40 5010 20 30 40 50Mea
n P
ow
er (
z-sc
ore)
Frequency (Hz)
10 20 30 40 5010 20 30 40 50Mea
n P
ow
er (
z-sc
ore)
1
0
-1
1
0
-1
Frequency (Hz)
Local interneuron
Projection neuron
GABAA disruption results in loss of oscillating responses from antennal lobe LFP and unitary spiking
10 20 30 40 465
50
40
30
20
10
0
50
40
30
20
10
0Uni
ts R
espo
ndin
g (%
)
Stimulation Frequency (Hz)
Loss of unitary oscillatory responses
10 20 30 40 46 57 7210 20 30 40 46 57 72
.8
.6
.4
.2
0
-.2
-.4
-.6
.8
.6
.4
.2
0
-.2
-.4
-.6Mea
n po
wer
(z-
scor
e)
Stimulation Frequency (Hz)
* * * * * * *
-.5 0 .5 1 1.5
100
80
60
40
20
0
100
80
60
40
20
0
Peri-stimulus Time (sec)M
ean
pow
er (
z-sc
ore)
Fre
quen
cy (
Hz)
i
ii
10
0
8
6
4
2
10
0
8
6
4
2
pre bicucullinepre
bicuculline
Reconstructing whole antennal lobe output responses by serial reconstruction
Virtual population responses from confirmed PN in response to an odor panel