The Development of the JAES LVI Calculator Version 3, rev 1a

Concept, Development, Implementation, and Operation of the LVI Calculator

ByJeff Darr

(407) 836-1439 jeffrey.darr@ocfl.net

Orange→County Environmental Protection Division

Constructs for JAES LVI Calculator

• Fore, L. 2007. Assessing the Biological Condition of Florida Lakes: Development of the Lake Vegetation Index (LVI)

• 2008 FDEP SOP LT 7500-5, 5.1- 5.8 Lake Vegetation Index Determination – Index Calculation

• Common Software (Accessibility) MS Excel® • Simple and Familiar Data Entry Process (Field

Data Sheet FD 9000-7)

New Features of Version 3• Single page Taxa list: Version 2 had two columns of taxa and data,

which sometimes made it difficult to find taxa. Version 3 has a single column of taxa and data.

• Automatic Taxa insertion: Version 2 required you to look up the taxa in the SOP, transcribe the CofC values, and determine what nativity status it was in order to choose the proper calculation formulae. Then you had to copy and replicate those formulae across all sample sections.

Version 3 only requires you to “Click” on the new taxa on the included worksheet, then press a button. Version 3 will automatically insert the new taxa in the appropriate row, and properly insert all the correct formulae, automatically across all sample sections.

• Worksheet Protection: Version 2 did not protect the calculation cells. Sometimes users would accidentally erase rows or columns that were calculating the score.

Version 3 worksheet protects the calculation and result cells. Users can only edit the cells that require data entry.

Data Sheet FD 9000-7

2, 5, 8, 11

3, 6, 9, 12

SPECIES C of C 1 2 3 4

Specimen collected (check)

Approx. water depth SPECIES C of C 1 2 3 4

Specimen collected (check)

Approx. water depth

Acer rubrum 4.65 Habenaria repens 4.58

Alternanthera philoxeroides(2) 0.00 Hydrilla verticillata(1) 0.00

Azolla caroliniana 1.81 Hydrochloa caroliniensis (Luziola fluitans)4.79

Baccharis glomeruliflora 6.12 Hydrocotyle umbellata 1.92

Bacopa caroliniana 5.31 Hygrophila polysperma(1) 0.00

Bacopa monnieri 4.49 Hypericum fasciculatum 7.27

Bidens laevis 7.19 Hypericum hypericoides 5.44

Bidens mitis 6.31 Hypericum myrtifolium 6.56

Blechnum serrulatum 7.15 Ilex cassine 7.66

Boehmeria cylindrica 5.91 Ipomoea aquatica(1) 0.00

Brachiaria mutica 0.00 Ipomoea sagittata 6.42

Brasenia schreberi 8.79 Itea virginica 7.09

Cabomba caroliniana 5.07 Juncus effusus 3.25

Carex albolutescens (C. longii) 3.47 Juncus marginatus 3.65

Carex verrucosa 7.97 Juncus megacephalus 5.70

Centella asiatica 1.92 Juncus repens 6.91

Cephalanthus occidentalis 7.27 Lachnanthes caroliana 3.76

Ceratophyllum demersum 4.16 Leersia hexandra 5.61

Ceratopteris thalictroides 2.93 Lemna minor 3.77

Chara spp. 3.90 Leucothoe racemosa 9.44

Cladium jamaicense 9.04 Limnobium spongia 4.79

Clethra alnifolia 5.63 Limnophila sessiliflora(2) 0.00

Cliftonia monophylla 5.63 Liquidamber styraciflua 5.56

Colocasia esculenta(1) 0.00 Ludwigia arcuata 5.32

Commelina diffusa 2.02 Ludwigia leptocarpa 3.47

Crineum americanum 8.67 Ludwigia octovalvis 4.09

Cyperus alternifolius(2) (C. involucratus)0.00 Ludwigia peruviana 0.62

Cyperus articulatus 6.64 Ludwigia repens 5.20

Cyperus haspan 5.68 Lyonia ferruginea 8.39

Cyperus lanceolatus 2.04 Lyonia ligustrina 8.67

Cyperus polystachyos 1.56 Lyonia lucida 7.06

Cyperus prolifer(2) 0.00 Magnolia virginiana 9.44

Cyperus surinamensis 2.03 Mayaca fluviatilis 8.45

Cyrilla racemiflora 5.20 Melaleuca quinquenervia(1) 0.00

Decodon verticillatus 7.80 Micranthemum glomeratum 5.85

Diodia virginiana 4.96 Micranthemum umbrosum 5.66

Echinochloa crusgalli 0.22 Mikania scandens 1.95

Eclipta alba none Mimosa pigra(1) 0.00

Egeria densa 0.49 Myrica cerifera 3.82

Eichhornia crassipes(1) 0.00 Myriophyllum aquaticum 0.67

Eleocharis baldwinii 2.82 Myriophyllum heterophyllum 4.77

Eleocharis cellulosa 7.80 Myriophyllum laxum 5.85

Eleocharis equisetoides 9.10 Myriophyllum pinnatum 5.85

Eleocharis interstincta 7.80 Myriophyllum spicatum(2) 0.00

Erechtites hieracifolia 1.37 Najas guadalupensis 5.07

Eriocaulon compressum 7.50 Najas marina 5.50

Eriocaulon decangulare 7.50 Najas minor 3.64

Eupatorium capillifolium 0.83 Nasturtium officinale 2.93

Fuirena breviseta 7.60 Nelumbo lutea 6.26

Fuirena scirpoidea 6.50 Nitella spp. 7.28

Gordonia lasianthus 9.03

Lake Vegetation Index Data Sheet (10-19-06)

Date:

STORET:

Waterbody:

Analyst:

County:

Signature:

Except where noted on sheet, species level identification required. Plants in bold=exotic. (1)= category 1 invasive (worst), (2)=category 2 invasive.

It is important to note dominant or co-dominant taxa (by areal extent) for each unit. Use "D" to denote dominant taxon, or "C" for the 2 co-dominant taxa.

Lake units sampled (circle one group): 1, 4, 7, 10Algal mats present (circle 1): yes, noLake water level (circle 1): high, normal, low

SPECIES C of C 1 2 3 4

Specimen collected (check)

Approx. water depth SPECIES C of C 1 2 3 4

Specimen collected (check)

Approx. water depth

Nuphar sp. 4.64 Solanum tampicense(1) 0.00

Nymphaea odorata 7.18 Sparganium americanum 6.50

Nymphoides aquatica 6.09 Spartina alterniflora 7.94

Nyssa sylvatica var. biflora 9.04 Spartina bakeri 5.98

Orontium aquaticum 8.39 Spirodela polyrhiza 2.95

Osmunda cinnamomea 6.44 Spirodela punctata none

Osmunda regalis 8.04 Taxodium ascendens 7.21

Panicum hemitomon 5.82 Taxodium distichum 7.21

Panicum repens(1) 0.00 Thalia geniculata 7.12

Paspalidium geminatum 6.36 Thelypteris palustris none

Paspalum distichum 5.54 Triadenum virginicum 8.16

Paspalum repens 6.69 Typha sp. 1.60

Paspalum urvillei 0.00 Utricularia biflora 6.37

Peltandra virginica 7.31 Utricularia floridana 6.34

Pennisetum purpureum(1) 0.00 Utricularia foliosa 6.44

Phragmites australis 4.39 Utricularia inflata 5.85

Pinus elliottii 4.21 Utricularia purpurea 6.50

Pistia stratioides(1) 0.00 Utricularia cornuta 7.46

Pluchea odorata 4.96 Utricularia radiata 6.01

Pluchea rosea 5.45 Valliseneria americana 7.28

Polygonum densiflorum 5.32 Websteria confervoides none

Polygonum hydropiperoides 4.02 Wedelia trilobata(2) 0.00

Polygonum punctatum 4.02 Wolffiella spp. 4.37

Pontederia cordata 5.38 Woodwardia virginica 6.5

Potamogeton diversifolius 7.15 Zizania aquatica 6.69

Potamogeton illinoensis 6.64 Zizaniopsis milacea 6.21

Potamogeton pusillus 7.80

Potemogeton pectinatus 7.80

Proserpinaca pectinata 5.50

Rhynchospora microcarpa 5.29

Rhynchospora microcephala 6.50

Rhynochospora tracyi 9.03

Ricca fluitans 4.64

Ricciocarpus natans 4.55

Ruppia maritima 7.24

Sabal palmetto 4.85

Sabatia grandiflora 7.09

Sacciolepis striata 5.35

Sagittaria kurziana 9.75

Sagittaria lancifolia 4.96

Sagittaria latifolia 6.50

Sagittaria graminea 5.53

Salix carolina 2.95

Salvinia minima 2.03

Sambucus canadensis 1.48

Sapium sebiferum(1) 0.00

Saururus cernuus 7.33

Schinus terebinthifolius(1) 0.00

Scirpus americanus 6.50

Scirpus californicus 6.01

Scirpus cubensis 3.77

Scirpus validus 5.55

JAES LVI Calculator Worksheet

JAES LVI All_Taxa WorksheetThis is the Second Worksheet Included with the JAES Calculator. It’s basically LT7500-1 with a column of calculations to figure out formulas.

Notice the Automatic “Add” Button on THIS sheet. This will be covered in detail later.

Closer Look Calculator Sheet – Upper Left

Closer Look Calculator Sheet – Lower Left

This is the only Entry the User will make

down here: “North” or “South”

Top Right of Form – Lake Sections

User Enters the 4 Sample sections from the Lake.

These 4 numbers will distribute themselves throughout the form automatically

Top Right of Form – Lake Sections

This Button will allow you to delete rows. First, you select the Taxon under column A, then you press this button.

This will be covered in detail later.

Enter Data in Section Column

User will enter a “1” for present, or a “C” for Co-dominant, or a “D” for dominant, for each section.

Scores are automatically calculated

Adding New Taxa

Adding Taxon Data

•Aeschynomene indica

At some point, the user will need to enter taxa that are not listed on the calculator. This calculator has two worksheets included in it. The first is the calculator worksheet, the second is the complete list of taxa from the FDEP SOP Table LT7500-1.

In our example here, the user wants to add Aeschynomene indica to the calculater worksheet. The next screens will show the steps on how to do this.

Adding Taxa: Step 1

User Clicks on the “All_Taxa” worksheet tab to reveal all the taxa from which to select.

Adding Taxa: Step 2

User then finds the desired taxon under column A, and “Clicks” on the taxon. (Just in Column A -- not the whole row)

Adding Taxa: Step 3

User then “Clicks” on “Add Row to Calculator” button.

Adding Taxa: Verifying Add

User will verify the Add by clicking YES. If taxon is incorrect, then user can click NO.

Adding Taxa: Complete

When you click the “Add” button, you will notice a lot of scrolling of the worksheets happening; this is Normal. When finished, you will notice the new taxon inserted alphabetically in the calculator. It will automatically have the correct formulae embedded into its row. You are finished with the Add.

Deleting Taxa

Deleting Taxa within a Protected Document

The JAES LVI Calculator worksheet is protected by password to keep unauthorized changes from happening to cells that calculate the score.

Consequently, the user cannot delete any rows within the worksheet without using the delete button included in the upper right corner of the calculator worksheet.

If we wanted to delete a row that we don’t want anymore in the calculator, we can use the delete button, and delete as many rows as we want, one at a time.

The following slides will show the easy 2 step process and verification to delete a row.

Deleting Taxa (Rows): Step 1

User finds the desired taxon (row) to delete and then “Clicks” on the taxon only. (Column A – not whole row)

Deleting Taxa (Rows): Step 2

User then “Clicks” on the “Delete Row” button in the upper right corner of the worksheet.

Deleting Taxa (Rows): Verify Delete

User then will Verify the Delete, by pressing Yes. If row or taxon is incorrect, then user can cancel delete by pressing NO.

Deleting Taxa (Rows): Complete

User then can inspect that the taxon (row) has been deleted. User is now finished with Delete.

Program Development• Embedded formulae• Index Calculation• Metric Calculation• Disclaimer• References

(This is all Technical and Legal Stuff)

Embedded Formulae

Embedded formulae – DominancedI = Dominant Indicator

=IF(ISTEXT(C10),1,0)

Reflects “1” if entry is a letter

Nat = Native Indicator

=IF(NOT(ISBLANK(C12)),1,0)

Reflects “1” if entry is Native

Embedded formulae – Native Indicator

Embedded formulae – FLEPPC Invasive Indicator

Inv = Invasive Indicator

=IF(NOT(ISBLANK(C11)),1,0)

Reflects “1” if entry is FLEPPC Invasive Exotic

Embedded formulae – Sensitivity IndicatorSens = Sensitivity Indicator

=IF(((NOT(ISBLANK(C128))*AND($B128>=7))),1,0) Reflects “1” if entry is Sensitive and present

Embedded formulae – Dominance CalculatorDom = Dominance Calculator

=IF(C129="D",$B129,IF(C129="C",$B129/2,0))

Reflects CofC value if dominant, CofC / 2 if Co-dominant

Index Calculation

Index Calculation – Nat/Inv/Sens/Dom

Percent Native – FDEP SOP LT7500-5.1

5.1 Calculate the % native taxa in a single sampling unit (pie slice) by dividing the number of native taxa by the total number of taxa in that sampling unit. Nativity status is determined by the Institute for Systematic Botany and is listed in Table 7500-1.

=IF(AE$127 = 0, 0,((AG124+E122)/AE$127)*100)Divide by zero Protection

N Col 1 N Col 2 Section Total * 100+ /

Percent FLEPPC Invasive – FDEP SOP LT7500-5.2

5.2 Calculate the % FLEPPC invasive taxa in a single sampling unit (pie slice) by adding the number of FLEPPC Category I and II taxa and then dividing by the total number of taxa in that sampling unit. Category I and II lists are maintained by FLEPPC, the Florida Exotic Pest Plant Council, and are listed in Table 7500-1.

=IF(AE$127 = 0, 0, ((AH124+F122)/AE$127)*100)

Divide by zero Protection

I Col 1 I Col 2 Section Total * 100+ /

Percent Sensitive – FDEP SOP LT7500-5.3

5.3 Calculate the % sensitive taxa in a single sampling unit by summing the number of taxa with a C of C (Coefficient of Conservatism) score >= 7 and then dividing by the total number of taxa in that sampling unit. Refer to Table 7500-1 for a list of C of C scores.

=IF(AE$127 = 0, 0, ((AI124+G122)/AE$127)*100) Divide by zero Protection

S Col 1 S Col 2 Section Total * 100+ /

C of C Dominance – FDEP SOP LT7500-5.4

5.4 Calculate the Dominant C of C for a single sampling unit by inserting the C of C for the dominant taxon or the average C of C for the 2 co-dominant taxa. If no dominant taxon was identified, this metric will be null, not zero. Refer to Table 7500-1 for a list of C of C scores.

=(AJ124+H122) CofC Col 1 CofC Col 2+

Missing C of C Scores

If a taxon does not have a C of C score, then it just will not be considered for the % Sensitive metric or have its C of C used for the dominant metric. It is very rare that a dominant taxa will not have an assigned C of C score.

Metric Calculations

Metric Scoring – FDEP SOP LT7500-5.5

5.5 Metric scoring rules derived from the 5th and 95th percentiles using the most recent visit from each lake including lakes from the original development data set. Metric scores less than 0 are set equal to 0; scores greater than 1 are set equal to 1.

(Fore L.S., 2007 pp 97)

SOUTH

NORTH

Closer Look…

Scoring Formulae

Native =IF(AG128-54>44,1,IF(AG128<54,0,(AG128-54)/44))

Invasive =IF((AH129>36),0,1-(AH129/36))

Sensitive =IF(AC140="South", IF(AI130>24,1,AI130/24), IF(AI130>37,1,AI130/37))

C of C =IF(AC140="South", IF(AJ131>5.8,1,AJ131/5.8), IF(AJ131>7.2,1,AJ131/7.2))

Over/Under flow protection

Native =IF(AG128-54>44,1,IF(AG128<54,0,(AG128-54)/44))

Invasive =IF((AH129>36),0,1-(AH129/36))

Sensitive =IF(AC140="South", IF(AI130>24,1,AI130/24), IF(AI130>37,1,AI130/37))

C of C =IF(AC140="South", IF(AJ131>5.8,1,AJ131/5.8), IF(AJ131>7.2,1,AJ131/7.2))

> 1 < 0

Metric Scoring – FDEP SOP LT7500-5.6,7

5.6 Average the results for the metrics within an individual sampling unit and multiply by 100. Note that you divide by 3 instead of 4 if the dominant (or co-dominant) C of C was not available. The result of this calculation must be between 0 and 100.

5.7 Average the LVI’s determined for all the sampling units (four units are sampled) to calculate the final LVI score for the lake.

Averaging Results Based on Existence of Dominance

(N + I + S + D) ÷ 3 or 4

Final Scoring – Metric Interpretation

Metric Scoring – FDEP SOP LT7500-5.8Aquatic life use

category

LVI

Range

Description

Category 1 “exceptional”

78–100 Nearly every macrophyte present is a species native to Florida, invasive taxa typically not found. About 30% of taxa present are identified as sensitive to disturbance and most taxa have C of C values >5.

Category 2 “healthy”

38–77 About 85% of macrophyte taxa are native to Florida; invasive taxa present. Sensitive taxa have declined to about 15% and C of C values average about 5.

Category 3 “impaired”

0–37 About 70% of macrophyte taxa are native to Florida. Invasive taxa may represent up to 1/3 of total taxa. Less that 10% of the taxa are sensitive and C of C values of most taxa are <4.

=IF(AD143>77,"Category I - EXCEPTIONAL",IF(AD143>37,"Category II - HEALTHY","Category III - IMPAIRED"))

Cooperative Agreement• End User Acknowledges that the LVI calculator is only a convenient field tool used for

instant results and is not a replacement for manual calculations or for formal submission to FDEP for official scoring.

• End User Recognizes and agrees that any score that would indicate a change in a status of a lake would require the data sheets be sent to FDEP for verification, and a manual calculation be done to check for accuracy.

• Owner of intellectual property (Orange County) and programmer (Jeff Darr) disclaims any liability for scores generated by calculator; users (Cooperative agencies) acknowledge that it is the user’s responsibility to verify accuracy of results.

• This software can only be distributed free of charge, and no portion thereof can be copied or used in any fee based software. Add and Delete Macro code is owned by programmer, developed independently of distributor and can only be distributed freely within this calculator software.

• End user cannot claim loss or damage to property as a result of execution of software. • End User agrees to always check the FDEP site for changes in Coefficient of

Conservatism (C of C) scores. It is the agency’s responsibility to check that all scoring rules and C of C values are official for the timeframe in which the LVI was performed.

Literature Cited

• Fore, L. 2007. Assessing the Biological Condition of Florida Lakes: Development of the Lake Vegetation Index (LVI)

• 2008 FDEP SOP LT 7500-5, 5.1- 5.8 Lake Vegetation Index Determination – Index Calculation

• 2008 FDEP Field Data Sheet FD 9000-7

• 2008 FDEP Table LT7500-1