CROATIAN PLANT BREEDING, SEED AND NURSERY PRODUCTION AND EUROPEAN INTEGRATION Šibenik, September 28 to 30, 2011 The effect of cereal seed treatment with the gastric pentadecapeptide BPC 157 Samobor Vesna 1 , Dijana Horvat 1 , D. Drmić 2 , S. Seiwerth 2 , P. Sikirić 2 and M. Jošt 3 1 College of Agriculture Križevci, Croatia 2 Department of Pharmacology and Pathology, Medical Faculty, University of Zagreb, Croatia 3 JOST Seed-Research, Križevci, Croatia
Transcript
CROATIAN PLANT BREEDING, SEED AND NURSERY PRODUCTION AND EUROPEAN INTEGRATIONŠibenik, September 28 to 30, 2011
The effect of cereal seed treatment with the gastric pentadecapeptide
BPC 157
Samobor Vesna1, Dijana Horvat1, D. Drmić2, S. Seiwerth2, P. Sikirić2 and M. Jošt3
1 College of Agriculture Križevci, Croatia2 Department of Pharmacology and Pathology, Medical Faculty,
University of Zagreb, Croatia3 JOST Seed-Research, Križevci, Croatia
Introduction
➲ This investigation is a continuation of earlier research on efficiency of cereal seed treatment in organic agriculture.
➲ The results of stone powder application on seed before sowing were published in Agronomy Journal 6/2008. andSjemenarstvo 27/2010.
The purpose of this preliminary investigation
Examining the effect of BPC 157 as cereal seed treatment agent in organic agriculture.
What is BPC 157?
➲ BPC 157 is an anti-ulcer peptidergic agent, proven in clinical trials to be both safe in inflammatory bowel disease and wound healing, with no toxicity being reported.
➲ It is soluble in water.
➲ In earlier (nonpublished) investigations it showed stimulating effect in extremely small concentrations (thousands of ppm) on growth and development of cultivated plants.
Material and methods➲ Investigations of the seed diseases infestation, germination
ability and plant vigor at rye (cv. Echo Kurz), wheat (cv. Divana) and corn (cv. Bijeli osmak) were conducted in replicated tests.
➲ Beside control variant, water solutions of BPC 157 were applied for seed treatment in the following concentrations:
● A = 125 ppm● B = 0.125 ppm● C = 0.000 125 ppm● D = 0.000 000 125 ppm
The international ISTA methods (NN 99/08.) were applied in tests.
Material and methods
➲ Before seed contamination tests (Petri-dishes) and germination (sand and roles of filter paper), the seed were for short time soaked in BPC 157 water solution and than air dried.
➲ Diseases attack were determined by microscope (count od spores expresed in %) after incubation in germination room:
- 24 hours on NUV light (320-420 nm) - 24 hours in refrigerator at -25 °C - 14 days on 18 °C (12 hours light - 12 hours dark)
Perti-dishes before and after test
Spores determination and count were done per each seed by stereomicroscope.
Infection intensity (%) of rye seed (cv. Echo Kurz) after treatment with
Infection intensity (%) of wheat seed (cv. Divana) after treatment with BPC 157
Tre
atm
en
t
Acre
mo
nie
la a
tra
Alt
ern
ari
a a
ltern
ata
Fu
sari
um
mo
nil
ifo
rme *
Tri
ch
od
erm
a h
arz
ian
um
Control 23 18 3 3 0 10 3
B 0 5 0 0 3 3 3
D 5 0 0 0 5 0 3
eliminated, and It is not pathogenic.
Bacte
riu
m
sp
p.
Cla
do
sp
ori
um
sp
.
Rh
izo
pu
s
sp
.
* Fusarium moniliforme appears when saprophite fungi are
Infection intensity (%) of corn seed (cv. Bijeli osmak) after treatment with BPC 157
T
rea
tme
nt
A
cre
mo
nie
lla a
tra
A
lte
rna
ria
alt
ern
ata
T
ric
ho
de
rma
ha
rzia
nu
m
Control 8 3 6 22 24 0 7 7 4
B 3 5 6 24 19 0 2 2 5
D 7 3 5 27 24 2 14 14 6
A
cre
mo
niu
m
sp
p.
C
lad
os
po
riu
m
sp
.
F
us
ari
um
sp
p.
N
igro
sp
ora
sp
.
P
en
icil
ium
s
p.
R
hiz
op
us
s
p.
Plant vigor of wheat (cv. Divana)- 14 days after start of germination
Germination and vigor of wheat - 14 days after start of germination
Treatment Germination -% Leaf length - cm Root length - cm
Control
B
D
* The values marked with the same letter are not significantly different at p=0.05
93.7 a 13.8 a 19.6 a
94.6 a 14.7 b 20.5 b
95.8 a 14.8 b 20.3 ab
Plant vigor of corn (cv. Bijeli osmak)- 14 days after start of germination
Germination and vigor of corn - 14 days after start of germination
Treatment Germination % Leaf length cm Root length cm
Control
B
D
* The values marked with the same letter are not significantly different at p=0.05
85.3 a 7.7 a 15.8 a
85.4 a 11.5 b 22.8 b
92.0 b 11.9 b 24.1 b
Conclusion
At presowing treatment of cereal seed pentadecapeptide BPC 157, in very small concentration (thousands of ppm), showed efficient suppression of pathogens on seed coat, especially on heavy infested seed.
Generally, seed germination was also improved, as well as plant vigor (leaf and root length of 14 days old seedlings).
Surprising findings was that the lowest concentration of pentadecapeptide BPC 157 (D variant) had the strongest effect.
BPC 157 could be suitable for seed protection in in organic agriculture.
Additional investigations of different concentrations and an application method are needed.
