The Effect of Exercise on O2 Consumption and CO2 Production

Effect of Exercise on 02 Consumption and CO2 Production

Design Outline

Hypothesis: Increased exercise/work will increase CO2 exhaled

IV: Amount of exercise

Levels of IV: recline, walking, stepping, high exercise (mostly anaerobic)

Trials: 27 27 27 27

DV: Amount of CO2 exhaled (micromoles) and pulse (bpm)

Constants: time exercised, time breathing, amount of H2OFocus Questions:

What’s the relationship between workload, pulse and CO2 production?

Why do different people produce different CO2 levels with the same workload?

Effect of Exercise on 02 Consumption and CO2 Production

Lab Chemistry – CO2 makes acid

• CO2 + H2O H2CO3 (acid)

• H2CO3 HCO3- + H+

• H+ determines pH (H+ = pH-- more acidic )

Effect of Exercise on 02 Consumption and CO2 Production

Lab Chemistry– Neutralizing the acid by titration

• NaOH Na+ + OH- (base)

• OH- + H+ H2O (acids and bases neutralize one another).

• With an indicator, solution will change color when neutral.

• Solution is neutral when volume NaOH = volume CO2.

• NaOH used is an accurate measure of CO2 in the solution.

Effect of Exercise on 02 Consumption and CO2 Production

Lab Protocol – (4) Exercise Levels

•rest-recline position-lay on floorlow exercise -walk down north hall onlymedium exercise –alternate leg step on chairshigh exercise – push ups, jumping jack, air squat (knee squats)

•3 minutes each

•Set-up breathing tube before you exercise.

•Do each test in order. One partner does all four tests, then the other partner does all four tests.

Effect of Exercise on 02 Consumption and CO2 Production

Lab Protocol – Collecting CO2

•Breathe normally through plastic tubing for one minute.

•Plug nose as you breathe through tube. Be careful not to breathe too hard and splash water.

•Begin breathing immediately after activity.

•Partner measures pulse for 30 seconds. (record as beats/minute)

Effect of Exercise on 02 Consumption and CO2 Production

Lab Protocol - Titration

•NaOH can burn-wear goggles.

•Measure 100 ml of distilled water with graduated cylinder.

•Add only 3 drops of indicator!

•Remember to record starting position of NaOH.

•Add NaOH slowly until it holds color for one full minute.