The effects of Preservatives and Alzheimer's diseaseCaroline Hsiao

Need

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Need

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Plaques

PlaquesTangles in Neuron

Alzheimer’s Disease

http://pampee.20gig.com/wp-content/uploads/2009/04/alzheimers-disease.jpg

http://www.alzheimer.ca/english/disease/whatisit-intro.htm

Alzheimer’s Disease

http://www.topnews.in/health/files/alzheimers-brain.jpg

Calcium propionate

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Age-Dependent Neurodegeneration and Alzheimer-Amyloid Plaque Formation in Transgenic Drosophila

Greeve, and Kretzschmar,

y-Secretase/presenilin inhibitors for Alzheimer’s disease phenocopy Notch mutations in Drosophila1By: MICCHELLI, and ESLER

Dissecting the pathological effects of human A40 and A42 in Drosophila: A potential model for Alzheimer’s disease

By: Iijima and Liu

Neuroserpin Binds A and Is a Neuroprotective Component of Amyloid Plaques in Alzheimer Disease

By: Kinghorn and Crowther

Purpose

Preservatives will cause an increase in the development of Alzheimer's

symptoms

Hypothesis

The purpose of this experiment was to find if preservatives have any effect on

the development of Alzheimer's disease.

Methodology

By increasing the levels of preservatives (Calcium propionate) given to drosophila models and also exposing those to certain levels of stress will begin to worsen the symptoms of Alzheimer’s and/or begin to develop the symptoms of Alzheimer’s

Increased levels of Calcium propionate and also environmental stress will lead to the development of the plates in drosophila and eventually lead to death due to

Alzheimer’s development.

Normal Drosophila fed normal food

Normal Drosophila fed food with Calcium propionate added

Data will be collected everyday over a 3 month period, a total of 5 trials will be run

Drosophila fed normal food exposed to stress

Drosophila exposed to stress fed food with Calcium propionate added

The means will be calculated and run through statistical analysis (one way ANOVA followed by Scheffe Posthoc test)

BudgetItem Vender Item

NumberQuantity

Description

Price Total Price

Drosophila VialsCarolina Biology Catalog 173120 4 12 vials per order $7.50 $30.00

Drosophila FoodCarolina Biology Catalog 173200 1

Formula 4-24® Instant Medium, Plain, 1 L $6.95 $6.95

APP Drosophila

PBSFisher Sciences Education BP2438-4 5

A standard phosphate buffer $95.56 $477.80

acetoneCarolina Biology Catalog 841481 5

Clear, colorless, flammable liquid $6.50 $32.50

methanolCarolina Biology Catalog 874950 5 flammable $5.75 $28.75

formalin Carolina Biology Catalog 863463 5 flammable $21.95 $109.75

xyleneCarolina Biology Catalog 898741 5 flammable $10.60 $53.00

ethanolCarolina Biology Catalog 861261 5

denatured with isopropanol and methanol $7.95 $39.75

distilled waterCarolina Biology Catalog 858621 5 Distilled Water $7.25 $36.25

Serum Antibody SetCarolina Biology Catalog 202102 5

Goat antisera containing immunoglobulin $99.00 $495.00

Disposable Lab Pack: Carolina Biology Catalog 632920 1

50 Slides,50 Plastic Coverslips,Lens Paper Booklet $11.75 $11.75

i-Brite Plus Neuromics SF40000 5anti-fade mounting media 10 ml Price not yet known Price not yet known

Calcium propionate honeyvill grains 27-816 5

discount 4 to 9: $89.29, 10 to 50: $84.59 $89.99 $89.29

Phosphate Buffer Solution

Carolina Biology Catalog 746864 5

0.1 M Phosphate Buffer Solution $8.50 $40

Do Ability

•All materials are orderable from school approved companies

•Drosophila can be kept alive with relative ease in school lab

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inhibitors for Alzheimer’s disease phenocopy Notch mutations in Drosophila, FASEB, 3/09/2003, volume 17, pgs 79-81

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