The Future Preparative Chromatography Here · 2007. 5. 7. · Part No.:00B-4436-P0-AX Mobile...

�

��

��

��

� ��

�

��

��

��

� ��

FirstCycle

The Future of Preparative Chromatography

is Here

�

��

��

��

� ��

�

��

��

��

� ��

Pat

ent

Pen

din

g

Preparative columns with guaranteed longer lifetime!

1,300thCycle

5

• Extended column lifetime

• Reproducibility: column-to-column

• Higher efficiency

• Improved peak shape

• Increased loadability

• Stability under high flow rates

Hydraulic Piston Compression

An advanced column packing and hardware design, Axia™ incorporates patent-pending Hydraulic Piston Compression technology to eliminate bed collapse as a source of failure in short preparative columns. Ideal bed density is custom calculated and automated for each support, chemistry, and column size. Computer control of the entire process assures

both proper bed density and uniformity. Using a single, controlled hydraulic compression, the piston assembly is locked in place without allowing the media to decompress or “relax,” thus maintaining media and column bed integrity. Recompression of the bed is not required, as it is for other packing methodologies.

Waters® OBD™ MediaSEM* of Waters® OBD™ inlet frit shows crushed media particles or fines at frit surface after packing.

Phenomenex® Axia™ Packed MediaSEM* of Axia™ inlet frit shows media largely intact at frit surface after packing.

The result is an improved, repeatable packing process, giving preparative chromatographers:

Axia Packing Process

See the technology in action! Please visit www.AxiaPrep.com

*The images are believed to be representative, but individual columns may vary.

Packing Piston

Media packed under ideal pressure

The packing piston head is integrated into the column and locked by the piston

retainers, so the pressure is never released.

Media is never allowed to relax, eliminating

voids and dramatically improving reproducibility,

column to column.

4 Phenomenex www.phenomenex.com

7

�

��

��

� �

�

�

�

�

�

�

��

��

��

��

��

��

��

��

��

��

�

��

�

��

��

��

��

��

��

��

��

��

Reproducible Column-to-Column EfficiencyAverage Efficiency (N) with Synergi™ 4µm Hydro-RP 100 x 21.2mm

Reproducible Column-to-Column Peak AsymmetryAverage Peak Asymmetry with Gemini™ 5µm C18 50 x 21.2mm

Expect an Excellent Column. Every Time.Consistent Quality. Column-to-Column. Batch-to-Batch.

%RSD %RSD

11.8

2.5

9.2

5.2

Gradient Lifetime Study

ConditionsColumns: Luna® 5µm C18(2) Axia™ Packed

Dimension: 50 x 21.2mmPart No.: 00B-4252-P0-AX

Mobile Phase: A: 0.5% TFA in Water B: 0.5% TFA in Acetonitrile

Gradient: Linear 95:5 to 5:95 (A/B) over 7 min, hold 3 min

Injection Volume: 500 μLFlow Rate: 30 mL/min

Temperature: AmbientDetection: UV @ 254nm

Sample: 1. Triprolidine 1.6 mg2. Methacycline 16 mg3. Amitriptyline 5.25 mg

In conventional slurry packing of short preparative columns, the packing process has several fundamental limitations including variability of media density leading to inconsistent column-to-column efficiency and peak asymmetry. With the new Axia™ – hydraulic piston compression technology – the correct slurry amount and packing pressure are automated to give not only higher efficiency and sharper peaks, but also drastically reduced column-to-column variability.

Under aggressive high-throughput conditions—gradient mobile phases, fast flow rates and 100% DMSO injections— Axia columns have shown over 1300 injections without signs of degradation or voiding!

Column Performance

Cycle Plates Asymmetry

1 3950 1.19150 3943 1.19300 3940 1.22450 4059 1.14600 4100 1.15900 4000 1.12

1040 3888 1.111130 3523 1.12

Overlay of 1, 800, 1300 chromatograms

Axia™ Gradient Lifetime Study

��

��

��

��

��

��

��

��

��

��

��

�

��

��

��

Density Comparison of Packed Beds

See page 11 for Axia™ stability under extreme flow rates and pressure!

See page 12 to extend Axia™ lifetime even further!

27% Improved Avg. Efficiency

13% Improved Avg. Peak Shape


9

Conditions

Columns: Gemini™ 10µm C18 Axia™ PackedDimensions: 50 x 21.2mm

Part No.: 00B-4436-P0-AXMobile Phase A: 95:5 50mM Ammonium Bicarbonate,

pH 10.0 / AcetonitrileB: 5:95 50mM Ammonium Bicarbonate, pH 10.0 / Acetonitrile

Gradient: 7 min 100% A to 100% B over 7 min, equilibrate for 3 min

Injection Volume: 500 μLFlow Rate: 30 mL/minDetection: UV @ 254nm


High Efficiency in a Preparative ColumnNow Possible When It’s Axia™ Packed!

Conditions(same for both columns except where noted)

Column: Synergi™ 4µm Hydro-RP Dimensions: 75 x 4.6mm

75 x 30mm Axia™ PackedPart Nos.: 00C-4375-E0

00C-4375-U0-AXHPLC Systems: Agilent 1100 (4.6mm ID)

Gilson Preparative System (30mm ID)Mobile Phase: Water/Acetonitrile (35:65)

Flow Rate: 1 mL/min (4.6mm ID) 43 mL/min (30mm ID)

Detection: UV @ 254nm Temperature: Ambient

Sample: 1. Uracil2. Acetophenone3. Benzene4. Toluene5. Naphthalene

��

�

� �

�

�

��

�

�

��

�

� �

�

�

�

�

Imagine higher compound purity and recovery!

App ID 15932

App ID 15931

Selectivity Options. You Decide.

� ��

�

�

�

�

�

�

�

��

Low pHLuna® 5µm C18(2) 50 x 21.2mm

High pHGemini™ 10µm C18 50 x 21.2mm

Conditions

Column: Luna® 5µm C18(2) Axia™ PackedDimension: 50 x 21.2mm

Part No.: 00B-4252-P0-AXMobile Phase: A: 0.5% TFA in Water

B: 0.5% TFA in AcetonitrileGradient: Linear 95:5 to 5:95 (A/B) over 7

min, hold 3 minInjection Volume: 500 μL

Flow Rate: 30 mL/min Detection: UV @ 254nm


Phenomenex packs the same analytical-grade media in preparative columns allowing easy scale-up from analytical. Achieve analytical-like efficiency in preparative columns for:

• Narrower peaks

• Smaller fraction volumes

• Faster dry-down time

• Improved resolution

With high surface areas, Phenomenex medias — Gemini (375m2/g), Luna (400m2/g) and Synergi (475m2/g) — maximize

loading capabilities. Use the selection tree below to select the best media for your targeted purification.

pH SelectivityAlternate Peak Elution Order

Stationary Phase Selectivity

Column Selection Tree

Basic Compounds?Yes No

Luna C8(2) or C18(2)

Synergi Max-RP

Polar?Yes No

Aromatic?Yes No

Synergi Polar-RP

Luna Phenyl-Hexyl Synergi Polar-RP

Synergi Hydro-RP

Gemini C6-Phenyl (Basic mobile phase pH

recommended)

Gemini C18 (Basic mobile phase pH recommended)

Aromatic?Yes No

See page 10 to increase loading for basic compounds.

4.6mm ID

30mm ID


11

Use Axia Packed columns to increase the throughput of your purifications. Conventional slurry packed columns tend to void or leak when drastically increasing the stress on the column. The Hydraulic Piston Compression

technology has shown the high density packed media withstands excessively high flow rates with virtually no sign of degradation after 520 cycles at 2x the normal operating conditions.

ConditionsColumn: Luna® 10µm C18(2) Axia™ Packed


Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Water/Acetonitrile (25:75)

Gradient: Linear 93:7 (A/B) to 100% B over 5 min

Injection Volume: 420µLFlow Rate: 60mL/min

Temperature: AmbientDetection: UV @ 270 nm

Sample: 1. Nadolol2. Metoprolol3. Propranolol

Low pH


Dimensions: 50 x 21.2mm Part No.: 00B-4252-P0-AX

Mobile Phase: A: 0.5% TFA in Water, pH 2.0B: Acetonitrile

Gradient: 95:5 to 5:95 (A/B) over 4 minutesTemperature: Ambient

Flow rate: 30 mL/minDetection: UV @ 254 nm

Sample: 1. Propranolol2. Diphenhydramine

High pH

ConditionsColumn: Gemini™ 5µm C18 Axia™ Packed

Dimensions: 50 x 21.2mm Part No.: 00B-4435-P0-AX

Mobile Phase: A: 50 mM Ammonium Bicarbonate, pH 10.0 B: Acetonitrile

Gradient: 95:5 to 5:95 (A/B) over 5 minutesTemperature: Ambient

Flow rate: 30 mL/minDetection: UV @ 254 nm

Sample: 1. Propranolol2. Diphenhydramine

��

��

��

��

��

��

��

� �

�

�

�

�

�

� � ��

�

��

��

�

�

�

�

�

� � ��

��

�

��

��

��

��

��

�

�

��

��

��

��

��

��

�

�

�

�

� � � ��

��

�

��

��

��

��

��

��

�

�

� �

�

� � � ��

� �

�

�

�

� � � ��

�

��

��

��

��

��

��

Increase Sample Load

pH 2

Axia Stability Under High Flow Rates

Cycle 1

Cycle 520

pH 10

pH 10

Increase pH for basic compounds

Double the load

60mg load

30mg load

30mg load

In Reversed Phase chromatography, compounds retain better when neutral. With the advent of pH stable (1-12) medias such as Gemini C18 and C6-Phenyl, improving retention and resolution of your basic compounds at high

pH is now possible without compromising column lifetime. Under these conditions you can easily double or triple the loading compared to your current low pH purifications.

60mL/min on a 21.2mm ID!

Lifetime study under extreme conditions


13



Mobile Phase: A: 0.1% TFA in WaterB: 0.1% TFA in Water/Acetonitrile (25:75)

Gradient: Linear 93:7 (A/B) to 100% B over 5 minutesInjection Volume: 420µL

Flow Rate: 60mL/minTemperature: Ambient

Detection: UV@ 270 nmSample: 1. Nadolol

2. Metoprolol3. Propranolol

� � � � � � ��

�

��

��

��

��

��

��

��

� �

�

� � � � � � ��

�

��

��

��

��

��

��

��

��

�

�

�

�

�

�

�

�

� � ��

��

�

��

��

��

��

��

Protect Your Axia™ Investment Order InformationSecurityGuard™ PREP System

• Extends column lifetime by as much as 5x

• Protects column from samples that precipitate out of solution

• Protects column from mobile phase and sample particulates

• Cost-effective system to protect your preparative column investment

• Stable and leak-free up to 60mL/min

Forced Degradation Study

Injection 1: Axia Packed column with SecurityGuard™ PREP Cartridge

Injection 240: Axia Packed column with SecurityGuard™ PREP Cartridge

Injection 241: Axia Packed column after removing SecurityGuard™ PREP cartridge

SecurityGuard™ PREP

PREP Holder

PREP Cartridge

1

240

241

Time to change the PREP cartridge

Original column performance maintained with

SecurityGuard™ PREP


SecurityGuard™ PREP Holder KitPart No. Description Unit Price

AJ0-7838 SecurityGuard™ PREP HPLC Guard Cartridge Holder Kit, 21.2mm ID, includes SS column coupler and 2 spare O-rings

ea

SecurityGuard™ PREP Cartridges Part No. Description Dimensions Price

AJ0-7846 Gemini C18 15 x 21.2mm ID 1 @

3 @

5 @AJ0-7839 C18 15 x 21.2mm ID

AJ0-7840 C8 15 x 21.2mm ID

AJ0-7845 Polar-RP 15 x 21.2mm ID

AJ0-7843AQ C18 (for Synergi Hydro-RP)

15 x 21.2mm ID

AJ0-7841 Phenyl 15 x 21.2mm ID

AJ0-7842C12 (for Synergi Max-RP)

15 x 21.2mm ID

AJ0-7229 Silica 15 x 21.2mm ID

AJ0-8162 NH2 15 x 21.2mm ID

In-line FiltersUse between the pump and the injector to protect your column and system from mobile phase contaminants.

Part No. Description Unit Price

AF0-7866 HPLC PREP Column In-Line Filter, S.S., 2.0μm Porosity x 21.2mm dia. ea

Replacement Parts and AccessoriesPart No. Description Unit Price

AF0-7867 Replacement In-Line Filter Disks, S.S., 2.0µm Porosity x 21.2mm dia.

ea

AQ0-7876 PREP Coupler, SS, 1⁄16 in OD x 0.020 in ID x 50mm L, with Fittings

ea

AQ0-7877 PREP Replacement O-Rings, 1 in OD x 1⁄7 in ID x 116 in CS, Fluorocarbon

2/pk

AT0-0465 Capillary S.S. Tubing, 0.20 in ID x 0.062 in (1⁄16 in) OD x 10cm L 5/pk

AT0-0466 Capillary S.S. Tubing, 0.20 in ID x 0.062 in (1⁄16 in) OD x 20cm L 5/pk

If Axia™ packed columns do not provide LONGER LIFETIME when used with SecurityGuard PREP, as compared to a competing column of the same particle size, phase and dimensions, send in your com-parative data and the column within 45 days for a full refund.

Order InformationAxia™ Packed Columns

SecurityGuard™ HPLC guard cartridge system order information on page 13.

OBD is a trademark of Waters® Corp. Luna and Polar-RP are registered trademarks of Phenomenex, Inc. in the USA and other countries. Gemini, Synergi, Axia and SecurityGuard are trademarks of Phenomenex. Phenomenex is in no way affliated with Waters Corp. © 2006 Phenomenex, Inc. All rights reserved.

test


50 x 21.2 mm Part No. Price

Gemini 5µm C18 00B-4435-P0-AX

Gemini 10µm C18 00B-4436-P0-AXNEW Gemini 5µm C6-Phenyl 00B-4444-P0-AX

Luna 5µm C18(2) 00B-4252-P0-AX

Luna 10µm C18(2) 00B-4253-P0-AX

Luna 5µm C8(2) 00B-4249-P0-AX

Luna 5µm Phenyl-Hexyl 00B-4257-P0-AXNEW Luna 5µm Silica 00B-4274-P0-AX

Synergi 4µm Max-RP 00B-4337-P0-AX

Synergi 4µm Hydro-RP 00B-4375-P0-AX

Synergi 4µm Polar-RP 00B-4336-P0-AX


NEW Gemini 5µm C18 00C-4435-P0-AXNEW Luna 5µm C18(2) 00C-4252-P0-AX


Gemini 5µm C18 00D-4435-P0-AX

Gemini 10µm C18 00D-4436-P0-AXNEW Gemini 5µm C6-Phenyl 00D-4444-P0-AX

Luna 5µm C18(2) 00D-4252-P0-AX

Luna 10µm C18(2) 00D-4253-P0-AX

Luna 5µm C8(2) 00D-4249-P0-AX

Luna 5µm Phenyl-Hexyl 00D-4257-P0-AXNEW Luna 5µm NH2 00D-4378-P0-AXNEW Luna 5µm Silica 00D-4274-P0-AX

Synergi 4µm Max-RP 00D-4337-P0-AX

Synergi 4µm Hydro-RP 00D-4375-P0-AX

Synergi 4µm Polar-RP 00D-4336-P0-AX

Synergi 10µm Max RP 00D-4350-P0-AX

50 x 30 mm Part No. Price

Gemini 5µm C18 00B-4435-U0-AX

Gemini 10µm C18 00B-4436-U0-AXNEW Gemini 5µm C6-Phenyl 00B-4444-U0-AX

Luna 5µm C18(2) 00B-4252-U0-AX

Luna 5µm C8(2) 00B-4249-U0-AX

Luna 5µm Phenyl-Hexyl 00B-4257-U0-AX

Luna 10µm C18(2) 00B-4253-U0-AXNEW Luna 5µm Silica 00B-4274-U0-AX

Synergi 4µm Max-RP 00B-4337-U0-AX

Synergi 4µm Hydro-RP 00B-4375-U0-AX

Synergi 4µm Polar-RP 00B-4336-U0-AX


Gemini 5µm C18 00C-4435-U0-AXNEW Gemini 5µm C6-Phenyl 00C-4444-U0-AX

Luna 5µm C18(2) 00C-4252-U0-AX

Luna 5µm C8(2) 00C-4249-U0-AX

Luna 5µm Phenyl-Hexyl 00C-4257-U0-AX

Synergi 4µm Max-RP 00C-4337-U0-AX

Synergi 4µm Hydro-RP 00C-4375-U0-AX

Synergi 4µm Polar-RP 00C-4336-U0-AX


Gemini 5µm C18 00D-4435-U0-AX

Gemini 10µm C18 00D-4436-U0-AXNEW Gemini 5µm C6-Phenyl 00D-4444-U0-AX

Luna 5µm C18(2) 00D-4252-U0-AX

Luna 10µm C18(2) 00D-4253-U0-AX

Luna 5µm C8(2) 00D-4249-U0-AX

Luna 5µm Phenyl-Hexyl 00D-4257-U0-AXNEW Luna 5µm Silica 00D-4274-U0-AX

Synergi 4µm Max-RP 00D-4337-U0-AX

Synergi 4µm Hydro-RP 00D-4375-U0-AX

Synergi 4µm Polar-RP 00D-4336-U0-AX

Synergi 10µm Max-RP 00D-4350-U0-AX

4186

_I

��

��

��

��

��

��

��

��

��

��

��

��

��

��

��

��

��

��

��

��

��

��

Date post:	05-Sep-2020
Category:	Documents
Upload:	others
View:	0 times
Download:	0 times

The Future Preparative Chromatography Here · 2007. 5. 7. · Part No.:00B-4436-P0-AX Mobile...

Documents